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First Isolation (first + isolation)
Selected AbstractsFirst Isolation of Both Indolylcarbinols and Diindolylalkanes from Microwave-Assisted Acid (Clay)-Catalyzed Reaction of Indoles with Diethyl Ketomalonate.CHEMINFORM, Issue 21 2005Manas Chakrabarty Abstract For Abstract see ChemInform Abstract in Full Text. [source] First Isolation and Spectroscopic Observation of Thiofulminic acid (HCNS)CHEMISTRY - A EUROPEAN JOURNAL, Issue 25 2009Tibor Pasinszki Prof. Abstract For the first time: Thiofulminic acid (HCNS), the parent member of the nitrile sulfide family of reactive intermediates and potential interstellar species, was produced and characterized by IR spectroscopy for the first time. HCNS was generated in cryogenic matrices by 254,nm UV irradiation of 1,2,5-thiadiazole (see figure). [source] Isolation of a Carnobacterium maltaromaticum- like bacterium from systemically infected lake whitefish (Coregonus clupeaformis)FEMS MICROBIOLOGY LETTERS, Issue 1 2008Thomas P. Loch Abstract Herein we report on the first isolation of a Carnobacterium maltaromaticum -like bacterium from kidneys and swim bladders of lake whitefish (Coregonus clupeaformis) caught from Lakes Michigan and Huron, Michigan. Isolates were Gram-positive, nonmotile, facultatively anaerobic, asporogenous rods that did not produce catalase, cytochrome oxidase, or H2S, and did not grow on acetate agar. Except for carbohydrate fermentation, many phenotypic characteristics of lake whitefish isolates coincided with those of C. maltaromaticum, the causative agent of pseudokidney disease. Partial sequencing of 16S and 23S rRNA genes, as well as the piscicolin 126 precursor gene, yielded 97% and 98% nucleotide matches with C. maltaromaticum, respectively (accession numbers EU546836 and EU546837; EU643471). Phylogenetic analyses showed that lake whitefish isolates of this study are highly related, yet not fully identical to C. maltaromaticum. The presence of the C. maltaromaticum -like bacterium was associated with splenomegaly, renal and splenic congestion, and thickening of the swim bladder wall with accumulation of a mucoid exudate. Examination of stained tissue sections revealed renal and splenic congestion, vacuolation and bile stasis within the liver, and hyperplasia within the epithelial lining of the swim bladder. The concurrent presence of pathological changes and the C. maltaromaticum -like bacteria suggests that this bacterium is pathogenic to lake whitefish. [source] Novel results obtained by freezing berry pseudorotation of phosphoranes (10-P-5)HETEROATOM CHEMISTRY, Issue 5 2002Kin-ya Akiba By freezing Berry pseudorotation of spirophosphoranes with recourse to the rigidity of the Martin bidentate ligand, we successfully prepared configurationally stable enantiomeric pairs of optically active phosphoranes, and could isolate "anti-apicophilic" C-apical O-equatorial (O-cis) phosphoranes. The effect of ,*PO orbital of the O-cis phosphorane was investigated both experimentally and theoretically. O-cis phosphoranes were revealed to be much more electrophilic at the phosphorus atom than O-trans isomers by experimental studies. The acidity of the ,-proton of an O-cis benzylphosphorane was found to be higher than that of the corresponding O-trans isomer. By the reaction of the ,-carbanion of an O-cis benzylphosphorane with PhCHO, we succeeded in the first isolation and full structural characterization of a 12-P-6 phosphate bearing an oxaphosphetane ring, the intermediate in the Wittig type reaction using a 10-P-5 phosphorane. © 2002 Wiley Periodicals, Inc. Heteroatom Chem 13:390,396, 2002; Published online in Wiley Interscience (www.interscience.wiley.com). DOI 10.1002/hc.10072 [source] First description of non-motile Yersinia ruckeri serovar I strains causing disease in rainbow trout, Oncorhynchus mykiss (Walbaum), cultured in SpainJOURNAL OF FISH DISEASES, Issue 6 2006B Fouz Abstract Yersinia ruckeri, the causal agent of enteric redmouth (ERM) disease, was isolated from epizootics that occurred in different Spanish rainbow trout, Oncorhynchus mykiss (Walbaum), farms in which vaccination against ERM had been performed. In all episodes, the most pronounced clinical signs exhibited by affected fish were severe haemorrhages in the mouth, eyes and around the vent. The isolates were identified as Y. ruckeri serovar I by 16S rRNA sequencing together with serological tests. They lacked motility and lipase activity and thus belonged to biotype 2, and were highly virulent for juvenile rainbow trout, both by intraperitoneal injection (from 3.1 × 102 to 6.3 × 103 cfu per fish) and bath challenge (5.1,7.3 × 106 cfu mL,1). This is the first description of Y. ruckeri serovar I biotype 2 causing disease in cultured trout in Spain vaccinated with commercial ERM vaccines. The occurrence of this emergent pathogen in Spanish continental aquaculture from its first isolation in 2001 to date is also documented. [source] Antibody response to Pseudomonas aeruginosa in children with cystic fibrosisPEDIATRIC PULMONOLOGY, Issue 4 2009Lucimar G. Milagres PhD Abstract Cystic fibrosis (CF) is the most frequent life threatening autosomal recessive disease in white subjects. The primary cause of morbidity and mortality in children with CF is chronic pulmonary infection, mainly caused by Pseudomonas aeruginosa. The purpose of this study was to assess the value of the measurement of antibodies to P. aeruginosa in diagnosing lung infection by the bacteria in CF patients. We assessed P. aeruginosa antibody titers in CF patients from Rio de Janeiro, Brazil, using cell lysate antigens as well as recombinant PcrV, a Type III Secretion System protein. Sputum (more than 70% of the specimens) or oropharyngeal swabs were obtained whenever patients were regularly followed for their pulmonary disease. Blood samples were obtained with an average interval of 6 months for a period of 2 years. The ELISA cut-offs were assigned as the positive 95% confidence interval of the mean antibody levels from non-fibrocystic controls. Our data showed that most CF patients (81%) of whom were not chronically infected by P. aeruginosa (Groups I and II), had their first serology positive for rPcrV. Cell-lysate ELISA was able to detect P. aeruginosa antibodies before positive culture in the first serum sample of 44% of the patients from Groups I and II. When serum reactivity to rPcrV and cell lysate were combined, 94% of CF patients from Groups I and II (n,=,16) had the first serology positive for P. aeruginosa over a mean time of 20 months before the first isolation of P. aeruginosa. In conclusion, longitudinal P. aeruginosa serology should become part of respiratory care follow-up, in conjunction with other lung parameter functions. Pediatr Pulmonol. 2009; 44:392,401. © 2009 Wiley-Liss, Inc. [source] Transmission of colistin-resistant Pseudomonas aeruginosa between patients attending a pediatric cystic fibrosis center,PEDIATRIC PULMONOLOGY, Issue 4 2002M. Denton MD Abstract We report on an outbreak of colistin-resistant Pseudomonas aeruginosa (CRPA) that occurred in a United Kingdom pediatric cystic fibrosis (CF) unit and involved six children over a period of 5 years. All CRPA-positive children had received aerosolized colistin therapy before first isolation of resistant organisms (mean duration, 3.1 years). Four of the 6 had also received courses of intravenous colistin in the year before the first isolation of CRPA. No impact of CRPA acquisition on respiratory function, clinical condition, or radiological parameters could be demonstrated. Four of the 6 children carried isolates of CRPA indistinguishable on genotyping. Two of these 4 children were sisters. The other 2 were on the same ward together at time of first isolation, and subsequently shared overlapping admissions with one of the sisters. While there is no conclusive evidence for the route of transmission, the frequency of overlapping in-patient admissions between 3 of these patients is suggestive of patient-to-patient transfer in the nosocomial setting. CF clinicians should be aware that colistin resistance can occur in P. aeruginosa, and some of these strains are capable of spread within CF units. Pediatr Pulmonol. 2002; 34:257,261. © 2002 Wiley-Liss, Inc. [source] Characterization of N-methyl-L-methionine sulfoxide and isethionic acid from the red alga Grateloupia doryphoraPHYCOLOGICAL RESEARCH, Issue 2 2002Christelle Simon-Colin SUMMARY Isethionic acid (2-hydroxyethane sulfonic acid) and N-methyl-L-methionine sulfoxide (4-methane sulfinyl-2-methylamino butyric acid) were isolated from the red alga Grateloupia doryphora (Cryptonemiales) collected from Brittany (France); they were identified as major organic solutes together with floridoside (,-D-galacto-pyranosyl-(1,2)-glycerol). The presence of isethionic acid has recently been reported in certain red algae, however, the occurrence of N-methyl-L-methionine sulfoxide is still very rare. This report deals with the first isolation of isethionic acid and N-methyl-L-methionine sulfoxide from G. doryphora and their subsequent NMR characterization. [source] Renal carcinogenesis: Genotype, phenotype and dramatypeCANCER SCIENCE, Issue 2 2003Okio Hino Cancer is a heritable disorder of somatic cells. Environment and heredity are both important in the carcinogenic process. The Eker rat model of hereditary renal carcinoma (RC) is an example of a Mendelian dominantly inherited predisposition to a specific cancer in an experimental animal. Forty years after the discovery of the Eker rat in Oslo, we and Knudson's group independently identified a germline retrotransposon insertion in the rat homologue of the human tuberous sclerosis (TSC2) gene. To our knowledge, this was the first isolation of a Mendelian dominantly predisposing cancer gene in a naturally occurring animal model. Recently, we discovered a new hereditary renal carcinoma in the rat. This rat was named the "Ninon''rat and its predisposing (Nihon) gene could be a novel renal tumor suppressor gene. This article will review the utility of these unique models for the study of problems in carcinogenesis; e.g., species-specific differences in tumorigenesis, cell stage and tissue/cell-type specific tumorigene-sis, multistep carcinogenesis, modifier gene(s) in renal carcinogenesis, cancer prevention and the development of therapeutic treatments which can be translated to human patients, as well as how environmental factors interact with cancer susceptibility gene(s). (Cancer Sci 2003; 94: 142,147) [source] Emergence of ciprofloxacin resistance in Escherichia coli isolates from outpatient urine samplesCLINICAL MICROBIOLOGY AND INFECTION, Issue 3 2007C. Gagliotti Abstract This study investigated the association between prescription of fluoroquinolones and emergence of ciprofloxacin resistance among Escherichia coli isolates in the urine of outpatients from whom a ciprofloxacin-sensitive E. coli strain had been isolated previously. Patients were identified and followed using the healthcare databases of Emilia-Romagna Region, Italy. The outcome of interest was the first isolation from urine of an E. coli strain resistant to ciprofloxacin. Prescription of fluoroquinolones during the previous 6 months was associated independently with the emergence of ciprofloxacin resistance; the strength of the association varied according to individual fluoroquinolone agents. [source] | ||||||||||||||||