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Efficient Extraction (efficient + extraction)
Selected AbstractsEfficient Extraction of Lycopene from Rhodopseudomonas palustris with n -Hexane and Methanol after Alkaline WashCHEMICAL ENGINEERING & TECHNOLOGY (CET), Issue 10 2010Y. Bao Abstract Extraction of lycopene from Rhodopseudomonas palustris with various solvents and alkaline wash was investigated. Dichloromethane or benzene as single polar or nonpolar solvent were the most effective solvents. The maximum extraction efficiency was achieved with a combination of n -hexane and methanol (1:1 v/v). which was approximately one time higher than that obtained with a single solvent. The partitioning behavior of lycopene in n -hexane/methanol indicated that almost all extracted lycopene from R. palustris cells was dissolved in the n -hexane phase. Further studies showed that lycopene extraction was much improved after an alkaline wash of R. palustris cells. The measured lycopene content was much higher than that in tomatoes which indicates that R. palustris will become an important biological resource of lycopene. [source] APPLICATION OF STEPWISE AMMONIUM SULFATE PRECIPITATION AS CLEANUP TOOL FOR AN ENZYME-LINKED IMMUNOSORBENT ASSAY OF GLYPHOSATE OXIDOREDUCTASE IN GENETICALLY MODIFIED RAPE OF GT73JOURNAL OF FOOD BIOCHEMISTRY, Issue 5 2009WENTAO XU ABSTRACT The method of enzyme-linked immunosorbent assay after stepwise ammonium sulfate (AS) purification (AS-ELISA) was developed and used to detect genetically modified (GM) rape of GT73 containing glyphosate oxidoreductase (Gox). Gox protein encoded by the Gox gene from Achromobacter sp. was highly expressed as inclusion bodies in Escherichia coli BL21 (DE3) and purified to homogeneity by Ni2+affinity chromatography. A simple and efficient extraction and purification procedure of Gox protein from the seeds and leaves of GM rape was developed by means of stepwise AS precipitation. Purified polyclonal antibodies against Gox was produced and enzyme-linked immunosorbent assay (ELISA) procedures were established further on to measure the Gox protein. AS-ELISA allowed 5% GMOs to be detected in the seeds of GT73 and 0.5% GMOs to be detected in the leaves of GT73 rape, which makes this method an acceptable method to access Gox protein in GM rape of GT73. PRACTICAL APPLICATIONS Many GMOs containing Gox gene have been approved worldwide such as GT73 rape, 1,445 cotton and Mon832 maize. Protein based methods were more important than DNA based methods, because protein performs a specific and concrete function and is closely interconnected with crop traits. AS-ELISA method can be used in the screening of GM plant, Gox protein expression assay and quantitative detection for GMO labeling. AS-ELISA Gox detecting method was established in this paper and was being evaluated of Inter-laboratory Comparison in some of Chinese GMO detection and assessment centers. With the knowledge of ELISA, ELISA method will be the national standards and international and will be a beneficial supplement for the DNA based GMO detecting methods. [source] Identification and determination of the saikosaponins in Radix bupleuri by accelerated solvent extraction combined with rapid-resolution LC-MSJOURNAL OF SEPARATION SCIENCE, JSS, Issue 13 2010Yun-Yun Yang Abstract A method based on accelerated solvent extraction combined with rapid-resolution LC,MS for efficient extraction, rapid separation, online identification and accurate determination of the saikosaponins (SSs) in Radix bupleuri (RB) was developed. The RB samples were extracted by accelerated solvent extraction using 70% aqueous ethanol v/v as solvent, at a temperature of 120°C and pressure of 100,bar, with 10,min of static extraction time and three extraction cycles. Rapid-resolution LC separation was performed by using a C18 column at gradient elution of water (containing 0.5% formic acid) and acetonitrile, and the major constituents were well separated within 20,min. A TOF-MS and an IT-MS were used for online identification of the major constituents, and 27 SSs were identified or tentatively identified. Five major bioactive SSs (SSa, SSc, SSd, 6,- O -acetyl-SSa and 6,- O -acetyl-SSd) with obvious peak areas and good resolution were chosen as benchmark substances, and a triple quadrupole MS operating in multiple-reaction monitoring mode was used for their quantitative analysis. A total of 16 RB samples from different regions of China were analyzed. The results indicated that the method was rapid, efficient, accurate and suitable for use in the quality control of RB. [source] Aqueous two-phase systems strategies for the recovery and characterization of biological products from plantsJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 9 2010Oscar Aguilar Abstract The increasing interest of the biopharmaceutical industry to exploit plants as economically viable production systems is demanding the development of new downstream strategies to maximize product recovery. Aqueous two-phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological compounds. The present paper gives an overview of the efficient use of ATPS-based strategies for the isolation and partial purification of bioparticles from plant origin. Selected examples highlight the main advantages of this technique, i.e. scaling-up feasibility, process integration capability and biocompatibility. An overview of the recent approach of coupling ATPSs with traditional techniques to increase bioseparation process performance is discussed. A novel approach to characterization protein from plants combining ATPSs and two-dimensional electrophoresis (2-DE) is introduced as a tool for process development. In the particular case of products from plant origin, early success has demonstrated the potential application of ATPS-based strategies to address the major disadvantages of the traditional recovery and purification techniques. This literature review discloses the relevant contribution of ATPSs to facilitate the establishment of bioprocesses in the growing field of high-value products from plants. Copyright © 2010 Society of Chemical Industry [source] | ||||||||||