			Home About us Contact

Effective Analysis (effective + analysis)

Distribution by Scientific Domains

Chemistry	50%

Selected Abstracts

GIS visualisation and analysis of mobile hydroacoustic fisheries data: a practical example

FISHERIES MANAGEMENT & ECOLOGY, Issue 6 2005
A. R. COLEY
Abstract, Hydroacoustic remote sensing of fish populations residing in large freshwater bodies has become a widely used and effective monitoring tool. However, easy visualisation of the data and effective analysis is more problematic. The use of GIS-based interpolations enables easy visualisation of survey data and an analysis tool for investigating fish populations. Three years of hydroacoustic surveys of Cardiff Bay in South Wales presented an opportunity to develop analysis and visualisation techniques. Inverse distance weighted (IDW) interpolation was used to show the potential of such techniques in analysing survey data both spatially (1-year survey) and temporally (by looking at the spatial changes between years). IDW was fairly successful in visualising the hydroacoustic data for Cardiff Bay. However, other techniques may improve on this initial work and provide improved analysis, total density estimates and statistically derived estimations of prediction error. [source]

On-plate digestion of proteins using novel trypsin-immobilized magnetic nanospheres for MALDI-TOF-MS analysis

PROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 20 2007
Yan Li
Abstract In this study, a novel method of on-plate digestion using trypsin-immobilized magnetic nanospheres was developed followed by MALDI-TOF-MS for rapid and effective analysis and identification of proteins. We utilized a facile one-pot method for the direct preparation of amine-functionalized magnetic nanospheres with highly magnetic properties and the amino groups on the outer surface. Through the reaction of the aldehyde groups with amine groups, trypsin was simply and stably immobilized onto the magnetic nanospheres. The obtained trypsin-linked magnetic nanospheres were then applied for on-plate digestion of sample proteins (myoglobin and Cytochrome c). Moreover, after digestion, the trypsin-linked nanospheres could be easily removed from the plate due to their magnetic property, which would avoid causing contamination on the ion source chamber in MS. The effects of the temperature and incubation time on the digestion efficiency were characterized. Within only 5,min, proteins could be efficiently digested with the peptide sequence coverage higher than or equal to that of the traditional in-solution digestion for 12,h. Furthermore, RPLC fractions of rat liver extract were also successfully processed using this novel method. These results suggested that our improved on-plate digestion protocol for MALDI-MS may find further application in automated analysis of large sets of proteins. [source]

Characterization by mass spectrometry of an unknown polysiloxane sample used under uncontrolled medical conditions for cosmetic surgery

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 21 2008
Cédric Schneider
For a complete understanding of the raw material used for cosmetic surgery under uncontrolled medical conditions, an unknown sample of polydimethylsiloxanes has been investigated utilizing a combination of analytical techniques: pyrolysis/gas chromatography/mass spectrometry (Py/GC/MS), electrospray ionization (ESI)-MS, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)MS, and liquid chromatography (LC)/MS. Among these techniques, the LC/APCI-MS coupling allowed the fastest and more effective analysis. In addition, the complexity of the mass spectra deduced from these LC/MS experiments was simplified compared to the mass spectra obtained by MALDI-TOF. In this work, we have demonstrated how the LC/APCI-MS coupling applied to polydimethylsiloxane samples permits the full characterization of samples where end groups of different nature can be present in very small quantities. Copyright © 2008 John Wiley & Sons, Ltd. [source]

Typologie et vitalité des communautés francophones minoritaires au Canada

THE CANADIAN GEOGRAPHER/LE GEOGRAPHE CANADIEN, Issue 4 2006
ANDRÉ LANGLOIS
La vitalité des communautés francophones minoritaires du Canada, souvent expliquée par les conjonctures démographique et institutionnelle et par le contexte politico-juridique, souffre encore d'une relative rareté de travaux empiriques illustrant l'action de ces facteurs à l'échelle des communautés. Cette rareté n'a pas permis jusqu'à maintenant d'élaborer une véritable analyse de l'effet de milieu sur la vitalité communautaire des minorités francophones. Notre étude se propose de mieux caractériser la présence francophone dans l'espace canadien hors Québec et, ce faisant, les milieux dans lesquels se construit la vie française au pays. Elle se fonde sur l'idée que la présence francophone, caractérisant un lieu donné, profite également d'un contexte plus large dont les ressources ne peuvent faire autrement que de rejaillir sur la communauté locale. À l'idée de présence localisée, nous ajoutons l'idée de présence contextualisée pour mieux faire le lien entre présence et vitalité communautaire. Une méthode originale est présentée ici qui permet de mettre en application cette complémentarité entre ces deux types de présence. Prenant la forme d'une typologie des milieux francophones minoritaires, elle fournit un nouvel éclairage sur la situation des populations francophones du Canada. Une analyse exploratoire des comportements linguistiques de ces populations selon les milieux issus de la typologie montre qu'une telle approche est susceptible d'ouvrir la voie à une meilleure appréhension de la vitalité communautaire en milieu minoritaire. The community vitality of the French-speaking minorities of Canada, often explained by their demographic and institutional situations as well by politico-legal context, still suffers from a relative scarcity of empirical work that illustrates the role of these factors at the community scale. This research neglect has meant that there is no effective analysis of the milieu effect on the community vitality of the French-speaking minorities. This study proposes to better characterize the French-speaking presence in Canada outside Québec and to interpret the milieus upon which French life is built. The analysis is based on the idea that the French-speaking presence that characterizes a given place benefits from a broader context whose resources further impact the local community. To the idea of ,localized presence', we add the idea of ,contextualized presence' to better understand the link between presence and community vitality. An original method is presented which makes it possible to apply the complementarity between these two types of presence. The paper presents a typology of Francophone milieus that provides new insights on the situation of the French-speaking populations of Canada outside Québec. An exploratory analysis of the linguistic behavior of these populations according to the milieus identified within this typology reveals the potential of this analysis for a better understanding of community vitality in a minority situation. [source]