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Elastin-like Polymer (elastin-like + polymer)

Distribution by Scientific Domains
Polymers and Materials Science100%

Selected Abstracts

Development of Biomimetic Chitosan-Based Hydrogels Using an Elastin-Like Polymer,

ADVANCED ENGINEERING MATERIALS, Issue 1-2 2010
Joaquim S. Barbosa
Chitosan and an elastin-like polymer, containing a specific osteoconductive sequence in the primary structure, have been combined to obtain bioactive injectable systems with enhanced mechanical properties and hydrogels. Obtained results indicate that the combination of such polymers may be very promising in the development of biomaterials for minimal invasive orthopaedic reconstructive applications or in bone tissue engineering. The figure shows a thermo-sensitive hydrogel, with a gelation point under physiological temperature. [source]

Responsive Coatings: Stimuli-Responsive Thin Coatings Using Elastin-Like Polymers for Biomedical Applications (Adv. Funct.

ADVANCED FUNCTIONAL MATERIALS, Issue 20 2009
Mater.
Costa et al. successfully apply a simple protein adsorption technique combining chitosan substrates and recombinant elastin-like polymers (ELPs) on page 3210. This process allows for coating of polysaccharide substrates with such biomimetic macromolecules, resulting in interesting properties such as smart behavior and improved cell adhesion. The figure illustrates symbolically the reversible structural changes that occur to the ELP simply by changing temperature. [source]

Stimuli-Responsive Thin Coatings Using Elastin-Like Polymers for Biomedical Applications

ADVANCED FUNCTIONAL MATERIALS, Issue 20 2009
Rui R. Costa
Abstract Smart thin coatings using a recombinant elastin-like polymer (ELP) containing the cell attachment sequence arginine,glycine,(aspartic acid) (RGD) are fabricated for the first time through simple deposition of the ELP dissolved in aqueous-based solutions. The biopolymer is produced and characterized using electrophoresis and mass spectroscopy. The temperature and pH responsiveness are assessed by aggregate size measurements and differential scanning calorimetry. The deposition of the studied ELP onto chitosan is followed in situ with a quartz-crystal microbalance with dissipation monitoring (QCM-D). Contact angle measurements are performed at room temperature and at 50,°C, showing reversible changes from a moderate hydrophobic behavior to an extremely wettable surface. AFM analysis performed at room temperature reveals a smooth surface and no organized structure. At 50,°C, the surface presents spherical nanometer-sized structures of collapsed biopolymer chains. Such results suggest that the ELP chains, when collapsed, aggregate into micelle-like structures at the surface of the substrate, increasing its water affinity. Cell adhesion tests on the developed coatings are conducted using a SaOS-2 cell line. Enhanced cell adhesion could be observed in the H-RGD6-coated surfaces, as compared with the original chitosan monolayer. An intermediate behavior is found in chitosan coated with the corresponding ELP without the RGD sequence. Therefore, the developed films have great potential as biomimetic coatings of biomaterials for different biomedical applications, including tissue engineering and controlled delivery of bioactive agents. Their thermo-responsive behavior can also be exploited for tunable cell adhesion and controlled protein adsorption. [source]

Development of Biomimetic Chitosan-Based Hydrogels Using an Elastin-Like Polymer,

ADVANCED ENGINEERING MATERIALS, Issue 1-2 2010
Joaquim S. Barbosa
Chitosan and an elastin-like polymer, containing a specific osteoconductive sequence in the primary structure, have been combined to obtain bioactive injectable systems with enhanced mechanical properties and hydrogels. Obtained results indicate that the combination of such polymers may be very promising in the development of biomaterials for minimal invasive orthopaedic reconstructive applications or in bone tissue engineering. The figure shows a thermo-sensitive hydrogel, with a gelation point under physiological temperature. [source]

Fabrication of CdSe-Nanofibers with Potential for Biomedical Applications

ADVANCED FUNCTIONAL MATERIALS, Issue 6 2010
Amir Fahmi
Abstract The design and synthesis of nanostructured functional hybrid biomaterials are essential for the next generation of advanced diagnostics and the treatment of disease. A simple route to fabricate semiconductor nanofibers by self-assembled, elastin-like polymer (ELP)-templated semiconductor nanoparticles is reported. Core,shell nanostructures of CdSe nanoparticles with a shell of ELPs are used as building blocks to fabricate functional one-dimensional (1D) nanostructures. The CdSe particles are generated in situ within the ELP matrix at room temperature. The ELP controls the size and the size-distribution of the CdSe nanoparticles in an aqueous medium and simultaneously directs the self-assembly of core,shell building blocks into fibril architectures. It was found that the self-assembly of core,shell building blocks into nanofibers is strongly dependent on the pH value of the medium. Results of cytotoxicity and antiproliferation of the CdSe-ELP nanofibers demonstrate that the CdSe-ELP does not exhibit any toxicity towards B14 cells. Moreover, these are found to be markedly capable of crossing the cell membrane of B14. In contrast, unmodified CdSe nanoparticles with ELPs cause a strong toxic response and reduction in the cell proliferation. This concept is valid for the fabrication of a variety of metallic and semiconductor 1D-architectures. Therefore, it is believed that these could be used not only for biomedical purposes but for application in a wide range of advanced miniaturized devices. [source]

Stimuli-Responsive Thin Coatings Using Elastin-Like Polymers for Biomedical Applications

ADVANCED FUNCTIONAL MATERIALS, Issue 20 2009
Rui R. Costa
Abstract Smart thin coatings using a recombinant elastin-like polymer (ELP) containing the cell attachment sequence arginine,glycine,(aspartic acid) (RGD) are fabricated for the first time through simple deposition of the ELP dissolved in aqueous-based solutions. The biopolymer is produced and characterized using electrophoresis and mass spectroscopy. The temperature and pH responsiveness are assessed by aggregate size measurements and differential scanning calorimetry. The deposition of the studied ELP onto chitosan is followed in situ with a quartz-crystal microbalance with dissipation monitoring (QCM-D). Contact angle measurements are performed at room temperature and at 50,°C, showing reversible changes from a moderate hydrophobic behavior to an extremely wettable surface. AFM analysis performed at room temperature reveals a smooth surface and no organized structure. At 50,°C, the surface presents spherical nanometer-sized structures of collapsed biopolymer chains. Such results suggest that the ELP chains, when collapsed, aggregate into micelle-like structures at the surface of the substrate, increasing its water affinity. Cell adhesion tests on the developed coatings are conducted using a SaOS-2 cell line. Enhanced cell adhesion could be observed in the H-RGD6-coated surfaces, as compared with the original chitosan monolayer. An intermediate behavior is found in chitosan coated with the corresponding ELP without the RGD sequence. Therefore, the developed films have great potential as biomimetic coatings of biomaterials for different biomedical applications, including tissue engineering and controlled delivery of bioactive agents. Their thermo-responsive behavior can also be exploited for tunable cell adhesion and controlled protein adsorption. [source]

Responsive Coatings: Stimuli-Responsive Thin Coatings Using Elastin-Like Polymers for Biomedical Applications (Adv. Funct.

ADVANCED FUNCTIONAL MATERIALS, Issue 20 2009
Mater.
Costa et al. successfully apply a simple protein adsorption technique combining chitosan substrates and recombinant elastin-like polymers (ELPs) on page 3210. This process allows for coating of polysaccharide substrates with such biomimetic macromolecules, resulting in interesting properties such as smart behavior and improved cell adhesion. The figure illustrates symbolically the reversible structural changes that occur to the ELP simply by changing temperature. [source]