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Different Combinations (different + combination)
Selected AbstractsProspective non-randomized study of preoperative concurrent platinum plus 5-fluorouracil-based chemoradiotherapy with or without paclitaxel in esophageal cancer patients: long-term follow-upDISEASES OF THE ESOPHAGUS, Issue 2 2010M. Zemanova SUMMARY Combined modality treatment for esophageal carcinoma seems to improve survival over surgery alone. Different combinations of cytotoxic drugs have been studied to improve antitumor efficacy and limit the toxicity of chemoradiotherapy (CRT) with inconsistent results. We present a prospective study of neoadjuvant CRT with or without paclitaxel in chemotherapy schedule. One hundred seven patients (93 males, 14 females), median age 59 years (range 44,76), with operable esophageal cancer were enrolled. They received the following neoadjuvant therapy: Carboplatin, area under curve (AUC) = 6, intravenously on days 1 and 22, 5-fluorouracil (5-FU), 200 mg/m2/day, continuous infusion on days 1 to 42, radiation therapy 45 grays/25fractions/5 weeks beginning on day 1. Forty-four patients (41%) were furthermore non-randomly assigned to paclitaxel 200 mg/m2/3 h intravenously on days 1 and 22. Nutritional support from the beginning of the treatment was offered to all patients. Surgery was done within 4,8 weeks after completion of CRT, if feasible. All patients were evaluated for grade 3 plus 4 toxicities: leukopenia (28%), neutropenia (30%), anemia (6%), thrombocytopenia (31%), febrile neutropenia (6%), esophagitis (24%), nausea and vomiting (7%), pneumotoxicity (8%). Seventy-eight patients (73%) had surgery and 63 of them were completely resected. Twenty-two patients (20%) achieved pathological complete remission, and additional 20 (19%) had node-negative and esophageal wall-positive residual disease. There were 10 surgery-related deaths, mostly due to pulmonary insufficiency. Twenty-nine patients were not resected, 15 for early progression, 14 for medical reasons or patient refusal. After a median follow-up of 52 months (range 27,80), median survival of 18.0 months and 1-, 2-, 3- and 5-year survival of 56.7, 37.5, 27.0 and 21% was observed in the whole group of 107 patients. Addition of paclitaxel to carboplatin and continual infusion of FU significantly increased hematologic and non-hematologic toxicity, but treatment results as overall survival or time to progression did not differ significantly in groups with and without paclitaxel. Patients achieving pathological complete remission or nodes negativity after neoadjuvant therapy had favorable survival prognosis, whereas long-term prognosis of node positive patients was poor. Distant metastases prevailed as a cause of the treatment failure. Factors significant for survival prognosis in multivariate analysis were postoperative node negativity, performance status, and grade of dysphagia. Addition of paclitaxel to carboplatin and continual FU significantly increased hematologic and non-hematologic toxicity without influencing efficacy of the treatment. This study confirmed improved prognosis of patients after achieving negativity of nodes. Distant metastases prevailed as cause of the treatment failure. Prospectively, it is important to look for a therapeutic combination with better systemic effect. [source] Electrospun Silk Fibroin Mats for Tissue EngineeringENGINEERING IN LIFE SCIENCES (ELECTRONIC), Issue 3 2008A. Alessandrino Abstract Processing Silk Fibroin (SF) with electrospinning (ES) offers a very attractive opportunity for producing a variety of 2D and 3D matrices with great potential for tissue regeneration and repair due to the superior biocompatibility and mechanical properties of SF. Different combinations of ES parameters were explored to investigate the best experimental set-up related to the dimension and uniformity of the fibers in the electrospun silk fibroin (ES-SF) mats. Using SEM it was found that the ES-SF mats contain uniform fibers with a diameter in the nanometric range obtained by electrospinning a 7.5,% w/v SF solution in formic acid, with an electric field of 2.4,kV/cm and a spinneret-collector distance of 10,cm. FT-IR and DSC analyses were performed to investigate the structure of the ES-SF mats before and after immersion in methanol for different times (5, 10, and 15,min). The methanol treatment was able to promote the crystallization of SF by conformational transition of random coil and other poorly ordered conformations (turns and bends) to the ,-sheet structure. The degree of crystallinity was enhanced as shown by the trend of both the FT-IR crystallinity index and the melting/decomposition peak temperature (from DSC). To study the cytocompatibility of ES-SF mats, tests with L929 murine fibroblasts were carried out. Samples were seeded with the cells and incubated for 1, 3, and 7,days at 37,°C. At each time point, SEM investigations and Alamar blue tests were performed. The SEM images showed cell adhesion and proliferation just after 1,day and cell confluence at 7,days. Alamar blue test demonstrated that there were very low differences between cell viability on ES-SF mats and the tissue culture plastic control. [source] Hyporheic Exchange in Mountain Rivers II: Effects of Channel Morphology on Mechanics, Scales, and Rates of ExchangeGEOGRAPHY COMPASS (ELECTRONIC), Issue 3 2009John M. Buffington We propose that the mechanisms driving hyporheic exchange vary systematically with different channel morphologies and associated fluvial processes that occur in mountain basins, providing a framework for examining physical controls on hyporheic environments and their spatial variation across the landscape. Furthermore, the spatial distribution of hyporheic environments within mountain catchments represents a nested hierarchy of process controls. Large-scale process drivers (geology, climate, fire, and land use) impose a suite of watershed conditions (topography, streamflow, sediment supply, and vegetation) on the fluvial system. Different combinations of imposed watershed conditions result in different reach-scale channel morphologies (e.g. step-pool, pool-riffle, and braided) that, in turn, structure hyporheic processes (e.g. pressure divergence, spatial variation of hydraulic conductivity) and resultant hyporheic environments (scales and rates of hyporheic exchange). Consequently, a holistic view of natural and anthropogenic drivers over a range of spatial and temporal scales is needed for understanding hyporheic ecosystems. [source] Different combinations of salts affect the growth and bacteriocin production by Lactobacillus salivarius CRL 1328JOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY, Issue 1 2010María Silvina Juárez Tomás Abstract BACKGROUND: The culture medium for optimal growth of vaginal Lactobacillus salivarius CRL 1328 is different from that for optimal bacteriocin production. To simultaneously obtain high amount of biomass and bacteriocin of this microorganism, the effects of different basal culture media and salts on both responses were evaluated. The study was performed by using a complete factorial experimental design 26, with central points. Sixty-four different growth media, which resulted from the combinations of two basal culture media and two concentrations of five salts (ammonium citrate, sodium acetate, MgSO4, MnSO4, and K2HPO4) were assayed. RESULTS: Only the addition of MnSO4 to each culture medium significantly stimulated the growth of L. salivarius. The presence of sodium acetate or MgSO4 stimulated the bacteriocin production, while MnSO4 and K2HPO4 exerted an inhibitory effect. However, the simultaneous addition of MnSO4 and sodium acetate to both basal culture media allowed high bacteriocin levels to be reached, attenuating the inhibitory effect of Mn2+. CONCLUSIONS: The application of a complete experimental design contributed to simultaneous optimization of the biomass and bacteriocin production of L. salivarius CRL 1328. The results obtained are potentially applicable to the technological production of probiotic bacteria and antagonistic substance to be included in a probiotic pharmaceutical product. Copyright © 2009 Society of Chemical Industry [source] EFFECT OF SURFACTANT GEL AND GUM COMBINATIONS ON DOUGH RHEOLOGICAL CHARACTERISTICS AND QUALITY OF BREADJOURNAL OF FOOD QUALITY, Issue 5 2004M.H. AZIZI ABSTRACT Sodium stearoyl-2-lactylate (SSL), diacetyl tartaric acid esters of monoglyceride (DATEM), glycerol monostearate (GMS) and distilled glycerol monostearate (DGMS) surfactant gels were made with water. Addition of surfactant gels decreased water absorption by the bread while xanthan, karaya, guar and locust bean gums increased the same. Only DGMS or GMS and gum combinations further improved water absorption. All the gums except for guar along with surfactant gels improved dough stability. Both surfactant gels and gums improved the extensograph dough properties of wheat flour to varying degrees. Alveograph characteristics of wheat flour improved to varying extents with surfactant gels while the gums influenced the viscoelastic properties in differing ways. Different combinations of surfactant gels and gums showed varied influences on rapid visco analyzer characteristics of wheat flour. Both surfactant gels and gums improved the bread making quality. Among surfactants, SSL in combination with gums, and among gums locust bean in combination with surfactant gels improved the bread making quality of wheat flour to a maximum extent. [source] Maximum entropy method and charge flipping, a powerful combination to visualize the true nature of structural disorder from in situ X-ray powder diffraction dataACTA CRYSTALLOGRAPHICA SECTION B, Issue 2 2010Ali Samy In a systematic approach, the ability of the Maximum Entropy Method (MEM) to reconstruct the most probable electron density of highly disordered crystal structures from X-ray powder diffraction data was evaluated. As a case study, the ambient temperature crystal structures of disordered ,-Rb2[C2O4] and ,-Rb2[CO3] and ordered ,-K2[C2O4] were investigated in detail with the aim of revealing the `true' nature of the apparent disorder. Different combinations of F (based on phased structure factors) and G constraints (based on structure-factor amplitudes) from different sources were applied in MEM calculations. In particular, a new combination of the MEM with the recently developed charge-flipping algorithm with histogram matching for powder diffraction data (pCF) was successfully introduced to avoid the inevitable bias of the phases of the structure-factor amplitudes by the Rietveld model. Completely ab initio electron-density distributions have been obtained with the MEM applied to a combination of structure-factor amplitudes from Le Bail fits with phases derived from pCF. All features of the crystal structures, in particular the disorder of the oxalate and carbonate anions, and the displacements of the cations, are clearly obtained. This approach bears the potential of a fast method of electron-density determination, even for highly disordered materials. All the MEM maps obtained in this work were compared with the MEM map derived from the best Rietveld refined model. In general, the phased observed structure factors obtained from Rietveld refinement (applying F and G constraints) were found to give the closest description of the experimental data and thus lead to the most accurate image of the actual disorder. [source] Effect of feeding scheme and prey density on survival and development of Chinese mitten crab Eriocheir sinensis zoea larvaeAQUACULTURE RESEARCH, Issue 6 2008Li-ying Sui Abstract The effect of feeding scheme and prey density on survival and development of Eriocheir sinensis zoea larvae was studied in three experiments. Different combinations and densities of rotifers (Brachionus rotundiformis) and newly hatched Artemia nauplii were fed to zoea larvae. Average survival at each stage, larval development (larval stage index, LSI), duration of zoeal stage and individual megalopa dry weight were compared among treatments. This study revealed that, under the experimental conditions, rotifers should be replaced with Artemia between the zoea 3 (Z3) and the zoea 4 (Z4) stage. The optimal rotifer feeding densities for zoea 1 (Z1) and zoea 2 (Z2) were 15 and 20 mL,1 respectively, while the optimal Artemia feeding density for Z3, Z4 and zoea 5 (Z5) was 3, 5 and 8 mL,1 respectively. Further trials in production scale are recommended. [source] A simulated auto-thermal membrane reformer process for a PEM fuel cell micro cogeneration unitASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING, Issue 3 2009Dr. Atilla Ersöz Abstract There are several methods of producing hydrogen-rich gas from fossil resources such as natural gas or naphtha, for example, steam reforming, partial oxidation and auto-thermal reforming. In this paper, an integrated ATR membrane reactor system was simulated. The effect of operating parameters on the product distribution, fuel cell hydrogen utilization and the net electric efficiency of the overall system were discussed. The overall system was integrated with a 1-kWe PEM fuel cell. The ASPEN-HYSIS 3.2 software has been utilized for the simulations and calculations of the fuel processing reactions. Natural gas fuel has been used as feedstock and applied to the simulated flow-sheet model. It was desired to produce hydrogen-rich gas with a low CO formation using an autothermal membrane reformer. A very low CO content with higher content of hydrogen was provided by the membrane reformer, eliminating the use of the conventional preferential oxidation (PrOx) reactor. Different combinations of TATR, S/C, O2/C ratios and UH2 have been parametrically studied. Fuel processing efficiency and net electrical efficiency of all selected operating conditions have been calculated as well. Results indicate that the system parameters are very critical for the appropriate operation of the residential cogeneration system with ATR membrane unit. Copyright © 2009 Curtin University of Technology and John Wiley & Sons, Ltd. [source] Direct enantiomeric resolution of (±)-atenolol, (±)-metoprolol, and (±)-propranolol by impregnated TLC using L -aspartic acid as chiral selectorBIOMEDICAL CHROMATOGRAPHY, Issue 4 2003R. Bhushan Abstract Resolution of three commonly used ,-blockers, (±)-atenolol, (±)-metoprolol and (±)-propranolol, into their enantiomers has been achieved using normal-phase TLC on silica gel plates impregnated with L -aspartic acid as the chiral selector. Different combinations of acetonitrile,methanol,water as mobile phase were found to be successful in resolving the enantiomers. The spots were detected with iodine and the detection limits were found to be 0.26,µg for atenolol and 0.23,µg for each of metoprolol and propranolol as racemate. Copyright © 2003 John Wiley & Sons, Ltd. [source] An educational computer tool for simulating long-term soil erosion on agricultural landscapesCOMPUTER APPLICATIONS IN ENGINEERING EDUCATION, Issue 3 2009F. J. Jiménez-Hornero Abstract Due to its economic and environmental impacts, soil erosion has been a major concern to farmers, engineers and policy makers in recent years. Water and tilling are two of the main agents responsible for this phenomenon and considerable efforts have been made to model them in previous work but not with educational purposes. A computer tool for facilitating any user's simulation of long-term landscape evolution in a plot due to the combined action of water and tillage erosion is presented here. It integrates a graphic user interface with two well-verified erosion models, each one independently devoted to reproduce the effects of water and tilling. This computer tool permits to the student the consideration of the erosivity index and the presence of a crop in the plot, when simulating water erosion, as well as the planning of a different type of tilling each year. Each kind of tilling corresponds to a different combination of tillage tools with their own date, tillage depth and tillage direction. A handy ASCII (XYZ) file is generated containing the long-term soil erosion spatial pattern as result. From this information, the student can derive other results that will help to understand soil erosion. An example is presented here with the aim of showing how to use this computer tool to simulate this phenomenon on an agricultural landscape with a complex topography. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 17: 253,262, 2009; Published online in Wiley InterScience (www.interscience.wiley.com); DOI 10.1002/cae.20193 [source] Burning and cutting: Identifying the traits of individuals with an enduring propensity to tan and to undergo cosmetic surgeryJOURNAL OF CONSUMER BEHAVIOUR, Issue 5 2009John C. Mowen The research investigated the trait predictors of the propensity to use two beautification procedures that have the potential to harm health , the propensities to tan and to obtain cosmetic surgery. The results of a survey of 231 adult consumers revealed that a different combination of hierarchically arranged traits was predictive of the two procedures. These results suggest that different strategies are required in order to market or demarket tanning and plastic surgery. The research also made methodological contributions by revealing that two forms of vanity identified in the literature , physical view vanity and physical concern vanity , represent two different constructs rather than two dimensions of an underlying construct. Copyright © 2009 John Wiley & Sons, Ltd. [source] A cross-national examination of consumer perceived risk in the context of non-deceptive counterfeit brandsJOURNAL OF CONSUMER BEHAVIOUR, Issue 1 2008Dr. Cleopatra Veloutsou This study investigates the relationships between six identified dimensions of perceived risk and the overall risk, and also their explanatory power to the overall risk when purchasing counterfeit brands in two different contexts. Using a self-administered instrument, a total of 525 responses were collected, 230 from the UK and 295 from China. The results support the hypothesised significant interrelationship between the dimensions of perceived risk in both contexts. However there are some differences. The models have a different exploratory power, with the one based on the British sample being stronger, while a somewhat different combination of the risk dimensions seems to predict the overall risk in each context. The psychological risk is the only dimension of risk that with no doubt contributes to the formation of the overall risk in both contexts. Although one could argue that when purchasing counterfeits social risk could be an issue, the evidence reported in this study does not support the view. Copyright © 2008 John Wiley & Sons, Ltd. [source] Inflammatory bowel disease: Established and evolving considerations on its etiopathogenesis and therapyJOURNAL OF DIGESTIVE DISEASES, Issue 5 2010Anja SCHIRBEL Modern studies of inflammatory bowel disease (IBD) pathogenesis have been pursued for about four decades, a period of time where the pace of progress has been steadily increasing. This progress has occurred in parallel with and is largely due to developments in multiple basic scientific disciplines that range from population and social studies, genetics, microbiology, immunology, biochemistry, cellular and molecular biology, and DNA engineering. From this cumulative and constantly expanding knowledge base the fundamental pillars of IBD pathogenesis appear to have been identified and consolidated during the last couple of decades. Presently there is a general consensus among basic IBD investigators that both Crohn's disease (CD) and ulcerative colitis (UC) are the result of the combined effects of four basic components: global changes in the environment, the input of multiple genetic variations, alterations in the intestinal microbiota, and aberrations of innate and adaptive immune responses. There is also agreement on the conclusion that none of these four components can by itself trigger or maintain intestinal inflammation. A combination of various factors, and most likely of all four factors, is probably needed to bring about CD or UC in individual patients, but each patient or set of patients seems to have a different combination of alterations leading to the disease. This would imply that different causes and diverse mechanisms underlie IBD, and this could also explain why every patient displays his or her own clinical manifestations and a personalized response to therapy, and requires tailored approaches with different medications. While we are becoming increasingly aware of the importance of this individual variability, we have only a superficial notion of the reasons why this occurs, as hinted by the uniqueness of the genetic background and of the gut flora in each person. So, we are apparently facing the paradox of having to deal with the tremendous complexity of the mechanisms responsible for chronic intestinal inflammation in the setting of each patient's individuality in the response to this biological complexity. This obviously poses considerable challenges to reaching a full understanding of IBD pathogenesis, but being aware of the difficulties is the first step in finding answers to them. [source] Genetic heterogeneity in rheumatoid arthritis mouse models induced by extrinsic and intrinsic factorsPATHOLOGY INTERNATIONAL, Issue 6 2010Shinichi Mizuki A cumulative effect of the susceptibility genes with polymorphic alleles may be responsible for rheumatoid arthritis (RA). The objective of this study was to clarify whether susceptibility to RA is under the control of common allelic loci between two different RA models induced by extrinsic and intrinsic factors, collagen-induced arthritis (CIA) in DBA/1 mice and arthritis in MRL/Mp (MRL) mice associated with the Fas deficient mutant gene, Faslpr, respectively. CIA was examined in mice of parental DBA/1 and MRL, (MRL × DBA/1) F1 and (MRL × DBA/1) F2 progenies. In genome-wide screening of the severity in the F2 using microsatellite markers, significant linkage was observed on chromosomes 5 and 17 at map position of D5Mit259 and H-2, respectively, associated with DBA/1 alleles, while there was no loci associated with arthritis of MRL- Faslpr mice previously identified. In a quantitative trait locus (QTL) analysis, the locus on chromosome 5 showed the highest peak at map position 35 cM (LOD score 6.0). This study may indicate that the arthritis induced by extrinsic and intrinsic factors is under the control of a different combination of susceptibility genes with common and different alleles, possibly simulating the genetic heterogeneity of RA. [source] Comparative and evolutionary dimensions of the energetics of human pregnancy and lactationAMERICAN JOURNAL OF HUMAN BIOLOGY, Issue 5 2002D.L. Dufour The purpose of this article is to compare the energetics of reproduction for human and other primates in order to evaluate the extent to which human reproductive energetics are distinct from other primates and other large-bodied placental mammals. The article also evaluates the energetics of human and primate gestation and lactation using data from a variety of different populations living under different environmental circumstances. Energetics refers to energy intake and expenditure, and changes in body fat stores. Human and nonhuman primates have longer periods of gestation and lactation and slower prenatal and postnatal growth than other mammals of similar size. This reduces daily maternal energy costs. The development of sizable fat stores is not unique to humans, but fat stores are typically greater in human females and may play a greater role in reproduction. The strategies used to meet the energy costs of pregnancy vary among populations of humans and nonhuman primates and among humans interindividual variability is high. In pregnancy, some increase energy intake but others apparently do not. Increases in metabolic efficiency are evident in some human populations, whereas decreases in physical activity occur, but are not seen in all human or primate populations. Lactation is more energetically costly on a daily basis among humans and nonhuman primates, but has not been as well studied. It appears that both nonhuman and human primates tend to increase energy intake to meet in part the cost of lactation. They also use other strategies such as relying on body tissue stores, reductions in physical activity, and/or increases in metabolic efficiency to meet the remainder of the cost. It is also clear that human females in different populations and different women in the same population use a different combination of strategies to meet the cost of lactation. Am. J. Hum. Biol. 14:584,602, 2002. © 2002 Wiley-Liss, Inc. [source] Thermodynamic Analysis of Receptors Based on Guanidinium/Boronic Acid Groups for the Complexation of Carboxylates, ,-Hydroxycarboxylates, and Diols: Driving Force for Binding and CooperativityCHEMISTRY - A EUROPEAN JOURNAL, Issue 15 2004Sheryl L. Wiskur Dr. Abstract The thermodynamics of guanidinium and boronic acid interactions with carboxylates, ,-hydroxycarboxylates, and diols were studied by determination of the binding constants of a variety of different guests to four different hosts (7,10). Each host contains a different combination of guanidinium groups and boronic acids. The guests included molecules with carboxylate and/or diol moieties, such as citrate, tartrate, and fructose, among others. The Gibbs free energies of binding were determined by UV/Vis absorption spectroscopy, by use of indicator displacement assays. The receptor based on three guanidinium groups (7) was selective for the tricarboxylate guest. The receptors that incorporated boronic acids (8,10) had higher affinities for guests that included ,-hydroxycarboxylate and catechol moieties over guests containing only carboxylates or alkanediols. Isothermal titration calorimetry revealed the enthalpic and entropic contributions to the Gibbs free energies of binding. The binding of citrate and tartrate was investigated with hosts 7,10, for which all the binding events were exothermic, with positive entropy. Because of the selectivity of hosts 8,10, a simple boronic acid (14) was also investigated and determined to be selective for ,-hydroxycarboxylates and catechols over amino acids and alkanediols. Further, the cooperativity of 8 and 9 in binding tartrate was also investigated, revealing little or no cooperativity with 8, but negative cooperativity with 9. A linear entropy/enthalpy compensation relationship for all the hosts 7,10, 14, and the carboxylate-/diol-containing guests was also obtained. This relationship indicates that increasing enthalpy of binding is offset by similar losses in entropy for molecular recognition involving guanidinium and boronic acid groups. [source] Near-infrared dyes for six-color immunophenotyping by laser scanning cytometryCYTOMETRY, Issue 3 2002Andreas O.H. Gerstner Abstract Background To adequately analyze the complexity of the immune system and reduce the required sample volume for immunophenotyping in general, more measurable colors for the discrimination of leukocyte subsets are necessary. Immunophenotyping by the laser scanning cytometer (LSC), a slide-based cytometric technology, combines cell detection based on multiple colors with their subsequent visualization without the need for physical cell sorting. In the present study, the filter setting of the LSC was adapted for the measurement of the far-red emitting dye cyanine 7 (Cy7), thereby increasing the number of measurable commercially available fluorochromes. Methods The optical filters of the LSC were replaced,photomultiplier (PMT) 3/allophycocyanin (APC): 740-nm dichroic long pass, and 670-/55-nm bandpass; PMT 4/Cy7: 810-/90-nm bandpass. Peripheral blood leukocytes were stained directly by fluorochrome-labeled antibodies or by indirect staining. The tandem dyes of Cy7 (phycoerythrin [PE]-Cy7, APC-Cy7) and the fluorochromes fluorescein isothiocyanate (FITC), PE, PE-Cy5, and APC were tested alone and in different combinations. Results With the new filter combination and tandem fluorochromes, Cy7 was measurable at 488-nm (argon laser) or 633-nm (helium-neon laser) excitation. Resolution was in the range of FITC for PE-Cy7 but approximately 30% lower for APC-Cy7; spillover into the respective donor fluorochrome channel for both tandem dyes was prominent. A six-color panel for leukocyte subtyping was designed. Conclusions With this adaptation, it is possible to measure the tandem conjugates PE-Cy7 and APC-Cy7. This new setup opens the way for six-color immunophenotyping by LSC. Cytometry 48:115,123, 2002. © 2002 Wiley-Liss, Inc. [source] ,Salience syndrome' replaces ,schizophrenia' in DSM-V and ICD-11: psychiatry's evidence-based entry into the 21st century?ACTA PSYCHIATRICA SCANDINAVICA, Issue 5 2009J. Van Os Objective:, Japan was the first country to abandon the 19th century term of ,mind-splitting disease' (schizophrenia). Revisions of DSM and ICD are forthcoming. Should the rest of the world follow Japan's example? Method:, A comprehensive literature search was carried out in order to review the scientific evidence for the validity, usefulness and acceptability of current concepts of psychotic disorder. Results:, The discussion about re-classifying and renaming schizophrenia and other psychotic disorders is clouded by conceptual confusion. First, it is often misunderstood as a misguided attempt to change societal stigma instead of an attempt to change iatrogenic stigma occasioned by the use of misleading and mystifying terminology. Second, the debate is misunderstood as purely semantic, whereas in actual fact it is about the core concepts underlying psychiatric nosology. Third, it has been suggested that the debate is political. However, solid scientific evidence pointing to the absence of nosological validity of diagnostic categories lies at the heart of the argument. Fourth, there is confusion about what constitutes a syndrome (a group of symptom dimensions that cluster in different combinations in different people and for which one or more underlying diseases may or may not be found) and a disease (a nosologically valid entity with specific causes, symptoms, treatment and course). Conclusion:, Scientific evidence favours a syndromal system of classification combining categorical and dimensional representations of psychosis. The concept of ,salience' has the potential to make the public recognize psychosis as relating to an aspect of human mentation and experience that is universal. It is proposed to introduce, analogous to the functional-descriptive term ,Metabolic syndrome', the diagnosis of ,Salience syndrome' to replace all current diagnostic categories of psychotic disorders. Within Salience syndrome, three subcategories may be identified, based on scientific evidence of relatively valid and specific contrasts, named Salience syndrome with affective expression, Salience syndrome with developmental expression and Salience syndrome not otherwise specified. [source] Design of multiple tuned mass dampers by using a numerical optimizerEARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 2 2005Nam Hoang Abstract A new method to design multiple tuned mass dampers (multiple TMDs) for minimizing excessive vibration of structures has been developed using a numerical optimizer. It is a very powerful method by which a large number of design variables can be effectively handled without imposing any restriction before the analysis. Its framework is highly flexible and can be easily extended to general structures with different combinations of loading conditions and target controlled quantities. The method has been used to design multiple TMDs for SDOF structures subjected to wide-band excitation. Some novel results have been obtained. To reduce displacement response of the structure, the optimally designed multiple TMDs have distributed natural frequencies and distinct damping ratios at low damping level. The obtained optimal configuration of TMDs was different from the earlier analytical solutions and was proved to be the most effective. A robustness design of multiple TMDs has also been presented. Robustness is defined as the ability of TMDs to function properly despite the presence of uncertainties in the parameters of the system. Numerical examples of minimizing acceleration structural response have been given where the system parameters are uncertain and are modeled as independent normal variates. It was found that, in case of uncertainties in the structural properties, increasing the TMD damping ratios along with expanding the TMD frequency range make the system more robust. Meanwhile, if TMD parameters themselves are uncertain, it is necessary to design TMDs for higher damping ratios and a narrower frequency range. Copyright © 2004 John Wiley & Sons, Ltd. [source] Experimental and theoretical simulations of seismic poundings between two adjacent structuresEARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 4 2003K. T. Chau Abstract Shaking table tests have been carried out to investigate the pounding phenomenon between two steel towers of different natural frequencies and damping ratios, subject to different combinations of stand-off distance and seismic excitations. Both harmonic waves and ground motions of the 1940 El Centro earthquake are used as input. Subjected to sinusoidal excitations, poundings between the two towers could appear as either periodic or chaotic. For periodic poundings, impact normally occurs once within each excitation cycle or within every other excitation cycle. A type of periodic group poundings was also observed for the first time (i.e. a group of non-periodic poundings repeating themselves periodically). Chaotic motions develop when the difference of the natural frequency of the two towers become larger. Under sinusoidal excitations, the maximum relative impact velocity always develops at an excitation frequency between the natural frequencies of the two towers. Both analytical and numerical predictions of the relative impact velocity, the maximum stand-off distance, and the excitation frequency range for pounding occurrences were made and found to be comparable with the experimental observations in most of the cases. The stand-off distance attains a maximum when the excitation frequency is close to that of the more flexible tower. Pounding appears to amplify the response of the stiffer structure but suppress that of the more flexible structure; and this agrees qualitatively with previous shaking table tests and theoretical studies. Copyright © 2003 John Wiley & Sons, Ltd. [source] Behavior of interacting species in vacancy affinity capillary electrophoresis described by mass balance equationELECTROPHORESIS, Issue 16 2008Ying Sun Abstract Vacancy ACE (VACE) is one of the ACE methods, and has been used to study binding interactions between different biomolecules. Thermodynamic binding constants can be estimated with nonlinear regression methods. With a highly efficient computer simulation program (SimDCCE), it is possible to demonstrate the detailed behaviors of each species during the interaction process under different conditions. In this work, thirteen scenarios in four different combinations of migration orders of the free protein, free drug, and complex formed are studied. The detailed interaction process between protein and ligand is discussed and illustrated based on the mass balance equation, also called mass transfer equation. By properly setting the parameters in the simulation model, the influence of different factors during the interaction process can be well understood. [source] Chronic copper toxicity in the estuarine copepod Acartia tonsa at different salinitiesENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 10 2010Mariana M. Lauer Abstract Chronic Cu toxicity was evaluated in the euryhaline copepod Acartia tonsa. Male and female copepods were exposed (6 d) separately to different combinations of Cu concentration and water salinity (5, 15, and 30 ppt) using different routes of exposure (waterborne, waterborne plus dietborne, and dietborne). After exposure, groups of one male and three female copepods were allowed to reproduce for 24,h. In control copepods, egg production augmented with increasing water salinity. However, egg hatching rate did not change. Copper exposure reduced egg production and hatching rate in all water salinities tested, but the reproductive response was dependent on the route of Cu exposure. Median effective concentration (EC50) values for egg production after waterborne exposure were 9.9, 36.8, and 48.8,µg/L dissolved Cu at water salinities of 5, 15, and 30 ppt, respectively. For waterborne plus dietborne exposure, they were significantly higher (40.1, 63.7, and 109.9,µg /L, respectively). After dietborne exposure, approximately 40% decrease in egg production was observed, independently of Cu concentration and water salinity tested. At water salinities of 5 and 30 ppt, egg hatching rate reduced after waterborne exposure, together or not with the dietborne exposure. At water salinity of 15 ppt, Cu toxicity was only observed after dietborne exposure. Data indicate that egg production is a more reliable reproductive endpoint to measure chronic Cu toxicity in copepods than egg hatching rate in a wide range of water salinities. They also suggest that both water salinity and route of Cu exposure should be taken into account in the development of a chronic biotic ligand model version for estuarine and marine environments. Environ. Toxicol. Chem. 2010;29:2297,2303. © 2010 SETAC [source] Evidence of oxidative stress in bluegill sunfish (Lepomis macrochirus) liver microsomes simultaneously exposed to solar ultraviolet radiation and anthraceneENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 7 2000Jonghoon Choi Abstract Many polycyclic aromatic hydrocarbons (PAHs) are acutely toxic to fish and other aquatic organisms in the presence of environmentally realistic intensities of solar ultraviolet radiation (SUVR). However, the biochemical mechanism of this toxicity is not well established. In this study, increased levels of both reactive oxygen species production and lipid peroxidation were hypothesized as a toxic mechanism. To test this hypothesis, the production of superoxide anion and of a lipid peroxidation product (malondialdehyde) was measured in bluegill sunfish (Lepomis machrochirus) liver microsomes. These microsomes were exposed to a representative phototoxic PAH (anthracene [ANT]) and to SUVR and normal laboratory fluorescent light (FLU) in four different combinations: FLU + no ANT, FLU + ANT, SUVR + no ANT, and SUVR + ANT. The highest mean levels of both superoxide anion and malondialdehyde production were observed in the SUVR + ANT group, and these levels were significantly different (p < 0.05) from those in all other treatment groups. We conclude that the photoinduced toxicity of ANT, and possibly of other phototoxic PAHs, manifests at least in part through lipid peroxidation after increased production of reactive oxygen species. [source] Influence of light energy and power density on the microhardness of two nanohybrid compositesEUROPEAN JOURNAL OF ORAL SCIENCES, Issue 1 2008Kerstin Gritsch The purpose of this study was to investigate the role of light parameters on nanohybrid composite curing. Two nanohybrid resins were cured by two light-emitting diode (LED) devices and by one quartz-tungsten-halogen (QTH) device using different combinations of energy density and power density (8 J cm,2 and 400 mW cm,2; 8 J cm,2 and 1,000 mW cm,2; 16 J cm,2 and 400 mW cm,2; and 16 J cm,2,1,000 mW cm,2). The effects of these combinations on polymerization were assessed by measuring the Vickers microhardness. Data differed for the two composites and varied according to the light parameters and the nature of the curing device. For both resins, an energy density of 16 J cm,2 yielded the best microhardness values at both the top and the bottom of the sample, independently of the power density. When using a lower energy density of 8 J cm,2, a modulated power density was required to achieve proper curing at the bottom of the sample: 8 J cm,2 and 400 mW cm,2 induced greater values at the bottom surface. At an energy density of 16 J cm,2, the power density was not relevant (no significant differences were found between 400 and 1,000 mW cm,2), except when the emission spectra of the light-curing units (LCUs) did not match exactly with the absorption spectra of the photoinitators included in the resins (greatest values with 16 J cm,2 and1,000 mW cm,2). These results suggest that above a certain energy density threshold, the power density may not significantly influence the polymerization kinetics. [source] Personality variable differences between disease clustersEUROPEAN JOURNAL OF PERSONALITY, Issue 2 2003G. Matthews Previous studies of personality and health have focused mainly on the influence of psychological factors on single diseases such as cancer and coronary heart disease (CHD), thereby neglecting the problem of comorbidity (i.e. the combination of different diseases). The main focus of the present study was the discrimination between single- and multiple-disease conditions on the basis of personality traits. An extensive battery of personality scales implicated in health was administered to a sample of n=5133 individuals of both genders between the ages of 40 and 65. Subjects also reported their health or illness status. A factor analysis of the personality scales yielded five dimensions clearly interpretable as "Emotional Lability", "Type A Behaviour", "Behavioural Control", "Locus of Control over Diseases", and "Psychoticism". Hierarchical cluster analyses of the subsample of participants who reported suffering from more than one disease led to eight clusters representing individuals with different combinations of diseases. Generally, there were very few significant differences between healthy and single-disease participants with regard to personality. However, mean factor scores calculated for "Emotional Lability" were higher across the multiple-disease groups than in the healthy and single-disease groups. No other personality factor showed this trend. In general the results reported here show the important role negative affectivity (e.g. Emotional Lability, Neuroticism, Depression) plays in differentiating between single and multiple diseases. Copyright © 2003 John Wiley & Sons, Ltd. [source] Plasticity of hair follicle dermal cells in wound healing and inductionEXPERIMENTAL DERMATOLOGY, Issue 2 2003A. Gharzi Abstract: The capacity of adult hair follicle dermal cells to participate in new follicle induction and regeneration, and to elicit responses from diverse epithelial partners, demonstrates a level of developmental promiscuity and influence far exceeding that of interfollicular fibroblasts. We have recently suggested that adult follicle dermal cells have extensive stem or progenitor cell activities, including an important role in skin dermal wound healing. Given that up to now tissue engineered skin equivalents have several deficiencies, including the absence of hair follicles, we investigated the capacity of follicle dermal cells to be incorporated into skin wounds; to form hair follicles in wound environments; and to create a hair follicle-derived skin equivalent. In our study, we implanted rat follicle dermal cells labelled with a vital dye into ear and body skin wounds. We found that they were incorporated into the new dermis in a manner similar to skin fibroblasts, but that lower follicle dermal sheath also assimilated into hair follicles. Using different combinations of follicle dermal cells and outer root sheath epithelial cells in punch biopsy wounds, we showed that new hair follicles were formed only with the inclusion of intact dermal papillae. Finally by combining follicle dermal sheath and outer root sheath cells in organotypic chambers, we created a skin equivalent with characteristic dermal and epidermal architecture and a normal basement membrane , the first skin to be produced entirely from hair follicle cells. These data support the hypothesis that follicle dermal cells may be important in wound healing and demonstrate their potential usefulness in human skin equivalents and skin substitutes. While we have made progress towards producing skin equivalents that contain follicles, we suggest that the failure of cultured dermal papilla cells to induce follicle formation in wounds illustrates the complex role the follicle dermis may play in skin. We believe that it demonstrates a genuine dichotomy of activity for follicle cells within skin. [source] Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcaniiFEBS JOURNAL, Issue 7 2004Tovit Lichi Across evolution, the signal recognition particle pathway targets extra-cytoplasmic proteins to membranous translocation sites. Whereas the pathway has been extensively studied in Eukarya and Bacteria, little is known of this system in Archaea. In the following, membrane association of FtsY, the prokaryal signal recognition particle receptor, and SRP54, a central component of the signal recognition particle, was addressed in the halophilic archaea Haloferax volcanii. Purified H. volcanii FtsY, the FtsY C-terminal GTP-binding domain (NG domain) or SRP54, were combined separately or in different combinations with H. volcanii inverted membrane vesicles and examined by gradient floatation to differentiate between soluble and membrane-bound protein. Such studies revealed that both FtsY and the FtsY NG domain bound to H. volcanii vesicles in a manner unaffected by proteolytic pretreatment of the membranes, implying that in Archaea, FtsY association is mediated through the membrane lipids. Indeed, membrane association of FtsY was also detected in intact H. volcanii cells. The contribution of the NG domain to FtsY binding in halophilic archaea may be considerable, given the low number of basic charges found at the start of the N-terminal acidic domain of haloarchaeal FtsY proteins (the region of the protein thought to mediate FtsY,membrane association in Bacteria). Moreover, FtsY, but not the NG domain, was shown to mediate membrane association of H. volcanii SRP54, a protein that did not otherwise interact with the membrane. [source] Prey switching in four species of carnivorous stonefliesFRESHWATER BIOLOGY, Issue 6 2004J. M. Elliott Summary 1. Previous studies compared the functional responses to their prey, and both intraspecific and interspecific interference, in mature larvae of Dinocras cephalotes, Perla bipunctata, Isoperla grammatica and Perlodes microcephalus. The present study examines switching by larvae of the same species presented with different proportions of two contrasting prey types; larvae of Baetis rhodani and Chironomus sp. In each experiment, 200 prey were arranged in nine different combinations of the two prey types (20 : 180, 40 : 160, 60 : 140, 80 : 120, 100 : 100, 120 : 80, 140 : 60, 160 : 40, 180 : 20). Prey were replaced as they were eaten. A model predicted the functional response in the absence of switching and provided a null hypothesis against which any tendency for switching could be tested. 2. No evidence for prey switching by Dinocras and Perla was obtained, both species showing a slight preference for Baetis over Chironomus. Prey switching occurred in Isoperla and Perlodes. As the relative abundance of one prey type increased in relation to the alternative, the proportion eaten of the former prey changed from less to more than expected from its availability, the relationship being described by an S-shaped curve. Isoperla and Perlodes switched to a preference for Baetis when its percentage of the total available prey exceeded 57 and 42%, respectively. Equivalent values for Chironomus were 43 and 58% for Isoperla and Perlodes, respectively. Switching was strongest in Perlodes. 3. Non-switching in Dinocras and Perla was related to their feeding strategy, both species being more successful when using a non-selective ambush strategy at dusk and dawn rather than a search strategy during the night. Both Isoperla and Perlodes used a search strategy. The smaller Isoperla fed chiefly at dusk and dawn, and preferred Chironomus larvae, whereas most of the larger Perlodes fed continuously from dusk to dawn and preferred Baetis larvae. [source] Sex-dependent use of information on conspecific feeding activities in an amphibian urodelianFUNCTIONAL ECOLOGY, Issue 2 2009Pedro Aragón Summary 1Animals can make decisions by observing the behaviour of others. Their decisions vary depending on whether the benefits of using such information exceed the costs. Thus, it is worthwhile to explore the potential costs associated with different sources of information to understand the consequences of sociality. Previous studies focused mainly on the costs inherent to information gathering, whereas sex differences with regard to the costs arising from information use have received less attention. 2To explore this further I performed two complementary experiments. The first experiment aimed to examine individual responses to different combinations of information types that are likely to appear in nature, to test whether there is a sex-dependent response in the Bosca's newt, Lissotriton boscai. I tested the time needed for individuals to eat food items by trial-and-error tactics (personal information), and when a conspecific, which was eating or not, was added to the food cue. The second experiment aimed to evoke social interactions that are likely to arise after the choice of using information on conspecific feeding activities is made. I examined the potential costs associated with direct competition between same-sex pairs in food-limited conditions. 3Results of experiment 1 revealed that in both sexes the latency to eat food items was shorter in the presence of non-feeding conspecifics, but only females took advantage when information related to feeding activities where added to the food cue. Results of experiment 2 showed that when faced with a limited resource of food, females were more prone to engage in costly interactions. 4This study suggests that the balance between costs and benefits associated with the short-term use of information on conspecific feeding behaviour in combination with food cues may differ between sexes. Differential information uses by sexes might have profound consequences in intraspecific relationships and in the evolution of vertebrates' social systems. [source] Effects of temperature and food quality on anuran larval growth and metamorphosisFUNCTIONAL ECOLOGY, Issue 5 2002D. Álvarez Summary 1Anurans exhibit high levels of growth-mediated phenotypic plasticity in age and size at metamorphosis. Although temperature and food quality exert a strong influence on larval growth, little is known about the interacting effects of these factors on age and size at metamorphosis. 2Plasticity in growth rates, maximum larval mass, mass loss, larval period and size at metamorphosis was examined in Iberian Painted Frogs (Discoglossus galganoi Capula, Nascetti, Lanza, Bullini & Crespo 1985) under different combinations of temperature and diet quality. 3Temperature and diet had strong effects on the maximum size reached by tadpoles throughout the premetamorphic stages. Larval body mass varied inversely with temperature. The effect of diet depended on temperature; larvae fed on a ,carnivorous' diet (rich in protein and lipids) achieved a larger size than larvae offered an ,herbivorous' diet (rich in carbohydrates) at 17 °C but not at 12 or 22 °C. 4Larval period was insensitive to diet composition, and varied only with temperature. Primarily the interacting effects of food quality and temperature affected size at metamorphosis. Size at metamorphosis varied inversely with temperature under the plant- and the animal-based diets. However, the carnivorous diet resulted in bigger metamorphs at 17 and 22 °C, but did not influence final mass at 12 °C. Maximum size over the larval period explained most of the variation in mass loss after the premetamorphic growing phase. [source] | |||||||||||||||||||||||||||||||||||||||||||||||||