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End Pieces (end + piece)
Selected AbstractsUltrastructure of spermatozoa of lizards in the genus Mabuya from Central BrazilACTA ZOOLOGICA, Issue 1 2009S. M. De Sá Mandel Abstract This is the first description of spermatozoal ultrastructure of Mabuya skinks. The spermatozoa of the species studied are filiform, consisting of a head region, a midpiece and a tail. The head is characterized by the following features: a depressed acrosome anteriorly, an acrosome vesicle divided into cortex and medulla, paracrystalline subacrosomal material, a pointed tip perforatorium, a circular perforatorium base plate inside the subacrosomal cone, an epinuclear lucent zone separated from the subacrosomal cone by a membrane, a large nuclear rostrum, and round nuclear shoulders. The midpiece presents a bilateral stratified laminar structure, a distal centriole, peripheral fibres 3 and 8 grossly enlarged, columnar mitochondria with linear cristae, dense body rings and a triangular annulus. Finally, the tail is composed of a principal piece and an end piece. An axoneme and a fibrous sheath characterize the principal piece, and the end piece is formed only by an axoneme, which loses its pattern in the last portion. Comparisons with members of Teiidae revealed differences in the numbers of dense rings. A well-developed epinuclear lucent zone in Mabuya is less prominent among teiids. In the spermatozoa of Mabuya, the first ring of dense bodies is very large, uniquely resembling the condition present in snakes. [source] A laser end piece for the treatment of Epistaxis using the Pulsed Dye LaserCLINICAL OTOLARYNGOLOGY, Issue 1 2008J.C.R. Corlett First page of article [source] Aquaporin 11 in the developing mouse submandibular glandEUROPEAN JOURNAL OF ORAL SCIENCES, Issue 1 2010Helga S. Larsen Larsen HS, Ruus A-K, Schreurs O, Kanli Galtung H. Aquaporin 11 in the developing mouse submandibular gland. Eur J Oral Sci 2010; 118: 9,13. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci Several aquaporins (AQPs) have been detected in mature and embryonic mammalian salivary glands (AQP1 and AQP3,AQP8). However, AQP11 has, to our knowledge, never before been described in salivary glands, but is known to be important in, for example, kidney development in mice. We therefore thought it relevant to investigate if AQP11 was present during salivary organogenesis. The submandibular salivary gland (SMG) from CD1 mice was studied during prenatal development and early postnatal development, and also in young adult male and female mice. The expression trend of the AQP11 transcript was detected using the reverse transcription,polymerase chain reaction (RT-PCR), and the temporal,spatial pattern was observed using in situ hybridization. The AQP11 transcript was first detected at embryonic day 13.5 and showed a more or less constitutive expression trend during the prenatal and early postnatal SMG development. Spatial studies demonstrated that the AQP11 transcript was present in the developing and mature duct structures at all stages studied. In the end pieces, the AQP11 transcript was reduced during glandular development. Our results point to an important role for AQP11 during salivary gland development. [source] Design of a Low-Cost Bamboo WellGROUND WATER, Issue 2 2009S.K. Shakya Bamboo wells are an economical alternative for water supply wells in developing countries. The design of the bamboo well described in this article was developed based on field tests. Following field experiments, the screen in the bamboo well was fabricated with seven 3-m-long bamboo strips, each 2 cm wide and 1 cm thick. The strips were bolted on 1-cm-wide mild steel rings spaced 30 cm along the length of the bamboo strips, with 9-cm-long galvanized iron pipe end pieces. Pipes used in the bamboo well were fabricated by wrapping polythene sheets on the bamboo screens. Excellent performance, low cost, and good service life justify the use of a bamboo well for ground water withdrawal in developing countries. [source] Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells.MICROSCOPY RESEARCH AND TECHNIQUE, Issue 4 2010Part 3: Developmental changes in spermatid flagellum, cytoplasmic droplet, egg plasma membrane, interaction of sperm with the zona pellucida Abstract Spermiogenesis constitutes the steps involved in the metamorphosis of spermatids into spermatozoa. It involves modification of several organelles in addition to the formation of several structures including the flagellum and cytoplasmic droplet. The flagellum is composed of a neck region and middle, principal, and end pieces. The axoneme composed of nine outer microtubular doublets circularly arranged to form a cylinder around a central pair of microtubules is present throughout the flagellum. The middle and principal pieces each contain specific components such as the mitochondrial sheath and fibrous sheath, respectively, while outer dense fibers are common to both. A plethora of proteins are constituents of each of these structures, with each playing key roles in functions related to the fertility of spermatozoa. At the end of spermiogenesis, a portion of spermatid cytoplasm remains associated with the released spermatozoa, referred to as the cytoplasmic droplet. The latter has as its main feature Golgi saccules, which appear to modify the plasma membrane of spermatozoa as they move down the epididymal duct and hence may be partly involved in male gamete maturation. The end product of spermatogenesis is highly streamlined and motile spermatozoa having a condensed nucleus equipped with an acrosome. Spermatozoa move through the female reproductive tract and eventually penetrate the zona pellucida and bind to the egg plasma membrane. Many proteins have been implicated in the process of fertilization as well as a plethora of proteins involved in the development of spermatids and sperm, and these are high lighted in this review. Microsc. Res. Tech., 2010. © 2009 Wiley-Liss, Inc. [source] Identification of a heat-shock protein Hsp40, DjB1, as an acrosome- and a tail-associated component in rodent spermatozoaMOLECULAR REPRODUCTION & DEVELOPMENT, Issue 2 2007Masamichi Doiguchi Abstract Iba1 is a 17-kDa EF-hand protein highly expressed in the cytoplasm of elongating spermatids in testis. Using Iba1 as a bait, we performed yeast Two-hybrid screening and isolated a heat-shock protein Hsp40, DjB1, from cDNA library of mouse testis. To characterize DjB1 that is encoded by Dnajb1 gene, we carried out immunoblot analyses, in situ hybridization, and immunohistochemistry. Immunoblot analyses showed that DjB1was constitutively expressed in mouse testis and that its expression level was not changed by heat shock. Dnajb1 mRNA was exclusively expressed in spermatocytes and round spermatids in mouse testis, and Dnajb1 protein DjB1 was predominantly expressed in the cytoplasm of spermatocytes, round spermatids, and elongating spermatids. In mature mouse spermatozoa, DjB1 was localized in the middle and the end pieces of flagella as well as in association with the head (acrosomal region). Association of DjB1 with the acrosomal region in sperm head was also observed in rat spermatozoa. These data suggested that DjB1, which was constitutively expressed in postmeiotic spermatogenic cells in testis, was integrated into spermatozoa as at least two components, that is, sperm head and tail of rodent spermatozoa. Mol. Reprod. Dev. © 2006 Wiley-Liss, Inc. [source] | |||||||||||||