Hydroxyphenylacetic Acid (hydroxyphenylacetic + acid)

Distribution by Scientific Domains

Selected Abstracts

Isolation and characterization of a novel Bacillus sp., strain YAS1, capable of transforming tyrosol under hypersaline conditions

Slim Abdelkafi
Abstract A moderately halotolerant, Gram-positive, aerobic, motile, spore-forming bacterium, designated as strain YAS1, was isolated from an olive-brine fermentation rich in aromatic compounds, after enrichment on tyrosol. Strain YAS1 grew between 25 and 45 C and optimally at 37 C. It grew in the presence of 0,15% (v/w) NaCl, with an optimum of 3,6% (v/w) NaCl. The DNA G + C content was found to be 49.9 mol%. Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Bacillus. The newly isolated strain YAS1 represents the first moderately halotolerant bacterium transforming tyrosol to p -hydroxyphenylacetic acid (PHPA) in the presence of yeast extract. [source]

Modeling the solubility of pharmaceuticals in pure solvents and solvent mixtures for drug process design

Feelly Ruether
Abstract The knowledge of the solubility of pharmaceuticals in pure solvents and solvent mixtures is crucial for designing the crystallization process of drug substances. The first step in finding optimal crystallization conditions is usually a solvent screening. Since experiments are very time consuming, a model which allows for solubility predictions in pure solvents and solvent mixtures based only on a small amount of experimental data is required. In this work, we investigated the applicability of the thermodynamic model perturbed-chain statistical associating fluid theory (PC-SAFT) to correlate and to predict the solubility of exemplary five typical drug substances and intermediates (paracetamol, ibuprofen, sulfadiazine, p -hydroxyphenylacetic acid, and p -aminophenylacetic acid) in pure solvents and solvent mixtures. 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 98:4205,4215, 2009 [source]

Antibacterial substances of low molecular weight isolated from the blowfly, Lucilia sericata

Abstract Low molecular weight compounds were isolated by high-performance liquid chromatography from the maggot or haemolymph extracts of Lucilia sericata (Meigen) (Diptera: Calliphoridae). Using gas chromatography-mass spectrometry analysis, three compounds were obtained: p -hydroxybenzoic acid (molecular weight 138 Da), p -hydroxyphenylacetic acid (molecular weight 152 Da) and octahydro-dipyrrolo[1,2-a;1,,2,-d] pyrazine-5,10-dione (molecular weight 194 Da), also known as the cyclic dimer of proline (or proline diketopiperazine or cyclo[Pro,Pro]). All three molecules revealed antibacterial activity when tested against Micrococcus luteus and/or Pseudomonas aeruginosa, and the effect was even more pronounced when these molecules were tested in combination and caused lysis of these bacteria. [source]

Competitive Enzymatic Fluorescence Immunoassay for Human IgG by Using a Temperature-Sensitive Phase Separating Polymer with Regulated Phase Transition Temperature

Peng LIN
Abstract A new enzymatic fluorescence immunoassay for human IgG was developed using a temperature-sensitive polymer, poly(N -isopropylacrylamide-co-acrylamide) [P(NIP-AA)], as a carrier. The lower critical solution temperature of the P(NIP-AA) containing molar fraction of 8% for AA was 37 C. In a competitive immunoassay, immobilized IgG and the standard IgG (or sample) competed for binding to a horseradish peroxidase labeled antibody at 33 C in homogeneous format. After changing the temperature to separate the polymer-immune complex, the complex precipitate was re-dissolved and determined by coupling with the fluorescence reaction of hydrogen peroxide and p -hydroxyphenylacetic acid. The calibration graph for human IgG was linear over the range of 100,1000 ng/mL with a detection limit of 2.0 ng/mL. The method is rapid, sensitive and simple. The immune reaction efficiency was improved. In addition, the sensitivity of this method was close to that using traditional microtitration plates as carriers. However, the assay was much faster (the assay time decreased from 100,120 to 30 min). The method has been applied to the determination of the human IgG levels in human sera with satisfactory results. [source]