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Long-term Alterations (long-term + alteration)
Selected AbstractsMicrobeam synchrotron imaging of hairs from Ancient Egyptian mummiesJOURNAL OF SYNCHROTRON RADIATION, Issue 5 2003L. Bertrand Developments in microfocus synchrotron techniques have led to new results regarding the long-term alteration of archaeological samples of biological origin. Here, ancient hair samples from two Egyptian mummies have been analyzed using a conjunction of structural and elemental synchrotron methods. In this favored context of conservation, structural analysis revealed a remarkable preservation of keratin supramolecular organization at any observed length scale. Bulk keratin structure has therefore not been modified significantly over 2000 years. However, infrared spectroscopy indicated a partial disorganization of keratins close to the hair surface through polypeptide bond breakage. Elemental mapping showed a strongly heterogeneous distribution which can be related to mummification and cosmetic treatments. [source] Long-term growth of soybean at elevated [CO2] does not cause acclimation of stomatal conductance under fully open-air conditionsPLANT CELL & ENVIRONMENT, Issue 9 2006ANDREW D. B. LEAKEY ABSTRACT Accurately predicting plant function and global biogeochemical cycles later in this century will be complicated if stomatal conductance (gs) acclimates to growth at elevated [CO2], in the sense of a long-term alteration of the response of gs to [CO2], humidity (h) and/or photosynthetic rate (A). If so, photosynthetic and stomatal models will require parameterization at each growth [CO2] of interest. Photosynthetic acclimation to long-term growth at elevated [CO2] occurs frequently. Acclimation of gs has rarely been examined, even though stomatal density commonly changes with growth [CO2]. Soybean was grown under field conditions at ambient [CO2] (378 µmol mol,1) and elevated [CO2] (552 µmol mol,1) using free-air [CO2] enrichment (FACE). This study tested for stomatal acclimation by parameterizing and validating the widely used Ball et al. model (1987, Progress in Photosynthesis Research, vol IV, 221,224) with measurements of leaf gas exchange. The dependence of gs on A, h and [CO2] at the leaf surface was unaltered by long-term growth at elevated [CO2]. This suggests that the commonly observed decrease in gs under elevated [CO2] is due entirely to the direct instantaneous effect of [CO2] on gs and that there is no longer-term acclimation of gs independent of photosynthetic acclimation. The model accurately predicted gs for soybean growing under ambient and elevated [CO2] in the field. Model parameters under ambient and elevated [CO2] were indistinguishable, demonstrating that stomatal function under ambient and elevated [CO2] could be modelled without the need for parameterization at each growth [CO2]. [source] A role for glutamate transmission in addiction to psychostimulantsADDICTION BIOLOGY, Issue 3 2000Peter W. Kalivas Psychostimulant addiction results in the emergence of undesirable behaviors such as drug craving and paranoia. Using animal models of addiction the neurobiological substrates mediating these behaviors have been examined. Studies have focused on cellular adaptations within the motive circuit that contains the nucleus accumbens, ventral tegmental area, ventral pallidum and prefrontal cortex. While long-term alterations in dopamine transmission have been clearly characterized, more recent studies reveal that important neuroadaptations are also produced in glutamate transmission. This short review provides a description of these neuroadaptations and a discussion of how these psychostimulant-induced changes may synergize to elicit addiction-related behaviors. [source] Rapid and long-term alterations of hippocampal GABAB receptors in a mouse model of temporal lobe epilepsyEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 8 2003Andrea Straessle Abstract Alterations of ,-aminobutyric acid (GABA)B receptor expression have been reported in human temporal lobe epilepsy (TLE). Here, changes in regional and cellular expression of the GABAB receptor subunits R1 (GBR1) and R2 (GBR2) were investigated in a mouse model that replicates major functional and histopathological features of TLE. Adult mice received a single, unilateral injection of kainic acid (KA) into the dorsal hippocampus, and GABAB receptor immunoreactivity was analysed between 1 day and 3 months thereafter. In control mice, GBR1 and GBR2 were distributed uniformly across the dendritic layers of CA1,CA3 and dentate gyrus. In addition, some interneurons were labelled selectively for GBR1. At 1 day post-KA, staining for both GBR1 and GBR2 was profoundly reduced in CA1, CA3c and the hilus, and no interneurons were visible anymore. At later stages, the loss of GABAB receptors persisted in CA1 and CA3, whereas staining increased gradually in dentate gyrus granule cells, which become dispersed in this model. Most strikingly, a subpopulation of strongly labelled interneurons reappeared, mainly in the hilus and CA3 starting at 1 week post-KA. In double-staining experiments, these cells were selectively labelled for neuropeptide Y. The number of GBR1-positive interneurons also increased contralaterally in the hilus. The rapid KA-induced loss of GABAB receptors might contribute to epileptogenesis because of a reduction in both presynaptic control of transmitter release and postsynaptic inhibition. In turn, the long-term increase in GABAB receptors in granule cells and specific subtypes of interneurons may represent a compensatory response to recurrent seizures. [source] Post-impact structural crater modification due to sediment loading: An overlooked processMETEORITICS & PLANETARY SCIENCE, Issue 11 2007Filippos Tsikalas The analysis demonstrates that the geometry and the structural and stratigraphic relations of post-impact strata provide information about the amplitude, the spatial distribution, and the mode of post-impact deformation. Reconstruction of the original morphology and structure for the Mjølnir, Chicxulub, and Bosumtwi craters demonstrates the long-term subsidence and differential compaction that takes place between the crater and the outside platform region, and laterally within the crater structure. At Mjølnir, the central high developed as a prominent feature during post-impact burial, the height of the peak ring was enhanced, and the cumulative throw on the rim faults was increased. The original Chicxulub crater exhibited considerably less prominent peak-ring and inner-ring/crater-rim features than the present crater. The original relief of the peak ring was on the order of 420,570 m (currently 535,575 m); the relief on the inner ring/crater rim was 300,450 m (currently ,700 m). The original Bosumtwi crater exhibited a central uplift/high whose structural relief increased during burial (current height 101,110 m, in contrast to the original height of 85,110 m), whereas the surrounding western part of the annular trough was subdued more that the eastern part, exhibiting original depths of 43,68 m (currently 46 m) and 49,55 m (currently 50 m), respectively. Furthermore, a quantitative model for the porosity change caused by the Chesapeake Bay impact was developed utilizing the modeled density distribution. The model shows that, compared with the surrounding platform, the porosity increased immediately after impact up to 8.5% in the collapsed and brecciated crater center (currently +6% due to post-impact compaction). In contrast, porosity decreased by 2,3% (currently ,3 to ,4.5% due to post-impact compaction) in the peak-ring region. The lateral variations in porosity at Chesapeake Bay crater are compatible with similar porosity variations at Mjølnir crater, and are considered to be responsible for the moderate Chesapeake Bay gravity signature (annular low of ,8 mGal instead of ,15 mGal). The analysis shows that the reconstructions and the long-term alterations due to post-impact burial are closely related to the impact-disturbed target-rock volume and a brecciated region of laterally varying thickness and depth-varying physical properties. The study further shows that several crater morphological and structural parameters are prone to post-impact burial modification and are either exaggerated or subdued during post-impact burial. Preliminary correction factors are established based on the integrated reconstruction and post-impact deformation analysis. The crater morphological and structural parameters, corrected from post-impact loading and modification effects, can be used to better constrain cratering scaling law estimates and impact-related consequences. [source] Muscle biopsy substantiates long-term MRI alterations one year after a single dose of botulinum toxin injected into the lateral gastrocnemius muscle of healthy volunteers,MOVEMENT DISORDERS, Issue 10 2009A. Sebastian Schroeder MD Abstract Despite numerous clinical and experimental studies on botulinum toxin type A (BoNT/A), long-term alterations of muscle texture and fine structure following BoNT/A treatment have thus far not been studied in normal human skeletal muscle. After obtaining institutional review board approval, we performed a prospective, placebo-controlled, double-blinded follow-up study on two healthy adults using magnetic resonance imaging (MRI) and muscle biopsy to visualize long-term alterations after a single BoNT/A injection into the lateral head of the gastrocnemius muscle. MRI disclosed a high-signal-intensity pattern in short tau inversion recovery sequences, and a reduction of the cross-sectional area in the BoNT/A-injected, but not in the saline-injected contralateral control muscle (at 6 to 9 months in volunteer A: 73%, in B: 62%; at 12 months in A: 88%, and in B: 78%). Enzyme histochemistry, 12 months after injection, confirmed neurogenic atrophy of muscle fibers only in the BoNT/A-injected muscle. Electron microscopy revealed additional degenerative changes at the neuromuscular junction. The data confirm that MRI is a suitable tool to monitor the long-term effect of BoNT/A on skeletal muscle. Neurogenic muscle atrophy following a single BoNT/A injection should be taken into consideration when repeated BoNT/A injections into the same muscles are proposed. © 2009 Movement Disorder Society [source] Pre- and peri-natal environmental risks for attention-deficit hyperactivity disorder (ADHD): the potential role of epigenetic processes in mediating susceptibilityTHE JOURNAL OF CHILD PSYCHOLOGY AND PSYCHIATRY AND ALLIED DISCIPLINES, Issue 10 2008Jonathan Mill Attention-deficit hyperactivity disorder (ADHD) is a common childhood neurobehavioural disorder defined by symptoms of developmentally inappropriate inattention, impulsivity and hyperactivity. As is the norm for most psychiatric phenotypes, traditional aetiological studies have focused primarily on the interplay between genetic and environmental factors. It is likely that epigenetic factors, i.e., heritable, but reversible changes to genomic function that are independent of DNA sequence, are also important. It is known that epigenetic processes can be induced following exposure to a range of external factors, and thus provide a mechanism by which the environment can lead to long-term alterations in phenotype. In this article we hypothesise that epigenetic dysregulation may mediate the association observed between early-development environmental insults and ADHD. We propose that understanding the epigenetic processes involved in linking specific environmental pathogens to an increased risk for ADHD may offer new possibilities for preventative and therapeutic intervention. [source] | ||||||||||