Analysis Device (analysis + device)

Distribution by Scientific Domains

Selected Abstracts

Development of Air Micro Bubbles in the Venous Outlet Line: An In Vitro Analysis of Various Air Traps Used for Hemodialysis

Christofer J. Stegmayr
Abstract:, Venous air traps were tested in vitro with respect to presence of micro bubbles. Three types of venous air traps were measured (Bioline, Bioline GmbH, Luckenwalde, Germany; Gambro, Gambro AB, Lund, Sweden; Fresenius M.C., Fresenius Medical Care AG & Co. KGaA, Bad Homburg, Germany). Measurements (n = 10) were taken for each air trap, fluid flow (50,600 mL/min), and fluid level (high/low). A 1.5-MHz ultrasound probe was used with an analysis device. The probe was mounted on the outlet line downstream of the venous air trap. A semisynthetic fluid was used to resemble blood viscosity. Occurrences of micro bubbles, without inducing an alarm of the dialysis device, were detected in almost all measurements. The amount of bubbles increased with increasing flow. There were more bubbles with low fluid level compared with high level. The Bioline tubing released the least bubbles in high fluid level. At low level, the Gambro tubing showed the least bubbles at flows 50,400 mL/min, and the Fresenius M.C. tubing showed the least bubbles at flows 400,600 mL/min. High fluid level in the air trap reduced generation of micro bubbles compared to low level, as did lower fluid flow versus high flow. The design of the air trap was also of importance. [source]

The use of a spectrophotometric intracutaneous analysis device in the real-time diagnosis of melanoma in the setting of a melanoma screening clinic

M.A. Haniffa
Summary Background, Skin imaging devices to aid melanoma diagnosis have been developed in recent years but few have been assessed clinically. Objectives, To investigate if a spectrophotometric skin imaging device, the SIAscope, could increase a dermatologist's ability to distinguish melanoma from nonmelanoma in a melanoma screening clinic. Methods, Eight hundred and eighty-one pigmented lesions from 860 patients were prospectively assessed clinically and with the aid of the spectrophotometric device by a dermatologist. Assessment before and after spectrophotometric imaging was made and compared with histology, where available, or with the clinical diagnosis of a dermatologist with 20 years of experience. Results, One hundred and seventy-nine biopsies were performed, with 31 melanomas diagnosed. Sensitivity and specificity for melanoma diagnosis before and after spectrophotometry were 94% and 91% vs. 87% and 91%, respectively, with no significant difference in the area under the receiver operating characteristic curves (0ˇ932 and 0ˇ929). Conclusions, Our study provides no evidence for the use of SIAscope by dermatologists to help distinguish melanoma from benign lesions. [source]

Functionalization Strategies for Protease Immobilization on Magnetic Nanoparticles

Dan Li
Abstract A comprehensive study on the general functionalization strategies for magnetic nanoparticles (MNPs) is presented in this work. Using well-established techniques as well as modified protocols, the wide range of functional moieties grafted on ,-Fe2O3 (maghemite) nanosurfaces include those of amine, aldehyde, carboxylic, epoxy, mercapto, and maleimide ends. Among the modified protocols are the one-step water-catalyzed silanization with mercaptopropyltrimethoxysilane, resulting in dense distal thiols, and the direct functionalization with a heterogeneous bifunctional linker N -[p-maleimidophenyl]isocynanate (PMPI). The former results in a protective Stöber type coating while simultaneously reducing the iron oxide core to magnetite (Fe3O4). The conjugation of trypsin, hereby chosen as model biomolecule, onto the differently functionalized MNPs is further demonstrated and assessed based on its activity, kinetics, and thermo-/long-term stability as well as reusability. Besides aqueous stability and ease in recovery by magnetic separation, the immobilized trypsin on MNPs offers superior protease durability and reusability, without compromising the substrate specificity and sequence coverage of free trypsin. The MNP-based proteases can be used as valuable carriers in proteomics and miniaturized total analysis devices. The applicability of the functional surfaces devised in the current study is also relevant for the conjugation of other biomolecules beyond trypsin. [source]

Cover Picture: Multipotent Polymer Coatings Based on Chemical Vapor Deposition Copolymerization (Adv. Mater.

Abstract The cover shows that chemical vapor deposition can be used to prepare copolymer thin films, on varying substrates, that can bind two different ligands with high selectivity. In work reported by Lahann and co-workers on p.,1521, the actual ligand ratios on the surface can be controlled by varying the copolymer composition. This technology may find applications in biomedical devices, high-throughput screening platforms, microfluidic analysis devices, and diagnostic platforms. [source]