Fish Responses (fish + response)

Distribution by Scientific Domains


Selected Abstracts


Non-specific immune response of turbot, Scophthalmus maximus (L.), experimentally infected with a pathogenic Vibrio pelagius

JOURNAL OF FISH DISEASES, Issue 6 2003
L Villamil
Abstract The effect of a pathogenic Vibrio pelagius, isolated during a mass mortality of turbot larvae, on the non-specific immune response of turbot, Scophthalmus maximus (L.), macrophages was studied both in vitro and in vivo. The in vitro treatment of head kidney (HK) macrophages with viable V. pelagius caused a significant inhibition of the chemiluminescence (CL) response in comparison with untreated macrophages, while incubation with heat-killed bacteria did not affect this response. In vivo, the intraperitoneal injection of V. pelagius resulted in a significant inhibition of the CL response in infected fish at days 1 and 4 post-infection compared with the control fish response. The HK macrophage nitric oxide (NO) production was enhanced by in vitro incubation with intermediate doses of viable V. pelagius (5 × 103 and 5 × 104 bacteria mL,1) and higher doses of the heat-killed bacteria (5 × 104,5 × 106 bacteria mL,1). In both cases, the NO inhibitorN- , -nitro-L-arginine was capable of down-regulating the specific NO induction caused by incubation with the bacterial treatments. In contrast, incubation with ECPs at higher doses caused a reduction in NO production. In vivo, a significant enhancement in NO production was also observed in macrophage supernatants at day 10 post-infection. Lysozyme concentration in the serum was also significantly increased in the experimentally infected fish at days 4 and 10 post-injection. In addition, viable V. pelagius and its ECPs significantly reduced HK macrophage viability in vitro, whereas no significant differences in viability were observed during the incubation with heat-killed bacteria. As NO production was enhanced in the experimentally infected fish, the inhibitory effect of the NO donor, S-nitroso-acetyl-penicillamine (SNAP), was tested in vitro in a cell-free assay. The results showed that growth of V. pelagius was significantly inhibited using SNAP at a high concentration (1 mm). [source]


Altered reproduction in fish exposed to pulp and paper mill effluents: Roles of individual compounds and mill operating conditions

ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 3 2008
L. Mark Hewitt
Abstract For the last 20 years, studies conducted in North America, Scandinavia, and New Zealand have shown that pulp and paper mill effluents affect fish reproduction. Despite the level of effort applied, few leads are available regarding the factors responsible. Effluents affect reproduction in multiple fish species, as evidenced by decreased gonad size, decreased circulating and gonadal production of reproductive steroids, altered expression of secondary sex characteristics, and decreased egg production. Several studies also have shown that effluent constituents are capable of accumulating in fish and binding to sex steroid receptors/binding proteins. Studies aimed at isolating biologically active substances within the pulping and papermaking process have provided clues about their source, and work has progressed in identifying opportunities for in-mill treatment technologies. Following comparisons of manufacturing processes and fish responses before and after process changes, it can be concluded that effluent from all types of mill processes are capable of affecting fish reproduction and that any improvements could not be attributed to a specific process modification (because mills normally performed multiple modifications simultaneously). Improved reproductive performance in fish generally was associated with reduced use of molecular chlorine, improved condensate handling, and liquor spill control. Effluent biotreatment has been effective in reducing some effects, but biotreated effluents also have shown no difference or an exacerbation of effects. The role of biotreatment in relation to effects on fish reproduction remains unclear and needs to be resolved. [source]


Use of paired fathead minnow (Pimephales promelas) reproductive test.

ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 7 2006
Part 1: Assessing biological effects of final bleached kraft pulp mill effluent using a mobile bioassay trailer system
Abstract Reproductive effects have been recorded in wild fish in waters receiving pulp mill effluent (PME) since the mid to late 1980s. Laboratory assays with fathead minnow (FHM; Pimephales promelas) have been developed to better understand fish responses to PME. However, observations from laboratory studies have been variable, making it difficult to establish cause/effect relationships. A lack of environmental relevance in these laboratory studies may have contributed to the variability observed. The objectives of the present study were, first, to determine the effects of bleached kraft PME (BKME) on FHM under environmentally realistic conditions (i.e., ambient water and effluent quality) and, second, to determine the suitability of pair-breeding FHM to better link BKME-induced changes in indicators at the biochemical, individual, and population levels. A mobile bioassay trailer was situated on-site at a bleached kraft mill for 60 d, allowing supply of both ambient water (Lake Superior, Canada) and final BKME. The reproductive output of FHM was initially assessed for 21 d to obtain baseline data pre-exposure. At the end of the pre-exposure period, selected breeding pairs were exposed to final BKME (100% v/v and 1% v/v) for 21 d. Results demonstrated a stimulatory response pattern at 1% BKME (e.g., increased egg production) compared to control. In the 100% treatment, spawning events were reduced and fewer eggs were produced during the first two weeks of exposure. Exposure to 100% (v/v) BKME also resulted in ovipositor development in males and development of male secondary sex characteristics in females. Obtaining pre-exposure data and use of pair-breeding FHM in this assay gave a sensitive indication of effluent effects and allowed accurate comparisons of endpoints to be made. [source]


Forest change and stream fish habitat: lessons from ,Olde' and New England

JOURNAL OF FISH BIOLOGY, Issue 2005
K. H. Nislow
The North Atlantic region has a long history of land use change that has influenced and will continue to influence stream ecosystems and fisheries production. This paper explores and compares the potential consequences of changes in forest cover for fish production in upland, coldwater stream environments in New England, U.S.A. and the British Isles, two regions which share important similarities with respect to overall physical, biotic and socio-economic setting. Both regions were extensively deforested and essentially no extensive old-growth forest stands remain. In New England, recovering forests, consisting almost entirely of naturally-regenerated native species, now cover >60% of the landscape. Associated with this large-scale reforestation, open landscapes, common in the 19th and first half the 20th century, are currently rare and declining in this region. In the British Isles, forests still cover <20% of the landscape, and existing forests largely consist of exotic conifer plantations stocked at high stand densities and harvested at frequent rotations. While forest restoration and conservation is frequently recommended as a fisheries habitat conservation and restoration tool, consideration of the way in which forests affect essential aspects of fish habitat suggests that response of upland stream fish to landscape change is inherently complex. Under certain environmental settings and reforestation practices, conversion of open landscapes to young-mature forests can negatively impact fish production. Further, the effects of re-establishing old-growth forests are difficult to predict for the two regions (due to the current absence of such landscapes), and are likely to depend strongly on the extent to which critical ecosystem attributes (large-scale disturbances, fish migrations, keystone species, large woody debris recruitment) are allowed to be re-established. Understanding these context-dependencies is critical for predicting fish responses, and should help managers set realistic conservation, management and restoration goals. Management may best be served by promoting a diversity of land cover types in a way that emulates natural landscape and disturbance dynamics. This goal presents very different challenges in New England and the British Isles due to differences in current and predicted land use trajectories, along with differences in ecological context and public perception. [source]