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Haemoglobin Electrophoresis (haemoglobin + electrophoresis)
Selected AbstractsIncidence of iron-deficiency anaemia in infants in a prospective study in JordanEUROPEAN JOURNAL OF HAEMATOLOGY, Issue 4 2000Julia Kilbride Abstract: A high prevalence of iron-deficiency anaemia has been reported in Jordanian infants. A prospective study of infants in downtown Amman examined the relationship between anaemia in pregnancy and iron deficiency in infancy. The iron status of infants born to 107 anaemic (Hb <11 g/dl) and 125 non-anaemic mothers was reviewed at 3, 6, 9 and 12 months. Indicators to define iron-deficiency anaemia were Hb <11 g/dl and either plasma ferritin <12 ,g/l or zinc protoporphyrin (ZPP) >35 ,g/dl whole blood. Haemoglobin electrophoresis excluded haemoglobinopathy. There was 72% iron-deficiency anaemia throughout the year, significantly higher in infants born to anaemic mothers (81%; n=91) compared with controls (65%; n=112). At 12 months, 72% of the infants tested (n=195) were anaemic. While 57% were identified as iron-deficient by research criteria of either ferritin or ZPP, only 37% were identified by ferritin alone, 40% by ZPP alone and 29% if both ferritin and ZPP were required to meet criteria. Most infant anaemia was identified as due to iron deficiency, supporting contextual setting as assisting diagnosis: infants in developing countries are recognised as vulnerable to iron deficiency. Using multiple criteria, more cases were identified when either ferritin or ZPP were abnormal than when one alone, or both parameters were required to meet research criteria. [source] Haemoglobin Etobicoke, an incidental finding in an Irish diabeticINTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, Issue 4 2003D. A. O'Brien Summary It is well recognized that haemoglobin variants can be detected during the measurement of HbA1c by high-performance liquid chromatography (HPLC). A number of variants have been reported as compromising the quantification of HbA1c, a marker used in the assessment of glycaemic control in diabetes. We describe a case of haemoglobin Etobicoke, a rare alpha chain variant detected in an Irish diabetic during HbA1c analysis. Its identity was confirmed using a series of investigations. These included haemoglobin electrophoresis at alkaline and acid pH, isoelectric focusing and globin chain electrophoresis. Ultimately mass spectrometry isolated the mutation at position alpha 84 (F5). Haemoglobin Etobicoke, first described in Canada in 1969 has not previously been detected on HbA1c analysis. In the presence of this rare variant, HbA1c, a standard method using HPLC to assess glycaemic control in diabetes is unreliable and alternatives such as fructosamine need to be considered. HbA1c measured by automated HPLC will effectively screen populations where haemoglobin variants were not previously known. Precise identity of these variants when they are detected is crucial to the reliable interpretation of HbA1c analyses. [source] The frequency of the sickle allele in Jamaica has not declined over the last 22 yearsBRITISH JOURNAL OF HAEMATOLOGY, Issue 6 2005N. A. Hanchard Summary The ,malaria hypothesis' predicts that the frequency of the sickle allele, which is high in malaria-endemic African populations, should decline with each generation in populations of African descent living in areas where malaria is no longer endemic. In order to determine whether this has been the case in Jamaica, we compared haemoglobin electrophoresis results from two hospital-based screening programmes separated by more than 20 years (i.e. approximately one generation). The first comprised 100 000 neonates screened between 1973 and 1981, the second, 104 183 neonates screened between 1995 and 2003. The difference in frequency of the sickle allele was small (5·47% in the first cohort and 5·38% in the second screening cohort) and not significant (Z = 1·23, P = 0·22). The same was true of the sickle trait frequency (10·05% in the first cohort and 9·85% in the second, Z = 1·45, P = 0·15). These differences were smaller than predicted under simple deterministic models based on the malaria hypothesis, and suggest that these models may not capture important determinants of allele and trait frequency decline (or persistence) in contemporary populations. Refining the expectations for allele and trait frequency change for Jamaica and other similar populations is an area for future study. [source] An unusual application of haemoglobin electrophoresisBRITISH JOURNAL OF HAEMATOLOGY, Issue 2 2000Images in haematology No abstract is available for this article. [source] | ||||||||||