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Different Geographical Locations (different + geographical_locations)
Selected AbstractsPolymorphisms in the sequences of Marteilia internal transcribed spacer region of the ribosomal RNA genes (ITS-1) in Spain: genetic types are not related with bivalve hostsJOURNAL OF FISH DISEASES, Issue 6 2005B Novoa Abstract Marteilia refringens is a protozoan parasite causing a disease notifiable to the Office International des Epizooties (OIE) and its distribution has implications for the transfer of live animals. The internal transcribed spacer-1 (ITS-1) from Marteilia clones contains polymorphism. Digestion with HhaI reveals two different restriction profiles, previously referred as ,O' (Marteilia from oyster or Marteilia refringens) and ,M' (Marteilia from mussels or Marteilia maurini). The aim of the present work was to determine whether the two previously described Marteilia molecular types (O and M) exist in the Iberian Peninsula and the strictness of the association with their bivalve host species. The sequence variability in the ITS-1 of Marteilia species was studied in mussels, Mytilus galloprovincialis, and flat oysters, Ostrea edulis, from different geographical locations in Spain, to establish the existence and the distribution of different species or molecular types. Although there were two distinct evolutionary lineages that corresponded more or less strictly with the ,M' and ,O' types, it was evident from the estimated phylogeny that some ,O' types have switched to ,M' type, and vice versa. Moreover, ,O' types were found in mussels and ,M' types were found in oysters, which suggests that there have been several cross-species transmissions of Marteilia between mussels and oysters. [source] Characterization of the Coat Protein Gene of Cymbidium mosaic virus Isolates from IndiaJOURNAL OF PHYTOPATHOLOGY, Issue 5 2006A. R. Sherpa Abstract The variability in the coat protein (CP) gene sequence of Cymbidium mosaic virus (CymMV) that naturally infects orchids worldwide was investigated. Samples were collected from different regions of India, and the gene encoding the CP of nine isolates was specifically amplified by reverse-transcription polymerase chain reaction. The amplified product obtained was cloned, sequenced and multiple sequence alignment of deduced amino acid (aa) sequences revealed considerable homology to CymMV isolates from other countries. The nucleotide sequences and the amino acid sequences were found to be 85,100% identical and 65,100% respectively. Such high sequence conservation suggests that the CymMV CP gene is highly conserved and is a suitable candidate for the development of diagnostic procedures and to provide transgenic resistance to orchids cultivated in different geographical locations. Although recombination is not common among CymMV isolates, one isolate from Cymbidium was found to be a recombinant between a Korean and a Thai isolate of the virus. IHBT communication no: 0451. [source] Cichorium intybus L , cultivation, processing, utility, value addition and biotechnology, with an emphasis on current status and future prospectsJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 5 2001Harsh Pal Bais Abstract Cultivation of chicory plants for various applications, such as utilisation of the root biomass for preparation of a coffee adjuvant, utilisation as a vegetable and, recently, utility of the plants for important phytochemicals, has received global attention. Chicory is widely grown in countries of different geographical locations owing to the economic importance of this crop. This review addresses cultivation, utility, phytochemical studies and pharmacological aspects, with an emphasis on biotechnological developments in recent years and safety evaluation of genetically modified chicory crops. These aspects are dealt with in detail to bring out the current status and future prospects of cultivation and utility of this economically important crop. © 2001 Society of Chemical Industry [source] Systematic review and meta-analysis: enhanced efficacy of proton-pump inhibitor therapy for peptic ulcer bleeding in Asia , a post hoc analysis from the Cochrane CollaborationALIMENTARY PHARMACOLOGY & THERAPEUTICS, Issue 9 2005G. I. Leontiadis Summary Background :,Proton-pump inhibitors reduce re-bleeding rates after ulcer bleeding. However, there is significant heterogeneity among different randomized-controlled trials. Aim :,To see whether proton-pump inhibitors for ulcer bleeding produced different clinical outcomes in different geographical locations. Methods :,This was a post hoc analysis of our Cochrane Collaboration systematic review and meta-analysis of proton-pump inhibitor therapy for ulcer bleeding. Sixteen randomized-controlled trials conducted in Europe and North America were pooled and re-analysed separately from seven conducted in Asia. We calculated pooled rates for 30-day all-cause mortality, re-bleeding and surgical intervention and derived odds ratios and numbers needed to treat with 95% confidence intervals. Results :,There was no significant heterogeneity for any outcome. Reduced all-cause mortality was seen in the Asian randomized-controlled trials (odds ratios = 0.35; 95% confidence interval: 0.16,0.74; number needed to treat = 33), but not in the others (odds ratios =,1.36; 95% confidence interval: 0.94,1.96; number needed to treat , incalculable). There were significant reductions in re-bleeding and surgery in both sets of randomized-controlled trials, but the effects were quantitatively greater in Asia. Conclusions :,Proton-pump inhibitor therapy for ulcer bleeding has been more efficacious in Asia than elsewhere. This may be because of an enhanced pharmacodynamic effect of proton-pump inhibitors in Asian patients. [source] Black currant reversion virus, a mite-transmitted nepovirusMOLECULAR PLANT PATHOLOGY, Issue 3 2004Petri Susi SUMMARY Taxonomy: Black currant reversion virus (BRV) is the first identified mite-transmitted member of the genus Nepovirus (family Comoviridae). A few systematic studies have been performed to compare virus isolates from different geographical locations. Physical properties: Purified preparations contain two closely sedimenting centrifugal components (B and M for RNA1 and RNA2, respectively) at varying ratios, and occasionally a T component (for satellite RNA). The BRV capsids have a diameter of 27 nm and they are putatively composed of 60 copies of a single species of capsid (coat) protein assembled in an icosahedral lattice. Diluted plant sap loses its infectivity within 1 day at 20 °C and in 4,8 days at 4 °C. Hosts: The natural host range of BRV is limited; it infects black currant (Ribes nigrum L.) and some related Ribes species. The transmission of the virus is by the eriophyid gall mite of black currant (Cecidophyopsis ribis). A number of herbaceous plants can be infected experimentally. BRV is the agent of black currant reversion disease (BRD), which is economically the most significant virus disease in Ribes species. BRV and BRD occur widely in locations where black currant is cultivated commercially. [source] Analysis of metabolic variation and galanthamine content in Narcissus bulbs by 1H NMR,PHYTOCHEMICAL ANALYSIS, Issue 1 2010Andrea Lubbe Abstract Introduction , Galanthamine is a benzazepine alkaloid used as a drug to relieve symptoms of Alzheimer's disease. For pharmaceutical use this natural product has been extracted from the plant Leucojum aestivum (Amaryllidaceae) or produced synthetically. Limited supply of the natural source and high cost of synthetic production has led to a search for alternative sources of galanthamine. The bulbs of Narcissus pseudonarcissus (Amaryllidaceae) have been identified as a potential source of raw material for galanthamine extraction. Since inconsistent chemical composition can be an issue with medicinal plant material, it is of interest to know whether large variations occur between Narcissus bulbs grown in different geographical locations. Objective , To evaluate whether large differences exist in the overall metabolic profiles of Narcissus bulbs grown in the two most important cultivation regions. Methodology , 1H NMR and principal component analysis were used for an unbiased comparison of the bulb samples. Results , Overall metabolite profiles were quite similar, but galanthamine levels could slightly discriminate samples by geographical region. 1H NMR was used for quantitation of galanthamine, and was found to be comparable to quantitation by HPLC. Compared with conventional chromatographic methods, sample preparation for 1H NMR analysis is simple and rapid, and only a small amount of plant material is required. Conclusions , Since useful qualitative and quantitative information about the metabolic state of Narcissus bulbs can be obtained by 1H NMR, this method is useful for agricultural applications, and for quality control of raw material used in the pharmaceutical industry. Copyright © 2009 John Wiley & Sons, Ltd. [source] Variation in virus populations and growth characteristics of two sugarcane cultivars naturally infected by Sugarcane yellow leaf virus in different geographical locationsPLANT PATHOLOGY, Issue 5 2007Y. Abu Ahmad Two sugarcane cultivars (R570 and SP71-6163) naturally infected by Sugarcane yellow leaf virus (SCYLV) were each imported from several geographical locations into a sugarcane yellow leaf-free environment (Montpellier, France). Plants were grown as plant cane for 5,6 months and the experiment was repeated for three consecutive years (2003,2005) in a greenhouse. Several sugarcane-growth and disease characteristics were monitored to identify variation in pathogenicity of SCYLV. Depending on their geographical origin, sugarcane cvs R570 and SP71-6163 were infected by SCYLV genotypes BRA-PER or REU, or a mixture of the two. Severity of symptoms did not vary between plants of cv. R570, but variation in disease severity between plants of cv. SP71-6163 from different geographical locations suggested the occurrence of pathogenic variants of SCYLV. For each sugarcane cultivar, differences in stalk length, number of stalk internodes, virus titre in the top visible dewlap leaf, and percentage of infection of leaf and stalk phloem vessels were also found between plants from different geographical origins. However, these differences were not always reproducible from one year to another, suggesting occurrence of different plant responses to SCYLV isolates under varying environmental conditions. [source] Population structure of the Southeast Asian river catfish Mystus nemurusANIMAL GENETICS, Issue 6 2003S. Usmani Summary A total of 143 microsatellites were isolated from Mystus nemurus using a 5, anchored polymerase chain reaction technique or the random amplified hybridization microsatellite method, the first set of microsatellite markers developed for the Southeast Asian river catfish. Twenty polymorphic microsatellite loci were used as markers for population characterization of M. nemurus from six different geographical locations in Malaysia (Perak, Kedah, Johor, UPM, Sarawak and Terengganu). The number of alleles per locus ranged from 2 to 11 with 6.3 as the average number of alleles per locus. Characterization of the populations showed relatively high levels of genetic variation compared with previous studies using allozyme markers. The highest genetic similarity was found between Perak and Kedah, while the highest genetic distance was found between Terengganu and Kedah. The majority of clustering was in accordance with geographical locations and the histories of the populations. Microsatellite analysis indicated that the Sarawak population might be genetically closer to the Peninsular Malaysian populations than has been previously shown by other molecular marker studies. [source] Surveys reveal the occurrence of phytoplasmas in plants at different geographical locations in PeruANNALS OF APPLIED BIOLOGY, Issue 1 2009J. Hodgetts Abstract Two independent surveys were performed in Peru during February and November 2007 to detect the presence of phytoplasmas within any crops showing symptoms resembling those caused by phytoplasmas. Molecular identifications and characterisations were based on phytoplasma 16S and 23S rRNA genes using nested PCR and terminal restriction fragment length polymorphism (T-RFLP). The surveys indicated that phytoplasmas were present in most of the locations sampled in Peru in both cultivated crops, including carrots, maize, native potatoes, improved potato, tomato, oats, papaya and coconut, and in other plants such as dandelion and the ornamental Madagascar periwinkle (Catharanthus roseus). Phylogenetic analysis of the sequences confirmed that while most of the isolates belong to the 16SrI aster yellows group, which is ubiquitous throughout other parts of South America, one isolate from potato belongs to the 16SrII peanut witches' broom group, and one isolate from tomato and one from dandelion belong to the 16SrIII X-disease group. The use of T-RFLP was validated for the evaluation of phytoplasma-affected field samples and provided no evidence for mixed infection of individual plants with more than one phytoplasma isolate. These data represent the first molecular confirmation of the presence of phytoplasmas in a broad range of crops in Peru. [source] Regional differences among employed nurses: A Queensland studyAUSTRALIAN JOURNAL OF RURAL HEALTH, Issue 4 2009Tim Henwood Abstract Objective:,To ascertain differences in the working lives of geographically dispersed nurses. Design:,Cross-sectional. Setting:,Registered, enrolled and assistants-in-nursing members of the Queensland Nurses' Union employed in nursing in Queensland, Australia. Participants:,A total of 3000 members of the Union, equally stratified by sector (public, private, aged care). Among them, 1192 responded and 1039 supplied postcodes matching the Australian Standard Geographical Classification. Main outcome measures:,Statistically significant differences in working lives of nurses employed in different geographical locations. Results:,Nurses in outer regional/remote/very remote localities are more likely to be employed as permanent full-time staff and self-report higher levels of work stress. These levels could be explained by: lack of replacement staff for leave, longer working and on call hours and lack of support for new staff. Distance remains a major barrier to accessing continuing professional education. However, outer regional/remote/very remote nurses were more likely to be provided employer support for professional education. Inner regional nurses were more likely to work part time, would work more hours if offered and were more likely to have taken a break from nursing as a result of family commitments. Conclusion:,The data confirm that current policies are not addressing the differences in the working lives of geographically dispersed nurses. Policies addressing orientation, mentoring and workloads should be implemented to address these issues. [source] Facial skin fluorescence as a marker of the skin's response to chronic environmental insults and its dependence on ageBRITISH JOURNAL OF DERMATOLOGY, Issue 1 2006G.N. Stamatas Summary Background, Throughout life facial skin is exposed to a variety of adverse environmental conditions and is constantly required to repair itself. The rate of epidermal cell proliferation is indicative of the skin's repair rate and can be monitored noninvasively in vivo using skin intrinsic fluorescence markers. Objectives, The goal of the present study was to assess the effects of ageing, geographical region, ethnic origin and season on the ability of facial skin to repair itself in the presence of chronic environmental insults using in vivo fluorescence spectroscopy. Methods, Skin fluorescence emission was measured on the cheeks of 522 individuals in winter and repeated in summer in five different geographical locations in the Asia-Pacific region. Fluorescence emission was also measured from 80 caucasians of fair complexion in the United States (New Jersey area) on the face and on a relatively protected area (upper inner arm). The age range of the participants was 14,75 years. Results, We found that epidermal proliferation rates decrease monotonically with age, while the fluorescence of collagen and elastin cross-links increases with age indicating accumulation of advanced glycation end-products. These trends were independent of geographical region, ethnic origin and season of measurement. Epidermal proliferation rates of facial skin were higher than those of unexposed sites; they may be 10 times higher in younger (second decade) than in older (seventh decade) individuals, and they decrease with age at rates 10 times faster compared with those of unexposed sites. Conclusions, This is the first time that epidermal proliferation and its dependence on ageing have been measured noninvasively on the human face. The higher tryptophan fluorescence values on the face vs. the protected site are indicative of accelerated rates of epidermal proliferation in the presence of chronic environmental insults. The repair potential of facial skin, i.e. its ability to maintain high proliferation rates, is maximal in younger populations and gradually decreases with age. [source] Risk factors for extended-spectrum ,-lactamase positivity in uropathogenic Escherichia coli isolated from community-acquired urinary tract infectionsCLINICAL MICROBIOLOGY AND INFECTION, Issue 2 2010Ö. K. Azap Clin Microbiol Infect 2010; 16: 147,151 Abstract The aim of this prospective cohort study was to determine the risk factors for community-acquired urinary tract infections (UTIs) caused by extended-spectrum ,-lactamase (ESBL)-positive Escherichia coli and the distribution of the ESBL enzyme types. Structured forms were filled in for patients diagnosed with community-acquired UTI in four different geographical locations in Turkey. The forms and the isolates were sent to the central laboratory at Baskent University Hospital, Ankara. Antimicrobial susceptibility was determined according to the CLSI criteria. PCR and DNA sequencing were used to characterize the blaTEM, blaCTX-M and blaSHV genes. Multivariate analysis was performed using logistic regression. A total of 510 patients with UTI caused by Gram-negative bacteria were included in this study. ESBLs were detected in 17 of 269 (6.3%) uropathogenic E. coli isolates from uncomplicated UTIs and 34 of 195 (17.4%) E. coli isolates from complicated UTIs (p <0.001). According to multivariate analysis, more than three urinary tract infection episodes in the preceding year (OR 3.8, 95% CI 1.8,8.1, p <0.001), use of a ,-lactam antibiotic in the preceding 3 months (OR 4.6, 95% CI 2.0,0.7, p <0.001) and prostatic disease (OR 9.6, 95% CI 2.1,44.8, p 0.004) were found to be associated with ESBL positivity. The percentages of isolates with simultaneous resistance to trimethoprim,sulphamethoxazole, ciprofloxacin and gentamicin were found to be 4.6% in the ESBL-negative group and 39.2% in the ESBL-positive group (p <0.001). Forty-six of 51 ESBL-positive isolates (90.2%) were found to harbour CTX-M-15. Therapeutic alternatives for UTI, particularly in outpatients, are limited. Further clinical studies are needed to guide the clinicians in the management of community-acquired UTIs. [source] | ||||||||||||||||