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Key Observations (key + observation)
Selected AbstractsIDENTIFICATION OF IMPORTANT PRODUCTION VARIABLES AFFECTING HARD PRETZEL QUALITYJOURNAL OF FOOD QUALITY, Issue 3 2005N. YAO ABSTRACT The objective of this study was to determine the importance of raw material and processing variables that influence pretzel quality by utilizing a screening experiment design. Eleven variables were selected based on preliminary experiments, and a two-level-11-factor (211) fractional factorial experimental design was used to screen the variables. Several responses were measured for dough before and after extrusion, for half-baked and fully baked pretzels. These responses are important indicators of consistency and quality during pretzel processing. Results indicated that flour protein content, the amount of water added to make dough and dough mixing time were important variables influencing dough behavior. Caustic concentration affected brightness of half-baked pretzels but did not influence the color of the final product. Baking time was the most important factor for both half-baked product and final product qualities. The hardness of fully baked pretzels was influenced by baking time, temperature in baking oven zone 1, drying time and drying temperature. The color of final products was significantly influenced by baking time, while both baking time and drying temperature affected the moisture content of the final product. A key observation was that none of the raw material or dough processing parameters, within the range tested, influenced final pretzel quality as defined by pretzel moisture content, hardness or color. [source] A personal account of the role of peptide research in drug discovery: the case of hepatitis C,JOURNAL OF PEPTIDE SCIENCE, Issue 1 2001Antonello Pessi Abstract Although peptides themselves are not usually the end products of a drug discovery effort, peptide research often plays a key role in many aspects of this process. This will be illustrated by reviewing the experience of peptide research carried out at IRBM in the course of our study of hepatitis C virus (HCV). The target of our work is the NS3/4A protease, which is essential for maturation of the viral polyprotein. After a thorough examination of its substrate specificity we fine-tuned several substrate-derived peptides for enzymology studies, high-throughput screening and as fluorescent probes for secondary binding assays. In the course of these studies we made the key observation: that the protease is inhibited by its own cleavage products. Single analog and combinatorial optimization then derived potent peptide inhibitors. The crucial role of the NS4A cofactor was also addressed. NS4A is a small transmembrane protein, whose central domain is the minimal region sufficient for enzyme activation. Structural studies were performed with a peptide corresponding to the minimal activation domain, with a series of product inhibitors and with both. We found that NS3/4A is an induced fit enzyme, requiring both the cofactor and the substrate to acquire its bioactive conformation; this explained some puzzling results of ,serine-trap' type inhibitors. A more complete study on NS3 activation, however, requires the availability of the full-length NS4A protein. This was prepared by native chemical ligation, after sequence engineering to enhance its solubility; structural studies are in progress. Current work is focused on the P, region of the substrate, which, at variance with the P region, is not used for ground state binding to the enzyme and might give rise to inhibitors showing novel interactions with the enzyme. Copyright © 2001 European Peptide Society and John Wiley & Sons, Ltd. [source] Bayesian clustering and product partition modelsJOURNAL OF THE ROYAL STATISTICAL SOCIETY: SERIES B (STATISTICAL METHODOLOGY), Issue 2 2003Fernando A. Quintana Summary. We present a decision theoretic formulation of product partition models (PPMs) that allows a formal treatment of different decision problems such as estimation or hypothesis testing and clustering methods simultaneously. A key observation in our construction is the fact that PPMs can be formulated in the context of model selection. The underlying partition structure in these models is closely related to that arising in connection with Dirichlet processes. This allows a straightforward adaptation of some computational strategies,originally devised for nonparametric Bayesian problems,to our framework. The resulting algorithms are more flexible than other competing alternatives that are used for problems involving PPMs. We propose an algorithm that yields Bayes estimates of the quantities of interest and the groups of experimental units. We explore the application of our methods to the detection of outliers in normal and Student t regression models, with clustering structure equivalent to that induced by a Dirichlet process prior. We also discuss the sensitivity of the results considering different prior distributions for the partitions. [source] White pines, Ribes, and blister rust: a review and synthesisFOREST PATHOLOGY, Issue 3-4 2010Brian W. Geils Summary For over a century, white pine blister rust (Cronartium ribicola) has linked white pines (Strobus) with currants and gooseberries (Ribes) in a complex and serious disease epidemic in Asia, Europe, and North America. Because of ongoing changes in climate, societal demands for forests and their amenities, and scientific advances in genetics and proteomics, our current understanding and management of the white pine blister rust pathosystem has become outdated. Here, we present a review and synthesis of international scope on the biology and management of blister rust, white pines, Ribes, and other hosts. In this article, we provide a geographical and historical background, describe the taxonomy and life cycle of the rust, discuss pathology and ecology, and introduce a series of invited papers. These review articles summarize the literature on white pines, Ribes, and blister rust with respect to their status, threats, and management through genetics and silviculture. Although the principal focus is on North America, the different epidemics in Europe and Asia are also described. In the final article, we discuss several of the key observations and conclusions from the preceding review articles and identify prudent actions for research and management of white pine blister rust. [source] Controls of mantle plumes and lithospheric folding on modes of intraplate continental tectonics: differences and similaritiesGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 3 2009Evgueni Burov SUMMARY Mantle plume activity and lithospheric folding by far-field stresses exerted from plate boundaries are two important end-members as mechanisms for continental intraplate deformation. The topographic expression of mantle plume impingement on continental lithosphere and lithospheric folding has some striking similarities. Observations from a number of areas in Europe's intraplate lithosphere demonstrate that these mechanisms commonly interact in space and time. We present the results of thermomechanical modelling addressing the role of factors such as the presence of a hot upper mantle, the spatial dimensions of the plume and the time constants involved in the temporal succession of plume activity and lithospheric folding by stress accumulation in intraplate continental lithosphere. The results demonstrate that both the processes, plume,lithosphere interactions and folding may interact resulting either in strong amplification, attenuation or modification of their surface expression. These inferences are compatible with a number of key observations on the nature and the temporal succession of topography evolution in the Alpine foreland, the Pannonian Basin, the Scandinavian continental margin and the Iberian Peninsula. [source] The Human Ageing Genomic Resources: online databases and tools for biogerontologistsAGING CELL, Issue 1 2009Joćo Pedro De Magalhćes Summary Aging is a complex, challenging phenomenon that requires multiple, interdisciplinary approaches to unravel its puzzles. To assist basic research on aging, we developed the Human Ageing Genomic Resources (HAGR). This work provides an overview of the databases and tools in HAGR and describes how the gerontology research community can employ them. Several recent changes and improvements to HAGR are also presented. The two centrepieces in HAGR are GenAge and AnAge. GenAge is a gene database featuring genes associated with aging and longevity in model organisms, a curated database of genes potentially associated with human aging, and a list of genes tested for their association with human longevity. A myriad of biological data and information is included for hundreds of genes, making GenAge a reference for research that reflects our current understanding of the genetic basis of aging. GenAge can also serve as a platform for the systems biology of aging, and tools for the visualization of protein,protein interactions are also included. AnAge is a database of aging in animals, featuring over 4000 species, primarily assembled as a resource for comparative and evolutionary studies of aging. Longevity records, developmental and reproductive traits, taxonomic information, basic metabolic characteristics, and key observations related to aging are included in AnAge. Software is also available to aid researchers in the form of Perl modules to automate numerous tasks and as an SPSS script to analyse demographic mortality data. The HAGR are available online at http://genomics.senescence.info. [source] Managing radical innovation: an overview of emergent strategy issuesTHE JOURNAL OF PRODUCT INNOVATION MANAGEMENT, Issue 6 2002Christopher M. McDermott Despite differences in definitions, researchers understand that radical innovation within an organization is very different from incremental innovation [13,17,21] and that it is critical to the long-term success of firms. Unfortunately, research has also shown that it is often difficult to get support for radical projects in large firms [14], where internal cultures and pressures often push efforts toward more low risk, immediate reward, incremental projects. Interestingly, we know considerably less about the effective management of the product development process in the radical than in an incremental context. The purpose of this study is to explore the process of radical new product development from a strategic perspective, and to outline key observations and challenges that managers face as they move these projects to market. The findings presented here represent the results of a longitudinal (since 1995), multidisciplinary study of radical innovation projects. A multiple case study design was used to explore the similarities and differences in management practices applied to twelve radical innovation projects in ten large, established North American firms. The findings are grouped into three high-level strategic themes. The first theme, market scope, discusses the challenges associated with the pursuit of familiar versus unfamiliar markets for radical innovation. The second theme of competency management identifies and discusses strategic challenges that emerge as firms stretch themselves into new and unfamiliar territory. The final theme relates to the people issues that emerge as both individuals and the project teams themselves try to move radical projects forward in organizations that are not necessarily designed to support such uncertainty. A breadth of subtopics emerge within and across this framework relating to such ideas as risk management, product cannibalization, team composition, and the search for a divisional home. Taken together, our observations reinforce the emerging literature that shows that project teams engaging in radical innovation encounter a much different set of challenges than those typically faced by NPD teams engaged in incremental innovation. [source] | |||||||||||||