API

Distribution by Scientific Domains
Distribution within Chemistry

Terms modified by API

  • api mellifera
  • api mellifera l
  • api mellifera l.

  • Selected Abstracts


    OpenUH: an optimizing, portable OpenMP compiler

    CONCURRENCY AND COMPUTATION: PRACTICE & EXPERIENCE, Issue 18 2007
    Chunhua Liao
    Abstract OpenMP has gained wide popularity as an API for parallel programming on shared memory and distributed shared memory platforms. Despite its broad availability, there remains a need for a portable, robust, open source, optimizing OpenMP compiler for C/C++/Fortran 90, especially for teaching and research, for example into its use on new target architectures, such as SMPs with chip multi-threading, as well as learning how to translate for clusters of SMPs. In this paper, we present our efforts to design and implement such an OpenMP compiler on top of Open64, an open source compiler framework, by extending its existing analysis and optimization and adopting a source-to-source translator approach where a native back end is not available. The compilation strategy we have adopted and the corresponding runtime support are described. The OpenMP validation suite is used to determine the correctness of the translation. The compiler's behavior is evaluated using benchmark tests from the EPCC microbenchmarks and the NAS parallel benchmark. Copyright © 2007 John Wiley & Sons, Ltd. [source]


    Distributed computing with Triana on the Grid

    CONCURRENCY AND COMPUTATION: PRACTICE & EXPERIENCE, Issue 9 2005
    Ian Taylor
    Abstract In this paper, we describe Triana, a distributed problem-solving environment that makes use of the Grid to enable a user to compose applications from a set of components, select resources on which the composed application can be distributed and then execute the application on those resources. We describe Triana's current pluggable architecture that can support many different modes of operation by the use of flexible writers for many popular Web service choreography languages. We further show, that the Triana architecture is middleware-independent through the use of the Grid Application Toolkit (GAT) API and demonstrate this through the use of a GAT binding to JXTA. We describe how other bindings being developed to Grid infrastructures, such as OGSA, can seamlessly be integrated within the current prototype by using the switching capability of the GAT. Finally, we outline an experiment we conducted using this prototype and discuss its current status. Copyright © 2005 John Wiley & Sons, Ltd. [source]


    Comparative evaluation of quality of doxycycline formulations registered in Estonia to those registered in the Russian Federation

    DRUG DEVELOPMENT RESEARCH, Issue 2 2008
    A. Meos
    Abstract The in vitro properties of four Estonian drug market (manufactured in Austria, Germany, and Finland) and four Russian Federation drug market (manufactured in Belarussia and Russian Federation) doxycycline formulations were evaluated using the estimation of the quantitative content and purity of the active pharmaceutical ingredient (API) and the dissolution test. Tolerance limits were set according to the European Pharmacopoeia (for the content and purity of the API) and USP (for the dissolution test) doxycycline monographs. All Estonian drug market doxycycline formulations complied with the tolerance limits in all tests and assays. Most of the Russian Federation drug market doxycycline formulations also passed the tolerance limits, with two minor exceptions: one formulation contained quantitatively API below the USP limit (83.7% instead of the 90%), but all the API was readily released in the dissolution test, the other formulation (capsules) released 80% of API in 39,min instead of 30,min. The general conclusion of the study is that despite some deviations, the Russian Federation drug market doxycycline formulations are comparable with those purchased from the Estonian drug market. Drug Dev Res 69: 58,68, 2008. © 2008 Wiley-Liss, Inc. [source]


    Rapid detection and identification of counterfeit of adulterated products of synthetic phosphodiesterase type-5 inhibitors with an atmospheric solids analysis probe

    DRUG TESTING AND ANALYSIS, Issue 2 2010
    Marian Twohig
    Abstract The market success of the three approved synthetic phosphodiesterase type-5 (PDE-5) inhibitors for the treatment of erectile dysfunction has led to an explosion in counterfeit versions of these drugs. In parallel a large market has developed for herbal products claimed to be natural alternatives to these synthetic drugs. The herbal products are heavily advertised on the internet and are freely available to purchase without prescription. Furthermore, adulteration of these supposed natural medicines is a very common and serious phenomenon. Recent reports have shown that the adulteration has extended to the analogues of the three approved synthetic PDE-5 inhibitors. An Atmospheric Solids Analysis Probe (ASAP) was used for the direct analysis of the counterfeit pharmaceuticals and herbal products. Using the ASAP combined with time-of-flight mass spectrometry (TOF MS) it was possible to detect fraudulent counterfeit tablets. The physical appearance of the pills resembled the pills from the original manufacturer but contained the wrong active pharmaceutical ingredient (API). Detecting adulteration for five herbal supplements marketed as natural alternatives to PDE-5 inhibitors was also possible using the ASAP. Three types of adulteration were found in the five samples: adulteration with tadalafil or sildenafil, mixed adulteration (tadalafil and sildenafil), and adulteration with analogues of these drugs. Copyright © 2010 John Wiley & Sons, Ltd. [source]


    Alcohol control policies and alcohol consumption by youth: a multi-national study

    ADDICTION, Issue 11 2009
    Mallie J. Paschall
    ABSTRACT Aims The study examined relationships between alcohol control policies and adolescent alcohol use in 26 countries. Design Cross-sectional analyses of alcohol policy ratings based on the Alcohol Policy Index (API), per capita consumption and national adolescent survey data. Setting Data are from 26 countries. Participants Adolescents (aged 15,17 years) who participated in the 2003 European School Survey Project on Alcohol and Other Drugs (ESPAD) or national secondary school surveys in Spain, Canada, Australia, New Zealand and the United States. Measurements Alcohol control policy ratings based on the API; prevalence of alcohol use, heavy drinking and first drink by age 13 based on national secondary school surveys; per capita alcohol consumption for each country in 2003. Analysis Correlational and linear regression analyses were conducted to examine relationships between alcohol control policy ratings and past 30-day prevalence of adolescent alcohol use, heavy drinking and having first drink by age 13. Per capita consumption of alcohol was included as a covariate in regression analyses. Findings More comprehensive API ratings and alcohol availability and advertising control ratings were related inversely to the past 30-day prevalence of alcohol use and prevalence rates for drinking three to five times and six or more times in the past 30 days. Alcohol advertising control was also related inversely to the prevalence of past 30-day heavy drinking and having first drink by age 13. Most of the relationships between API, alcohol availability and advertising control and drinking prevalence rates were attenuated and no longer statistically significant when controlling for per capita consumption in regression analyses, suggesting that alcohol use in the general population may confound or mediate observed relationships between alcohol control policies and youth alcohol consumption. Several of the inverse relationships remained statistically significant when controlling for per capita consumption. Conclusions More comprehensive and stringent alcohol control policies, particularly policies affecting alcohol availability and marketing, are associated with lower prevalence and frequency of adolescent alcohol consumption and age of first alcohol use. [source]


    Determination of linear response in the detection of mixtures of aroma compounds by atmospheric pressure ionization,mass spectrometry (API,MS)

    FLAVOUR AND FRAGRANCE JOURNAL, Issue 1 2008
    Roberto A. Buffo
    Abstract The linearity of atmospheric pressure ionization,mass spectrometry (API,MS) response in mixtures of aroma compounds was determined for a ,homogeneous' system (four esters) and a ,heterogeneous' system (five compounds of different chemical functionality). All combinations analysed within each system followed a saturation pattern as concentration increased. Total linear responses were determined by the linear behaviour of individual compounds. Ionization patterns within each mixture were explained by proton affinity data (i.e. the species' ability to accept protons), molecular reaction rates (defined by molecular weight), and product ion distribution (according to fragmentation of the corresponding parent ion). Copyright © 2007 John Wiley & Sons, Ltd. [source]


    Confirmatory factor analysis and recommendations for improvement of the Autonomy-Preference-Index (API)

    HEALTH EXPECTATIONS, Issue 3 2010
    Daniela Simon PhD Dipl Psych
    Abstract Objective, Validation of the German version of the Autonomy-Preference-Index (API), a measure of patients' preferences for decision making and information seeking. Methods, Stepwise confirmatory factor analysis was conducted on a sample of patients (n = 1592) treated in primary care for depression (n = 186), surgical and internal medicine inpatients (n = 811) and patients with minor trauma treated in an emergency department (n = 595). An initial test of the model was done on calculation and validation halves of the sample. Both local and global indexes-of-fit suggested modifications to the scale. The scale was modified and re-tested in the calculation sample and confirmed in the validation sample. Subgroup analyses for age, gender and type of treatment setting were also performed. Results, The confirmatory analysis led to a modified version of the API with better local and global indexes-of-fit for samples of German-speaking patients. Two items of the sub-scale, ,preference for decision-making', and one item of the sub-scale, ,preference for information seeking', showed very low reliability scores and were deleted. Thus, several global indexes-of-fit clearly improved significantly. The modified scale was confirmed on the validation sample with acceptable to good indices of fit. Results of subgroup analyses indicated that no adaptations were necessary. Discussion and conclusions, This first confirmatory analysis for a German-speaking population showed that the API was improved by the removal of several items. There were theoretically plausible explanations for this improvement suggesting that the modifications might also be appropriate in English and other language versions. [source]


    Predictive model for the outcome of infliximab therapy in Crohn's disease based on apoptotic pharmacogenetic index and clinical predictors

    INFLAMMATORY BOWEL DISEASES, Issue 4 2007
    Tibor Hlavaty
    Abstract Background: Infliximab (IFX) is an effective therapy for refractory luminal and fistulizing Crohn's disease (CD). Predictors of response could improve selection of patients with a higher probability of favorable outcomes and could improve the safety profile. We aimed to develop a predictive model for the response to infliximab in CD. Methods: Genetic and clinical data collected in a previous pharmacogenetic study of apoptosis genes were analyzed using SAS Enterprise miner modeling software and SPSS 12.0. We proposed a novel apoptotic pharmacogenetic index (API) with a score ranging from 0 (low apoptotic response) to 3 (high apoptotic response) and subsequently developed a decision tree model. Results: Response and remission rates significantly increased with API score (P = 0.005 in the group of patients with luminal CD, P = 0.02 in the group of patients with fistulizing CD). Patients with an API , 1 (n = 59) had the lowest response and remission rates in both the luminal CD (50% and 39.5%, respectively) and fistulizing CD (61.9% and 28.6%, respectively) groups, compared to those with an API of 2 (n = 158), whose response and remission rates were 73.8% and 56.1%, respectively, in the luminal CD group and 85.7% and 44.9%, respectively, in the fistulizing CD group; and those with an API of 3 (n = 10), whose response and remission rates were 100% and 85.7%, respectively, in the luminal CD group and 100% and 0% in the fistulizing CD group. Response in patients with an API , 1 was significantly influenced by concurrent azathioprine therapy in the luminal CD (21.4% versus 78.9%, P < 0.001) and in the fistulizing CD (46.6% versus 100%, P = 0.04) groups. In patients with an API of 2, we saw an interaction with age older than 40 years and location of disease (response 52.2% versus 83.9%, P = 0.008) in the luminal CD group and with baseline CRP greater than 5 mg/L (73.9% versus 93.9%, P = 0.04) in the fistulizing CD group. Conclusions: From our newly proposed apoptotic pharmacogenetic index and clinical predictors, we developed a model for prediction of low, medium, and high responses to the first infusion of IFX in patients with CD. Further studies are needed to confirm the hypothesis generated by our study. (Inflamm Bowel Dis 2007) [source]


    Chemical and microbiological quality of Garris, Sudanese fermented camel's milk product

    INTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 3 2006
    Abdel Moneim El-Hadi Sulieman
    Summary In the present study, some of the chemical and microbiological characteristics of garris, a Sudanese traditionally fermented camel's milk product, were investigated. The chemical analyses included, pH, titrable acidity and ethanol contents. A total of 100 strains of lactic acid bacteria (LAB) were isolated from twenty samples of traditionally fermented household garris. The selected isolates were phenotypically characterized by their ability to ferment 49 carbohydrates using API 50 CHL kits and additional biochemical tests. LAB dominated the microflora of garris samples, and the major genera were Lactobacillus (74%), followed by Lactococcus (12%), Enterococcus (10%) and Leuconostocs (4%). The most predominant Lactobacillus species were identified as Lactobacillus paracasei ssp. paracasei (64 strains), L. fermentum (seven strains) and only three strains as L. plantarum. Most strains produced the enzymes that are relevant to cultured dairy product processing. The Lactococcus species were identified as Lactococcus lactis. The average pH value of the samples was 4.42 ± 0.21. The pH values were accompanied with increasing of titrable acidity which averaged 1.72 ± 0.04%. The relatively high amounts of ethanol detected in all samples (average 1.40 ± 0.03%) together with the high yeasts counts (6.0 ± 0.53 log10 cfu mL,1), indicated that the fermentation process of garris is a yeast-lactic fermentation. [source]


    Enhancing multimedia streaming over existing wireless LAN technology using the Unified Link Layer API

    INTERNATIONAL JOURNAL OF NETWORK MANAGEMENT, Issue 5 2007
    Tim Farnham
    This paper examines how multimedia streaming scenarios can be enhanced by cross-layer interaction, and in particular link performance information and configuration options provided by the recently developed Unified Link Layer API (ULLA). It provides results of an experimental implementation developed for this purpose in a wireless LAN (WLAN) environment. Multimedia streaming is an application that is gaining in popularity for mobile devices and in particular mobile Internet-based content broadcasting is rapidly emerging as a key feature on mobile devices. In these scenarios, the wireless link (last hop) is normally the performance bottleneck due to the dynamic and limited capacity of the wireless medium. The use of ULLA in this context can provide the ability to tailor the video transmission to the wireless link performance and also to configure the links in response to performance problems or environmental changes. For this purpose the focus of multimedia streaming has been on WLAN link technology and dynamic adaptation (i.e., dynamic channel selection and video transcoding) using a dynamic resource reservation overlay protocol. Copyright © 2007 John Wiley & Sons, Ltd. [source]


    Cytotoxic Bacillus spp. belonging to the B. cereus and B. subtilis groups in Norwegian surface waters

    JOURNAL OF APPLIED MICROBIOLOGY, Issue 5 2004
    Ø. Østensvik
    Abstract Aims:, To investigate the presence and numbers of Bacillus spp. spores in surface waters and examine isolates belonging to the B. cereus and B. subtilis groups for cytotoxicity, and to discuss the presence of cytotoxic Bacillus spp. in surface water as hazard identification in a risk assessment approach in the food industry. Methods and Results:, Samples from eight different rivers with variable degree of faecal pollution, and two drinking water sources, were heat shocked and examined for the presence of Bacillus spp. spores using membrane filtration followed by cultivation on bovine blood agar plates. Bacillus spp. was present in all samples. The numbers varied from 15 to 1400 CFU 100 ml,1. Pure cultures of 86 Bacillus spp. isolates representing all sampling sites were characterized using colony morphology, atmospheric requirements, spore and sporangium morphology, and API 50 CHB and API 20E. Bacillus spp. representing the B. cereus and B. subtilis groups were isolated from all samples. Twenty-one isolates belonging to the B. cereus and B. subtilis groups, representing eight samples, were screened for cytotoxicity. Nine strains of B. cereus and five strains belonging to the B. subtilis group were cytotoxic. Conclusions:, The presence of cytotoxic Bacillus spp. in surface water represents a possible source for food contamination. Filtration and chlorination of surface water, the most common drinking water treatment in Norway, do not remove Bacillus spores efficiently. This was confirmed by isolation of spores from tap water samples. Significance and Impact of the Study:, Contamination of food with water containing low numbers of Bacillus spores implies a risk for bacterial growth in foods. Consequently, high numbers of Bacillus spp. may occur after growth in some products. High numbers of cytotoxic Bacillus spp. in foods may represent a risk for food poisoning. [source]


    Isolation and Characterization of Lactobacillus Species Having Potential for Use as Probiotic Cultures for Dogs

    JOURNAL OF FOOD SCIENCE, Issue 3 2007
    S. McCoy
    ABSTRACT:, The need to control pathogenic microorganisms in the intestinal tract of dogs is a growing concern. There is interest in using probiotics such as species of Lactobacillus to help control canine intestinal infections. For successful use as a probiotic, the bacterial species should be of canine intestinal origin since these species exhibit host specificity. Serial dilutions of freshly voided dog feces were plated on Lactobacillus selection (LBS) agar to isolate the cultures. Isolates were identified based on Gram stain, catalase test, and fermentation patterns using API 50 CH kits. All potential isolates were compared for bile resistance based on relative ability to grow in broth containing 0.3% Oxgall, the ability to inhibit Salmonella Typhimurium in associative broth cultures, and the production of reuterin. Of the lactobacilli isolated, Lactobacillus reuteri was the dominant species. However, some cultures of L. acidophilus also were isolated. We found variations among the isolates of L. reuteri and L. acidophilus with respect to bile tolerance. In general, isolates of L. reuteri appeared to be more bile resistant than were isolates of L. acidophilus. There were also variations in the ability to inhibit growth of S. Typhimurium. Some isolates of L. reuteri produced reuterin while others did not. [source]


    Distinguishing N -oxide and hydroxyl compounds: impact of heated capillary/heated ion transfer tube in inducing atmospheric pressure ionization source decompositions

    JOURNAL OF MASS SPECTROMETRY (INCORP BIOLOGICAL MASS SPECTROMETRY), Issue 6 2004
    Dilrukshi M. Peiris
    Abstract In the pharmaceutical industry, a higher attrition rate during the drug discovery process means a lower drug failure rate in the later stages. This translates into shorter drug development time and reduced cost for bringing a drug to market. Over the past few years, analytical strategies based on liquid chromatography/mass spectrometry (LC/MS) have gone through revolutionary changes and presently accommodate most of the needs of the pharmaceutical industry. Among these LC/MS techniques, collision induced dissociation (CID) or tandem mass spectrometry (MS/MS and MSn) techniques have been widely used to identify unknown compounds and characterize metabolites. MS/MS methods are generally ineffective for distinguishing isomeric compounds such as metabolites involving oxygenation of carbon or nitrogen atoms. Most recently, atmospheric pressure ionization (API) source decomposition methods have been shown to aid in the mass spectral distinction of isomeric oxygenated (N -oxide vs hydroxyl) products/metabolites. In previous studies, experiments were conducted using mass spectrometers equipped with a heated capillary interface between the mass analyzer and the ionization source. In the present study, we investigated the impact of the length of a heated capillary or heated ion transfer tube (a newer version of the heated capillary designed for accommodating orthogonal API source design) in inducing for-API source deoxygenation that allows the distinction of N -oxide from hydroxyl compounds. 8-Hydroxyquinoline (HO-Q), quinoline- N -oxide (Q-NO) and 8-hydroxyquinoline- N -oxide (HO-Q-NO) were used as model compounds on three different mass spectrometers (LCQ Deca, LCQ Advantage and TSQ Quantum). Irrespective of heated capillary or ion transfer tube length, N -oxides from this class of compounds underwent predominantly deoxygenation decomposition under atmospheric pressure chemical ionization conditions and the abundance of the diagnostic [M + H , O]+ ions increased with increasing vaporizer temperature. Furthermore, the results suggest that in API source decompostion methods described in this paper can be conducted using mass spectrometers with non-heated capillary or ion transfer tube API interfaces. Because N-oxides can undergo in-source decomposition and interfere with quantitation experiments, particular attention should be paid when developing API based bioanalytical methods. Copyright © 2004 John Wiley & Sons, Ltd. [source]


    Tuning compounds for electrospray ionization/in-source collision-induced dissociation and mass spectra library searching

    JOURNAL OF MASS SPECTROMETRY (INCORP BIOLOGICAL MASS SPECTROMETRY), Issue 9 2001
    Wolfgang Weinmann
    Abstract Tuning compounds for positive and negative electrospray ionization (ESI) were tested for the tuning of in-source collision-induced dissociation (ESI/CID) with three types of SCIEX API instruments (API 365, 2000 and 3000) in the single-quadrupole mode. The vacuum interfaces of these instruments differ slightly in geometry, but the principles of ionization and solvent evaporation by nebulizer and curtain gases, orifice and skimmer are identical. For comparison of in-source CID, breakdown curves of haloperidol, paracetamol, metronidazole and metamizole were acquired by increasing the orifice voltages. The API 2000 and 3000 required higher orifice voltages than did the API 365 to induce a similar degree of fragmentation of the protonated or deprotonated molecules to characteristic fragment ions. This increase of orifice voltage could be demonstrated with each of the four compounds tested by a shift of the maxima of the breakdown curves to higher orifice voltages. A procedure with three collision energy (CE) levels for drug identification with a mass spectra library set up with an API 365 therefore required an adjustment of the orifice voltages to higher values when being transferred to an API 2000 or API 3000. The corresponding orifice voltages for the three instruments were 20/50/80 V (API 365), 30/90/130 V (API 2000) and 40/80/120 V (API 3000). However, a change in orifice voltage of ±10 V (with the API 2000 and 3000) hardly influenced the fit values of a library search for each single CE level. For adjusting orifice voltages with different instruments, a tuning procedure with haloperidol and paracetamol is presented. With this tuning procedure an ESI/CID mass spectra library set up for API 365 and API 150 could also be used for drug identification with an API 2000 and an API 3000 with good library search results. Copyright © 2001 John Wiley & Sons, Ltd. [source]


    Biowaiver monographs for immediate release solid oral dosage forms: Doxycycline hyclate,,

    JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 4 2010
    E. Jantratid
    Abstract Literature data relevant to the decision to allow a waiver of in vivo bioequivalence (BE) testing for the approval of immediate release (IR) solid oral dosage forms containing doxycycline hyclate are reviewed. According to the Biopharmaceutics Classification System (BCS), doxycycline hyclate can be assigned to BCS Class I. No problems with BE of IR doxycycline formulations containing different excipients and produced by different manufacturing methods have been reported and hence the risk of bioinequivalence caused by these factors appears to be low. Doxycycline has a wide therapeutic index. Further, BCS-based dissolution methods have been shown to be capable of identifying formulations which may dissolve too slowly to generate therapeutic levels. It is concluded that a biowaiver is appropriate for IR solid oral dosage forms containing doxycycline hyclate as the single Active Pharmaceutical Ingredient (API) provided that (a) the test product contains only excipients present in doxycycline hyclate IR solid oral drug products approved in the International Conference on Harmonization (ICH) or associated countries; and (b) the comparator and the test products comply with the BCS criteria for "very rapidly dissolving" or, alternatively, when similarity of the dissolution profiles can be demonstrated and the two products are "rapidly dissolving.". © 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 99: 1639,1653, 2010 [source]


    Characterization of amorphous API:Polymer mixtures using X-ray powder diffraction

    JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 11 2008
    Ann Newman
    Abstract Recognizing limitations with the standard method of determining whether an amorphous API,polymer mixture is miscible based on the number of glass transition temperatures (Tg) using differential scanning calorimetry (DSC) measurements, we have developed an X-ray powder diffraction (XRPD) method coupled with computation of pair distribution functions (PDF), to more fully assess miscibility in such systems. The mixtures chosen were: dextran,poly(vinylpyrrolidone) (PVP) and trehalose,dextran, both prepared by lyophilization; and indomethacin,PVP, prepared by evaporation from organic solvent. Immiscibility is detected when the PDF profiles of each individual component taken in proportion to their compositions in the mixture agree with the PDF of the mixture, indicating phase separation into independent amorphous phases. A lack of agreement of the PDF profiles indicates that the mixture with a unique PDF is miscible. In agreement with DSC measurements that detected two independent Tg values for the dextran,PVP mixture, the PDF profiles of the mixture matched very well indicating a phase separated system. From the PDF analysis, indomethacin,PVP was shown to be completely miscible in agreement with the single Tg value measured for the mixture. In the case of the trehalose,dextran mixture, where only one Tg value was detected, however, PDF analysis clearly revealed phase separation. Since DSC can not detect two Tg values when phase separation produces amorphous domains with sizes less than approximately 30 nm, it is concluded that the trehalose,dextran system is a phase separated mixture with a structure equivalent to a solid nanosuspension having nanosize domains. Such systems would be expected to have properties intermediate to those observed for miscible and macroscopically phase separated amorphous dispersions. However, since phase separation has occurred, the solid nanosuspensions would be expected to exhibit a greater tendency for physical instability under a given stress, that is, crystallization, than would a miscible system. © 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97:4840,4856, 2008 [source]


    Wide-ranging molecular mobilities of water in active pharmaceutical ingredient (API) hydrates as determined by NMR relaxation times

    JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 10 2008
    Sumie Yoshioka
    Abstract In order to examine the possibility of determining the molecular mobility of hydration water in active pharmaceutical ingredient (API) hydrates by NMR relaxation measurement, spin,spin relaxation and spin,lattice relaxation were measured for the 11 API hydrates listed in the Japanese Pharmacopeia using pulsed 1H-NMR. For hydration water that has relatively high mobility and shows Lorentzian decay, molecular mobility as determined by spin,spin relaxation time (T2) was correlated with ease of evaporation under both nonisothermal and isothermal conditions, as determined by DSC and water vapor sorption isotherm analysis, respectively. Thus, T2 may be considered a useful parameter which indicates the molecular mobility of hydration water. In contrast, for hydration water that has low mobility and shows Gaussian decay, T2 was found not to correlate with ease of evaporation under nonisothermal conditions, which suggests that in this case, the molecular mobility of hydration water was too low to be determined by T2. A wide range of water mobilities was found among API hydrates, from low mobility that could not be evaluated by NMR relaxation time, such as that of the water molecules in pipemidic acid hydrate, to high mobility that could be evaluated by this method, such as that of the water molecules in ceftazidime hydrate. © 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97:4258,4268, 2008 [source]


    Biowaiver monographs for immediate release solid oral dosage forms: ethambutol dihydrochloride,,

    JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 4 2008
    C. Becker
    Abstract Literature data relevant to the decision to allow a waiver of in vivo bioequivalence (BE) testing for the approval of immediate release (IR) solid oral dosage forms containing ethambutol dihydrochloride as the only active pharmaceutical ingredient (API) are reviewed. Ethambutol dihydrochloride is a Biopharmaceutics Classification System (BCS) Class III drug with permeability properties approaching the border between BCS Class I and III. BE problems of ethambutol formulations containing different excipients and different dosages forms have not been reported and hence the risk of bioinequivalence caused by excipients is low. Ethambutol has a narrow therapeutic index related to ocular toxicity. However, as long as the prescribers' information of the test product stipulates the need for regular monitoring of ocular toxicity, the additional patient risk is deemed acceptable. It is concluded that a biowaiver can be recommended for IR solid oral dosage forms provided that the test product (a) contains only excipients present in ethambutol IR solid oral drug products approved in ICH or associated countries, for instance as presented in this paper, (b) complies with the criteria for "very rapidly dissolving" and (c) has a prescribers' information indicating the need for testing the patient's vision prior to initiating ethambutol therapy and regularly during therapy. © 2007 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97:1350,1360, 2008 [source]


    Biowaiver monographs for immediate release solid oral dosage forms: Amitriptyline hydrochloride,

    JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 5 2006
    R.H. Manzo
    Abstract Literature data relevant to the decision to allow a waiver of in vivo bioequivalence (BE) testing for the approval of immediate release (IR) solid oral dosage forms containing amitriptyline hydrochloride are reviewed. Its therapeutic uses, its pharmacokinetic properties, the possibility of excipient interactions and reported BE/bioavailability (BA) problems are also taken into consideration. Literature data indicates that amitriptyline hydrochloride is a highly permeable active pharmaceutical ingredient (API). Data on the solubility according to the current Biopharmaceutics Classification System (BCS) were not fully available and consequently amitriptyline hydrochloride could not be definitively assigned to either BCS Class I or BCS Class II. But all evidence taken together, a biowaiver can currently be recommended provided that IR tablets are formulated with excipients used in existing approved products and that the dissolution meets the criteria defined in the Guidances. © 2006 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 95:966,973, 2006 [source]


    Measurement of low-dose active pharmaceutical ingredient in a pharmaceutical blend using frequency-domain photon migration

    JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 3 2004
    Tianshu Pan
    Abstract Frequency-domain photon migration (FDPM) measurements of time-dependent light propagation are conducted to provide the powder absorbance for quantitative prediction of terazosin as the active pharmaceutical ingredient (API) in a low-dose (0.72 wt %) oral tablet formulation. Calibration of the FDPM-derived powder absorbance at discrete wavelengths of 514, 650, 687, and 785 nm was performed for API contents ranging between 0 and 1.5 wt % in mixtures showing maximum sensitivity at 650 nm. The relative standard deviation (RSD) of FDPM absorption coefficient measurement at 650 nm in a well-mixed 1.08 wt % terazosin blend was <1.6%, of which no more than 0.12% arose from FDPM instrumental error and the remainder was attributable to the complete-random-mixture model. The applicability of FDPM as an on-line sensor for powder-blending operations was further evaluated by analyzing grab samples taken directly from five locations of a 2-cu-ft Gallay blender at intervals of 5 min within the blending process. FDPM results indicate that homogeneity was largely achieved in the first 10 min, during which the RSD of API content across five sampling locations decreased from 27% to 8%, and the RSD decreased to 5% after 25 min of blending. Evolution of homogeneity within the blending process assessed through FDPM measurements was fit to the first-order model of particle blending further evidencing applicability for monitoring powder-blending processes. © 2004 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 93:635,645, 2004 [source]


    Beyond the "Model Minority" Stereotype: Trends in Health Risk Behaviors Among Asian/Pacific Islander High School Students

    JOURNAL OF SCHOOL HEALTH, Issue 8 2009
    Sung-Jae Lee PhD
    ABSTRACT BACKGROUND: Asian/Pacific Islander (API) students have been stereotyped as the "model minority." The objective of this study was to examine the trends in health risk behaviors among API students who participated in the San Diego City Schools Youth Risk Behavior Survey (YRBS) between 1993 and 2005. METHODS: High school students from the San Diego City School District completed the self-administered YRBS between 1993 and 2005. Among sexually active students, logistic regression for survey data was used to examine trends in health risk behaviors. RESULTS: From 1993 to 2005, condom use at last sexual intercourse was consistently lower among API students than their cross-ethnic peers. We observed a significant increasing trend in lifetime smoking, drinking, and marijuana use. Parental communications regarding human immunodeficiency virus (HIV) or acquired immunodeficiency syndrome (AIDS) were significantly less frequent and decreased over time. CONCLUSIONS: Our findings challenge the notion of API youth being the "model minority." API students face unique challenges, including barriers to good communication about sex and lower rates of condom use. School-based prevention programs are needed for API students, including a focus on HIV communication with parents. [source]


    How do APIs evolve?

    JOURNAL OF SOFTWARE MAINTENANCE AND EVOLUTION: RESEARCH AND PRACTICE, Issue 2 2006
    A story of refactoring
    Abstract Frameworks and libraries change their APIs. Migrating an application to the new API is tedious and disrupts the development process. Although some tools and ideas have been proposed to solve the evolution of APIs, most updates are done manually. To better understand the requirements for migration tools, we studied the API changes of four frameworks and one library. We discovered that the changes that break existing applications are not random, but tend to fall into particular categories. Over 80% of these changes are refactorings. This suggests that refactoring-based migration tools should be used to update applications. Copyright © 2006 John Wiley & Sons, Ltd. [source]


    Analytical strategies for identifying drug metabolites

    MASS SPECTROMETRY REVIEWS, Issue 3 2007
    Chandra Prakash
    Abstract With the dramatic increase in the number of new chemical entities (NCEs) arising from combinatorial chemistry and modern high-throughput bioassays, novel bioanalytical techniques are required for the rapid determination of the metabolic stability and metabolites of these NCEs. Knowledge of the metabolic site(s) of the NCEs in early drug discovery is essential for selecting compounds with favorable pharmacokinetic credentials and aiding medicinal chemists in modifying metabolic "soft spots". In development, elucidation of biotransformation pathways of a drug candidate by identifying its circulatory and excretory metabolites is vitally important to understand its physiological effects. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) have played an invaluable role in the structural characterization and quantification of drug metabolites. Indeed, liquid chromatography (LC) coupled with atmospheric pressure ionization (API) MS has now become the most powerful tool for the rapid detection, structure elucidation, and quantification of drug-derived material within various biological fluids. Often, however, MS alone is insufficient to identify the exact position of oxidation, to differentiate isomers, or to provide the precise structure of unusual and/or unstable metabolites. In addition, an excess of endogenous material in biological samples often suppress the ionization of drug-related material complicating metabolite identification by MS. In these cases, multiple analytical and wet chemistry techniques, such as LC-NMR, enzymatic hydrolysis, chemical derivatization, and hydrogen/deuterium-exchange (H/D-exchange) combined with MS are used to characterize the novel and isomeric metabolites of drug candidates. This review describes sample preparation and introduction strategies to minimize ion suppression by biological matrices for metabolite identification studies, the application of various LC-tandem MS (LC-MS/MS) techniques for the rapid quantification and identification of drug metabolites, and future trends in this field. © 2007 Wiley Periodicals, Inc., Mass Spec Rev [source]


    Oral yeast carriage in patients with advanced cancer

    MOLECULAR ORAL MICROBIOLOGY, Issue 2 2002
    A. N. Davies
    The aim of this study was to investigate oral yeast carriage amongst patients with advanced cancer. Oral rinse samples were obtained from 120 subjects. Yeasts were isolated using Sabouraud's dextrose agar and CHROMagarÔ Candida, and were identified using a combination of the API 20 C AUX yeast identification system, species-specific PCR and 26S rDNA gene sequencing. Oral yeast carriage was present in 66% of subjects. The frequency of isolation of individual species was: Candida albicans, 46%; Candida glabrata, 18%; Candida dubliniensis, 5%; others, <,5%. The increasing isolation of non- Candida albicans species is clinically important, since these species are often more resistant to antifungal drugs. Oral yeast carriage was associated with denture wearing (P = 0.006), and low stimulated whole salivary flow rate (P = 0.009). Identification of these risk factors offers new strategies for the prevention of oral candidosis in this group of patients. [source]


    Artificial intelligence advancements applied in off-the-shelf controllers

    PROCESS SAFETY PROGRESS, Issue 2 2002
    Edward M. Marszal P.E.
    Since the earliest process units were built, CPI engineers have employed artificial intelligence to prevent losses. The expanding use of computer-based systems for process control has allowed the amount of intelligence applied in these expert systems to drastically increase. Standard methods for performing Expert System tasks are being formalized by numerous researchers in industry and academia. Work products from these groups include designs that present process hazards knowledge in a structured, hierarchical, and modular manner. Advancements in programmable logic controller (PLC) technology have created systems with substantial computing power that are robust and fault tolerant enough to be used in safety critical applications. In addition, IEC 1131-3 standardized the programming languages available in virtually every new controller. The function block language defined in IEC 1131-3 is particularly well suited to performing modular tasks, which makes it an ideal platform for representing knowledge. This paper begins by describing some of the advancements in knowledge-based systems for loss prevention applications. It then explores how standard IEC 1131-3 programming techniques can be used to build function blocks that represent knowledge of the hazards posed by equipment items. The paper goes on to develop a sample function block that represents the hazards of a pressure vessel, using knowledge developed in the API 14-C standard. [source]


    Separation of a BMS drug candidate and acyl glucuronide from seven glucuronide positional isomers in rat plasma via high-performance liquid chromatography with tandem mass spectrometric detection

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 11 2006
    Y.-J. Xue
    A high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the determination of a BMS drug candidate and its acyl glucuronide (1- O - , glucuronide) in rat plasma. A 50-µL aliquot of each plasma sample was fortified with acetonitrile containing the internal standard to precipitate proteins and extract the analytes of interest. After mixing and centrifugation, the supernatant from each sample was transferred to a 96-well plate and injected into an LC/MS/MS system. Chromatographic separation was achieved isocratically on a Phenomenex Luna C18, 3,mm,×,150,mm, 3,µm column. The mobile phase contained 0.075% formic acid in 70:30 (v/v) acetonitrile/water. Under the optimized chromatographic conditions, the BMS drug candidate and its acyl glucuronide were separated from its seven glucuronide positional isomers within 10,min. Resolution of the parent from all glucuronides and acyl glucuronide from its positional isomers was critical to avoid their interference with quantitation of parent or acyl glucuronide. Detection was by positive ion electrospray MS/MS on a Sciex API 4000. The standard curve, which ranged from 5 to 5000,ng/mL, was fitted to a 1/x2 weighted quadratic regression model for both the BMS drug candidate and its acyl glucuronide. Whole blood and plasma stability experiments were conducted to establish the sample collection, storage, and processing conditions. The validation results demonstrated that this method was rugged and repeatable. The same methodology has also been used in mouse and human plasma for the determination of the BMS drug candidate and its acyl glucuronide. Copyright © 2006 John Wiley & Sons, Ltd. [source]


    Differentiation of isomeric flavone/isoflavone aglycones by MS2 ion trap mass spectrometry and a double neutral loss of CO

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 17 2003
    Fabian Kuhn
    The fragmentation behaviour of seven pairs of isomeric flavone/isoflavone aglycones (solely hydroxylated and/or methoxylated) was studied using ion trap mass spectrometry with atmospheric pressure ionisation (API, both electrospray and APCI) in the positive and negative ion modes. A major difference was found in the neutral loss of 56,u, which was a common feature of all isoflavones in API(+). It was identified as a double loss of CO by accurate mass tandem mass spectrometric (MS/MS) measurements using a hybrid quadrupole time-of-flight (Q-TOF) instrument. Fragmentation of daidzein with 13C-isotope labelling of the carbon C2 showed that this double loss occurred from the central ring of the molecule. A mechanism for this selective fragmentation is given. Further isoflavone-specific fragmentations were used to develop a guideline for the identification of isoflavone structures. A software-based neutral loss scan of 56,u in the API(+)-MS2 mode was applied to extracts of leaves of Lupinusalbus and to soy flour. The structure elucidation guideline allowed identification of hydroxy and/or methoxy isoflavones. Structures could be confirmed for those available as reference compounds. Copyright © 2003 John Wiley & Sons, Ltd. [source]


    The use of particle beam mass spectrometry for the measurement of impurities in a nabumetone drug substance, not easily amenable to atmospheric pressure ionisation techniques

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 4 2001
    Jean-Claude Wolff
    Liquid chromatography/particle beam mass spectrometry (LC/PB-MS) was used for the structural elucidation of some impurities in nabumetone as this compound poorly ionises by atmospheric pressure ionisation (API) techniques. PB-MS was optimised for nabumetone and a sensitivity study was carried out. To obtain full scan electron ionisation spectra a minimum of 100,ng of compound on column was needed. By using 20,mg/mL solutions of nabumetone, impurities at levels of about 250,ppm mass fraction relative to nabumetone could be detected. Results were compared with LC/API-MS and previous GC/MS. Copyright © 2001 John Wiley & Sons, Ltd. [source]


    Off label and unlicensed prescribing in a specialist oncology center in Australia

    ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY, Issue 4 2009
    James D MELLOR
    Abstract Aim: Oncology is an area with a high prevalence of off-label and unlicensed prescribing. A previous audit conducted in 2001 at the Peter MacCallum Cancer Centre, a specialist oncology hospital, showed that 22% of all prescriptions were either off-label or unlicensed. This study aimed to determine if the rates of off-label and unlicensed prescribing in oncology had changed between 2001 and 2008. Methods: All prescriptions at the Peter MacCallum Cancer Centre were reviewed on a single day in March 2008. Each prescription was classified as licensed, off-label or unlicensed by comparing to the Approved Product Information (API). Results: The medications of 132 patients were assessed. Among the 1094 prescriptions, 382 (35%) were off-label and 44 (4%) were unlicensed. 112 (85%) patients received at least one off-label or unlicensed drug. Conclusion: The results of the audit suggest that the level of off-label prescribing increased by 17% since 2001. This study confirmed the extensive off-label and unlicensed drug usage as suggested by the literature and demonstrated a higher rate of off-label prescribing than the previous audit in 2001. The results demonstrate that 85% of all cancer patients in the study were prescribed at least one drug that had not been fully tested by the regulatory approval process. [source]


    Service capability interaction management in IMS using the Lucent Service BrokerÔ product

    BELL LABS TECHNICAL JOURNAL, Issue 4 2006
    Kristin F. Kocan
    The 3rd Generation Partnership Project/3rd Generation Partnership Project 2 (3GPP/3GPP2) designates the service capability interaction manager (SCIM) as a functional component in the IP Multimedia Subsystem (IMS). This paper focuses on how the Lucent Service BrokerÔ product has been designed and how it would be deployed to perform as a SCIM in an IMS network. We discuss the challenges in managing service capability interaction and providing blended services that are encountered in IMS and show how Lucent Service Broker flexibility is needed to accommodate the breadth of challenges. We describe how the internal structure of the Lucent Service Broker and its application programming interface (API) offer this flexibility while minimizing the effort involved in adding new SCIM logic. We also demonstrate how the Lucent Service Broker may be used to marshal IMS application resources on behalf of revenue-generating applications in various usage scenarios. © 2006 Lucent Technologies Inc. [source]