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Efficient Transformation (efficient + transformation)

Distribution by Scientific Domains
Chemistry66%
Life Sciences33%

Selected Abstracts

ChemInform Abstract: Efficient Transformation of Azides to Primary Amines Using the Mild and Easily Accessible CeCl3×7H2O/NaI System.

CHEMINFORM, Issue 26 2008
Giuseppe Bartoli
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a "Full Text" option. The original article is trackable via the "References" option. [source]

PdCl2 -Catalyzed Efficient Transformation of Propargylic Amines to (E)-,-Chloroalkylidene-,-lactams.

CHEMINFORM, Issue 34 2005
Shengming Ma
Abstract For Abstract see ChemInform Abstract in Full Text. [source]

Efficient Transformation of Aldoximes to Nitriles Using 2-Chloro-1-methylpyridinium Iodide under Mild Conditions.

CHEMINFORM, Issue 40 2004
Kieseung Lee
No abstract is available for this article. [source]

Efficient transformation of Lactococcus lactis IL1403 and generation of knock-out mutants by homologous recombination

JOURNAL OF BASIC MICROBIOLOGY, Issue 3 2007
Simon D. Gerber
Abstract Lactococcus lactis IL1403 is a Gram-positive bacterium of great biotechnological interest for food grade applications. Its use is however hampered by the difficulty to efficiently transform this strain. We here describe a detailed, optimized electrotransformation protocol which yields a transformation efficiency of 106 cfu/,g of DNA with the two E. coli Gram-positive shuttle vectors pC3 and pVA838. The utility of the protocol was demonstrated by the generation of single- and double-knock-out mutants by homologous recombination. (© 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source]

Natural transformation of Vibrio fischeri requires tfoX and tfoY

ENVIRONMENTAL MICROBIOLOGY, Issue 8 2010
Amber Pollack-Berti
Summary Recent evidence has indicated that natural genetic transformation occurs in Vibrio cholerae, and that it requires both induction by chitin oligosaccharides, like chitohexaose, and expression of a putative regulatory gene designated tfoX. Using sequence and phylogenetic analyses we have found two tfoX paralogues in all sequenced genomes of the genus Vibrio. Like V. cholerae, when grown in chitohexaose, cells of V. fischeri are able to take up and incorporate exogenous DNA. Chitohexaose-independent transformation by V. fischeri was observed when tfoX was present in multicopy. The second tfoX paralogue, designated tfoY, is also required for efficient transformation in V. fischeri, but is not functionally identical to tfoX. Natural transformation of V. fischeri facilitates rapid transfer of mutations across strains, and provides a highly useful tool for experimental genetic manipulation in this species. The presence of chitin-induced competence in several vibrios highlights the potential for a conserved mechanism of genetic exchange across this family of environmentally important marine bacteria. [source]

First Transformation of Unsaturated Fatty Esters Involving Enyne Cross-Metathesis

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 7-8 2009
Ravalec, Virginie Le
Abstract The ruthenium-catalysed enyne cross-metathesis of several alkyne derivatives with terminal olefins was performed. These reactions were efficiently achieved under mild conditions in dimethyl carbonate, an eco-friendly solvent. A unique one-pot reaction based on an ethenolysis step followed by an enyne cross-metathesis allowed the efficient transformation of renewable unsaturated fatty esters into valuable conjugated 1,3-dienes of interest for further transformations. [source]