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Differential Stability (differential + stability)

Distribution by Scientific Domains
Chemistry40%

Selected Abstracts

Personality Development From Late Adolescence to Young Adulthood: Differential Stability, Normative Maturity, and Evidence for the Maturity-Stability Hypothesis

JOURNAL OF PERSONALITY, Issue 2 2007
M. Brent Donnellan
ABSTRACT This investigation examined personality development during the transition from adolescence to adulthood using the brief form of the Multidimensional Personality Questionnaire (Patrick, Curtin, & Tellegen, 2002). Parent and self-reports of personality were obtained in 1994 (average age=17.60 years), and self-reports were obtained in 2003 (average age=27.24 years). There was evidence of both differential stability and normative changes in the direction of increased functional maturity during this transition. Moreover, adolescents with more mature personalities in 1994 tended to show fewer personality changes from 1994 to 2003. These maturity-stability effects held when parent reports were used to assess personality. All told, there was evidence of both stability and change in personality during the transition to adulthood. [source]

Structure of the heterotrimeric PCNA from Sulfolobus solfataricus

ACTA CRYSTALLOGRAPHICA SECTION F (ELECTRONIC), Issue 10 2006
Gareth J. Williams
PCNA is a ring-shaped protein that encircles DNA, providing a platform for the association of a wide variety of DNA-processing enzymes that utilize the PCNA sliding clamp to maintain proximity to their DNA substrates. PCNA is a homotrimer in eukaryotes, but a heterotrimer in crenarchaea such as Sulfolobus solfataricus. The three proteins are SsoPCNA1 (249 residues), SsoPCNA2 (245 residues) and SsoPCNA3 (259 residues). The heterotrimeric protein crystallizes in space group P21, with unit-cell parameters a = 44.8, b = 78.8, c = 125.6,Å, , = 100.5°. The crystal structure of this heterotrimeric PCNA molecule has been solved using molecular replacement. The resulting structure to 2.3,Å sheds light on the differential stabilities of the interactions observed between the three subunits and the specificity of individual subunits for partner proteins. [source]

Personality Development From Late Adolescence to Young Adulthood: Differential Stability, Normative Maturity, and Evidence for the Maturity-Stability Hypothesis

JOURNAL OF PERSONALITY, Issue 2 2007
M. Brent Donnellan
ABSTRACT This investigation examined personality development during the transition from adolescence to adulthood using the brief form of the Multidimensional Personality Questionnaire (Patrick, Curtin, & Tellegen, 2002). Parent and self-reports of personality were obtained in 1994 (average age=17.60 years), and self-reports were obtained in 2003 (average age=27.24 years). There was evidence of both differential stability and normative changes in the direction of increased functional maturity during this transition. Moreover, adolescents with more mature personalities in 1994 tended to show fewer personality changes from 1994 to 2003. These maturity-stability effects held when parent reports were used to assess personality. All told, there was evidence of both stability and change in personality during the transition to adulthood. [source]

N-terminal extension changes the folding mechanism of the FK506-binding protein

PROTEIN SCIENCE, Issue 9 2001
Alla Korepanova
Abstract Many of the protein fusion systems used to enhance the yield of recombinant proteins result in the addition of a small number of amino acid residues onto the desired protein. Here, we investigate the effect of short (three amino acid) N-terminal extensions on the equilibrium denaturation and kinetic folding and unfolding reactions of the FK506-binding protein (FKBP) and compare the results obtained with data collected on an FKBP variant lacking this extension. Isothermal equilibrium denaturation experiments demonstrated that the N-terminal extension had a slight destabilizing effect. NMR investigations showed that the N-terminal extension slightly perturbed the protein structure near the site of the extension, with lesser effects being propagated into the single ,-helix of FKBP. These structural perturbations probably account for the differential stability. In contrast to the relatively minor equilibrium effects, the N-terminal extension generated a kinetic-folding intermediate that is not observed in the shorter construct. Kinetic experiments performed on a construct with a different amino acid sequence in the extension showed that the length and the sequence of the extension both contribute to the observed equilibrium and kinetic effects. These results point to an important role for the N terminus in the folding of FKBP and suggest that a biological consequence of N-terminal methionine removal observed in many eukaryotic and prokaryotic proteins is to increase the folding efficiency of the polypeptide chain. [source]

The nuclear gene HCF107 encodes a membrane-associated R-TPR (RNA tetratricopeptide repeat)-containing protein involved in expression of the plastidial psbH gene in Arabidopsis

THE PLANT JOURNAL, Issue 5 2005
Aniruddha P. Sane
Summary Expression of the genes of plastidial psbB operon (psbB-psbT-psbH-petB-petD) involves multiple processing events and formation of several mono-, di- and multi-cistronic transcripts which are further regulated by differential stability and expression. Here we describe the identification of the HCF107 gene that is involved in the 5,-end processing/stability and/or translation of the psbH gene and in the translation of the psbB gene. HCF107 is an RNA-TPR-containing protein with 11 RTPRs that are tandemly arranged. A single mutation in the third RTPR that changes a conserved alanine residue to a threonine affects both 5,-end-processed psbH transcript accumulation as well as psbB translation, resulting in disruption of PSII and seedling lethal plants. The protein is localized to the plastid membranes and is present as part of a multi-subunit complex in the range of 60,190 and 600,800 kDa. HCF107 thus represents a new member of the growing helical repeat family of proteins that seem to play a gene-specific role in regulating plastidial gene expression and biogenesis. [source]