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IL-4 Treatment (il-4 + treatment)

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Medical Sciences	100%

Selected Abstracts

Low expression of the interleukin (IL)-4 receptor alpha chain and reduced signalling via the IL-4 receptor complex in human neonatal B cells

IMMUNOLOGY, Issue 1 2006
Cuixia Tian
Summary Diminished neonatal antibody responses following infection or immunization may stem in part from intrinsic characteristics of neonatal B cells. In this study, we used B-cell subset sorting combined with gene expression assays to investigate major differences in the expression of host genes in neonatal and adult naïve B cells. We discovered significantly reduced expression of the interleukin (IL)-4 receptor alpha chain and reduced IL-4-induced signalling in neonatal B cells. Neonatal naïve B cells were susceptible to more rapid and more profound levels of apoptosis when cultured in vitro. They also exhibited a limited response to IL-4 treatment compared with adult cells. The expression level of the IL-13 receptor alpha 1 chain, a key component of the IL-13 receptor/IL-4 type II receptor, and the response to IL-13 treatment for protection against apoptosis in neonatal B cells were similar to those of the adult B cells. These studies suggest a possible mechanism underlying the limited magnitude and durability of neonatal antibody responses. [source]

Role of Interleukins and Transforming Growth Factor-, in Chronic Rhinosinusitis and Nasal Polyposis

THE LARYNGOSCOPE, Issue 4 2005
Dewayne T. Bradley MD
Abstract Objectives: To determine the role of interleukin (IL)-4, IL-4 receptor (R), IL-6, IL-8, IL-11, and transforming growth factor (TGF)-, in chronic rhinosinusitis (CRS) and chronic rhinosinusitis with nasal polyposis (CRS/NP). Methods: Sinus tissue from patients undergoing endoscopic sinus surgery for CRS and CRS/NP was collected. Sinus tissue was then analyzed using reverse-transcription polymerase chain reaction (RT-PCR) to detect transcription of IL-4R, IL-6, IL-8, and IL-11. Sinus tissue samples were also cultured in vitro, treated with IL-4 for 24 hours, and real-time PCR was used to quantify the transcription of TGF-,. Results: Twenty patients were evaluated, 9 with CRS/NP and 11 with CRS alone. The mean age was 43 (20,74) years, with 13 females and 7 males. IL-4R, IL-6, IL-8, and IL-11 were identified by RT-PCR in all 20 patients. The transcription of TGF-, was found to be 3.2 times greater in patients with CRS/NP than in patients with CRS alone (P = .047). Conclusion: IL-6, IL-8, and IL-11 are nonspecific markers of sinus inflammation being transcribed in patients with CRS and patients with CRS/NP. However, patients with CRS/NP demonstrate increased transcription of TGF-, in response to IL-4 treatment, suggesting an IL-4 mediated mechanism for stromal proliferation in the formation of nasal polyposis. [source]

Interleukin-4 increases murine airway response to kinins, via up-regulation of bradykinin B1 -receptors and altered signalling along mitogen-activated protein kinase pathways

CLINICAL & EXPERIMENTAL ALLERGY, Issue 8 2004
M. Bryborn
Summary Background IL-4 is believed to play a role in asthma and chronic obstructive pulmonary disease through promotion of eosinophilic inflammation and mucus hypersecretion. Whether IL-4 can induce a direct effect on airway smooth muscle remains unknown. Objective To investigate the effect of IL-4 on airway smooth muscle, focusing on the contractile response to des-Arg9 -bradykinin and bradykinin. Methods Tracheal segments from murine airways were cultured for 1,8 days in the absence and presence of IL-4. The smooth muscle response induced by des-Arg9 -bradykinin and bradykinin was investigated in myographs. Expression levels for the IL-4-, bradykinin B1 - and B2 -receptors were characterized using RT-PCR. Specific inhibitors were used to study signal changes along the IL-4 receptor- (IL-4R-) coupled mitogen-activated protein (MAP) kinase (MAPK) pathways. Results IL-4 treatment increased the contractile response to des-Arg9 -bradykinin and bradykinin in a concentration- and time-dependent manner. Dexamethasone and the transcriptional inhibitor actinomycin D blocked this effect. c-Jun N-terminal kinase inhibitor SP600125 also blocked the effect of both des-Arg9 -bradykinin and bradykinin, whereas p38 inhibitor SB203580 blocked only the former and the MAPKK inhibitor PD098059, only the latter agonist responses. IL-4 treatment increased the mRNA levels representing bradykinin B1 - but not B2 -receptors. Levels of IL-4R were not altered during culture. Conclusion Long-term exposure to IL-4 increases the contractile response induced by des-Arg9 -bradykinin and bradykinin in cultured murine airways. This effect appears to be mediated via an up-regulation of B1 -receptors and altered signalling along the MAPK pathways. [source]