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Extensive Tests (extensive + test)

Distribution by Scientific Domains
Life Sciences50%

Selected Abstracts

Optimal job splitting on a multi-slot machine with applications in the printing industry

NAVAL RESEARCH LOGISTICS: AN INTERNATIONAL JOURNAL, Issue 3 2010
Ali Ekici
Abstract In this article, we define a scheduling/packing problem called the Job Splitting Problem, motivated by the practices in the printing industry. There are n types of items to be produced on an m -slot machine. A particular assignment of the types to the slots is called a "run" configuration and requires a setup cost. Once a run begins, the production continues according to that configuration and the "length" of the run represents the quantity produced in each slot during that run. For each unit of production in excess of demand, there is a waste cost. Our goal is to construct a production plan, i.e., a set of runs, such that the total setup and waste cost is minimized. We show that the problem is strongly NP-hard and propose two integer programming formulations, several preprocessing steps, and two heuristics. We also provide a worst-case bound for one of the heuristics. Extensive tests on real-world and randomly generated instances show that the heuristics are both fast and effective, finding near-optimal solutions. © 2010 Wiley Periodicals, Inc. Naval Research Logistics, 2010 [source]

The impact of fluid-dynamic-generated stresses on chDNA and pDNA stability during alkaline cell lysis for gene therapy products

BIOTECHNOLOGY & BIOENGINEERING, Issue 4 2001
S. Chamsart
Abstract Extensive tests have been carried out to assess the impact of fluid-dynamic-generated stress during alkaline lysis of Escherichia coli cells (host strain DH1 containing the plasmid pTX 0161) to produce a plasmid DNA (pDNA) solution for gene therapy. Both a concentric cylinder rheometer and two stirred reactors have been used, and both the alkaline addition and neutralization stages of lysis have been studied. Using a range of shear rates in the rheometer, stirrer speeds in the reactors, and different periods of exposure, their impact on chromosomal DNA (chDNA) and pDNA was assessed using agarose gel electrophoresis, a Qiagen Maxiprep with a polymerase chain reaction (PCR) assay, and a Qiagen Miniprep purification with a UV spectrophotometer. Comparison has been made with unstressed material subjected to similar holding times. These tests essentially show that under all these conditions, <2% chDNA was present in the pDNA solution, the pDNA itself was not fragmented, and a yield of 1 mg/g cell was obtained. These results, together with studies of rheological properties, have led to the design of a 60-L, stirred lysis reactor and the production of high-quality pDNA solution with <1% chDNA after further purification. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75:387,392, 2001. [source]

Linking dispersal, immigration and scale in the neutral theory of biodiversity

ECOLOGY LETTERS, Issue 12 2009
Ryan A. Chisholm
Abstract In the classic spatially implicit formulation of Hubbell's neutral theory of biodiversity a local community receives immigrants from a metacommunity operating on a relatively slow timescale, and dispersal into the local community is governed by an immigration parameter m. A current problem with neutral theory is that m lacks a clear biological interpretation. Here, we derive analytical expressions that relate the immigration parameter m to the geometry of the plot defining the local community and the parameters of a dispersal kernel. Our results facilitate more rigorous and extensive tests of the neutral theory: we conduct a test of neutral theory by comparing estimates of m derived from fits to empirical species abundance distributions to those derived from dispersal kernels and find acceptable correspondence; and we generate a new prediction of neutral theory by investigating how the shapes of species abundance distributions change theoretically as the spatial scale of observation changes. We also discuss how our main analytical results can be used to assess the error in the mean-field approximations associated with spatially implicit formulations of neutral theory. Ecology Letters (2009) 12: 1385,1393 [source]

Bayesian estimation of traffic lane state

INTERNATIONAL JOURNAL OF ADAPTIVE CONTROL AND SIGNAL PROCESSING, Issue 1 2003
Ivan Nagy
Abstract Modelling of large transportation systems requires a reliable description of its elements that can be easily adapted to the specific situation. This paper offers mixture model as a flexible candidate for modelling of such element. The mixture model describes particular and possibly very different states of a specific system by its individual components. A hierarchical model built on such elements can describe complexes of big city communications as well as railway or highway networks. Bayesian paradigm is adopted for estimation of parameters and the actual component label of the mixture model as it serves well for the subsequent decision making. As a straightforward application of Bayesian method to mixture models leads to infeasible computations, an approximation is applied. For normal stochastic variations, the resulting estimation algorithm reduces to a simple recursive weighted least squares. The elementary modelling is demonstrated on a model of traffic flow state in a single point of a roadway. The examples for simulated as well as real data show excellent properties of the suggested model. They represent much wider set of extensive tests made. Copyright © 2003 John Wiley & Sons, Ltd. [source]