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Major Task (major + task)
Selected AbstractsResponsible alcohol service: lessons from evaluations of server training and policing initiativesDRUG AND ALCOHOL REVIEW, Issue 3 2001TIM STOCKWELL Abstract Responsible alcohol service programmes have evolved in many countries alongside a general increase in the availability of alcohol and a greater focus on the prevention of alcohol-related road crashes. They also recognize the reality that a great deal of high-risk drinking and preventable harm occurs in and around licensed premises or as drinkers make their way home. Early US efficacy studies of programmes which trained managers and barstaff to limit customers' levels of intoxication and prevent drink driving showed promise. Studies of effectiveness of these programmes in the wider community, and in the absence of the enforcement of liquor laws, found little benefit. The data will be interpreted as suggesting that, in reality, skills deficits in the serving of alcohol are not a significant problem compared with the motivational issue for a commercial operation of abiding by laws that are rarely enforced and which are perceived as risking the goodwill of their best customers. Australian, UK and US experiences with liquor law enforcement by police will be discussed along with outcomes from the Australian invention of Alcohol Accords, informal agreements between police, licensees and local councils to trade responsibly. It will be concluded that the major task involved in lifting standards of service and preventing harm is to institutionalize legal and regulatory procedures which impact most on licensed premises. A number of strategies are suggested also for creating a political and social climate which supports the responsible service of alcohol and thereby supports the enactment and enforcement of appropriate liquor laws. [source] Historical review of sample preparation for chromatographic bioanalysis: pros and consDRUG DEVELOPMENT RESEARCH, Issue 3 2007Min S. Chang Abstract Sample preparation is a major task in a regulated bioanalytical laboratory. The sample preparation procedure significantly impacts assay throughput, data quality, analysis cost, and employee satisfaction. Therefore, selecting and optimizing an appropriate sample preparation method is essential for successful method development. Because of our recent expertise, this article is focused on sample preparation for high-performance liquid chromatography with mass spectrometric detection. Liquid chromatography with mass spectrometric detection (LC-MS) is the most common detection technique for small molecules used in regulated bioanalytical laboratories. The sample preparation technologies discussed are pre-extraction and post-extraction sample processing, protein precipitation (PPT), liquid,liquid extraction (LLE), offline solid-phase extraction (SPE), and online solid-phase extraction. Since all these techniques were in use for more than two decades, numerous applications and variations exist for each technique. We will not attempt to categorize each variation. Rather, the development history, a brief theoretical background, and selected references are presented. The strengths and the limitations of each method are discussed, including the throughput improvement potential. If available, illustrations from presentations at various meetings by our laboratory are used to clarify our opinion. Drug Dev Res 68:107,133, 2007. ©2007 Wiley-Liss, Inc. [source] Comparative study of odour and GC-olfactometric profiles of selected essential oilsFLAVOUR AND FRAGRANCE JOURNAL, Issue 6 2001Anupama Kamath Abstract The market demand for novel foods and beverages is increasing. The blending of flavour notes to create an exotic one, to satisfy the tastes and fantasies of the modern generation, may soon become a major task for researchers. In order to achieve this, it is essential to study the single flavourant thoroughly, and the dominant and subtle notes in them, prior to blending. In the present study, profiling of four essential oils, viz. lemongrass, rosemary, geranium and davana, were carried out by two methods, GC-olfactometry (GC,O) and per se odour profile by trained panelists. The attributes of odour analysis and GC,O were compared using spider-web plots. The study showed that although some odour notes perceived by sniffing the oils directly and those perceived by sniffing the effluent of GC at different retention times (RTs) varied, the dominant notes were similar. The odour profiling per se depicts the overall odour perceived, while the GC,O aromagrams represent the picture of odour notes of fractionated volatiles at the respective retention times. The results of the study gives an in-depth description of odour notes in the essential oil and helps the technologist to blend them to achieve the required flavour notes. Thus, the study finds its application in the development of designer beverages containing essential oils or blends of oils with the desired odour characteristics. Copyright © 2001 John Wiley & Sons, Ltd. [source] Developing a new cost-efficient control strategy for an actual confectionery plant through the combined exploitation of experimental and numerical analysisINTERNATIONAL JOURNAL OF ENERGY RESEARCH, Issue 6 2003Piero Lunghi Abstract Achieving energy absorption reductions while improving indoor air quality is a major task when designing new air conditioning systems. A cost-effective way to improve energy efficiency without compromising the thermal comfort consists of developing better control. In the present work, an extensive experimental campaign has been coupled with a theoretical analysis with an effective approach. A simulation tool has been implemented and, through its predictions, an efficient control strategy has been developed in a system that resulted in significant energy savings and environmental benefits. Copyright © 2003 John Wiley & Sons, Ltd. [source] Where will pathogen inactivation have the greatest impact?ISBT SCIENCE SERIES: THE INTERNATIONAL JOURNAL OF INTRACELLULAR TRANSPORT, Issue 1 2007T. Hervig Blood safety has always been a major task in transfusion medicine. A strategy to obtain this aim should include donor education, donor selection, and testing of blood donations. Pathogen inactivation adds another level of safety. In the fractionation industry, pathogen inactivation methods are mandatory. Several countries also use pathogen-inactivated plasma , from pools or single donors. Concerning the cellular blood components, there is still no method available for red cell concentrates, whereas methods for platelet concentrates are available in some countries and others are in the pipeline for commercialization. The efficiency of the ,old' methods to increase blood safety and the costs of the methods seem to be major obstacles for the introduction of the systems. There are also concerns on product quality and loss of volume during the inactivation process. As the importance of pathogen inactivation is largest in countries with blood donors who carry infections it is impossible to protect against, either due to high incidence of the infection or due to shortage of tests, cost will be a major question when pathogen inactivation is considered. Pathogen inactivation of red cell concentrates will also be a necessity. When pathogen inactivation methods are available for all blood components, they will have great impact to protect the patients in countries where a high percentage of the population is infected by agents transmissible through blood transfusion, and in all situations to protect against new pathogens and ,old' pathogens that become more virulent. The total risk of contracting infectious diseases through blood transfusion will probably be important when implementation of new methods for pathogen inactivation is considered. [source] Cell death and differentiation in the development of the endocardial cushion of the embryonic heartMICROSCOPY RESEARCH AND TECHNIQUE, Issue 5 2002Eltyeb Abdelwahid Abstract The transformation of the endocardial cushion into valves and septa is a critical step in cardiac morphogenesis as it initiates the development of the four-chambered heart. This transformation results from a region-specific balance between cellular proliferation, apoptosis, and differentiation. The development of the form and structure of the endocardial cushion is accompanied by precise patterns of abundant cell death having the morphological features of programmed cell death (apoptosis), which plays an important role in the elimination of redundant cells and in changes of phenotypic composition during histogenesis. Apoptosis is an essential process in morphogenesis as it balances mitosis in renewing tissues. It is controlled by one or more genetic programs that kill the targeted cell. However, the causes, role, and regulation of apoptosis in the developing endocardial cushion still remain to be determined. The clarification of the role of the apoptosis regulatory genes constitutes a major task in future studies of cell death in the developing heart. This new molecular histology of heart development awaits further experiments to clarify the interactive mechanisms that act to ensure the sculpting of the endocardial cushion into valves and septa by determining the size of the cushion cell populations. The relation between the expression of different factors and the modifications of the cushion region during cardiac development are reviewed. In addition, we review and summarize information on molecules identified in our experiments that imply the activity of a number of essential genes coinciding with the key steps in generating the overall architecture of the heart. We correlate their temporal and spatial expression with their proposed roles. Microsc. Res. Tech. 58:395,403, 2002. © 2002 Wiley-Liss, Inc. [source] Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheatPLANT BREEDING, Issue 1 2000J. Liu Abstract Breeding durable resistance to pathogens and pests is a major task for modern plant breeders and pyramiding different resistance genes into a genotype is one way of achieving this. Three powdery mildew resistance gene combinations, Pm2+Pm4a, Pm2+Pm21, Pm4a+Pm21 were successfully integrated into an elite wheat cultivar ,Yang047,. Double homozygotes were selected from a small F2 population with the help of molecular markers. As the parents were near-isogenic lines (NILs) of ,Yang158,, the progenies showed good uniformity in morphological and other non-resistance agronomic traits. The present work illustrates the bright prospects for the utilization of molecular markers in breeding for host resistance. [source] Stem cell-based composite tissue constructs for regenerative medicineBIOTECHNOLOGY & BIOENGINEERING, Issue 3 2005Mohamed N. Rahaman Abstract A major task of contemporary medicine and dentistry is restoration of human tissues and organs lost to diseases and trauma. A decade-long intense effort in tissue engineering has provided the proof of concept for cell-based replacement of a number of individual tissues such as the skin, cartilage, and bone. Recent work in stem cell-based in vivo restoration of multiple tissue phenotypes by composite tissue constructs such as osteochondral and fibro-osseous grafts has demonstrated probable clues for bioengineered replacement of complex anatomical structures consisting of multiple cell lineages such as the synovial joint condyle, tendon-bone complex, bone-ligament junction, and the periodontium. Of greater significance is a tangible contribution by current attempts to restore the structure and function of multitissue structures using cell-based composite tissue constructs to the understanding of ultimate biological restoration of complex organs such as the kidney or liver. The present review focuses on recent advances in stem cell-based composite tissue constructs and attempts to outline challenges for the manipulation of stem cells in tailored biomaterials in alignment with approaches potentially utilizable in regenerative medicine of human tissues and organs. © 2005 Wiley Periodicals, Inc. [source] The impact of cytomorphology, cytogenetics, molecular genetics, and immunophenotyping in a comprehensive diagnostic workup of myelodysplastic syndromesCANCER, Issue 19 2009Ulrike Bacher MD Abstract BACKGROUND: Because of limited reproducibility of morphologic features, the morphological categorization of initial myelodysplastic syndromes (MDS) cases remains a major task in a diagnostic setting. METHODS: To further evaluate the role of additional diagnostic methods for suspected early MDS, the authors analyzed 1965 cases with unclear cytopenia where at least cytomorphology and immunophenotyping were performed in parallel, combined with cytogenetics and molecular genetics. RESULTS: In 353 patients, both methods diagnosed malignant/nonmalignant disease other than MDS, and 557 patients had MDS-refractory anemia with excess of blasts/chronic myelomonocytic leukemia. The remaining 1055 patients (53.7%), where early MDS/reactive cytopenia had to be assumed, were categorized into 6 groups depending on cytomorphology/immunophenotyping results for or against MDS. In 659 of 1055 cases (62.4%) with suspected initial MDS, cytomorphology and immunophenotyping were concordant in the categorization of MDS/non-MDS. Cytogenetics, available in 951 of 1055 patients, revealed the highest frequency of aberrant karyotypes when both cytomorphology and immunophenotyping proposed MDS (63 of 227; 27.8%). But also in the groups where either cytomorphology or immunophenotyping showed evidence of MDS, aberrant karyotypes were found in 6% to 14% of patients. Even when both morphology and immunophenotyping showed no MDS, 11 of 208 (5.3%) had cytogenetic aberrations. RUNX1/AML1 mutation screening was positive in 15% in the latter group. NRAS, MLL -PTD, NPM1, and JAK2V617F were detected in low frequencies, confirming MDS diagnosis in the respective cases. CONCLUSIONS: This report outlines the power of a combined diagnostic approach for suspected initial cases of MDS including immunophenotyping, cytogenetics, and molecular genetics with selected markers in addition to cytomorphology. Diagnostic algorithms should be developed, and immunophenotyping should be further validated for this specific indication. Cancer 2009. © 2009 American Cancer Society. [source] Lamina propria dendritic cells: For whom the bell TOLLs?EUROPEAN JOURNAL OF IMMUNOLOGY, Issue 6 2008Maria Rescigno PhD. Abstract One of the major tasks of the mucosal immune system is to discriminate between dangerous and harmless antigens that are encountered daily at mucosal sites. In the gastrointestinal tract, immune cells have to tolerate food antigens and commensal microbes but at the same time have to induce a prompt response against invasive pathogens, when needed. In this issue of the European Journal of Immunology, it is shown that intestinal dendritic cell (DC) populations can be distinguished based on the expression level of Toll-like receptors (TLR) and on the response of these TLR to their microbial ligands. DC either do not express TLR or they express them but respond in a non-inflammatory mode. In this commentary, these findings are discussed in the context of available knowledge on lamina propria DC. See accompanying article http://dx.doi.org/10.1002/eji.200737909 [source] How the parallel channels of the retina contribute to depth processingEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 5 2007Peter H. Schiller Abstract Reconstructing the third dimension in the visual scene from the two dimensional images that impinge on the retinal surface is one of the major tasks of the visual system. We have devised a visual display that makes it possible to study stereoscopic depth cues and motion parallax cues separately or in concert using rhesus macaques. By varying the spatial frequency of the display and its luminance and chrominance, it is possible to selectively activate channels that originate in the primate retina. Our results show that (i) the parasol system plays a central role in processing motion parallax cues; (ii) the midget system plays a central role in stereoscopic depth perception at high spatial frequencies, and (iii) red/green colour selective neurons can effectively process both cues but blue/yellow neurons cannot do so. [source] Integrating artificial intelligence into on-line statistical process controlQUALITY AND RELIABILITY ENGINEERING INTERNATIONAL, Issue 1 2003Ruey-Shiang Guh Abstract Statistical process control (SPC) is one of the most effective tools of total quality management, the main function of which is to monitor and minimize process variations. Typically, SPC applications involve three major tasks in sequence: (1) monitoring the process, (2) diagnosing the deviated process and (3) taking corrective action. With the movement towards a computer integrated manufacturing environment, computer based applications need to be developed to implement the various SPC tasks automatically. However, the pertinent literature shows that nearly all the researches in this field have only focussed on the automation of monitoring the process. The remaining two tasks still need to be carried out by quality practitioners. This project aims to apply a hybrid artificial intelligence technique in building a real time SPC system, in which an artificial neural network based control chart monitoring sub-system and an expert system based control chart alarm interpretation sub-system are integrated for automatically implementing the SPC tasks comprehensively. This system was designed to provide the quality practitioner with three kinds of information related to the current status of the process: (1) status of the process (in-control or out-of-control). If out-of-control, an alarm will be signaled, (2) plausible causes for the out-of-control situation and (3) effective actions against the out-of-control situation. An example is provided to demonstrate that hybrid intelligence can be usefully applied for solving the problems in a real time SPC system. Copyright © 2003 John Wiley & Sons, Ltd. [source] | ||||||||||||||||