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Distance Measurements (distance + measurement)
Selected AbstractsHighly Ordered Interstitial Water Observed in Bone by Nuclear Magnetic Resonance,JOURNAL OF BONE AND MINERAL RESEARCH, Issue 4 2005Erin E Wilson Abstract NMR was used to study the nanostructure of bone tissue. Distance measurements show that the first water layer at the surface of the mineral in cortical bone is structured. This water may serve to couple the mineral to the organic matrix and may play a role in deformation. Introduction: The unique mechanical characteristics of bone tissue have not yet been satisfactorily connected to the exact molecular architecture of this complex composite material. Recently developed solid-state nuclear magnetic resonance (NMR) techniques are applied here to the mineral component to provide new structural distance constraints at the subnanometer scale. Materials and Methods: NMR dipolar couplings between structural protons (OH, and H2O) and phosphorus (PO4) or carbon (CO3) were measured using the 2D Lee-Goldburg Cross-Polarization under Magic-Angle Spinning (2D LG-CPMAS) pulse sequence, which simultaneously suppresses the much stronger proton-proton dipolar interactions. The NMR dipolar couplings measured provide accurate distances between atoms, e.g., OH and PO4 in apatites. Excised and powdered femoral cortical bone was used for these experiments. Synthetic carbonate (,2-4 wt%)-substituted hydroxyapatite was also studied for structural comparison. Results: In synthetic apatite, the hydroxide ions are strongly hydrogen bonded to adjacent carbonate or phosphate ions, with hydrogen bond (O-H) distances of ,1.96 Å observed. The bone tissue sample, in contrast, shows little evidence of ordered hydroxide. Instead, a very ordered (structural) layer of water molecules is identified, which hydrates the small bioapatite crystallites through very close arrangements. Water protons are ,2.3-2.55 Å from surface phosphorus atoms. Conclusions: In synthetic carbonated apatite, strong hydrogen bonds were observed between the hydroxide ions and structural phosphate and carbonate units in the apatite crystal lattice. These hydrogen bonding interactions may contribute to the long-range stability of this mineral structure. The biological apatite in cortical bone tissue shows evidence of hydrogen bonding with an ordered surface water layer at the faces of the mineral particles. This structural water layer has been inferred, but direct spectroscopic evidence of this interstitial water is given here. An ordered structural water layer sandwiched between the mineral and the organic collagen fibers may affect the biomechanical properties of this complex composite material. [source] Precision 1H,1H distance measurement via 13C NMR signals: utilization of 1H,1H double-quantum dipolar interactions recoupled under magic angle spinning conditionsMAGNETIC RESONANCE IN CHEMISTRY, Issue 2 2004Yoh Matsuki Abstract We applied the POST-C7 DQ-dipolar recoupling pulse sequence to the measurement of 1H,1H distances with high precision. The spectral resolution is enhanced by detecting the 1H magnetization via 13C signals. A least-squares fitting of the build-up curve of the transferred magnetization to the exact numerical simulations yielded a 1H,,1H, distance of 248 ± 4 pm for fully 13C-labeled L -valine. This distance agrees with the neutron diffraction study. The negative transferred magnetization clearly indicates that the direct DQ 1H,1H dipolar couplings have the largest effect. The signal for the magnetization transfer builds up rapidly by the direct 1H,1H dipolar coupling, and decreases to zero at longer mixing time when the relayed magnetization transfer becomes significant. This large intensity change of the signal leads to the high precision in the distance measurement. We inspected factors that limit the effective bandwidth of the POST-C7 recoupling for the 1H and 13C homonuclear spin systems. The spin interactions at times shorter than the cycle time of the C7 sequence were also evaluated to measure the distances. The carbon-detected 2D 1H DQ mixing experiment was demonstrated for the measurement of multiple 1H,1H distances. Copyright © 2004 John Wiley & Sons, Ltd. [source] Thyromental distance measurement , fingers don't ruleANAESTHESIA, Issue 8 2009P. A. Baker Summary Thyromental distance (TMD) measurement is commonly used to predict difficult intubation. We surveyed anaesthetists to determine how this test was being performed. Comparative accuracy of ruler measurement and other forms of measurement were also assessed in a meta-analysis of published literature. Of respondents, 72% used fingers for TMD measurement and also considered three finger widths the minimum acceptable TMD. In terms of distance, the minimum acceptable TMD was felt to be 6.5 cm by 55% of respondents. However, the actual width of three fingers was (range) 4.6,7.0 cm (mean 5.9 cm), with significant differences between genders and between proximal and distal interphalangeal joints. The meta-analysis showed ruler measurement increased test sensitivity (48% (95% CI 43,53) vs 16% (95% CI 14,19) without a ruler), when predicting difficult intubation. [source] Shape, height, and location of the lingula for sagittal ramus osteotomy in ThaisCLINICAL ANATOMY, Issue 7 2009P. Jansisyanont Abstract This study aims to investigate the shape, height, and location of the lingula in relation to surrounding structures for sagittal ramus osteotomy. Dried Thai mandibles were studied and compared with other races. From both sides of 92 mandibles, the shape of the lingula was classified into triangular, truncated, nodular, or assimilated types. Of 92 mandibles, 146 sides with at least a premolar and a molar on the same side were selected for distance measurement. Height of the lingula was measured from the lingular tip to the mandibular foramen. The location was determined by five distances from the lingular tip to: the anterior and the posterior borders of the mandibular ramus, the mandibular notch, the distal surface of the mandibular second molar, and the occlusal plane. The results showed that truncated lingulae were most frequently found (46.2%) and most appeared to be bilateral (71.7%). Triangular, nodular, and assimilated shapes presented in 29.9%, 19.6%, and 4.3%, respectively. The mean lingular height was 8.2 ± 2.3 mm. The lingula was located at 20.6 ± 3.5 mm from the anterior border of the mandibular ramus and 16.6 ± 2.9 mm from the mandibular notch. In the majority of the mandibles studied, the lingula was located above the occlusal plane. In conclusion, the shape and metric characteristics of the lingula in relation to surrounding structures in Thais vary from other races. All parameters associated with the lingula should be considered for sagittal ramus osteotomy to avoid intraoperative complications. Clin. Anat. 22:787,793, 2009. © 2009 Wiley-Liss, Inc. [source] Organic Ligands Made Porous: Magnetic and Catalytic Properties of Transition Metals Coordinated to the Surfaces of Mesoporous OrganosilicaADVANCED FUNCTIONAL MATERIALS, Issue 7 2010Andreas Kuschel Abstract Inorganic solids with porosity on the mesoscale possess a high internal surface area and a well-accessible pore system. Therefore, it is a relevant task to equip the surfaces of such materials with a maximum density of various organic functional groups. Among these functions it is the capability of coordinating to metal species as a ligand that is of extraordinary importance in many areas, for example, in catalysis. This paper describes how prominent ligands containing donor functions such as carboxylic, thio, chelating, or amine groups can be obtained in the form of nanoporous organosilica materials. The coordination of metal centers such as CoII, MnII, VIV, or PtIV is studied in detail. The magnetic properties of the corresponding materials and some applications in catalysis are reported. A quantitative determination of the surface density of donor atoms by distance measurements using EPR spectroscopy is shown. [source] Genetically Engineered Phage Fibers and Coatings for Antibacterial ApplicationsADVANCED FUNCTIONAL MATERIALS, Issue 2 2010Joan Y. Mao Abstract Multifunctionality can be imparted to protein-based fibers and coatings via either synthetic or biological approaches. Here, potent antimicrobial functionality of genetically engineered, phage-based fibers and fiber coatings, processed at room temperature, is demonstrated. Facile genetic engineering of the M13 virus (bacteriophage) genome leverages the well-known antibacterial properties of silver ions to kill bacteria. Predominant expression of negatively charged glutamic acid (E3) peptides on the pVIII major coat proteins of M13 bacteriophage enables solution-based, electrostatic binding of silver ions and subsequent reduction to metallic silver along the virus length. Antibacterial fibers of micrometer-scale diameters are constructed from such an E3-modified phage via wet-spinning and glutaraldehyde-crosslinking of the E3-modified viruses. Silverization of the free-standing fibers is confirmed via energy dispersive spectroscopy and inductively coupled plasma atomic emission spectroscopy, showing ,0.61,µg cm,1 of silver on E3,Ag fibers. This degree of silverization is threefold greater than that attainable for the unmodified M13,Ag fibers. Conferred bactericidal functionality is determined via live,dead staining and a modified disk-diffusion (Kirby,Bauer) measure of zone of inhibition (ZoI) against Staphylococcus epidermidis and Escherichia coli bacterial strains. Live,dead staining and ZoI distance measurements indicate increased bactericidal activity in the genetically engineered, silverized phage fibers. Coating of Kevlar fibers with silverized E3 phage exhibits antibacterial effects as well, with relatively smaller ZoIs attributable to the lower degree of silver loading attainable in these coatings. Such antimicrobial functionality is amenable to rapid incorporation within fiber-based textiles to reduce risks of infection, biofilm formation, or odor-based detection, with the potential to exploit the additional electronic and thermal conductivity of fully silverized phage fibers and coatings. [source] Counting elephants in Montane forests: some sources of errorAFRICAN JOURNAL OF ECOLOGY, Issue 2 2009Hilde Vanleeuwe Abstract The dung count method is widely used to estimate elephant numbers in forests. It was developed in the lowland forests of Central Africa but it is also used in Montane forests in eastern Africa. Using data collected on Mount Kenya and computer simulations, this paper explores the following issues associated with dung surveys in Montane forests: ,,High rainfall at 3000 m altitude on Mount Kenya was expected to accelerate dung pile decay but no significant difference was found between 3000 and 2500 m where less rain falls, possibly because high rainfall at 3000 m is counteracted by lower temperatures; ,,Physical obstacles make it difficult to walk long, straight transects in Montane forests. Deviating from a straight line pushes the distribution of distance measurements from dung piles to the transect centre line (pdist) towards a negative exponential (NE), which complicates data analysis and may give inaccurate estimates. Using short transects largely alleviate this problem; ,,Analysis of dung count simulations shows that the expected sightability curve of pdist pushes towards a NE with increasing numbers of obstacles blocking the view, even along perfectly straight transects; ,,Extrapolating measured dung density to map area on Mount Kenya resulted in an underestimate of c. 13%. An unstratified correction of map area to ground area for Montane areas would be biased because of the strong tendency for elephants to avoid steeply sloping areas. Résumé La méthode par comptage des crottes est largement utilisée pour estimer le nombre des éléphants en forêt. Elle a été mise au point dans les forêts de basse altitude d'Afrique centrale, mais elle est aussi employée dans les forêts de montagne d'Afrique de l'Est. Utilisant les données collectées sur le Mont Kenya et des simulations informatiques, cet article explore les questions suivantes liées au comptage des crottes dans les forêts de montagne: ,,Les fortes chutes de pluies à 3 000 mètres d'altitude sur le Mont Kenya étaient censées accélérer la décomposition des tas de crottes, mais on n'a pas trouvé de différence significative entre 3 000 et 2 500 mètres où il tombe moins de pluie, peut-être parce que les fortes pluies à 3 000 mètres sont compensées par de plus basses températures; ,,Les obstacles physiques rendent plus difficiles de marcher le long de transects rectilignes dans les forêts de montagne. Le fait de dévier de la ligne droite pousse la distribution des mesures des distances entre les tas de crottes et la ligne droite du transect (pdist) vers un modèle exponentiel négatif (EN) qui complique l'analyse des données et peut donner des estimations inexactes. Le fait de recourir à des transects courts réduit considérablement ce problème; ,,L'analyse des simulations de comptages de crottes montre que la courbe de visibilité attendue de pdist pousse vers un EN lorsque le nombre d'obstacles bloquant la vue augmente, même le long de transects parfaitement rectilignes; ,,L'extrapolation de la densité mesurée des crottes sur une carte du Mont Kenya a abouti à une sous-estimation d'environ 13%. Une correction non stratifiée de la surface cartographiée des zones montagneuses vers une surface plane serait biaisée étant donné que les éléphants ont fortement tendance àéviter les zones escarpées et glissantes. [source] Simple yet stable bearing-only navigationJOURNAL OF FIELD ROBOTICS (FORMERLY JOURNAL OF ROBOTIC SYSTEMS), Issue 5 2010Krajník This article describes a simple monocular navigation system for a mobile robot based on the map-and-replay technique. The presented method is robust and easy to implement and does not require sensor calibration or structured environment, and its computational complexity is independent of the environment size. The method can navigate a robot while sensing only one landmark at a time, making it more robust than other monocular approaches. The aforementioned properties of the method allow even low-cost robots to effectively act in large outdoor and indoor environments with natural landmarks only. The basic idea is to utilize a monocular vision to correct only the robot's heading, leaving distance measurements to the odometry. The heading correction itself can suppress the odometric error and prevent the overall position error from diverging. The influence of a map-based heading estimation and odometric errors on the overall position uncertainty is examined. A claim is stated that for closed polygonal trajectories, the position error of this type of navigation does not diverge. The claim is defended mathematically and experimentally. The method has been experimentally tested in a set of indoor and outdoor experiments, during which the average position errors have been lower than 0.3 m for paths more than 1 km long. © 2010 Wiley Periodicals, Inc. [source] Influence of Artificial Accelerated Aging on Dimensional Stability of Acrylic Resins Submitted to Different Storage ProtocolsJOURNAL OF PROSTHODONTICS, Issue 6 2010Lucas da Fonseca Roberti Garcia DDS Abstract Purpose: The aim of this study was to evaluate the influence of artificial accelerated aging on dimensional stability of two types of acrylic resins (thermally and chemically activated) submitted to different protocols of storage. Materials and Methods: One hundred specimens were made using a Teflon matrix (1.5 cm × 0.5 mm) with four imprint marks, following the lost-wax casting method. The specimens were divided into ten groups, according to the type of acrylic resin, aging procedure, and storage protocol (30 days). GI: acrylic resins thermally activated, aging, storage in artificial saliva for 16 hours, distilled water for 8 hours; GII: thermal, aging, artificial saliva for 16 hours, dry for 8 hours; GIII: thermal, no aging, artificial saliva for 16 hours, distilled water for 8 hours, GIV: thermal, no aging, artificial saliva for 16 hours, dry for 8 hours; GV: acrylic resins chemically activated, aging, artificial saliva for 16 hours, distilled water for 8 hours; GVI: chemical, aging, artificial saliva for 16 hours, dry for 8 hours; GVII: chemical, no aging, artificial saliva for 16 hours, distilled water for 8 hours; GVIII: chemical, no aging, artificial saliva for 16 hours, dry for 8 hours GIX: thermal, dry for 24 hours; and GX: chemical, dry for 24 hours. All specimens were photographed before and after treatment, and the images were evaluated by software (UTHSCSA , Image Tool) that made distance measurements between the marks in the specimens (mm), calculating the dimensional stability. Data were submitted to statistical analysis (two-way ANOVA, Tukey test, p= 0.05). Results: Statistical analysis showed that the specimens submitted to storage in water presented the largest distance between both axes (major and minor), statistically different (p < 0.05) from control groups. Conclusions: All acrylic resins presented dimensional changes, and the artificial accelerated aging and storage period influenced these alterations. [source] Accurate long-range distance measurements in a doubly spin-labeled protein by a four-pulse, double electron,electron resonance methodMAGNETIC RESONANCE IN CHEMISTRY, Issue 12 2008Michela G. Finiguerra Abstract Distance determination in disordered systems by a four-pulse double electron,electron resonance method (DEER or PELDOR) is becoming increasingly popular because long distances (several nanometers) and their distributions can be measured. From the distance distributions eventual heterogeneities and dynamics can be deduced. To make full use of the method, typical distance distributions for structurally well-defined systems are needed. Here, the structurally well-characterized protein azurin is investigated by attaching two (1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl) methanethiosulfonate spin labels (MTSL) by site-directed mutagenesis. Mutations at the surface sites of the protein Q12, K27, and N42 are combined in the double mutants Q12C/K27C and K27C/N42C. A distance of 4.3 nm is found for Q12C/K27C and 4.6 nm for K27C/N42C. For Q12C/K27C the width of the distribution (0.24 nm) is smaller than for the K27C/N42C mutant (0.36 nm). The shapes of the distributions are close to Gaussian. These distance distributions agree well with those derived from a model to determine the maximally accessible conformational space of the spin-label linker. Additionally, the expected distribution for the shorter distance variant Q12C/N42C was modeled. The width is larger than the calculated one for Q12C/K27C by 21%, revealing the effect of the different orientation and shorter distance. The widths and the shapes of the distributions are suited as a reference for two unperturbed MTSL labels at structurally well-defined sites. Copyright © 2008 John Wiley & Sons, Ltd. [source] Advances in insectivore and rodent systematics due to geometric morphometricsMAMMAL REVIEW, Issue 2 2009LENKA BAR ABSTRACT 1Morphometrics, the study of the variation and change in form amongst organisms, serves as a basic methodological tool in various fields of biological research, including systematics. Because it includes information about spatial relationships amongst anatomical landmarks, geometric morphometrics is more suitable for analyzing morphometric variation than methods based on distance measurements. 2Geometric morphometrics allows us to answer general ecological and evolutionary questions about shape. 3In this paper, landmark-based methods are described and illustrated, based on a dataset of measurements from 295 Apodemus mandibles, and the applications of such methods in the systematics of insectivores (Eulipotyphla) and rodents (Rodentia) are summarized. [source] Two measures of the shape of the dark halo of the Milky WayMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2000Rob P. Olling In order to test the reliability of determinations of the shapes of dark-matter haloes of the galaxies, we have made such measurements for the Milky Way by two independent methods. First, we have combined the measurements of the overall mass distribution of the Milky Way derived from its rotation curve and the measurements of the amount of dark matter in the solar neighbourhood obtained from stellar kinematics to determine the flattening of the dark halo. Secondly, we have used the established technique based on the variation in thickness of the Milky Way's H i layer with radius: by assuming that the H i gas is in hydrostatic equilibrium in the gravitational potential of a galaxy, one can use the observed flaring of the gas layer to determine the shape of the dark halo. These techniques are found to produce a consistent estimate for the flattening of the dark-matter halo, with a shortest-to-longest axis ratio of q,0.8, but only if one adopts somewhat non-standard values for the distance to the Galactic centre, R0, and the local Galactic rotation speed, ,0. For consistency, one requires values of R0,7.6 kpc and ,0,190 km s,1. The results depend on the Galactic constants because the adopted values affect both distance measurements within the Milky Way and the shape of the rotation curve, which, in turn, alter the inferred halo shape. Although differing significantly from the current IAU-sanctioned values, these upper limits are consistent with all existing observational constraints. If future measurements confirm these lower values for the Galactic constants, then the validity of the gas-layer-flaring method will be confirmed. Further, dark-matter candidates such as cold molecular gas and massive decaying neutrinos, which predict very flat dark haloes with q,0.2, will be ruled out. Conversely, if the Galactic constants were found to be close to the more conventional values, then there would have to be some systematic error in the methods for measuring dark halo shapes, so the existing modelling techniques would have to be viewed with some scepticism. [source] The proline-rich domain of TonB possesses an extended polyproline II-like conformation of sufficient length to span the periplasm of Gram-negative bacteriaPROTEIN SCIENCE, Issue 4 2010Silvia Domingo Köhler Abstract TonB from Escherichia coli and its homologues are critical for the uptake of siderophores through the outer membrane of Gram-negative bacteria using chemiosmotic energy. When different models for the mechanism of TonB mediated energy transfer from the inner to the outer membrane are discussed, one of the key questions is whether TonB spans the periplasm. In this article, we use long range distance measurements by spin-label pulsed EPR (Double Electron,Electron Resonance, DEER) and CD spectroscopy to show that the proline-rich segment of TonB exists in a PPII-like conformation. The result implies that the proline-rich segment of TonB possesses a length of more than 15 nm, sufficient to span the periplasm of Gram-negative bacteria. [source] | ||||||||||||||||