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Hybrid Device (hybrid + device)

Distribution by Scientific Domains

Polymers and Materials Science	37%

Selected Abstracts

A microfabricated hybrid device for DNA sequencing

ELECTROPHORESIS, Issue 21 2003
Shaorong LiuArticle first published online: 6 NOV 200
Abstract We have created a hybrid device of a microfabricated round-channel twin-T injector incorporated with a separation capillary in order to extend the straight separation distance for high speed and long readlength DNA sequencing. Semicircular grooves on glass wafers are obtained using a photomask with a narrow line-width and a standard isotropic photolithographic etching process. Round channels are made when two etched wafers are face-to-face aligned and bonded. A two-mask fabrication process has been developed to make channels of two different diameters. The twin-T injector is formed by the smaller channels whose diameter matches the bore of the separation capillary, and the "usual" separation channel, now called the connection channel, is formed by the larger ones whose diameter matches the outer diameter of the separation capillary. The separation capillary is inserted through the connection channel all the way to the twin-T injector to allow the capillary bore flush with the twin-T injector channels. The total dead-volume of the connection is estimated to be , 5 pL. To demonstrate the efficiency of this hybrid device, we have performed four-color DNA sequencing on it. Using a 200 ,m twin-T injector coupled with a separation capillary of 20 cm effective separation distance, we have obtained readlengths of 800 plus bases at an accuracy of 98.5% in 56 min, compared to about 650 bases in 100 min on a conventional 40 cm long capillary sequencing machine under similar conditions. At an increased separation field strength and using a diluted sieving matrix, the separation time has been reduced to 20 min with a readlength of 700 bases at 98.5% base-calling accuracy. [source]

Sequencing of real-world samples using a microfabricated hybrid device having unconstrained straight separation channels

ELECTROPHORESIS, Issue 21 2003
Shaorong Liu
Abstract We describe a microfabricated hybrid device that consists of a microfabricated chip containing multiple twin-T injectors attached to an array of capillaries that serve as the separation channels. A new fabrication process was employed to create two differently sized round channels in a chip. Twin-T injectors were formed by the smaller round channels that match the bore of the separation capillaries and separation capillaries were incorporated to the injectors through the larger round channels that match the outer diameter of the capillaries. This allows for a minimum dead volume and provides a robust chip/capillary interface. This hybrid design takes full advantage, such as sample stacking and purification and uniform signal intensity profile, of the unique chip injection scheme for DNA sequencing while employing long straight capillaries for the separations. In essence, the separation channel length is optimized for both speed and resolution since it is unconstrained by chip size. To demonstrate the reliability and practicality of this hybrid device, we sequenced over 1000 real-world samples from Human Chromosome 5 and Ciona intestinalis, prepared at Joint Genome Institute. We achieved average Phred20 read of 675 bases in about 70 min with a success rate of 91%. For the similar type of samples on MegaBACE 1000, the average Phred20 read is about 550,600 bases in 120 min separation time with a success rate of about 80,90%. [source]

Induced SER-Activity in Nanostructured Ag,Silica,Au Supports via Long-Range Plasmon Coupling

ADVANCED FUNCTIONAL MATERIALS, Issue 12 2010
Jiu-Ju Feng
Abstract A novel Ag,silica,Au hybrid device is developed that displays a long-range plasmon transfer of Ag to Au leading to enhanced Raman scattering of molecules largely separated from the optically excited Ag surface. A nanoscopically rough Ag surface is coated by a silica spacer of variable thickness from ,1 to 21,nm and a thin Au film of ,25,nm thickness. The outer Au surface is further functionalized by a self-assembled monolayer (SAM) for electrostatic binding of the heme protein cytochrome c (Cyt c) that serves as a Raman probe and model enzyme. High-quality surface-enhanced resonance Raman (SERR) spectra are obtained with 413,nm excitation, demonstrating that the enhancement results exclusively from excitation of Ag surface plasmons. The enhancement factor is estimated to be 2,×,104,8,×,103 for a separation of Cyt c from the Ag surface by 28,47,nm, corresponding to an attenuation of the enhancement by a factor of only 2,6 compared to Cyt c adsorbed directly on a SAM-coated Ag electrode. Upon immobilization of Cyt c on the functionalized Ag,silica,Au device, the native structure and redox properties are preserved as demonstrated by time- and potential-dependent SERR spectroscopy. [source]

Hybrid Nanoparticle/Organic Devices with Strong Resonant Tunneling Behaviors

ADVANCED FUNCTIONAL MATERIALS, Issue 16 2009
Tianhang Zheng
Abstract A hybrid nanoparticle/organic device consisting of small molecule organic semiconductors and Ag nanoparticles is reported. The single device exhibits unusual properties of organic resonant tunneling diode (ORTD) at low driving voltage region and offers light emission at high voltage. For ORTD, a strong negative differential resistance behavior is demonstrated at room temperature. The current resonance with the peak-to-valley current ratio of over 4.6 and narrow linewidth of only ,1.4,V is achieved. A detailed operating mechanism of the charging and emission modes is proposed, which can be discussed in terms of the strong charge-trapping effect of Ag nanoparticles. The repeatable operations of hybrid device show the mutual influences between two modes and the light emission properties of the ORTD are also discussed. [source]

High-Mobility Nonvolatile Memory Thin-Film Transistors with a Ferroelectric Polymer Interfacing ZnO and Pentacene Channels

ADVANCED MATERIALS, Issue 42 2009
Kwang H. Lee
Nonvolatile memory ferroelectric thin-film transistors (FeTFT) with P(VDF-TrFE) polymer are demonstrated with both n-channel ZnO and p-channel pentacene. A high mobility of ,1,cm2 V,1 s,1 and large memory window of ,20,V are achieved through the organic ferroelectric, inorganic channel hybrid device of ZnO-FeTFT. WRITE/ERASE states are clearly distinguished by ±20,V switching for ZnO- and pentacene-FeTFTs. [source]