Individual Pairs (individual + pair)

Distribution by Scientific Domains


Selected Abstracts


Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)- L -phenylalaninamide complex

JOURNAL OF SEPARATION SCIENCE, JSS, Issue 18 2009
Li Qi
Abstract A novel method of chiral ligand-exchange CE was developed with L -amino acylamides as a chiral ligand and zinc(II) as a central ion. It has been demonstrated that these chiral complexes, such as Zn(II)- L -alaninamide, Zn(II)- L -prolinamide, and Zn(II)- L -phenylalaninamide, are suitable for use as chiral selectors for the enantioseparation of either individual pair of or mixed dansyl amino acids. The optimal separation running buffer consisted of 5 mM ammonium acetate, 100 mM boric acid, 4 mM ZnSO4·7 H2O, and 8 mM L -amino acylamides at pH 8.2. The experiments showed that apart from the effect of the concentration of the complexes on the resolution and the migration time, the buffer pH also had a sharp influence on resolution. The employed chiral ligands exhibited different enantioselectivities and enantiomer migration orders. D -Amino acids migrate faster than L -amino acids when Zn(II)- L -alaninamide and Zn(II)- L -phenylalaninamide are used as chiral selectors, but it was observed that the migration order is reversed when Zn(II)- L -prolinamide is used as the chiral selector. Furthermore, the amount of dansylated amino acids is found to be highly dependent on the labeling temperature. [source]


Chiral CE of aromatic amino acids by ligand-exchange with zinc(II),L -lysine complex

ELECTROPHORESIS, Issue 15 2007
Li Qi
Abstract A novel method of chiral ligand-exchange CE was developed with either L - or D -lysine (Lys) as a chiral ligand and zinc(II) as a central ion. This type of chiral complexes was explored for the first time to efficiently separate either individual pairs of or mixed aromatic amino acid enantiomers. Using a running buffer of 5,mM ammonium acetate, 100,mM boric acid, 3,mM ZnSO4·7H2O and 6,mM L -Lys at pH,7.6, unlabeled D,L -tryptophan, D,L -phenylalanine, and D,L -tyrosine were well separated, giving a chiral resolution of up to 7.09. The best separation was obtained at a Lys-to-zinc ratio of 2:1, zinc concentration of 2,4,mM and running buffer pH,7.6. The buffer pH was determined to have a strong influence on resolution, while buffer composition and concentration impacted on both the resolution and peak shape. Boric acid with some ammonium acetate was an adoptable buffer system, and some additives like ethylene diamine tetraacetic acid capable of destroying the complex should be avoided. Fine-tuning of the chiral resolution and elution order was achieved by regulating the ratio of L -Lys to D -Lys; i.e. the resolution increased from zero to its highest value as the ratio ascended from 1:0 to 1:infinitive, and L -isomers eluted before or after D -isomers in excessive D - or L -Lys, respectively. [source]


Costs and benefits of breeding in human-altered landscapes for the Eagle Owl Bubo bubo

IBIS, Issue 4 2002
Luigi Marchesi
We studied a population of 23,25 Eagle Owl Bubo bubo pairs between 1994 and 2000 in a 1330-km2 study plot in the central-eastern Italian Alps. Compared to random sites, territories were located at lower elevation and closer to intensively cultivated-urbanized valley floors. Early laying was associated with low elevation and negatively affected productivity. Diet was dominated by rats, hedgehogs and dormice (n = 978 prey items), all of them typical of low-elevation habitats. Higher productivity was associated with a higher proportion of rats in the diet of individual pairs. Low availability of rats resulted in a more diverse diet, in turn associated with low productivity. Territories were occupied every year in a non-random fashion, and those most occupied were characterized by higher productivity and higher occurrence of the favoured prey types in the diet, suggesting they were of superior quality. Eagle Owls also paid a cost associated with nesting near human-altered habitats: the main cause of mortality reported to local authorities was electrocution. This is an increasing cause of death for many European populations and may be a cause for conservation concern. Human persecution is also an important cause of mortality in some parts of the European range. Apart from such costs, the study population appeared to have adapted well to the proximity of humans: estimates of density and productivity were comparable to those recorded elsewhere in Europe. The pattern found in our population also held at higher spatial scales: data from 17 European populations showed density to be highest in low-elevation, human-altered landscapes. [source]


Estimation of pairwise relatedness between individuals and characterization of isolation-by-distance processes using dominant genetic markers

MOLECULAR ECOLOGY, Issue 6 2003
Olivier J. Hardy
Abstract A new estimator of the pairwise relatedness coefficient between individuals adapted to dominant genetic markers is developed. This estimator does not assume genotypes to be in Hardy,Weinberg proportions but requires a knowledge of the departure from these proportions (i.e. the inbreeding coefficient). Simulations show that the estimator provides accurate estimates, except for some particular types of individual pairs such as full-sibs, and performs better than a previously developed estimator. When comparing marker-based relatedness estimates with pedigree expectations, a new approach to account for the change of the reference population is developed and shown to perform satisfactorily. Simulations also illustrate that this new relatedness estimator can be used to characterize isolation by distance within populations, leading to essentially unbiased estimates of the neighbourhood size. In this context, the estimator appears fairly robust to moderate errors made on the assumed inbreeding coefficient. The analysis of real data sets suggests that dominant markers (random amplified polymorphic DNA, amplified fragment length polymorphism) may be as valuable as co-dominant markers (microsatellites) in studying microgeographic isolation-by-distance processes. It is argued that the estimators developed should find major applications, notably for conservation biology. [source]