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Cultivation
Kinds of Cultivation Terms modified by Cultivation Selected AbstractsAN INTRODUCTION TO THE CLIMBING DICENTRAS , THE GENUS DACTYLICAPNOS IN CULTIVATIONCURTIS'S BOTANICAL MAGAZINE, Issue 3 2008Nicholas Hind Summary The genus Dactylicapnos Wall. (Papaveraceae: Fumarioideae: Corydaleae) consists of annual and perennial climbers with half-hardy stems, tendrils on the leaves and, in most species, perennating, tuberous rootstocks. A short history of the genus is given here and its status is discussed. At present four species appear to be in cultivation and cultivation notes for these are provided. A description of the genus is given together with a synopsis, or nomenclator, of the species and associated names. [source] Oxygen and Temperature Control during the Cultivation of Microorganisms using Substrate FeedingENGINEERING IN LIFE SCIENCES (ELECTRONIC), Issue 3 2007J. Vanags Aerobic fermentation via substrate feeding controlled by O2 and temperature supplements the tools for the experimental and possibly industrial use. This substrate-feeding algorithm was realized with the help of the flexible Bioprocess controller BIO-2. [source] Cultivation of the Dible Mushroom Lentinula edodes (Shiitake) in Pasteurized Wheat Straw , Alternative Use of Georthermal Energy in MexicoENGINEERING IN LIFE SCIENCES (ELECTRONIC), Issue 4 2004G. Mata Abstract Five edible Lentinula edodes strains were evaluated. The mushrooms were cultivated on a wheat straw substrate that was previously pasteurized by immersion in water heated by residual geothermal vapor, which was also used to warm incubation and production rooms. Finely chopped wheat straw (Triticum aestivum L.) was pasteurized and then spawned with supplemented spawn capable of supplying nutrients and enriching the substrate, with the expectation of yield improvement. The samples were incubated for 60,days before the production started and thus, the mushrooms produced had pileus diameters ranging from 5 to 20,cm. The yields fluctuated from 6.2 to 13.9,% (fresh weight of mushrooms/fresh weight of substrate). Biological efficiency ranged from 24.8 to 55.6,% (fresh weight of mushrooms/dry weight of substrate), while the production rate reached varied from 0.19 to 0.55,% (biological efficiency/production time starting from inoculation). The cultivation system evaluated here offers the possibility of lowering production costs by cultivating the mushroom on easily obtainable substrate and shortening the culture cycle. The efficiency of this use of geothermal energy and supplemented spawn for shiitake mushroom cultivation on non-sterilized substrates was proven. [source] Assessment of the resistance of potato cultivars to Synchytrium endobioticum (Schilb.) Per. in PolandEPPO BULLETIN, Issue 2 2008J. Przetakiewicz In Poland the Plant Breeding and Acclimatization Institute is responsible for officially assessing the resistance to Synchytrium endobioticum of domestic potato breeding lines and cultivars from other countries. Cultivation of potato cultivars in Poland requires confirmation of resistance to potato wart disease. The official assessment uses the modified Glynne-Lemmerzahl method (laboratory tests) and pot tests. The full cycle of assessment of resistance to wart disease requires 52 seed potatoes per variety/breeding line. Forty two tubers are used in laboratory tests. To complete the laboratory tests the next 10 tubers are grown in pot tests (in soil with winter sporangia) during the vegetation season. The final results for domestic breeding lines of potato are available after 3 years of investigation. For cultivars from other countries the authorization of resistance to S. endobioticum takes approximately one year. The Polish breeders (breeding lines) or the breeder's representative (cultivars from other countries) receive the certificate only for lines/cultivars with laboratory and field resistance to S. endobioticum. [source] Screening for resistance to Didymella bryoniae in rootstocks of melonEPPO BULLETIN, Issue 2 2000P. Trionfetti Nisini Glasshouse evaluations of rootstocks of melon for resistance to Didymella bryoniae were conducted on different cucurbits previously selected as resistant or partially resistant to race 1,2 of Fusarium oxysporum f. sp. melonis. Cucumis anguria, C. ficifolius, C. figarei, C. metuliferus, C. zeyheri and Benincasa hispida showed a very high degree of resistance to D. bryoniae both on leaves and stems. Among the commercial rootstocks, Cucurbita hybrids ELSI, ES 99-13, RS 841, displayed a similar level of resistance. Stem inoculation of three cucurbit species grafted with susceptible melon cv. Proteo determined the occurrence of limited symptoms, but the lesions remained confined in the rootstock not affecting the grafted plant. Cultivation of susceptible melon cultivars grafted on resistant rootstocks may represent an efficient method for controlling Didymella crown rot and Fusarium wilt. [source] Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygenFEMS MICROBIOLOGY ECOLOGY, Issue 3 2006Ingeborg Bussmann Abstract In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at a minimum concentration at the layer of consumption. Opposing gradients of methane and oxygen were mimicked in a specific cultivation set-up in which growth of methanotrophic bacteria occurred as a sharp band at either c. 5 or 20 mm below the air-exposed end. Two new strains of methanotrophic bacteria were isolated with this system. One isolate, strain LC 1, belonged to the Methylomonas genus (type I methantroph) and contained soluble methane mono-oxygenase. Another isolate, strain LC 2, was related to the Methylobacter group (type I methantroph), as determined by 16S rRNA gene and pmoA sequence similarities. However, the partial pmoA sequence was only 86% related to cultured Methylobacter species. This strain accumulated significant amounts of formaldehyde in conventional cultivation with methane and oxygen, which may explain why it is preferentially enriched in a gradient cultivation system. [source] The management of arable land from prehistory to the present: Case studies from the Northern Isles of ScotlandGEOARCHAEOLOGY: AN INTERNATIONAL JOURNAL, Issue 1 2006Erika B. Guttmann The arable soils from two multiperiod settlements were analyzed to identify changes in agricultural methods over time. The settlement middens were also analyzed to determine whether potential fertilizers were discarded unused. Results suggest that in the Neolithic period (,4000,2000 B.C. in the UK) the arable soils at Tofts Ness, Orkney, and Old Scatness, Shetland, were created by flattening and cultivating the settlements' midden heaps in situ. The arable area at Tofts Ness was expanded in the Bronze Age (,2000,700 B.C. in the UK), and the new land was improved by the addition of ash, nightsoil, and domestic waste. Cultivation continued briefly after the fields were buried in windblown sand in the Late Bronze Age or Early Iron Age, but by the Early Iron Age cultivation ceased and organic-rich material was allowed to accumulate within the settlement. By contrast, at Old Scatness, arable production was increased in the Iron Age (,700 B.C.,A.D. 550 in Scotland) by the intensive use of animal manures. The results indicate that during the lifespan of the two settlements the arable soils were fertilized to increase production, which was intensified over time. © 2006 Wiley Periodicals, Inc. [source] Erosion and Nutrient Loss on Sloping Land under Intense Cultivation in Southern VietnamGEOGRAPHICAL RESEARCH, Issue 1 2008NGUYEN VAN DE Abstract To help improve the well-being of the local people, a joint Vietnamese-UK team set out to establish a way of estimating soil and nutrient losses under different land management scenarios, using field data extrapolated through remote sensing and GIS, to obtain catchment-wide estimates of the impact of land cover change. Immigration from remote provinces to the Dong Phu District of Binh Phuóc Province, about 120 km north of Ho Chi Minh City, has led to disruption of soil surface stability on easily eroded clayey sandstones, creating rapid nutrient depletion that affects crop yields and siltation in the channel of the Rach Rat river downstream. The poor farmers of the areas see crop yields drop dramatically after two or three years of cultivation due to the fertility decline. Soil loss varies dramatically between wet season and dry season and with ground cover. Erosion bridge measurements showed a mean loss of 85.2 t ha,1 y,1 under cassava saplings with cashew nuts, 43.3 t ha,1 y,1 on uncultivated land and 41.7 t ha,1 y,1 under mature cassava. The rates of erosion were higher than those reported in many other parts of Vietnam, reflecting the high erodibility of the friable sandy soils on the steep side-slopes of the Rach Rat catchment. However, although the actual measurements provide better soil loss data than estimates based on the parameters of soil loss equations, a large number of measurement sites is needed to provide adequate coverage of the crop and slope combinations in this dissected terrain for good prediction using GIS and remote sensing. [source] Cultivation and ensilage of yacon plants (Smallanthus sonchifolius [Poepp.GRASSLAND SCIENCE, Issue 1 2009& Endl.] H. Robinson), the function of yacon silage Abstract Yacon (Smallanthus sonchifolius [Poepp. & Endl.] H. Robinson) plant cultivation has extended rapidly throughout Japan since 1985 because of its utility as a tuberous root crop. In this study, strains with high yields of both above-ground and underground parts were identified to investigate the use of above-ground parts for animal feed silage. In addition, we measured the anti-oxidative activity of the silage as a functional characteristic. Data from 5 years of the cultivation of 17 yacon strains suggest that the most suitable strain for planting in the Aso region of Japan is SY217, which has a high yield of both above-ground parts and tuberous roots. The root tuber parts of yacon are used almost entirely as food; actually, most foliage parts are hardly used, except for some above-ground parts, which are used as tea. The fermentation qualities of the silage using all above-ground parts and harvest residue tuberous roots (harvest residues) are high and their dry matter digestibility, investigated using pigs, is 66.7%. Fresh above-ground parts had very strong anti-oxidative activity as compared with butylated hydroxyanisole and ,-tocopherol. In addition, that activity is retained during ensiling for 4 weeks. These results suggest that the entire yacon plant can be used effectively as a feedstuff resource with the added bonus of anti-oxidant activity. [source] Cultivation and engineering of a software metrics programINFORMATION SYSTEMS JOURNAL, Issue 1 2003Jakob Iversen Abstract. This paper reports from a case study of an organization that implements a software metrics program to measure the effects of its improvement efforts. The program measures key indicators of all completed projects and summarizes progress information in a quarterly management report. The implementation turns out to be long and complex, as the organization is confronted with dilemmas based on contradictory demands and value conflicts. The process is interpreted as a combination of a rational engineering process in which a metrics program is constructed and put into use, and an evolutionary cultivation process in which basic values of the software organization are confronted and transformed. The analysis exemplifies the difficulties and challenges that software organizations face when bringing known principles for software metrics programs into practical use. The article discusses the insights gained from the case in six lessons that may be used by Software Process Improvement managers in implementing a successful metrics program. [source] Consumer attitudes and acceptance of genetically modified organisms in KoreaINTERNATIONAL JOURNAL OF CONSUMER STUDIES, Issue 3 2003Hyochung Kim Genetically modified organisms (GMOs) were first used to designate micro organisms that had had genes from other species transferred into their genetic material by the then-new techniques of ,gene-splicing.' Cultivation of GMOs has so far been most widespread in the production of soybeans and maize. The United States holds almost three-fourths of the total crop area devoted to GMOs. Because many crops have been imported from the US, there is a large possibility for consumers to intake the products of GMOs in Korea. The safety of GMOs is not scientifically settled at this time, however. Additionally, the research regarding the GMOs issue of consumers has rarely been conducted in Korea. This study therefore focused on the consumer attitudes about GMOs and willingness to purchase them. The data were collected from 506 adults living in Seoul, Daegu and Busan, Korea, by means of a self-administered questionnaire. Frequencies and chi-square tests were conducted by SPSS. The results of the survey were as follows. First, the consumer concerns about GMOs were high but recognition was low; many respondents answered they did not have exact information about GMOs, although they had heard about them. Second, almost 93% of the respondents desired the labelling of GMOs. Third, the level of acceptance of GMOs was high; two-thirds of the respondents showed that they were willing to buy GMOs. Finally, many respondents worried about the safety of GMOs in that 73% of the respondents primarily wanted to be informed about safety of GMOs. This study suggests that the consumer education about GMOs should be conducted through mass media and consumer protection organisations. [source] Determinants of Voter Support for a Five-Year Ban on the Cultivation of Genetically Modified Crops in SwitzerlandJOURNAL OF AGRICULTURAL ECONOMICS, Issue 3 2008Felix Schläpfer D62; D72; Q26 Abstract While much effort has been devoted to estimating market premiums for non-genetically modified (GM) food, the results of such research are largely silent about the preferences for the public good aspects, or externalities, of GM food production. For public goods, the closest substitute of private consumption decisions is voting on referenda. In November 2005, 55.7% of 2 million Swiss voters approved a five-year moratorium (ban) on the commercial cultivation of GM plants in Switzerland. The present study examines how individual voting decisions were determined by: (i) socioeconomic characteristics; (ii) political preference/ideology; and (iii) agreement with a series of arguments in favour and against the use of GM plants in Swiss agriculture. The analysis is based on the data of the regular voter survey undertaken after the national-level voting in Switzerland. The results suggest that current concerns about the use of genetically engineered plants in agriculture may not automatically decrease with higher levels of education/knowledge and generational change. Furthermore, analysis of voter motives suggests that public support for a ban on GM crops may be even larger in other countries, where industrial interests in crop biotechnology are less pronounced. [source] Yield, Boll Distribution and Fibre Quality of Hybrid Cotton (Gossypium hirsutum L.) as influenced by Organic and Modern Methods of CultivationJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 4 2006D. Blaise Abstract India is the largest cotton-growing country (8.9 million hectares) in the world and most of the area is rain-dependent. Large amount of pesticides are used for the control of sucking pests and lepidopterans. Increasing demand for clean organic fibre has led to an interest in organic cotton. However, information on the effects of organic cultivation on fibre quality is limited. Seed cotton yield and fibre quality (length, strength, micronaire and uniformity) were determined for an organic and modern method of cultivation during 3 years (2002,2003 to 2004,2005) of a 11-year (1994,1995 to 2004,2005) study. Vertical and horizontal distribution of bolls on a cotton plant was also determined in 2003,2004 and 2004,2005. At the end of year 11, soil samples were collected and analysed for soil organic carbon content, water-stable aggregates (%), and mean weight diameter. Averaged over 3 years, an additional 94 kg seed cotton ha,1 was produced in the organic over the modern method of cultivation and the difference was significant. The year × treatment interaction was significant. Seed cotton yield in the organic plots was significantly greater than the modern method of cultivation plots in 2003,2004 because of a well-distributed normal rainfall and low pest incidence. The main stem nodes 13,22 accounted for the largest numbers of bolls present on the plant. Plants of the organic plots had significantly (37,71 %) more bolls on nodes 13,27 than those for the plants of the modern method of cultivation. Lateral distribution of bolls on a sympodial (fruiting) branch, was noticed up to fruiting point 11. However, treatment differences were not significant. With regard to fibre quality (length, strength, fineness and uniformity), differences between years were significant. Inferior quality fibre was produced in 2004,2005 because of delayed planting and early cessation of rain. On average, cotton grown under organic conditions compared with the modern method of cultivation had significantly better fibre length (25.1 vs. 24.0 mm) and strength (18.8 vs. 17.9 g tex,1). Soil samples of the organic plots had significantly greater C content, water-stable aggregates and mean weight diameter than the modern method of cultivation plots. Differences were restricted to the top layers (0,0.1 and 0.1,0.2 m). Yield benefits of growing cotton in an organic system over the modern method of cultivation are expected to be greater in years receiving normal rainfall and having low pest incidence. [source] Assessment of the rind microbial diversity in a farmhouse-produced vs a pasteurized industrially produced soft red-smear cheese using both cultivation and rDNA-based methodsJOURNAL OF APPLIED MICROBIOLOGY, Issue 3 2004C. Feurer Abstract Aims:, The diversity of the surface flora of two French red-smear soft cheeses was examined by cultivation-dependent and cultivation-independent methods to assess their composition and to evaluate the accuracy of both approaches. Methods and Results:, Culture-independent methods used involved 16S ribosomal DNA gene cloning and sequencing and single-strand conformation polymorphism analysis (SSCP). The culture-dependent method used involved direct culture and macroscopic observation, polymerase chain reaction of the 16S rRNA gene from DNA extracted from single colonies followed by complete sequencing of the gene. Only few species were recovered by both approaches either in the pasteurized and the farmer cheese. A large diversity of isolates or 16S rDNA sequences related to marine bacteria was identified at the surface of both cheeses. Conclusions:, The results indicated that all three techniques were informative and complementary to allow a more accurate representativeness of the cheese surface biodiversity. Significance and Impact of the Study:, Cultivation and molecular methods have to be combined in order to obtain an extended view of the bacterial populations of complex ecosystems. [source] The Scary World in Your Living Room and Neighborhood: Using Local Broadcast News, Neighborhood Crime Rates, and Personal Experience to Test Agenda Setting and CultivationJOURNAL OF COMMUNICATION, Issue 3 2003Kimberly Gross This study tested 2 important theories in the history of mass communication research, agenda setting and cultivation, by comparing the effects of watching local television news with direct experience measures of crime on issue salience and fear of victimization. Direct experience was measured in 2 ways: (a) personal crime victimization or victimization of a close friend or family member, and (b) neighborhood crime rates. Using a random digit dial telephone survey of residents of the Washington, DC, metropolitan area, researchers found that local news exposure accounted for an agenda-setting effect but did not cultivate fear of being a victim of crime. By contrast, direct experience had no agenda-setting effect but did predict fear. [source] Yield of Illicit Indoor Cannabis Cultivation in The NetherlandsJOURNAL OF FORENSIC SCIENCES, Issue 5 2006Marcel Toonen Ph.D. ABSTRACT: To obtain a reliable estimation on the yield of illicit indoor cannabis cultivation in The Netherlands, cannabis plants confiscated by the police were used to determine the yield of dried female flower buds. The developmental stage of flower buds of the seized plants was described on a scale from 1 to 10 where the value of 10 indicates a fully developed flower bud ready for harvesting. Using eight additional characteristics describing the grow room and cultivation parameters, regression analysis with subset selection was carried out to develop two models for the yield of indoor cannabis cultivation. The median Dutch illicit grow room consists of 259 cannabis plants, has a plant density of 15 plants/m2, and 510 W of growth lamps per m2. For the median Dutch grow room, the predicted yield of female flower buds at the harvestable developmental stage (stage 10) was 33.7 g/plant or 505 g/m2. [source] Cultivation of flax in spoil-bank clay: Mycorrhizal inoculation vs. high organic amendmentsJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 6 2008David Püschel Abstract A greenhouse experiment was focused on the application of arbuscular mycorrhizal fungi (AMF) in effective crop production during reclamation of coal-mine spoil banks. The aim of the study was to find out whether mycorrhizal inoculation improves growth of flax (Linum usitatissimum L.) and whether it can compensate for high doses of expensive organic amendment. Flax was planted in original spoil-bank gray Miocene clay amended with organic matter used for spoil-bank reclamation (mixture of composted urban waste and lignocellulose papermill waste). This amendment was applied in four descending doses equivalent to the application of 500, 200, 100, and 0 t ha,1. The plants received either a mixed inoculum of three AMF species (Glomus mosseae, G. claroideum, and G. intraradices) or were left uninoculated. Growth of flax was significantly increased by mycorrhizal inoculation in pure clay (by 60%) as well as in clay with all amendment doses (by 119% on average). Also, the addition of organic matter increased plant growth but, interestingly, the biomass production was comparable regardless the dose of amendment. Our results suggest that similar yields are attainable with only one fifth of the usual fertilization dose, which can significantly decrease costs related to the reclamation of spoil banks. If mycorrhizal inoculation is applied together with the optimized fertilization, growth of flax planted in spoil-bank clay can be further improved. [source] Cichorium intybus L , cultivation, processing, utility, value addition and biotechnology, with an emphasis on current status and future prospectsJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 5 2001Harsh Pal Bais Abstract Cultivation of chicory plants for various applications, such as utilisation of the root biomass for preparation of a coffee adjuvant, utilisation as a vegetable and, recently, utility of the plants for important phytochemicals, has received global attention. Chicory is widely grown in countries of different geographical locations owing to the economic importance of this crop. This review addresses cultivation, utility, phytochemical studies and pharmacological aspects, with an emphasis on biotechnological developments in recent years and safety evaluation of genetically modified chicory crops. These aspects are dealt with in detail to bring out the current status and future prospects of cultivation and utility of this economically important crop. © 2001 Society of Chemical Industry [source] Cultivation of medicinal isabgol (Plantago ovata) in alkali soils in semiarid regions of Northern IndiaLAND DEGRADATION AND DEVELOPMENT, Issue 3 2006J. C. Dagar Abstract There is growing global demand for medicinal drugs including isabgol (Plantago ovata). With increasing demand of food for an ever-increasing population in India, it is not possible to bring arable lands under cultivation for aromatic and medicinal plants. Salt-affected lands (both saline and alkali) occupy about 8·6 million ha. Due to poor physical properties and excessive exchangeable Na+, most of these lands do not support good vegetation cover. The marginal and salt-affected lands could be successfully utilized for the cultivation of aromatic and medicinal plants. We achieved almost complete germination of isabgol seeds using up to 5000,ppm salt-solution. Grain yield (including husk) was 1·47 to 1·58,t,ha,1 at pH 9·2 showing no significant yield reduction as compared to normal soil. At pH 9·6 the grain yield was 1·03 to 1·12,t,ha,1. At higher pH there was significant reduction in yield. Sowing in good moisture (at field capacity) of soil was found best, but to save time sowing at shallow depth in dry soil, followed by irrigation was also suitable as compared to broadcasting seeds. The chlorophyll content was greater 70 days after sowing compared to younger stages (50 days after sowing). The total chlorophyll and plant biomass were lower from crops grown by broadcasting methods of sowing as compared to two other methods of sowing. The leaf area index (LAI) was higher for the broadcasting method of sowing as compared to the other two methods. Na+ absorption increased and K+ and K+/Na+ ratio decreased with increase in pH. Results reported in this paper clearly indicate that isabgol can successfully be grown on moderately alkali soils up to pH 9·6 without the application of any amendment. Copyright © 2006 John Wiley & Sons, Ltd. [source] Cultivation of low-temperature (15°C), anaerobic, wastewater treatment granulesLETTERS IN APPLIED MICROBIOLOGY, Issue 4 2009J. O'Reilly Abstract Aims:, Anaerobic sludge granules underpin high-rate waste-to-energy bioreactors. Granulation is a microbiological phenomenon involving the self-immobilization of several trophic groups. Low-temperature anaerobic digestion of wastes is of intense interest because of the economic advantages of unheated bioenergy production technologies. However, low-temperature granulation of anaerobic sludge has not yet been demonstrated. The aims of this study were to (i) investigate the feasibility of anaerobic sludge granulation in cold (15°C) bioreactors and (ii) observe the development of methanogenic activity and microbial community structure in developing cold granules. Methods and Results:, One mesophilic (R1; 37°C) and two low-temperature (R2 and R3, 15°C) laboratory-scale, expanded granular sludge bed bioreactors were seeded with crushed (diameter <0·4 mm) granules and were fed a glucose-based wastewater for 194 days. Bioreactor performance was assessed by chemical oxygen demand removal, biogas production, granule growth and temporal methanogenic activity. Granulation was observed in R2 and R3 (up to 33% of the sludge). Elevated hydrogenotrophic methanogenesis was observed in psychrophilically cultivated biomass, but acetoclastic methanogenic activity was also retained. Denaturing gradient gel electrophoresis of archaeal 16S rRNA gene fragments indicated that a distinct community was associated with developing and mature granules in the low-temperature (LT) bioreactors. Conclusions:, Granulation was observed at 15°C in anaerobic bioreactors and was associated with H2/CO2 -mediated methanogenesis and distinct community structure development. Significance and Impact of the Study:, Granulation underpins high-rate anaerobic waste treatment bioreactors. Most LT bioreactor trials have employed mesophilic seed sludge, and granulation <20°C was not previously documented. [source] Bifidobacterium and Lactobacillus DNA in the human placentaLETTERS IN APPLIED MICROBIOLOGY, Issue 1 2009R. Satokari Abstract Aims:, Bifidobacteria and lactobacilli are part of the human normal intestinal microbiota and may possibly be transferred to the placenta. It was hypothesized that intestinal bacteria or their components are present in the placenta and that the foetus may be exposed to them. We investigated the presence of bifidobacteria and lactobacilli and their DNA in the human placenta. Methods and Results:, We studied 34 human placentae (25 vaginal and nine caesarean deliveries) for the presence Bifidobacterium spp. and Lactobacillus rhamnosus. Cultivation was used for the detection of viable cells and genus and species-specific PCR for the detection of DNA. No bifidobacteria or lactobacilli were found by cultivation. Bifidobacterial DNA was detected in 33 and L. rhamnosus DNA in 31 placenta samples. Conclusions:, DNA from intestinal bacteria was found in most placenta samples. The results suggest that horizontal transfer of bacterial DNA from mother to foetus may occur via placenta. Significance and Impact of the Study:, Bacterial DNA contains unmethylated CpG oligodeoxynucleotide motifs which induce immune effects. Specific CpG motifs activate Toll-like receptor 9 and subsequently trigger Th-1-type immune responses. Although the newborn infant is considered immunologically immature, exposure by bacterial DNA may programme the infant's immune development during foetal life earlier than previously considered. [source] Planting the Nation's ,Waste Lands': Walter Scott, Forestry and the Cultivation of Scotland's WildernessLITERATURE COMPASS (ELECTRONIC), Issue 3 2009Susan Oliver In October 1827 the Quarterly Review included a review of the second edition of Robert Monteath's The Planter's Guide and Profitable Planter. The review was published anonymously according to custom, but the author was Sir Walter Scott. A keen amateur plantsman who would later be involved in producing official reports on tree husbandry in Scotland, Scott's interest in ecology, forestry and the cultural value of landscape was of long standing. He had spent a small fortune on trees for his Abbotsford estate, and the cost had contributed to his insolvency in 1826. The present article looks at Scott's review as a work of Romantic ecocriticism concerned with the relationships between nationhood, economics and natural sustainability. Definitions of ,waste land' are considered, and the use of literary references to emphasize the need for sustainable planting is explored along with debates over imported Canadian species of pine. The cultural exchange of trees for people is shown to raise interesting questions, as is the advent of the railways that Scott ignores in his essay despite his interest in that new form of transport. [source] Isolation and characterization of Cupriavidus basilensis HMF14 for biological removal of inhibitors from lignocellulosic hydrolysateMICROBIAL BIOTECHNOLOGY, Issue 3 2010Nick Wierckx Summary The formation of toxic fermentation inhibitors such as furfural and 5-hydroxy-2-methylfurfural (HMF) during acid (pre-)treatment of lignocellulose, calls for the efficient removal of these compounds. Lignocellulosic hydrolysates can be efficiently detoxified biologically with microorganisms that specifically metabolize the fermentation inhibitors while preserving the sugars for subsequent use by the fermentation host. The bacterium Cupriavidus basilensis HMF14 was isolated from enrichment cultures with HMF as the sole carbon source and was found to metabolize many of the toxic constituents of lignocellulosic hydrolysate including furfural, HMF, acetate, formate and a host of aromatic compounds. Remarkably, this microorganism does not grow on the most abundant sugars in lignocellulosic hydrolysates: glucose, xylose and arabinose. In addition, C. basilensis HMF14 can produce polyhydroxyalkanoates. Cultivation of C. basilensis HMF14 on wheat straw hydrolysate resulted in the complete removal of furfural, HMF, acetate and formate, leaving the sugar fraction intact. This unique substrate profile makes C. basilensis HMF14 extremely well suited for biological removal of inhibitors from lignocellulosic hydrolysates prior to their use as fermentation feedstock. [source] Genetic characterisation of traditional chestnut varieties in Italy using microsatellites (simple sequence repeats) markersANNALS OF APPLIED BIOLOGY, Issue 1 2010M.A. Martín European chestnut (Castanea sativa) is an important multipurpose tree that has been cultivated for wood and fruit in the Mediterranean basin since ancient times. Cultivation of traditional chestnut varieties has a long tradition in Italy, where cultivars have been selected over centuries as a function of the best nut traits. In this study, 94 grafted chestnuts corresponding to 26 representative cultivars from Italy were evaluated by seven simple sequence repeat (SSR) markers to establish whether they corresponded to varieties in the narrow sense. The results allowed 20 genotypes to be identified that corresponded to the same number of clones. In total, 52 alleles were identified, eight of which were exclusive. Cases of homonymies and synonymies were detected. Moreover, our results highlighted a considerable genetic uniformity among ,Marrone-type' cultivars and, on the contrary, a high genetic diversity among the evaluated cultivars demonstrating that this is a valuable germplasm and an important genetic resource to be preserved. [source] Cultivation of Paracentrotus lividus (Echinodermata: Echinoidea) on extruded feeds: digestive efficiency, somatic and gonadal growthAQUACULTURE NUTRITION, Issue 2 2001Spirlet This study assessed the use of extruded feeds, in the form of pellets, for the growing of echinoid Paracentrotus lividus within a closed-culture system. Two feed types, one with soya-bean protein, the other with both soya-bean and fish protein were compared with dried Lessonia sp. and fresh Laminaria sp. as food sources. Pellets present a very high conversion efficiency (about 80%) against about 50% for Laminaria and 35% for Lessonia. However, as pellets are less absorbed, somatic growth (SG) is statistically equivalent for the sea urchins fed with pellets and Laminaria between 2 and 2.2% g soma day,1. Sea urchins fed with pellets produced significantly more gonadal tissue in a shorter time, resulting in a gonadal index (GI) twice higher (6.5%) than Laminaria (3%) in the second month of the experiment. Dry Lessonia does not promote gonadal growth (GG). This study shows that extruded feeds are well assimilated by P. lividus and promote both SG and production of gonadal tissue. [source] Spongy Polyethersulfone Membrane for Hepatocyte Cultivation: Studies on Human Hepatoma C3A CellsARTIFICIAL ORGANS, Issue 9 2008Andrzej Kinasiewicz Abstract:, There are different types of membranes used for hepatocyte cultivation. In our studies, spongy polyethersulfone (PES) membranes were examined as a support for hepatic cell cultivation in vitro. The extended surface of the membranes allows to introduce a high cell number especially in three-dimensional gel structure. Scanning electron microscopy analysis indicated that C3A cells used in our experiments grew well on PES membranes forming microvilli characteristic for normal hepatocytes. Analysis of cell viability proved that spongy PES membrane is well tolerated by J774 macrophages and did not stimulate nitric oxide synthesis. Bile canalicular structures were observed in fluorescence microscopy after F-actin staining with tetramethyl rhodamine iso-thiocyanate (TRITC)-phalloidin. The C3A cells showed high affinity to the PES membranes and adhered to almost 90% during the initial 24 h of incubation. Albumin production increased during static culture from the value of 805.2 ± 284.4 (ng/24 h/initial 106 cells) during the first days, to 2017.6 ± 505.9 (ng/24 h/initial 106 cells) after 10 days of culture. In conclusion, the spongy PES membranes can be used as scaffold for hepatocyte cultivation, especially for the creation of three-dimensional environments. [source] Optimization of cultivation conditions in spin tubes for Chinese hamster ovary cells producing erythropoietin and the comparison of glycosylation patterns in different cultivation vesselsBIOTECHNOLOGY PROGRESS, Issue 3 2010Jure Strnad Abstract This article describes the optimization of cultivation factor settings, that is the shaking rate and working volume in 50 mL spin tubes for a Chinese hamster ovary cell line expressing recombinant human ,-erythropoietin, using a response D-optimal surface method. The main objectives of the research were, firstly, to determine a setting in which the product titer and product quality attributes in spin tubes are equivalent to those in 250 mL shake flasks in a seven day batch and, secondly, to find a setting in which the product titer is maximal. The model for product titer prediction as a function of shaking rate and working volume in the defined design space was successfully applied to the optimization of cultivation conditions in spin tubes for the tested cell line. Subsequently, validation experiments were carried out simultaneously in spin tubes, shake flasks and bench scale bioreactors to compare cell culture performance parameters such as growth, productivity and product quality attributes in the form of isoform profiles and glycan antennarity structures. The results of the experiments showed that similar cell culture performance and product quality could be achieved in spin tubes when compared to shake flasks. Additionally, bioreactor titers could be reproduced in spin tubes at high shaking rates and low working volumes, but with differing product quality. Cultivation at lower shaking rates in spin tubes and shake flasks produced a glycoprotein with a product quality slightly comparable to that from bioreactors, but with titers being only two thirds. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010 [source] Development of a Novel Membrane Aerated Hollow-Fiber MicrobioreactorBIOTECHNOLOGY PROGRESS, Issue 2 2008Louis Villain A new challenge in biotechnological processes is the development of flexible bioprocessing platforms, allowing strain selection, facilitating scale-up and integrating separation steps. Miniaturization of such a cultivation system allows parallel use and the saving of resources but makes the supply of oxygen to the cells difficult. In this work we present a membrane aerated hollow-fiber microbioreactor (HFMBR) which consists of an acrylic glass module equipped with two different types of membrane fibers. Fibers of polyethersulfone and polyvinyldifluoride were used for substrate and oxygen supply, respectively. Cultivation of E. coli as model organism and production of His-tagged GFP were carried out in the extracapillary space of the membrane aerated HFMBR and compared with cultivations in shaking flask which are commonly used for screening experiments. The measurement of the oxygen transfer capacity and the online monitoring of the dissolved oxygen during the cultivation were performed using a fiber optic oxygen sensor. Online measurement of the optical density was also integrated to the bioreactor. Due to efficient oxygen transfer, a better cell growth than in the shaking flask experiments was achieved, while no negative influence on the GFP productivity was observed in the membrane aerated bioreactor. Thus the feasibility of a future integrated downstreaming could also be demonstrated. [source] Fed-Batch Cultivation of Saccharomyces cerevisiae in a Hyperbaric BioreactorBIOTECHNOLOGY PROGRESS, Issue 2 2003I. Belo Fed-batch is the dominating mode of operation in high-cell-density cultures of Saccharomyces cerevisaein processes such as the production of bakerapos;s yeast and recombinant proteins, where the high oxygen demand of these cultures makes its supply an important and difficult task. The aim of this work was to study the use of hyperbaric air for oxygen mass transfer improvement on S. cerevisiaefed-batch cultivation. The effects of increased air pressure up to 1.5 MPa on cell behavior were investigated. The effects of oxygen and carbon dioxide were dissociated from the effects of total pressure by the use of pure oxygen and gas mixtures enriched with CO2. Fed-batch experiments were performed in a stirred tank reactor with a 600 mL stainless steel vessel. An exponential feeding profile at dilution rates up to 0.1 h,1 was used in order to ensure a subcritical flux of substrate and, consequently, to prevent ethanol formation due to glucose excess. The ethanol production observed at atmospheric pressure was reduced by the bioreactor pressurization up to 1.0 MPa. The maximum biomass yield, 0.5 g g,1 (cell mass produced per mass of glucose consumed) was attained whenever pressure was increased gradually through time. This demonstrates the adaptive behavior of the cells to the hyperbaric conditions. This work proved that hyperbaric air up to 1.0 MPa (0.2 MPa of oxygen partial pressure) could be applied to S. cerevisiaecultivation under low glucose flux. Above that critical oxygen partial pressure value, i.e., for oxygen pressures of 0.32 and 0.5 MPa, a drastic cell growth inhibition and viability loss were observed. The increase of carbon dioxide partial pressure in the gas mixture up to 48 kPa slightly decreased the overall cell mass yield but had negligible effects on cell viability. [source] Fluid Mechanics, Cell Distribution, and Environment in Cell Cube BioreactorsBIOTECHNOLOGY PROGRESS, Issue 1 2003John G. Auni Cultivation of MRC-5 cells and attenuated hepatitis A virus (HAV) for the production of VAQTA, an inactivated HAV vaccine ( 1), is performed in the Cell Cube reactor, a laminar flow fixed-bed bioreactor with an unusual diamond-shaped, diverging-converging flow geometry. These disposable bioreactors have found some popularity for the production of cells and gene therapy vectors at intermediate scales of operation ( 2, 3). Early testing of the Cell Cube revealed that the fluid mechanical environment played a significant role in nonuniform cell distribution patterns generated during the cell growth phase. Specifically, the reactor geometry and manufacturing artifacts, in combination with certain inoculum practices and circulation flow rates, can create cell growth behavior that is not simply explained. Via experimentation and computational fluid dynamics simulations we can account for practically all of the observed cell growth behavior, which appears to be due to a complex mixture of flow distribution, particle deposition under gravity, fluid shear, and possibly nutritional microenvironment. [source] | ||||||||||||||||||||||||||||||||||||||||||||||