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Display
Kinds of Display Terms modified by Display Selected AbstractsEVOLUTION OF COLORFUL DISPLAYEVOLUTION, Issue 3 2007Gerald Borgia How the displays of bowerbirds have evolved has attracted widespread interest. Endler et al. (2005) analyzed color use in display in a subset of bowerbird species and generalized their results to all bowerbirds. Here we discuss problems with their analysis that calls into question their conclusions. For example, they state that bowerbirds do not use decorations that match their background, but this is not supported by their results. They reconstruct historical patterns of sexual dimorphism in plumage display using questionable methodology. The high lability of these display traits makes these reconstructions unreliable and, using accepted methods and acknowledging the lability problem, we were unable to support their conclusions. Their claim that plumage differences between sympatric species are due to character displacement is not supported by the available data. Their focus is on visual contrast as the cause for display color and we offer additional hypotheses that may contribute to explaining color use. We support studies of spectral analysis of display traits but urge greater care in using this information to reach conclusions about how colorful displays have evolved. [source] COSTS OF AN INDUCED IMMUNE RESPONSE ON SEXUAL DISPLAY AND LONGEVITY IN FIELD CRICKETSEVOLUTION, Issue 10 2004Alain Jacot Abstract Immune system activation may benefit hosts by generating resistance to parasites. However, natural resources are usually limited, causing a trade-off between the investment in immunity and that in other life-history or sexually selected traits. Despite its importance for the evolution of host defense, state-dependent fitness costs of immunity received little attention under natural conditions. In a field experiment we manipulated the nutritional condition of male field crickets Gryllus campestris and subsequently investigated the effect of an induced immune response through inoculation of bacterial lipopolysaccharides. Immune system activation caused a condition-dependent reduction in body condition, which was proportional to the condition-gain during the preceding food-supplementation period. Independent of nutritional condition, the immune insult induced an enduring reduction in daily calling rate, whereas control-injected males fully regained their baseline level of sexual signaling following a temporary decline. Since daily calling rate affects female mate choice under natural conditions, this suggests a decline in male mating success as a cost of induced immunity. Food supplementation enhanced male life span, whereas the immune insult reduced longevity, independent of nutritional status. Thus, immune system activation ultimately curtails male fitness due to a combined decline in sexual display and life span. Our field study thus indicates a key role for fitness costs of induced immunity in the evolution of host defense. In particular, costs expressed in sexually selected traits might warrant the honest advertisement of male health status, thus representing an important mechanism in parasite-mediated sexual selection. [source] MORTUARY DISPLAY AND CULTURAL CONTACT: A CEMETERY AT KASTRI ON THASOSOXFORD JOURNAL OF ARCHAEOLOGY, Issue 4 2006SARA OWEN Summary. This article explores how the patterns of development within the local populations prior to Greek colonization, or even Greek contact, can elucidate the process of Greek colonization. Focusing upon the Thracian Early Iron Age cemetery of Kastri on Thasos, it suggests that past interpretations of such cemeteries as undifferentiated is due to the imposition of modern ideas of value. This article instead uses the criterion of diversity to suggest that the cemetery in fact has clear patterns of social differentiation in the first and last periods of use. Furthermore imports are restricted to graves of highest diversity in the last period of use (the early seventh century BC). This pattern is repeated over Early Iron Age Thrace, and is indicative of a social change within Thrace prior to Greek colonization which saw nascent Thracian elites seeking out imports from many areas in order to bolster their status. [source] THE DISPLAY OF THE DEAD ON THE GREEK TRAGIC STAGE: THE CASE OF EURIPIDES, SUPPLICES,BULLETIN OF THE INSTITUTE OF CLASSICAL STUDIES, Issue 1 2008ELENI KORNAROU First page of article [source] COLORFUL THOUGHTS ABOUT COLORFUL DISPLAYSEVOLUTION, Issue 3 2007John A. Endler No abstract is available for this article. [source] WARNING DISPLAYS IN SPINY ANIMALS: ONE (MORE) EVOLUTIONARY ROUTE TO APOSEMATISMEVOLUTION, Issue 12 2005Michael P. Speed Abstract To date, theoretical or laboratory simulations of aposematic evolution in prey animals have focused narrowly on internally stored chemical defense as the source of unprofitability and ignore aposematic advertisement of physical defenses such as spines (and defensive hairs, claws, etc.). This has occurred even though aposematism in spiny animals has been recognized since the 19th century. In this paper we present the first detailed theoretical consideration of aposematism in spiny animals, focusing on questions of initial evolution, costs of display, and coevolution of displays with defenses. Using an individual-based evolutionary model, we found that spines (or similar physical defenses) can easily evolve without aposematism, but when spines do evolve, aposematic displays can also easily evolve if they help to make the prey animal distinctive and if they draw attention to the physical threat. When aposematic displays evolve, they cause reduced investment in costly spines, so that, in addition to signaling unprofitability, aposematic display may enhance the cost-effectiveness of antipredator defenses (one exception to this conclusion is if the display is itself as costly as the defense). For animals with stinging spines, combining physical and chemical defense, the evolution of aposematic display may lead to reduced investment in the toxin compared to the spine. This occurs because spines act as both secondary (repellent) defenses and as primary defenses (their own visible, honest advertisement), whereas internally stored toxins only (generally) act as repellent secondary defenses. We argue that conspicuous aposematism in spines functions as an attention-getting mechanism, whereas conspicuous aposematic display in purely toxic animals may be explained by signal reliability arguments. Finally, one (more) route by which aposematism may initially evolve is by spiny rather than purely chemically defended species, spreading to species with other forms of secondary defense as the signal becomes common. [source] Slavery, Memory, and Museum Display in Baltimore: The Great Blacks in Wax and the Reginald F. LewisCURATOR THE MUSEUM JOURNAL, Issue 2 2009Marcus Wood The analysis deals with the question by focusing on the radically contrasting museological, aesthetic, and ethical codes of the Great Blacks in Wax Museum, and the Reginald Lewis Museum, both situated in Baltimore, Maryland. Three key sites are isolated for discussion: the names of the museums, their approaches to the topic of the Middle Passage, and lynching. While both museums have made important cultural contributions to the public memorialization of highly charged subjects, the Great Blacks in Wax emerges as the more radical institution, closely in touch with the dynamic and creative museum aesthetic of the wider Black Atlantic Diaspora, and of Brazil in particular. [source] Chemoselective Reagents for Covalent Capture and Display of Glycans in Microarrays,EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, Issue 3 2010Emiliano Cló Abstract Glycobiology has made very significant progress in the past decades. However, further progress will significantly depend on the establishment of novel methods for miniaturized, high-throughput analysis of glycan,protein interactions. Robust solid-phase chemical tools and new, chemoselective reagents for biologically meaningful display of surface-immobilized glycans are likely to play a key role. Here we present four new bifunctional linkers that allow highly chemoselective capture of unprotected glycans in solution to form glycan-linker conjugates for direct construction of glycan microarrays (glycochips). The bifunctional linkers carry O -linked aminooxy moieties, some with N -substituents at one end and an amino group at the other. In addition, they contain a substituted benzene ring for UV traceability and improved purification of glycan-linker conjugates. NMR spectroscopic studies in solution proved that N -substituted aminooxy linkers provided model glycan-linker conjugates with the ,-glucopyranoside configuration, i.e. the ring-closed form required for biological recognition. Then an ensemble of glycan-linker conjugates were assembled from mannobiose, lactose, and N -acetyl-lactosamine and used for covalent printing of glycan microarrays. The stability of oximes were studied both in solution and on-chip. In solution, two of the linkers provided glycan-linker conjugates with a remarkable stability at pH 4 or higher, on-chip this relative stability was upheld. Two of the linkers, with different properties, are recommended for the glycobiology toolbox for the construction of glycan microarrays from unprotected glycans. [source] Encapsulated-Dye All-Organic Charged Colored Ink Nanoparticles for Electrophoretic Image DisplayADVANCED MATERIALS, Issue 48 2009Sun Wha Oh Electrophoretic ink nanoparticles with high mobility are successfully fabricated by dispersion polymerization. The color of test cells can be changed by applying a bias voltage, as shown in the figure: the lower row shows the same cells as the upper row but with an applied voltage. These all-organic, encapsulated-dye, electrophoretic ink particles are expected to reduce the fabrication cost of e-ink in electrophoretic image display cells. [source] Fast High-Temperature Response of Carbon Nanotube Film and Its Application as an Incandescent DisplayADVANCED MATERIALS, Issue 35 2009Peng Liu Super aligned carbon nanotube (CNT) film shows a fast high-temperature response: the film can be heated to incandescence and cools down in about 1 ms. Using screen printing and laser cutting, an incandescent CNT film array that can dynamically display Chinese characters is fabricated. More applications of the film may be developed based on its fast response. [source] Ribosome Display and Dip-Pen Nanolithography for the Fabrication of Protein Nanoarrays,ADVANCED MATERIALS, Issue 17 2008Jung Dong Kim Protein nanochip fabrication is performed via immobilizing proteins by dip-pen nanolithography (DPN) and the ribosome display technique. Protein,ribosome,mRNA fusion molecules permit the simultaneous immobilization of functional proteins without purification through hybridization to complementary DNA that has been immobilized on a nanometer scale on the surface via DPN. [source] Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display,ADVANCED MATERIALS, Issue 10 2008Marc D. Roy A peptide sequence SVSVGMKPSPRP that selectively recognizes hydroxyapatite (HA) is identified by using a phage display approach. The engineered sequence exhibits chemical and structural specificity for HA over calcium carbonate and HA's amorphous calcium phosphate precursor. In situ binding to HA in a tooth cross section further demonstrates the sequence specificity and utility in nondestructive imaging applications. [source] Use of a novel technology for presenting screening measures to detect mild cognitive impairment in elderly patientsINTERNATIONAL JOURNAL OF CLINICAL PRACTICE, Issue 9 2010D. W. Wright Summary Background:, Available screening tools for mild cognitive impairment (MCI), often a precursor to Alzheimer's disease, are insensitive or not feasible for administration in a busy primary care setting. Display Enhanced TEsting for Cognitive impairment and Traumatic brain injury (DETECTÔ) addresses these issues by creating an immersive environment for the brief administration of neuropsychological (NP) measures. Objective:, The aim of this study was to determine if the DETECTÔ cognitive subtests can identify MCI patients as accurately as standard pen and paper NP tests. Methods:, Twenty patients with MCI recruited from a memory disorders clinic and 20 age-matched controls were given both a full battery of NP tests (standard NP) and the DETECTÔ screen. Logistic regression models were used to determine whether individual tests were predictive of group membership (MCI or control). Demographic variables including age, race, education and gender were adjusted as covariates. Selection methods were used to identify subset models that exhibited maximum discrimination between MCI patients and controls for both testing methods. Results:, Both the standard NP model (C-index = 0.836) and the DETECTÔ model (C-index = 0.865) showed very good discrimination and were not significantly different (p = 0.7323). Conclusion:, The DETECTÔ system shows good agreement with standard NP tests and is capable of identifying elderly patients with cognitive impairment. [source] Museum Skepticism: A History of the Display of Art in Public Galleries by carrier, davidJOURNAL OF AESTHETICS AND ART CRITICISM, Issue 3 2007JEFFREY WILSON No abstract is available for this article. [source] The City as Social Display: Landed Elites and Urban Images in Charleston and PalermoJOURNAL OF HISTORICAL SOCIOLOGY, Issue 4 2001Enrico Del Lago The landed elites of Charleston and Palermo successfully modified the layout of the two cities by choosing particular areas of residence in which they could express their economic and social exclusivity through ,representational' architecture. In doing this, the two landed elites constructed images of the cities which built upon already established ones acquired in previous centuries. While the old images were the symbolic expressions of the political domination of two distant states over their colonies, the new images symbolized the power of the landed aristocrats, their domination of the social and economic life of the cities, and their commitment to nationalist struggles against new and hostile political institutions. [source] Microvascular Display of Xanthine Oxidase and NADPH Oxidase in the Spontaneously Hypertensive RatMICROCIRCULATION, Issue 7 2006FRANK A. DELANO ABSTRACT Objective: Oxygen free radical production in hypertension may be associated with elevated arteriolar tone and organ injury. Previous results suggest an enhanced level of oxygen free radical formation in microvascular endothelium and in circulating neutrophils associated with xanthine oxidase activity in the spontaneously hypertensive rats (SHR) compared with their normotensive controls, the Wistar Kyoto rats (WKY). The aim of this study was to gain more detailed understanding of where oxidative enzymes are located in the microcirculation. Methods: An approach was developed to delineate the cellular distribution of two selected oxidative enzymes, xanthine oxidase and nicotinamide adenine dinucleotide phosphate (NADPH) dependent oxidase (protein 67-kDa fraction). Immunolabeling with peroxidase substrate was utilized, which permits full delineation of the primary antibody in all microvascular structures of the mesentery. Results: Xanthine oxidase is present in the endothelium of all segments of the microcirculation, in mast cells, and in parenchymal cells of the mesentery. NADPH oxidase can be detected in the endothelium, leukocytes, and mast cells and with lower levels in parenchymal cells. The mesentery of WKY and SHR has similar enzyme distributions with enhancements on the arteriolar and venular side of the microcirculation that coincide with the sites of enhanced free radical production recently reported. Immune label measurements under standardized conditions indicate that both enzymes are significantly enhanced in the SHR. Adrenalectomy, which serves to reduce the blood pressure and free radical production of the SHR to normotensive levels, leads to a reduction of NADPH and xanthine oxidase to normotensive levels, while supplementation of adrenalectomized SHR with dexamethasone significantly increases the oxidase expression in several parts of the microcirculation to levels above the WKY rats. Conclusion: The results indicate that enhanced expression of NADPH and xanthine oxidase in the SHR depends on an adrenal pathway that is detectable in the arteriolar and venular network at high and low pressure regions of the circulation. [source] The Politics of Display or the Display of Politics?MUSEUM ANTHROPOLOGY, Issue 2 2002Cultural Policy, the Museo del Hombre Dominicano [source] Strategic Display and Response to Emotions: Developing Evidence-based Negotiation Expertise in Emotion Management (NEEM)NEGOTIATION AND CONFLICT MANAGEMENT RESEARCH, Issue 4 2008Georges Potworowski Abstract This article conceptualizes emotion management as a form of negotiation expertise, and integrates the nascent empirical literature on emotion in negotiation with concepts from the learning sciences literature to suggest how negotiation expertise in emotion management (NEEM) can be taught. We argue that NEEM differs from emotional intelligence in fundamental ways, and that it consists of sensitivity to strategically relevant emotional cues, ability to strategically display and respond to emotions in negotiations, and the inclination to manage emotions for superior objective and subjective negotiation performance. We propose a method of developing NEEM in the classroom and identify directions for future research. [source] A High-Speed Passive-Matrix Electrochromic Display Using a Mesoporous TiO2 Electrode with Vertical PorosityANGEWANDTE CHEMIE, Issue 23 2010Wu Weng Klar und deutlich: Ein leistungsstarkes und schnelles elektrochromes Display (ECD) mit passiver Matrix wurde aus einem Leukofarbstoff und einer nanoporösen TiO2 -Elektrode konstruiert (siehe Bild). Die senkrechten Poren verhindern eine Bewegung der Farbstoffmoleküle und führen dadurch zu klaren Bildern bei hohen Frequenzen. Mit einer farbigen Variante dieses ECD könnten konkurrenzfähige elektrische Reflektivdisplays entwickelt werden. [source] A Population of Thermostable Reverse Transcriptases Evolved from Thermus aquaticus DNA Polymerase,I by Phage Display,ANGEWANDTE CHEMIE, Issue 7 2010Sophie Vichier-Guerre Dr. No abstract is available for this article. [source] Display of a peer's face picture enhances the preference for a pen in preference testing in cowsANIMAL SCIENCE JOURNAL, Issue 5 2009Shigeru NINOMIYA ABSTRACT Using preference testing, we investigated the effect of a brush and a peer's face picture on the welfare of breeding beef cows. Four cows were used in experiment 1 and another four cows were used in experiment 2. An experimental barn consisted of two 3.5 square-meter pens, and a corridor measuring 1.6 m wide and 1.3 m long. During the experiment, either side of the pens were treated. In experiment 1, straw bedding was placed in a treated pen (condition B) in the first two trials, a brush was added in the treated pen (condition BB) in the next two trials, and a peer's face picture was displayed in the treated pen (condition BBF) in the last two trials. In experiment 2, condition B was applied for the first two trials, a peer's face picture was displayed in the treated pen (condition BF) in the next two trials, and condition BBF was applied for the last two trials. Each cow was housed alone from 09.00 hour to 15.00 h in the experimental barn during the six trials. The cows stayed in the treatment pen longer than in the non-treatment pen under all conditions in experiments 1 and 2. In experiment 1, the mean total time spent in the treatment pen was longer under condition BBF than under conditions B and BB. In experiment 2, the mean total time spent in the treatment pen was shorter under condition B than under conditions BF and BBF. The mean percentage of time spent ruminating was greater under condition BBF than under condition B. It is concluded that the cows preferred the peer's face picture in the isolated condition of preference testing in this study. [source] Dynamic Display, Propaganda, and the Reinforcement of Provincial Power in the Inca EmpireARCHEOLOGICAL PAPERS OF THE AMERICAN ANTHROPOLOGICAL ASSOCIATION, Issue 1 2004Dennis Ogburn A primary objective of the Inca state and other early empires was to maintain control over the inhabitants of conquered territories. In addition to overt tactics such as military force, resettlement, alliance formation, and cultural and economic integration, I suggest that many other activities of the Inca state also served to reinforce state power in the provinces. This was achieved by establishing and maintaining a "psychology of submission" in subject peoples through frequent reminders of imperial power, that is, advertising state control over labor. Display in this form was a major implicit element in various activities of the empire, such as the movement of armies, the transport of building stones from Cuzco to Ecuador, and the construction of imperial temples and palaces. These activities served as potent public demonstrations of the state's ability to mobilize large armies and control enormous amounts of labor. Propaganda also played a major role in maintaining the psychology of submission by disseminating information about those activities having an element of display and about other accomplishments of the state to people who did not witness them firsthand. Considered in light of these mechanisms of display and propaganda, many of the apparently wasteful activities of the Inca state can be understood as deliberate and integral to overall imperial strategies for maintaining control in the provinces. [source] Post-translational regulation of expression and conformation of an immunoglobulin domain in yeast surface displayBIOTECHNOLOGY & BIOENGINEERING, Issue 1 2006Ranganath Parthasarathy Abstract Display of heterologous proteins on the surface of Saccharomyces cerevisiae is increasingly being exploited for directed evolution because of straightforward cell screens. However, yeast post-translationally modifies proteins in ways that must be factored into library engineering and refinement. Here, we express the extracellular immunoglobulin domain of an ubiquitous mammalian membrane protein, CD47, which is implicated in cancer, immunocompatibility, and motility. CD47 has multiple sites of glycosylation and a core disulfide bond. We assess the effects of both of these post-translational modifications on expression and antibody binding. CD47's extracellular domain is fused to the yeast mating protein Aga2p on the cell wall, and the resulting fusion protein binds several key antibodies, including a conformation-sensitive antibody. Site-by-site mutagenesis of CD47's five N-linked glycosylation sites progressively decreases expression levels on yeast, but folding appears stable. Cysteine mutations disrupt the expected core disulfide, and also decrease protein expression levels, though not to the extent seen with complete deglycosylation. However, with the core disulfide mutants, antibody binding proves to be lower than expected from expression levels and glycosylation is clearly reduced compared to wild-type. The results indicate that glycosylation regulates heterologous display on yeast more than core disulfides do and thus suggest bounds on directed evolution by post-translational processing. © 2005 Wiley Periodicals, Inc. [source] Development of a vaccine marker technology: Display of B cell epitopes on the surface of recombinant polyomavirus-like pentamers and capsoids induces peptide-specific antibodies in piglets after vaccinationBIOTECHNOLOGY JOURNAL, Issue 12 2006Markus Neugebauer Abstract Highly immunogenic capsomers (pentamers) and virus-like particles (VLPs) were generated through insertion of foreign B cell epitopes into the surface-exposed loops of the VP1 protein of murine polyomavirus and via heterologous expression of the recombinant fusion proteins in E. coli. Usually, complex proteins like the keyhole limpet hemocyanin (KLH) act as standard carrier devices for the display of such immunogenic peptides after chemical linkage. Here, a comparative analysis revealed that antibody responses raised against the carrier entities, KLH or VP1 pentamers, did not significantly differ up to 18 weeks, demonstrating the highly immunogenic nature of VP1-based particulate structures. The carrier-specific antibody response was reproducibly detected in the meat juice after processing. More importantly, chimeric VP1 pentamers and VLPs carrying peptides of 12 and 14 amino acids in length, inserted into the BC2 loop, induced a strong and long-lasting humoral immune response against VP1 and the inserted foreign epitope. Remarkably, the epitope-specific antibody response was only moderately decreased when VP1 pentamers were used instead of VLPs. In conclusion, we identified polyomavirus VP1-based structures displaying surface-exposed immunodominant B cell epitopes as being an efficient carrier system for the induction of potent peptide-specific antibodies. The application of this approach in vaccine marker technology in livestock holding and the meat production chain is discussed. [source] Physiological Improvement to Enhance Escherichia coli Cell-Surface Display via Reducing Extracytoplasmic StressBIOTECHNOLOGY PROGRESS, Issue 2 2008Niju Narayanan Cell physiology was impaired when enhanced yellow fluorescence protein (EYFP) was displayed on the Escherichia coli cell surface, resulting in growth arrest and poor display performance. Coexpression of Skp, a periplasmic chaperone known to interact with several outer membrane proteins for their transport and insertion in the outer membrane, was demonstrated to be effective to restore cell physiology. When Skp was coexpressed with EYFP display, host cells became less sensitive to ethylenediaminetetraacetic acid and sodium dodecyl sulfate, implying that cell physiology was improved. Most importantly, the display performance was highly enhanced as a result of the increased specific fluorescence intensity without growth arrest. The results of transmission electron microscopy indicate that the density of surface-displayed EYFP was highly increased upon Skp coexpression. Cells with EYFP display experienced extracytoplasmic stress, as reflected by the induced promoter activities of three stress-responsive genes, degP, cpxP, and rpoH. The extracytoplasmic stress reflected by the degP promoter activity appears to be consistent with the cell physiology observed phenotypically under various culture conditions for cell-surface display. Therefore, the PdegP:: lacZ allele was proposed to be a suitable "sensor" for monitoring the extracytoplasmic stress and cell physiology during the course of E. coli cell-surface display. [source] Bipartite Tetracysteine Display Requires Site Flexibility for ReAsH CoordinationCHEMBIOCHEM, Issue 10 2009Jessica L. Goodman Dr. Abstract Flexibility required: We designed intramolecular bipartite tetracysteine sites in loops of p53 and the ,-sheets of EmGFP. We found that ReAsH binding preferentially favors tetracysteine sites with flexible geometries such as loops; flexibility was assessed by comparing C, B-factor values. This information is important for directing successful bipartite tetracysteine site designs. [source] Design of Cell-Surface-Retained Polymers for Artificial Ligand DisplayCHEMBIOCHEM, Issue 2 2009Ryosuke Kamitani We propose a cell-surface modification method based on the use of synthetic polymers that can be retained on the cell surface without rapid internalization. Secondary amine-displaying cationic polymers act as an effective scaffold for the display of artificial ligands, thus affording a new technique for the control of cell adhesion events through the specific recognition of these ligands. [source] Selection of D -Amino-Acid Peptides That Bind to Alzheimer's Disease Amyloid Peptide A,1,42 by Mirror Image Phage DisplayCHEMBIOCHEM, Issue 8 2003Katja Wiesehan Dr. Abstract A mirror image phage display approach was used to identify novel and highly specific ligands for Alzheimer's disease amyloid peptide A,(1,42). A randomized 12-mer peptide library presented on M13 phages was screened for peptides with binding affinity for the mirror image of A,(1,42). After four rounds of selection and amplification the peptides were enriched with a dominating consensus sequence. The mirror image of the most representative peptide (D -pep) was shown to bind A,(1,42) with a dissociation constant in the submicromolar range. Furthermore, in brain tissue sections derived from patients that suffered from Alzheimer's disease, amyloid plaques and leptomeningeal vessels containing A, amyloid were stained specifically with a fluorescence-labeled derivative of D -pep. Fibrillar deposits derived from other amyloidosis were not labeled by D -pep. Possible applications of this novel and highly specific A, ligand in diagnosis and therapy of Alzheimer's disease are discussed. [source] ChemInform Abstract: Dinuclear {(salen)Al} Complexes Display Expanded Scope in the Conjugate Cyanation of ,,,-Unsaturated Imides.CHEMINFORM, Issue 27 2008Clement Mazet Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a "Full Text" option. The original article is trackable via the "References" option. [source] Glycosyltransferase Microarray Displayed on the Glycolipid LB MembraneADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 6-7 2003Noriko Nagahori Abstract ,(1,4),Galactosyltransferase expressed as a fusion protein with maltose binding protein (MBP-GalT) was displayed specifically on a Langmuir,Blodgett (LB) membrane prepared by photopolymerization of maltotriose-carrying glycolipid (1) with 1,2-bis(10,12-tricosadiynoyl)- sn -glycero-3-phosphocholine (2). The catalytic activity of MBP-GalT on the LB film was directly monitored by the surface plasmon resonance (SPR) method using a GlcNAc-carrying water-soluble polymer (3) as an acceptor substrate. Highly sensitive sigmoidal-type signals were obtained upon the addition of the acceptor substrate in the presence of the donor substrate, UDP-galactose (UDP-Gal), while the binding of 3 was not detected in the absence of UDP-Gal. The intensities of the signals were dependent on the amount of immobilized MBP-GalT on the LB film, which was estimated from the images obtained by atomic force microscope (AFM). [source] | |||||||||||||||||||||||||||||||||||||||||||||||||||||||