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Current Observations (current + observation)
Selected AbstractsAmplification and overexpression of prosaposin in prostate cancerGENES, CHROMOSOMES AND CANCER, Issue 4 2005Shahriar Koochekpour We identified prosaposin (PSAP) as a secreted protein expressed in androgen-independent (AI) prostate cancer cells by cloning/sequencing, after probing a PC-3 cDNA library expressed in the ,TriplEx phagemid expression vector with a polyclonal rabbit antibody generated against pooled human seminal plasma. PSAP is a neurotrophic molecule; its deficiency or inactivation has proved to be lethal in man and mice, and in mice, it leads to abnormal development and atrophy of the prostate gland, despite normal testosterone levels. We used Southern hybridization, quantitative real-time polymerase chain reaction, and/or single nucleotide polymorphism (SNP) array analysis, and we now report the genomic amplification of PSAP in the metastatic AI prostate cancer cell lines, PC-3, DU-145, MDA-PCa 2b, M-12, and NCI-H660. In addition, by using SNP arrays and a set of 25 punch biopsy samples of human prostate cancer xenografts (LAPC3, LuCaP 23.1, 35, 49, 58, 73, 77, 81, 86.2, 92.1, 93, 96, 105, and 115), lymph nodes, and visceral-organ metastases, we detected amplification of the PSAP locus (10q22.1) in LuCaP 58 and 96 xenografts and two lymph node metastases. In addition, AI metastatic prostate cancer cell lines C4-2B and IA8-ARCaP over-expressed PSAP mRNA without evidence of genomic amplification. Taken together with prior data that demonstrated the growth-, migration-, and invasion-promoting activities, the activation of multiple signal transduction pathways, and the antiapoptotic effect of PSAP (or one of its active domains, saposin C) in prostate cancer cells, our current observation of PSAP amplification or overexpression in prostate cancer suggests, for the first time, a role for this molecule in the process of carcinogenesis or cancer progression in the prostate. © 2005 Wiley-Liss, Inc. [source] Documenting Loss of Large Trophy Fish from the Florida Keys with Historical PhotographsCONSERVATION BIOLOGY, Issue 3 2009LOREN McCLENACHAN arrecifes de coral; ecología histórica; directrices cambiantes; peces de arrecife; sobrepesca Abstract:,A loss of large vertebrates has occurred in aquatic and terrestrial ecosystems, but data to measure long-term population changes are sparse. Historical photographs provide visual and quantitative evidence of changes in mean individual size and species composition for groups of marine fish that have been targeted by sport fishing. I measured such trends for 13 groups of recreationally caught "trophy" reef fish with photographs taken in Key West, Florida, from 1956 to 2007. The mean fish size declined from an estimated 19.9 kg (SE 1.5) to 2.3 kg (SE 0.3), and there was a major shift in species composition. Landings from 1956 to 1960 were dominated by large groupers (Epinephelus spp.), and other large predatory fish were commonly caught, including sharks with an average length of just <2 m. In contrast, landings in 2007 were composed of small snappers (Lutjanus spp. and Ocyurus chrysurus) with an average length of 34.4 cm (SE 0.62), and the average length of sharks declined by more than 50% over 50 years. Major declines in the size of fish caught were not reflected in the price of fishing trips, so customers paid the same amount for a less-valuable product. Historical photographs provide a window into a more pristine coral reef ecosystem that existed a half a century ago and lend support to current observations that unfished reef communities are able to support large numbers of large-bodied fish. Resumen:,Una pérdida de vertebrados mayores ha ocurrido en ecosistemas acuáticos y terrestres, pero los datos para medir los cambios poblaciones a largo plazo son escasos. Las fotografías históricas proporcionan evidencia visual y cuantitativa de cambios en el tamaño individual promedio y de la composición de especies en grupos de peces marinos que han sido blanco de la pesca deportiva. Medí esas tendencias en 13 grupos de peces de arrecife capturados recreativamente como "trofeos" mediante fotografías tomadas en Key West, Florida, desde 1956 a 2007. El peso promedio de los peces declinó de unos 19.9 kg (ES 1.5) a 2.3 kg (ES 0.3), y hubo un cambio mayor en la composición de especies. Las capturas entre 1956 y 1960 estuvieron dominadas por meros (Epinephelus spp.) grandes, y otros peces depredadores eran capturados comúnmente, incluyendo tiburones con una longitud promedio de poco menos de 2m. En contraste, las capturas en 2007 fueron compuestas de pargos (Lutjanus spp. y Ocyurus chrysurus) pequeños con una longitud promedio de 34.4 cm (ES 0.62), y la longitud promedio de los tiburones declinó más de 50% en 50 años. La gran declinación en el tamaño de los peces capturados no se reflejó en los precios de los viajes de pesca, así que los clientes pagaron la misma cantidad por un producto menos valioso. Las fotografías históricas proporcionan una visión de un ecosistema arrecifal coralino prístino que existió hace medio siglo y proporcionan soporte a los comentarios actuales de que las comunidades arrecifales no explotadas son capaces de soportar numerosos peces de talla grande. [source] Neuropathology of Rett syndromeDEVELOPMENTAL DISABILITIES RESEARCH REVIEW, Issue 2 2002Dawna Duncan Armstrong Abstract Rett Syndrome is unlike any other pediatric neurologic disease, and its clinical-pathologic correlation can not be defined with standard histology techniques. Based on hypotheses suggested by careful clinical observations, the nervous system of the Rett child has been explored utilizing morphometry, golgi preparations, computerized tomography, magnetic resonance imaging, chemistry, immunocytochemistry, autoradiography, and molecular biologic techniques. From these many perspectives we conclude that Rett syndrome is not a typical degenerative disorder, storage disorder, nor the result of gross malformation, infectious or neoplastic processes. There remain regions of the brain that have not been studied in detail but the available data suggest that the neuropathology of Rett syndrome can be summarized as follows: the Rett brain is small for the age and the height of the patient; it does not become progressively smaller over three to four decades; it has small dendritic trees in pyramidal neurons of layers III and V in selected lobes (frontal, motor, and temporal); it has small neurons with an increased neuronal packing density; it has an immature expression of microtubular protein-2 and cyclooxygenase; it exhibits a changing pattern of neurotransmitter receptors with an apparent reduction in many neurotransmitters, possibly contributing to some symptomatology. A mutation in Mecp2 causes this unique disorder of brain development. Neuronal mosaicism for normal and mutated Mecp2 produces a consistent phenotype in the classic female patient and a small brain with some preserved islands of function, but with an inability to support hand use and speech. This paper summarizes our current observations about neuropathology of Rett syndrome. MRDD Research Reviews 2002;8:72,76. © 2002 Wiley-Liss, Inc. [source] Observations at terrestrial impact structures: Their utility in constraining crater formationMETEORITICS & PLANETARY SCIENCE, Issue 2 2004Richard A. F. Grieve Local geology of the target area tends to be of secondary importance, and the net result is that impacts of similar size on a given planetary body produce similar results. This is the essence of the utility of observations at impact craters, particularly terrestrial craters, in constraining impact processes. Unfortunately, there are few well-documented results from systematic contemporaneous campaigns to characterize specific terrestrial impact structures with the full spectrum of geoscientific tools available at the time. Nevertheless, observations of the terrestrial impact record have contributed substantially to fundamental properties of impact. There is a beginning of convergence and mutual testing of observations at terrestrial impact structures and the results of modeling, in particular from recent hydrocode models. The terrestrial impact record provides few constraints on models of ejecta processes beyond a confirmation of the involvement of the local substrate in ejecta lithologies and shows that Z-models are, at best, first order approximations. Observational evidence to date suggests that the formation of interior rings is an extension of the structural uplift process that occurs at smaller complex impact structures. There are, however, major observational gaps and cases, e.g., Vredefort, where current observations and hydrocode models are apparently inconsistent. It is, perhaps, time that the impact community as a whole considers documenting the existing observational and modeling knowledge gaps that are required to be filled to make the intellectual breakthroughs equivalent to those of the 1970s and 1980s, which were fueled by observations at terrestrial impact structures. Filling these knowledge gaps would likely be centered on the later stages of formation of complex and ring structures and on ejecta. [source] An analysis of the factory model for chromosome replication and segregation in bacteriaMOLECULAR MICROBIOLOGY, Issue 4 2001James Sawitzke Recent advances in microscopy have given us important clues as to the nature of chromosome segregation in bacteria. Most current observations favour the view that the process is co-replicational: DNA replication forks are anchored at the cell centre, and the newly replicated DNA is moved towards the cell poles. This scheme can account for orderly segregation even at high growth rates where multiple replication cycles overlap. We argue that there are five distinct activities directly involved in co-replicational segregation dynamics. These we refer to as Push, Direct, Condense, Hold and Clear. We attempt to assign one of these roles to each protein implicated in chromosome segregation. The proposed process is very different from mitosis in eukaryotic cells and perhaps more closely resembles the formation of separate sister chromatids during DNA replication. [source] What is the largest Einstein radius in the universe?MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2009Masamune Oguri ABSTRACT The Einstein radius plays a central role in lens studies as it characterizes the strength of gravitational lensing. In particular, the distribution of Einstein radii near the upper cut-off should probe the probability distribution of the largest mass concentrations in the universe. Adopting a triaxial halo model, we compute expected distributions of large Einstein radii. To assess the cosmic variance, we generate a number of Monte Carlo realizations of all-sky catalogues of massive clusters. We find that the expected largest Einstein radius in the universe is sensitive to parameters characterizing the cosmological model, especially ,8: for a source redshift of unity, they are 42+9,7, 35+8,6 and 54+12,7 arcsec (errors denote 1, cosmic variance), assuming best-fitting cosmological parameters of the Wilkinson Microwave Anisotropy Probe five-year (WMAP5), three-year (WMAP3) and one-year (WMAP1) data, respectively. These values are broadly consistent with current observations given their incompleteness. The mass of the largest lens cluster can be as small as , 1015 M,. For the same source redshift, we expect in all sky ,35 (WMAP5), ,15 (WMAP3) and ,150 (WMAP1) clusters that have Einstein radii larger than 20 arcsec. For a larger source redshift of 7, the largest Einstein radii grow approximately twice as large. Whilst the values of the largest Einstein radii are almost unaffected by the level of the primordial non-Gaussianity currently of interest, the measurement of the abundance of moderately large lens clusters should probe non-Gaussianity competitively with cosmic microwave background experiments, but only if other cosmological parameters are well measured. These semi-analytic predictions are based on a rather simple representation of clusters, and hence calibrating them with N -body simulations will help to improve the accuracy. We also find that these ,superlens' clusters constitute a highly biased population. For instance, a substantial fraction of these superlens clusters have major axes preferentially aligned with the line-of-sight. As a consequence, the projected mass distributions of the clusters are rounder by an ellipticity of ,0.2 and have , 40,60 per cent larger concentrations compared with typical clusters with similar redshifts and masses. We argue that the large concentration measured in A1689 is consistent with our model prediction at the 1.2, level. A combined analysis of several clusters will be needed to see whether or not the observed concentrations conflict with predictions of the flat ,-dominated cold dark matter model. [source] The lepton asymmetry: the last chance for a critical-density cosmology?MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2001Julien Lesgourgues We use a wide range of observations to constrain cosmological models possessing a significant asymmetry in the lepton sector, which offer perhaps the best chance of reconciling a critical-density Universe with current observations. The simplest case, with massless neutrinos, fails to fit many experimental data and does not lead to an acceptable model. If the neutrinos have mass of order 1 eV (which is favoured by some neutrino observations), then models can be implemented which prove a good fit to the microwave anisotropies and large-scale structure data. However, taking into account the latest microwave anisotropy results, especially those from BOOMERANG, we show that the model can no longer accommodate the observed baryon fraction in clusters. Together with the observed acceleration of the present Universe, this puts considerable pressure on such critical-density models. [source] Kaolin polytypes revisited ab initioACTA CRYSTALLOGRAPHICA SECTION B, Issue 2 2008Patrick H. J. Mercier The well known 36 distinguishable transformations between adjacent kaolin layers are split into 20 energetically distinguishable transformations (EDT) and 16 enantiomorphic transformations, hereafter denoted EDT*. For infinitesimal energy contribution of interactions between non-adjacent layers, the lowest-energy models must result from either (a) repeated application of an EDT or (b) alternate application of an EDT and its EDT*. All modeling, quantum input preparation and interpretation was performed with Materials Toolkit, and quantum optimizations with VASP. Kaolinite and dickite are the lowest-energy models at zero temperature and pressure, whereas nacrite and HP-dickite are the lowest-enthalpy models under moderate pressures based on a rough enthalpy/pressure graph built from numbers given in the supplementary tables. Minor temperature dependence of this calculated 0,K graph would explain the bulk of the current observations regarding synthesis, diagenesis and transformation of kaolin minerals. Other stackings that we list have energies so competitive that they might crystallize at ambient pressure. A homometric pair of energetically distinguishable ideal models, one of them for nacrite, is exposed. The printed experimental structure of nacrite correctly corresponds to the stable member of the pair. In our opinion, all recent literature measurements of the free energy of bulk kaolinite are too negative by ,,15,kJ,mol,1 for some unknown reason. [source] Suboptimal filter for continuous-time linear systems with unknown parametersASIAN JOURNAL OF CONTROL, Issue 5 2008Du Yong Kim Abstract The filtering problem for continuous-time linear systems with unknown parameters is considered. A new suboptimal filter is herein proposed. It is based on the optimal mean-square linear combination of the local Kalman filters. In contrast to the optimal weights, the suboptimal weights do not depend on current observations; thus, the proposed filter can easily be implemented in real-time. Examples demonstrate high accuracy and efficiency of the suboptimal filter. Copyright © 2008 John Wiley and Sons Asia Pte Ltd and Chinese Automatic Control Society [source] | |||||||||||||