Home  About us  Contact
 

Ionization Mass Spectrometric (ionization + mass_spectrometric)

Distribution by Scientific Domains
Chemistry100%

Terms modified by Ionization Mass Spectrometric

  • ionization mass spectrometric analysis
    		•
  • ionization mass spectrometric study
    		•

    Selected Abstracts

    Separation of triacylglycerols in a complex lipidic matrix by using comprehensive two-dimensional liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometric detection

    JOURNAL OF SEPARATION SCIENCE, JSS, Issue 8 2006
    Paola Dugo
    Abstract The present investigation describes the employment of a comprehensive 2-D HPLC system, based on the combination of a silver ion and an RP column, for the characterization of the triacylglycerol (TAG) fraction of a very complex lipidic sample: donkey milk fat. The TAGs were grouped on the resulting bidimensional contour plot according to their double bond numbers (aligned along vertical bands) and according to their partition numbers (aligned along horizontal bands). Peak assignment was supported by using atmospheric pressure chemical ionization mass spectrometric (APCI-MS) detection. The combination of the enhanced resolving power of comprehensive multidimensional LC, the formation of ordered 2-D patterns, and APCI-MS detection proved to be an effective tool for the characterization of the complex matrix, enabling the separation and identification of nearly 60 TAGs. [source]

    Evaluation of tramadol and its main metabolites in horse plasma by high-performance liquid chromatography/fluorescence and liquid chromatography/electrospray ionization tandem mass spectrometry techniques

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 2 2009
    Marinella De Leo
    Tramadol is a centrally acting analgesic drug that has been used clinically for the last two decades to treat pain in humans. The clinical response of tramadol is strictly correlated to its metabolism, because of the different analgesic activity of its metabolites. O -Desmethyltramadol (M1), its major active metabolite, is 200 times more potent at the µ -receptor than the parent drug. In recent years tramadol has been widely introduced in veterinary medicine but its use has been questioned in some species. The aim of the present study was to develop a new sensible method to detect the whole metabolic profile of the drug in horses, through plasma analyses by high-performance liquid chromatography (HPLC) coupled with fluorimetric (FL) and photodiode array electrospray ionization mass spectrometric (PDA-ESI-MS) detection, after its sustained release by oral administration (5,mg/kg). In HPLC/FL experiments the comparison of the horse plasma chromatogram profile with that of a standard mixture suggested the identification of the major peaks as tramadol and its metabolites M1 and N,O -desmethyltramadol (M5). LC/PDA-ESI-MS/MS analysis confirmed the results obtained by HPLC/FL and also provided the identification of two more metabolites, N -desmethyltramadol (M2), and N,N -didesmethyltramadol (M3). Another metabolite, M6, was also detected and identified. The present findings demonstrate the usefulness and the advantage of LC/ESI-MS/MS techniques in a search for tramadol metabolites in horse plasma samples. Copyright © 2008 John Wiley & Sons, Ltd. [source]

    Electrospray mass spectrometry studies of macromolecules containing 1,3-oxazolidine moieties

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 22 2004
    J. Ma, ska-Solich
    The polycondensation of terephthalaldehyde with 2-amino-2-methyl-1,3-propanediol using acidic catalysts leads to the formation of linear polymers and macrocyclic compounds with 2-(1,,4,-phenylene)-5-methyl-1-aza-3,7-dioxabicyclo[3.3.0]octane as the repeating unit in all compounds. The structure of the polymer was determined by 1H- and 13C-NMR spectroscopy, and electrospray ionization mass spectrometric (ESI-MS) analysis. ESI-MS measurements were used to study the details of the polymer structure and support the equimolar alternating nature of bicyclo- O,N -acetals of terephthaldehyde in these macromolecules. Copyright © 2004 John Wiley & Sons, Ltd. [source]

    Quantitative characterization of differential ion suppression on liquid chromatography/atmospheric pressure ionization mass spectrometric bioanalytical methods

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 3 2003
    Michael J. Avery
    Ion suppression is a known phenomenon in atmospheric pressure ionization mass spectrometry. These suppression effects have been shown to adversely affect the accuracy and precision of quantitative bioanalytical methods. This paper presents a simple procedure for determining the impact of differential ion suppression on bioanalytical methods that utilize atmospheric pressure ionization mass spectrometric (APIMS) detection. This procedure was applied to the assessment of two potential internal standards, and to determine selectivity issues for another analyte which was to be measured in multiple species. Copyright © 2002 John Wiley & Sons, Ltd. [source]