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European Committee (european + committee)
Selected AbstractsIn vitro susceptibility-testing in Aspergillus speciesMYCOSES, Issue 5 2008Cornelia Lass-Flörl Summary Aspergillus species are the most common causes of invasive mould infections in immunocompromised patients. The introduction of new antifungal agents and recent reports of resistance emerging during treatment of Aspergillus infections have highlighted the need for in vitro susceptibility-testing. Various testing procedures have been proposed, including macrodilution and microdilution, agar diffusion, disc diffusion and Etest. At present, one of the most widely used assays is the M38-A reference method for filamentous fungi, published by the Clinical Laboratory Standard Institute and the Etest. Recently, the European Committee on Antimicrobial Susceptibility-testing (EUCAST) has charged its Antifungal Susceptibility-testing Subcommittee (AFST-EUCAST) with the preparation of new guidelines for in vitro susceptibility-testing of antifungals against Aspergillus spp. (EUCAST-AFST-ASPERGILLUS) defining breakpoints. This paper reviews the available methods for antifungal susceptibility-testing in Aspergillus spp. as well as the scant data regarding the clinical implications of in vitro testing. [source] Antifungal susceptibility testing by flow cytometry: is it the future?MYCOSES, Issue 4 2006Luís André Vale-Silva Summary The current increase in the number and significance of fungal infections, the expanding armamentarium of antifungal agents, and the emergence of the problem of antifungal drug resistance have been intensifying the importance of antifungal susceptibility testing (AST). The Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS) in the United States and the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (AFST-EUCAST) published standard methodologies in order to achieve higher reproducibility and allow direct inter-laboratory comparison of the susceptibility results. Nevertheless, several problems remain unresolved and the methods depend on long incubation periods of a minimum of 24 h (EUCAST) or even 48 h (CLSI). Over the last 15 years, successful applications of flow cytometric techniques to AST of both yeast and moulds have been reported. These techniques are based on the analysis of a great number of fungal cells individually and frequently rely on short incubation times of no more than a few hours. Considering these attributes, flow cytometry (FC) seems to have the potential to achieve clinical usefulness in the near future. The collection of data on the reproducibility of the results and on the correlation with clinical outcomes has barely started, however. Practical validation of the experimental methodologies is not granted before a significant amount of data addressing those questions is available. [source] Evaluation of sustainable packaging in the norwegian shopping basket 2001,2007PACKAGING TECHNOLOGY AND SCIENCE, Issue 3 2010Hanne Møller Abstract The main purpose of the Norwegian Shopping Basket Survey (referred to here as the Survey) is to document development in packaging optimization and generation of packaging waste in the Norwegian packaging sector over time. As the environmental and resource efficiency of packaging systems depends to a large extent on the prevention of product loss, material intensity of the packaging and closing of the material loops, these factors are important elements in the characterization of sustainability of packaging systems. In this project we calculate indicators for material intensity and material recycling, based on the European Union (EU) directive on packaging and packaging waste (EC 64/92) and the corresponding European Committee for Standardization (CEN)-standards EN 13427-13432. The survey consists of 22 product categories that have a significant economic contribution to the retail sector. In each product category the three products with the highest turnover (market leaders) and the three products with the most rapid increase in turnover each year (market winners) are chosen. The survey result shows that the weighted packaging material intensity for the market leaders has been reduced but that the net material intensity has been rather constant. Data for the market winners indicate a much larger variation throughout the period, which is expected because many of the products change from year to year. The numbers of packaging changes are counted. Market changes are found to be the most important factor affecting the differences in material intensity. Changes in the packaging system of the individual products are less important. Copyright © 2010 John Wiley & Sons, Ltd. [source] Developing standard approaches for recording and assessing river hydromorphology: the role of the European Committee for Standardization (CEN)AQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue S1 2010Philip J. Boon Abstract 1. Over the last 20 years the concept of ,freshwater quality' has expanded to incorporate a range of physical, chemical, hydromorphological and biological characteristics. In Europe, this process has been given greater impetus by the EC Water Framework Directive (WFD) and the requirement for Member States to assess and monitor a comprehensive suite of freshwater features. 2. Assessments of river hydromorphology are needed not only for implementing the WFD but also for nature conservation purposes, such as monitoring the condition of Special Areas of Conservation under the EC Habitats Directive and helping in the management and restoration of rivers. At present there are few detailed methods available in Europe for assessing river hydromorphology, and standard approaches are urgently required. 3. The European Committee for Standardization (CEN) works to promote voluntary technical harmonization in Europe in conjunction with worldwide bodies and its European partners. The production of a CEN standard for assessing the hydromorphological features of rivers began in September 1999 and culminated in the production of EN 14614 in November 2004. 4. EN 14614 is a ,guidance standard' that provides a framework of general principles and sets out which aspects of river hydromorphology should be assessed, how to plan and conduct field surveys, how results should be interpreted and presented, and ways of applying quality assurance procedures. 5. A second CEN standard on river hydromorphology has recently been published. Using simple scoring systems, this will enable the extent of hydromorphological modification to river channels, banks, riparian zones and floodplains to be recorded and assessed consistently. 6. CEN standards encourage European collaboration and provide a framework which other countries can use to develop their own assessment methods. However, ways need to be found to streamline the process of standardization and to ensure that published standards influence the work of researchers, environmental regulators and policy makers. Copyright © 2010 John Wiley & Sons, Ltd. [source] First Exposure in Man: Toxicological ConsiderationsBASIC AND CLINICAL PHARMACOLOGY & TOXICOLOGY, Issue 2000Per Spindler Recommendations on the type and extent of preclinical safety studies that should be conducted prior to first dose in man have been developed by the International Conference on Harmonisation, and the European Committee for Proprietary Medicinal Products. These recommendations include studies designed to characterise local tolerance and general toxicity of the drug candidate as well as its genotoxic potential and ability to interfere with reproduction. For trials which can be categorised as low dose PK screening trials and trials with products where rodent and non-rodent (primarily dog) models do not show any biological response (e.g. some biotechnology-derived hormones and cytokines) other testing paradigms should be used. The present recommendations for preclinical testing have had an important impact on the documented impressive safety record of phase I clinical trials. In this spirit we extend our warmest and sincerest thanks to Professor Jens S. Schou for his long and deep engagement in European and International harmonisation of preclinical test recommendations. His efforts have had a substantial impact on the present testing recommendations, which are of obvious benefit to the safety of the patient. [source] European Committee on Antimicrobial Susceptibility Testing (EUCAST) Technical Notes on antimicrobial susceptibility testingCLINICAL MICROBIOLOGY AND INFECTION, Issue 6 2006G. Kahlmeter Abstract The main objectives of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) are to harmonise breakpoints for antimicrobial agents in Europe, and to act as the breakpoint committee for the European Medicines Agency (EMEA) during the registration of new antimicrobial agents. Detailed EUCAST procedures for harmonising and setting breakpoints for antimicrobial agents are available on the EUCAST website. Beginning with the current issue, a series of EUCAST Technical Notes will be published in CMI, based on the rationale documents produced by EUCAST for each of the antimicrobial agents studied, with the aim of highlighting important background information underlying decisions on breakpoints made by EUCAST. [source] Correlation between the procedure for antifungal susceptibility testing for Candida spp. of the European Committee on Antibiotic Susceptibility Testing (EUCAST) and four commercial techniquesCLINICAL MICROBIOLOGY AND INFECTION, Issue 6 2005M. Cuenca-Estrella Abstract The correlation between results obtained with the European Committee on Antibiotic Susceptibility Testing (EUCAST) antifungal susceptibility testing procedure (document 7.1) and four commercial systems was evaluated for a collection of 93 clinical isolates of Candida spp. Overall, agreement between the EUCAST procedure and the Sensititre YeastOne and Etest methods was 75% and 90.4%, respectively. The correlation indices (p < 0.01) between the EUCAST and commercial methods were 0.92 for Sensititre YeastOne, 0.89 for Etest, ,,0.90 for Neo-Sensitabs, and 0.95 for Fungitest. Amphotericin B MICs obtained by Sensititre YeastOne were consistently higher than with the EUCAST method and, although very major errors were not observed, 91% of MICs were misclassified. Amphotericin B- and fluconazole-resistant isolates were identified correctly with Sensititre YeastOne, Etest and Fungitest. Neo-Sensitabs identified amphotericin B-resistant isolates, but misclassified >,5% of fluconazole-resistant isolates as susceptible. The commercial methods, particularly Etest and Fungitest, appeared to be suitable alternatives to the EUCAST procedure for antifungal susceptibility testing of clinical isolates of Candida. [source] Multicenter evaluation of the reproducibility of the proposed antifungal susceptibility testing method for fermentative yeasts of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (AFST-EUCAST)CLINICAL MICROBIOLOGY AND INFECTION, Issue 6 2003M. Cuenca-Estrella Objective To evaluate the intra- and inter-laboratory reproducibility of a new standard for susceptibility testing of fermentative yeasts. This standard is based on the M27-A procedure of the National Committee for Clinical Laboratory Standards (NCCLS), but incorporates several modifications, including spectrophotometric growth-dependent endpoint reading. Methods Nine laboratories participated in the study. Common material lots were used to test six Candida species (one each of C. albicans, C. tropicalis, C. parapsilosis, C. glabrata, C. krusei, and C. lusitaniae), and two quality control strains (C. krusei ATCC6258 and C. parapsilosis ATCC22019). Triplicate testing on three separate days was performed in microtiter format with RPMI,2% glucose, pH 7.0. Flucytosine, fluconazole and itraconazole were tested. In total, 3888 MIC values were included in the analyses. Reproducibility was calculated by means of agreement (percentage of MICs within one two-fold dilution of the mode) and intraclass correlation coefficient (ICC, maximum value of 1). Results The average intra-laboratory agreements were 99% and 96% after 24 h and 48 h of incubation, respectively, with ICCs of 0.98 and 0.97 (P < 0.05). Two strains exhibiting a trailing effect showed intra-laboratory agreement of 92% and ICCs of <,0.91 at 48 h. The inter-laboratory agreement was 94% and 88% after 24 h and 48 h, respectively, with ICCs of 0.93 and 0.91 (P < 0.05). Lower values of agreement and ICCs were obtained for strains exhibiting trailing after 48 h of incubation. Itraconazole yielded the lowest values of reproducibility. Conclusion The new procedure of EUCAST for antifungal susceptibility testing is a reproducible method within and between laboratories and offers several advantages over the NCCLS approved method. [source] | ||||||||||