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Selected AbstractsEfficacy and toxicity of orally administered anticoccidial drugs for innovative treatments of Polysporoplasma sparis (Sitja-Bobadilla and Alvarez-Pellitero 1985) infection in Sparus aurata L.JOURNAL OF APPLIED ICHTHYOLOGY, Issue 5 2004F. Athanassopoulou Summary The purpose of this study was to test experimentally different drugs and therapeutic schemes in order to find a efficient commercial treatment for fish infected with myxosporeans. Two series of land-based experiments and one experimental cage trial were performed for this purpose. In the first land-based experiment, 10 and 30 g Sparus aurata naturally infected in the kidneys with Polysporoplasma sparis were used. Initially, six different doses of Fumagillin, two doses of Toltrazuril, and one dose of Amprolium, ESB3 and Salinomycin were tested. In the second land-based experiment, 25 and 50 g fish infected with the same parasite were treated with Origanum essential oils, Toltrazuril with propylene glycol, Amprolium, and a combination of Salinomycin 12% + Amprolium (SA). In the field trials, 15 and 155 g S. aurata infected with the same parasite were treated with SA, Origanum essential oils and Fumagillin. In all trials the drugs were incorporated in food and administered according to the selected schemes, while their efficacy was evaluated in terms of mortality (acceptable level <2%), pathology and prevalence rate of P. sparis. According to our results the SA combination proved to be the most effective treatment against P. sparis infection in S. aurata: (i) the therapeutic scheme and commercial product used was not toxic and (ii) a significant reduction in percentage of prevalence was observed. [source] Pharmacokinetics of oxolinic acid in gilthead sea bream, Sparus aurata L.JOURNAL OF FISH DISEASES, Issue 7 2002G Rigos This is the first study on the pharmacokinetic parameters of oxolinic acid (OA) in gilthead sea bream, Sparus aurata L. The kinetic profile of OA was studied after a single intravascular injection (20 mg kg,1) in 100 g fish at 20 °C. The distribution half-life (t1/2,) and the elimination half-life (t1/2,) of the drug were found to be short (0.51 and 12.60 h, respectively). The drug penetration from the plasma to the tissues was adequate as the apparent volume of distribution of the drug at steady-state (Vd(ss)) was found to be 2.11 L kg,1. The mean residence time (MRT) of OA was short (14.25 h) and the total clearance rate (ClT) of the drug was low (0.15 L kg,1 h,1). The bioavailability (F,%) of OA following oral administration (30 mg kg,1) was also low (14%). Maximum values were observed for muscle at 0.5 h after injection, with levels declining as with subsequent sampling. At the first two time points (0.5 and 1 h) plasma levels of OA were higher than muscle, however, the reverse was evident for subsequent samples. Following oral administration, highest muscle levels were found at 16 h and, with the exception of the 24-h sampling, muscle OA concentrations were higher than plasma at all time points. The fast elimination of OA suggests short withdrawal times with reference to human consumption of treated fish. [source] Effects of dietary starches and the protein to energy ratio on growth and feed efficiency of juvenile cobia, Rachycentron canadumAQUACULTURE NUTRITION, Issue 5 2010K.A. JR WEBB Abstract Optimization of the protein to energy ratio in juvenile cobia (Rachycentron canadum) would allow the production of diets that maximize growth without the addition of excess energy that may increase costs or even be detrimental to the health of the fish. During a 6-week growth trial, juvenile cobia (5.6 ± 0.5 g fish,1 initial weight) were fed five isonitrogenous and isolipidic diets containing various protein to energy ratios using starch as the energy source. At the end of the trial, some fish were analysed for body composition characteristics while the rest were used to examine the excretion of dietary starch in the feces. Survival and growth were not significantly affected, but feed efficiency (ranging from 0.64 to 0.94) and daily consumption (ranging from 45.3 to 64.1 g kg,1 of body weight d,1) were affected. No reduction in consumption due to excess energy was noted. Analysis of the fecal carbohydrate data showed a linear relationship between dietary inclusion and excretion of carbohydrates with no sign of reaching saturation. Results of this study suggest that cobia can utilize dietary carbohydrates up to at least 340 g kg,1 of dry diet with an optimal protein to energy ratio of approximately 34 mg protein kJ,1metabolizable energy. [source] The potential of land animal protein ingredients to replace fish meal in diets for cuneate drum, Nibea miichthioides, is affected by dietary protein levelAQUACULTURE NUTRITION, Issue 1 2010Y. WANG Abstract A net pen experiment was carried out to examine the effect of dietary protein level on the potential of land animal protein ingredients as fish meal substitutes in practical diets for cuneate drum Nibea miichthioides. Two isocaloric basal (control) diets were formulated to contain 400 g kg,1 herring meal but two different digestible protein (DP) levels (400 versus 350 g kg,1). At each DP level, dietary fish meal level was reduced from 400 to 280, 200, 80 and 0 g kg,1 by incorporating a blend that comprised of 600 g kg,1 poultry by-products meal (PBM), 200 g kg,1 meat and bone meal (MBM), 100 g kg,1 feather meal (FEM) and 100 g kg,1 blood meal (BLM). Cuneate drum fingerling (initial weight 42 g fish,1) were fed the test diets for 8 weeks. Fish fed the test diets exhibited similar feed intake. Final body weight, feed conversion ratio and nitrogen retention efficiency was not significantly different between fish fed the basal diets containing 350 and 400 g kg,1 DP. Weight gain decreased linearly with the reduction of dietary fish meal level at the 350 g kg,1 DP level, but did not decrease with the reduction of dietary fish meal level at the 400 g kg,1 DP level. Results of the present study suggest that fish meal in cuneate drum diets can be completely replaced with the blend of PBM, MBM, FEM and BLM at the 400 g kg,1 DP level, based on a mechanism that excessive dietary protein compensate lower contents of bio-available essential amino acid in the land animal protein ingredients relative to fish meal. [source] Dietary vitamin E requirement of the red drum Sciaenops ocellatusAQUACULTURE NUTRITION, Issue 3 2009L.I. PENG Abstract A 12-week feeding trial was conducted to establish the minimum dietary vitamin E requirement of juvenile red drum by broken-line regression analysis. The semi-purified basal diet was supplemented with 10, 20, 30, 40, 60 or 80 IU vitamin E kg,1 as all-rac -,-tocopheryl acetate. Juvenile red drum were conditioned by feeding the basal diet for 8 weeks prior to the feeding trial to reduce whole-body vitamin E levels. Then, fish initially averaging 12.2 ± 0.4 g fish,1 (mean ± SD) were fed the experimental diets at a rate approaching apparent satiation for 12 weeks. Weight gain and feed efficiency responses of fish fed diets were significantly (P < 0.01) altered by the level of vitamin E supplementation but not strictly in a dose-dependent manner. Vitamin E concentrations in liver and plasma also were significantly (P < 0.001) influenced by dietary vitamin E level. Plasma ascorbic acid in fish fed the basal diet tended (P = 0.066) to be lower than in fish fed diets containing the various levels of vitamin E. In addition, fish fed the basal diet showed edema in the heart, while fish fed all other diets were normal. Fish fed 60 or 80 IU all-rac -,-tocopheryl acetate kg,1 diet had significantly higher respiratory burst of head kidney macrophages than fish fed all other diets, although dietary effects on hematocrit and neutrophil oxidative radical production were not significant. The minimum dietary vitamin E requirement of juvenile red drum was established based on broken-line regression of liver thiobarbituric acid reactive substances to be 31 mg all-rac -,-tocopheryl acetate kg,1 diet. [source] Protein requirements of Atlantic cod Gadus morhua L.AQUACULTURE RESEARCH, Issue 3 2010Jón Árnason Abstract Three feeding trials were conducted to estimate the minimum protein requirements for maximum growth of Atlantic cod (initial size 37,600 g). The diets in each trial were near iso-energetic and contained 47,64% crude protein (CP) in dry matter for small fish while diets for larger fish contained 36,57% CP. There was no significant difference in the final weight of small fish (90,130 g) fed different levels of CP. However, the specific growth rate (SGR) of fish fed 59% and 64% CP (0.9% day,1) was significantly higher than that of fish fed either 47% or 48% CP (0.5,0.7% day,1). The hepato-somatic index (HSI) increased progressively with decreased CP and the feed conversion ratio (FCR) was negatively correlated with CP of the diets. In two trials with larger fish, CP did not significantly affect mean final weight (750,900 g), SGR or FCR. As for the smaller fish, the HSI was negatively correlated with CP and in one of the experiments, the feed intake was also negatively correlated with CP. The feed efficiency tended to be reduced. The results suggest that the protein requirement for maximum growth in Atlantic cod is size dependent: for 40,107 g fish, the minimum CP is 47,52% and for larger fish (400,900 g) it is 36% or lower. [source] Inclusion of macroalgae meal (Macrocystis pyrifera) as feed ingredient for rainbow trout (Oncorhynchus mykiss): effect on flesh fatty acid compositionAQUACULTURE RESEARCH, Issue 1 2009Patricio Dantagnan Abstract The use of macroalgae as an additional component in animal feeding has been studied. However, information on how it could influence muscle composition of fish body is scarce. This study evaluates four diets with different macroalgae inclusion levels (0%, 1.5%, 3% and 6%) to test the effect on body fatty acid composition of rainbow trout. Tanks with a volume of 600 L were stocked with 60.6 ± 7.9 g fish at a density of 45 individuals tank,1 and fed for 124 days. At the end of the experiment there were not significant differences (P<0.05) in muscle proximate composition among fish fed the different treatments. However, it was determined that inclusion of 3% and 6% of macroalgae meal resulted in a significant increase (P<0.05) of polyunsaturated fatty acids (PUFAs) in muscle. In summary, macroalgae meal in rainbow trout diets do not enhance the quantity of protein and lipid contents at muscle level but an addition of 3,6% might contribute to increase the level of PUFAs, specially EPA, DHA and LIN. Thus, use of macroalgae meal might help to increase lipid quality content in the final product due the beneficial effects of PUFAs for human health. [source] Evaluation of rendered animal protein ingredients for replacement of fish meal in practical diets for gibel carp, Carassius auratus gibelio (Bloch)AQUACULTURE RESEARCH, Issue 14 2008Menghong Hu Abstract A 12-week feeding trial was carried out in fibreglass tanks to examine partial replacement of fish meal (FM) with poultry by-product meal (PBM), meat and bone meal (MBM) and blood meal (BM) in practical diets for gibel carp Carassius auratus gibelio (Bloch). Triplicate groups of fish (initial body weight 15.3 g fish,1) were fed eight isonitrogenous (crude protein: 37.5%) and isolipidic diets (crude lipid: 7%). The control diet is the commercial diet of gibel carp, which used 18% of FM as the sole animal protein source. In the other seven diets (Diet 2,Diet 8), 17,83% of FM protein was substituted by a blend of PBM and BM or a blend of PBM, MBM and BM. The final body weight and thermal-unit growth coefficient of fish fed the feeds in Diet 8 in which 83% of FM protein was replaced by the blend of 3% BM, 10% PBM and 5% MBM is significantly lower than Diet 1 (the control). The feed efficiency ratio in Diet 8 group is significantly lower than Diet 1, 2, 4 and 7 groups. The results of the present study indicated that a combination of PBM, BM and MBM can replace most of the FM protein and the FM level can be reduced to about 6% with satisfactory growth and feed utilization in practical diets for gibel carp. [source] Evaluation of fisheries by-catch and by-product meals in diets for red drum Sciaenops ocellatus L.AQUACULTURE RESEARCH, Issue 16 2005Kasey W Whiteman Abstract This study evaluated various by-catch and by-product meals of marine origin with red drum (Sciaenops ocellatus L.). Four different kinds of by-catch or by-product meals [shrimp by-catch meal from shrimp trawling, Pacific white shrimp (Litopenaeus vannamei (Boone)) processing waste meal, red salmon (Oncorhynchus nerka (Walbaum)) head meal, and Pacific whiting (Merluccius productus (Ayres)) meal] were substituted for Special SelectÔ menhaden fish meal at 33% or 67% of crude protein in diets formulated to contain 40% crude protein, 12% lipid, and 14.6 kJ digestible energy g,1. Each of these diets and three additional diets consisting of shrimp processing waste meal formulated on a digestible-protein basis and two Pacific whiting diets containing reduced levels of ash were also evaluated in two 6-week feeding trials with juvenile red drum (initial weight of 4,5 and 1,2 g fish,1 in trials 1 and 2). Red drum fed by-catch meal at either level of substitution performed as well as fish fed the control diet; whereas, fish fed shrimp processing waste meal diets had significantly (P,0.05) reduced weight gain and feed efficiency ratio values compared with the controls, even when fed on a digestible-protein basis. The diets containing Pacific whiting at either levels of substitution and regardless of ash level supported similar performance of red drum as those fed the control diet. Fish fed the red salmon head meal diet fared poorly, probably owing to an excessive amount of lipid in the diet that became rancid. Overall, by-catch meal associated with shrimp trawling and Pacific whiting appear to be suitable protein feedstuffs for red drum. [source] Dietary Supplementation of a Purified Nucleotide Mixture Transiently Enhanced Growth and Feed Utilization of Juvenile Red Drum, Sciaenops ocellatusJOURNAL OF THE WORLD AQUACULTURE SOCIETY, Issue 2 2007Peng Li Thus, we investigated effects of a purified nucleotide mixture on growth and health of young red drum. The nucleotide premix, containing salts of cytidine, uridine, adenosine, and guanidine, was coated with binders, freeze-dried, and grounded to powder. A fish-meal-based diet was supplemented with 0.03, 0.1, or 0.3% by weight of the coated nucleotide mixture or with 0.2% Optimûn® (Chemoforma Co., Basel, Switzerland), a commercial nucleotide product. The experimental diets were maintained isonitrogenous and isocaloric by adjusting amounts of casein, gelatin, and alanine. Five replicate groups of 12 juvenile red drum (10.2 ± 0.2 g/fish, mean ± SD) were fed each experimental diet for 4 wk, followed by an assay of neutrophil oxidative radical production and a bacterial challenge via intraperitoneal injection of Vibrio harveyi at 2.9 × 107 colony-forming units/g fish. Fish fed all diets supplemented with various levels of purified nucleotides showed significantly (P < 0.01) enhanced weight gain and feed efficiency during the first week of feeding compared to fish fed the basal diet. However, the dietary effects became less significant during the following 3 wk of feeding. The transient growth-enhancing effect of dietary nucleotides observed in the present study may explain the conventional controversy about nucleotide effects on fish growth. Dietary supplementation with nucleotides had no influence on terminal whole-body composition. [source] |