			Home About us Contact

Fish Liver (fish + liver)

Distribution by Scientific Domains

Earth and Environmental Science	37%
Chemistry	37%
Life Sciences	25%

Selected Abstracts

Chemical Characterization of Liver Lipid and Protein from Cold-Water Fish Species

JOURNAL OF FOOD SCIENCE, Issue 6 2006
Peter J. Bechtel
ABSTRACT:, The largest US harvests of marine fish for human consumption are from Alaska waters. Livers from these fish are combined with other fish offal and made into fish meal and oil or discarded. The purpose of this study was to characterize liver lipids and proteins from important commercial species including walleye pollock (WP), pink salmon (PS), Pacific halibut (PH), flat head sole (FS), and spiny head rock fish (RF), and underutilized species including arrow tooth flounder (AF) and big mouth sculpin (BS). Liver lipid content ranged from 50.3% in WP to 3.3% in PS. Protein content ranged from 7.7% in WP to 18.4% in BS. PS livers had the highest content of ,-3 fatty acids at 336 mg/g of oil and AF had the lowest content at 110 mg/g of oil. There were significant differences in the content of 9 amino acids with methionine and lysine values ranging from 2.66% to 3.43% and 7.19% to 9.45% of the total amino acids, respectively. Protein from the cold water marine fish livers was of high quality and the oils contained substantial quantities of ,-3 fatty acids. Fish livers had distinct chemical properties and can be used for the development of unique food ingredients. [source]

Bioaccumulation and ROS generation in liver of freshwater fish, goldfish Carassius auratus under HC Orange No. 1 exposure

ENVIRONMENTAL TOXICOLOGY, Issue 3 2007
Yuanyuan Sun
Abstract HC Orange No. 1 (CAS No. 54381-08-7, 2-nitro-4,-hydroxydiphenylamine) is used as a color additive in hair dyes. In this study, laboratory experiment was carried out to determine HC Orange No. 1 bioaccumulation and oxidative stress in the liver of freshwater fish, goldfish Carassius auratus. Fish were exposed to different concentrations (0.05, 0.1, 0.2, 0.5, and 1.0 mg/L) of HC Orange No. 1 for 10 days, with one group assigned as control. The accumulation of HC Orange No. 1 in liver increased with the exposure concentration (R2 = 0.94). A secondary spin trapping technique was used followed by electron paramagnetic resonance (EPR) analysis to study the reactive oxygen species (ROS) production. On the basis of the hyperfine splitting constants and shape of the EPR spectrum, the ROS generated in fish liver after exposure was identified as hydroxyl radical (,OH). There is a good correlation between the exposure concentrations and ,OH generation (R2 = 0.92). The ,OH signal intensity of the EPR spectrum showed a significant increase (P < 0.05) when the HC Orange No. 1 concentration was 1.0 mg/L, compared with that of the control. A good positive relationship (R2 = 0.95) was found between the ,OH formation and accumulation level of HC Orange No. 1 in liver. The changes of the activities of catalase (CAT), superoxide dismutase (SOD), glutathione S -transferase (GST), and contents of reduced glutathione (GSH) were also detected. These observations indicated a possible mechanism of oxidative stress induced by HC Orange No. 1 on fish. © 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 256,263, 2007. [source]

Effects of lipid-lowering pharmaceuticals bezafibrate and clofibric acid on lipid metabolism in fathead minnow (Pimephales promelas),

ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 12 2009
Anna Weston
Abstract The lipid-lowering agents bezafibrate and clofibric acid, which occur at concentrations up to 3.1 and 1.6 ,L, respectively, are among the most frequently found human pharmaceuticals in the aquatic environment. In contrast to knowledge about their environmental occurrence, little is known about their effects in the environment. The aim of the present study was to analyze effects of these lipid-lowering agents in fish by focusing on their modes of action, lipid metabolism. Fathead minnows were exposed in aquaria to measured concentrations of 0.1, 1.27, 10.18, 101.56, and 106.7 mg/L bezafibrate and to 1.07, 10.75, and 108.91 mg/L clofibric acid for 14 and 21 d, respectively. After exposure, fish liver was analyzed for expression of peroxisome proliferator-activated receptor , (PPAR,) by quantitative polymerase chain reaction (PCR), and the PPAR-regulated enzyme fatty acyl-coenzyme-A oxidase (FAO) involved in fatty acid oxidation. Bezafibrate had no effect, either on PPAR, expression or on FAO activity, at all concentrations. In contrast, clofibric acid induced FAO activity in male fathead minnows at 108.91 mg/L. No increase in expression of PPAR, messenger ribonucleic acid was observed. Egg production was apparently decreased after 21 d of exposure to 108.91 mg/L clofibric acid. The present study demonstrates that bezafibrate has very little or no effect on PPAR, expression and FAO activity, but clofibric acid affects FAO activity. [source]

Interaction of Phytochemical-Quercetin with the Other Antioxidant, Ascorbic Acid and their Protective Effect in Tilapia after Ultraviolet Irradiation

JOURNAL OF THE WORLD AQUACULTURE SOCIETY, Issue 5 2009
Gustavo A. Rodriguez-Montes De Oca
Semi-purified, casein-gelatin-based diets were prepared and supplemented with quercetin (Q) and/ or ascorbic acid (AA): control diet C,Q,(100 mg/kg AA), diet C ,Q+ (100 mg/kg AA + quercetin 10 g/kg), diet C +Q, (1000 mg/kg AA), and diet C +Q+ (1000 mg/kg AA + quercetin 10 g/kg). These diets were fed to tilapia for 19 wk and then fish were divided into controls and ultraviolet (UV) treatments. Fish were exposed to UV radiation. Control groups were protected with a MYLAR® polyester film and plexiglass. At week 20, the same fish were re-exposed to UV radiation. Control groups of fish were protected by a double layer of MYLAR® and the UV groups were exposed with no protection. Before UV exposure, 24 h after, and 7 d after the second treatment, fish liver and skin were dissected for Q and AA analyses. The proportion of oxidized ascorbate was significantly increased in fish from treatments C ,Q, and C ,Q+ . Q concentrations in fish after exposures were negligible in skin, whereas liver concentrations were significantly different among control (34 ± 10 ,g/g) and UV-irradiated fish (11 ± 6 ,g/g), respectively. The interaction between these two dietary antioxidants may change after chronic UV irradiation. [source]

Role of dietary patterns for dioxin and PCB exposure

MOLECULAR NUTRITION & FOOD RESEARCH (FORMERLY NAHRUNG/FOOD), Issue 11 2009
Helen E. Kvalem
Abstract Dietary patterns were related to intake and blood concentrations of polychlorinated dibenzo- p -dioxins and dibenzofurans (PCDD/PCDFs), dioxin-like polychlorinated biphenyls (dl-PCBs) and selected non-dioxin-like-PCBs (ndl-PCBs). Intake calculations were based on an extensive food frequency questionnaire and a congener-specific database on concentrations in Norwegian foods. The study (2003) applied a two-step inclusion strategy recruiting representative (n=73) and high consumers (n=111) of seafood and game. Estimated median intakes of sum PCDD/PCDFs and dl-PCBs of the representative and high consumers were 0.78 and 1.25 pg toxic equivalents (TEQ)/kg bw/day, respectively. Estimated median intakes of ndl-PCBs (sum chlorinated biphenyl (CB)-28, 52, 101, 138, 153, 180) were 4.26 and 6.40 ng/kg bw/day. The median blood concentrations of PCDD/PCDFs/dl-PCBs were 28.7 and 35.1 pg TEQ/g lipid, and ndl-PCBs (sum of CB-101, 138, 153 and 180) 252 and 299 ng/g lipid. The Spearman correlations between dietary intake and serum concentration were r=0.34 (p=0.017) for dl-compounds and r=0.37 (p<0.001) for ndl-PCBs. Oily fish was the major source of dl-compounds and ndl-PCBs in high and representative consumers. Four dietary patterns were identified by principal component analysis. Two were related to high intakes, one dominated by oily fish ((,-3)), the other by fish liver and seagull eggs ("northern coastal"). Only the latter was closely associated with high blood concentrations of dioxins and PCBs. [source]

Histopathological alterations in the liver of the sharptooth catfish Clarias gariepinus from polluted aquatic systems in South Africa

ENVIRONMENTAL TOXICOLOGY, Issue 2 2009
M. J. Marchand
Abstract There is a need for sensitive bio-monitoring tools in toxicant impact assessment to indicate the effect of toxicants on fish health in polluted aquatic ecosystems. Histopathological assessment of fish tissue allows for early warning signs of disease and detection of long-term injury in cells, tissues, or organs. The aim of this study was to assess the degree of histopathological alterations in the liver of C. gariepinus from two dams in an urban nature reserve, (Gauteng, South Africa). Two dams (Dam 1 and Dam 2) were chosen for their suspected levels of toxicants. Water and sediments were sampled for metal and potential endocrine disrupting chemical analysis. A quantitative and qualitative histology-based health assessment protocol was employed to determine the adverse health effects in fish. The analysis of blood constituents, fish necropsy, calculation of condition factors, and hepatosomatic indices were employed to support the findings of the qualitative and quantitative histological assessment of liver tissue. Assessment of the liver tissue revealed marked histopathological alterations including: structural alterations (hepatic cord disarray) affecting 27% of field specimens; plasma alterations (granular degeneration 98% and fatty degeneration 25%) of hepatocytes; an increase in melanomacrophage centers (32%); hepatocyte nuclear alterations (90%); and necrosis of liver tissue (14%). The quantitative histological assessment indicated that livers of fish collected from Dam 1 were more affected than the fish livers collected from Dam 2. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009. [source]

Proteomics analysis of liver samples from puffer fish Takifugu rubripes exposed to excessive fluoride: An insight into molecular response to fluorosis

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, Issue 1 2010
Jian Lu
Abstract Comparative proteomics was performed to identify proteins in the liver of Takifugu rubripes in response to excessive fluoride exposure. Sixteen fish were randomly divided into a control group and an experimental group. The control group was raised in soft water alone (F, = 0.4 mg/L), and the experimental group was raised in the same water with sodium fluoride at a high concentration of 35 mg/L. After 3 days, proteins were extracted from the fish livers and then subjected to two-dimensional polyacrylamide gel electrophoresis analysis. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to identify the proteins that were differentially expressed from the two groups of fish. Among an average of 816 and 918 proteins detected in the control and treated groups, respectively, 16 proteins were upregulated and 35 were downregulated (P < 0.01) in the fluoride-treated group as compared with those in the control group. Twenty-four highly differentially expressed proteins were further analyzed by MALDI-TOF/TOF-MS, and eight were identified by Mascot. These eight proteins include disulfide isomerase ER-60, 4SNc-Tudor domain protein, SMC3 protein, Cyclin D1, and mitogen-activated protein kinase 10, as well as three unknown proteins. Consistent with their previously known functions, these identified proteins seem to be involved in apoptosis and other functions associated with fluorosis. These results will greatly contribute to our understanding of the effects of fluoride exposure on the physiological and biochemical functions of Takifugu and the toxicological mechanism of fluoride causing fluorosis in both fish and human. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:21,28, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20308 [source]