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Hydrophobic Tail (hydrophobic + tail)
Selected AbstractsHighly sensitive and simple fluorescence staining of proteins in sodium dodecyl sulfate-polyacrylamide-based gels by using hydrophobic tail-mediated enhancement of fluorescein luminescenceELECTROPHORESIS, Issue 19-20 2003Chulhun Kang Abstract Fluorescein has an extremely low luminescence intensity in acidic aqueous media. However, when it was bound to proteins, subsequent increase of luminescence intensity took place. Furthermore, when a hydrophobic tail, such as aliphatic hydrocarbons, was introduced to fluorescein, more dramatic increase of luminescence intensity was observed upon binding to proteins. In the present study, by utilizing this luminescence enhancement, three hydrophobic fluorescein dyes (5-dodecanoyl amino fluorescein, 5-hexadecanoyl amino fluorescein, and 5-octadecanoyl amino fluorescein) were examined as noncovalent fluorescent stains of protein bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Effective incorporation of the dyes to proteins in gels was accomplished either simply by adding dyes at the protein fixation step, or by treating gels with a staining solution after the fixation. The sensitivity of this staining method using the fluorescein derivatives was approximately 1 ng/band for most proteins. For some cases, protein bands containing as low as 0.1 ng were successfully visualized. In addition, the detection sensitivity showed much less protein-to-protein variation than silver staining. This new staining method was also successfully applied to two-dimensional electrophoresis of rat brain proteins. Its overall sensitivity was comparable to that of silver staining. [source] Cascade Reactions in an All-Enzyme NanoreactorCHEMISTRY - A EUROPEAN JOURNAL, Issue 46 2009Guillaume Delaittre Dr. Good things come in small packages! Giant amphiphiles, consisting of a polymeric hydrophobic tail and a horseradish peroxidase head, were simultaneously synthesized and self-assembled into vesicles. During the self-assembly process, glucose oxidase was encapsulated to create enzymatic cascade nanoreactors (see picture; ABTS= 2,2,-azido-bis(3-ethylbenzthiazoline-6-sulfonic acid)). [source] Synthesis of polymerizable amphiphiles with critical packing parameters systematically varied,POLYMERS FOR ADVANCED TECHNOLOGIES, Issue 7-8 2006M. H. Li Abstract In this paper the design and synthesis of a group of polymerizable amphiphiles with different ratios of the number of hydrophilic heads and the number of hydrophobic tails are reported. The head/tail number ratio could be viewed as an approximate equivalent of the critical packing parameter. The synthetic procedure was optimized to be extendable. The design is expected to furnish a robust library of polymerizable amphiphiles for formation and immobilization of surfactant phases. Copyright © 2006 John Wiley & Sons, Ltd. [source] Dynamic adsorption of octanols at air/water interfaceTHE CANADIAN JOURNAL OF CHEMICAL ENGINEERING, Issue 4 2010Chi M. Phan Abstract The dynamic adsorptions of 1-, 2- and 3-octanols at air/water interface were monitored using pendant drop method. It has been found that 1-octanol had strongest interfacial effects, yet slowest rate of adsorption. The experimental data were compared with diffusion-controlled and diffusion-kinetic-mixed models. Excepting 3-octanol at 1,mM, all systems required kinetic step. The effective kinetic constant was found varying with the concentration and the carbinol position. The results highlighted the influence of hydrophobic tails on the dynamic adsorption. Les adsorptions dynamiques de 1-, 2- et 3-octanols à l'interface air/eau ont été suivies à l'aide de la méthode de la goutte pendante. On a découvert que 1-octanol présente les effets interfaciaux les plus forts, mais le taux d'adsorption le plus lent. Les données expérimentales ont été comparées à l'aide de modèles par diffusion contrôlée et à mélange diffusion-cinétique. À l'exception de 3-octanol à 1,mM, tous les systèmes ont nécessité une étape cinétique. On a trouvé que la constante cinétique efficace varie avec la concentration et la position du carbinol. Les résultats ont mis en évidence l'influence des queues hydrophobes sur l'adsorption dynamique. [source] | ||||||||||