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Membrane Protein Expression (membrane + protein_expression)

Distribution by Scientific Domains
Medical Sciences40%
Life Sciences40%

Selected Abstracts

Pure quadriceps myopathy in two sisters

EUROPEAN JOURNAL OF NEUROLOGY, Issue 4 2003
I. Mahjneh
The authors carried out a clinical, laboratory and muscle computed tomographgy CT follow-up study of 18,21 years on two sisters affected by quadriceps myopathy (QM). The onset in the fourth decade was a weakness in the thighs. During the follow-up study, the patients showed only vasti muscles involvement, normal creatine kinase (CK) levels, myopathic muscle biopsy and electromyography (EMG) and normal membrane protein expression on immunocytochemical analysis. Therefore, all muscle pathologies known to have quadriceps involvement as a leading feature have been ruled out. We conclude that our patients have pure QM with probable autosomal recessive inheritance. [source]

Examination of membrane protein expression in Paracoccus denitrificans by two-dimensional gel electrophoresis

JOURNAL OF BASIC MICROBIOLOGY, Issue 1 2004
Pavel Bouchal
The well-known metabolic versatility of the soil bacterium Paracoccus denitrificans poses a challenge for modern proteomic approaches. We describe here improved preparation conditions that allow good separation and quantitative analyses of hundreds of membrane or periplasmic proteins. To illustrate this optimized procedure, the results of a screening for membrane proteins associated predominantly with aerobic or anaerobic (denitrifying) modes of growth are presented. [source]

Advances and challenges in membrane protein expression

AICHE JOURNAL, Issue 4 2007
A. James Link
First page of article [source]

The RNA chaperone Hfq is essential for the virulence of Salmonella typhimurium

MOLECULAR MICROBIOLOGY, Issue 1 2007
Alexandra Sittka
Summary The RNA chaperone, Hfq, plays a diverse role in bacterial physiology beyond its original role as a host factor required for replication of Q, RNA bacteriophage. In this study, we show that Hfq is involved in the expression and secretion of virulence factors in the facultative intracellular pathogen, Salmonella typhimurium. A Salmonella hfq deletion strain is highly attenuated in mice after both oral and intraperitoneal infection, and shows a severe defect in invasion of epithelial cells and a growth defect in both epithelial cells and macrophages in vitro. Surprisingly, we find that these phenotypes are largely independent of the previously reported requirement of Hfq for expression of the stationary phase sigma factor, RpoS. Our results implicate Hfq as a key regulator of multiple aspects of virulence including regulation of motility and outer membrane protein (OmpD) expression in addition to invasion and intracellular growth. These pleiotropic effects are suggested to involve a network of regulatory small non-coding RNAs, placing Hfq at the centre of post-transcriptional regulation of virulence gene expression in Salmonella. In addition, the hfq mutation appears to cause a chronic activation of the RpoE-mediated envelope stress response which is likely due to a misregulation of membrane protein expression. [source]

B-cell chronic lymphocytic leukaemia cells show specific changes in membrane protein expression during different stages of cell cycle

BRITISH JOURNAL OF HAEMATOLOGY, Issue 4 2007
Fiona Bennett
Summary The proliferating component in chronic lymphocytic leukaemia (CLL) is usually small (<1%) and restricted to a specific micro-environmental niche. To characterize the proliferating component, CLL cells from bone marrow or lymph nodes of 23 patients were assessed for expression of up to 66 surface antigens in combination with nuclear Ki-67/MCM6. Ki-67 expression was associated with step-wise increases in CD23/CD95/CD86/CD39/CD27 and decreases in CD24/CD69/CXCR4/CXCR5. Ki-67+ cells showed increased CD38 expression, but with considerable inter-patient variability: in some cases Ki-67 expression was only detectable in CD38, CLL cells. The results suggest continuous re-entry into the cell cycle as no distinct stem cell pool was detectable. [source]