Cunninghamella Elegans (cunninghamella + elegan)

Distribution by Scientific Domains

Selected Abstracts

Purification and characterization of a glutathione S -transferase from the fungus Cunninghamella elegans

Chang-Jun Cha
Abstract Cunninghamella elegans grown on Sabouraud dextrose broth had glutathione S -transferase (GST) activity. The enzyme was purified 172-fold from the cytosolic fraction (120,000g) of the extract from a culture of C. elegans, using Q-Sepharose ion exchange chromatography and glutathione affinity chromatography. The GST showed activity against 1-chloro-2,4-dinitrobenzene, 1,2-dichloro-4-nitrobenzene, 4-nitrobenzyl chloride, and ethacrynic acid. Sodium dodecyl sulfate,polyacrylamide gel electrophoresis gel filtration chromatography revealed that the native enzyme was homodimeric with a subunit of Mr 27,000. Comparison by Western blot analysis implied that this fungal GST had no relationship with mammalian ,-, ,-, and ,-class GSTs, although it showed a small degree of cross-reactivity with a ,-class GST. The N-terminal amino acid sequence of the purified enzyme showed no significant homology with other known GSTs. [source]

Microbial Transformation of Mestranol by Cunninghamella elegans.

CHEMINFORM, Issue 3 2006
Muhammad Iqbal Choudhary
Abstract For Abstract see ChemInform Abstract in Full Text. [source]

Microbial Transformations of Gelomulide G: A Member of the Rare Class of Diterpene Lactones

Iqbal Choudhary
Microbial transformation of gelomulide G (3,,6,- diacetoxy-8,,14,- epoxyabiet-13(15)-en-16,12-olide, 1) was carried out. Incubation of 1 with Aspergillus niger afforded two new metabolites, 3,,6,- diacetoxy-8,,14,- dihydroxyabiet-13(15)-en-16,12-olide (2) and 3,,6,- diacetoxy-14,- hydroxyabieta-8(9),13(15)-dien-16,12-olide (3). While Cunninghamella elegans afforded the 14-epimer of 2, i.e., 3,,6,- diacetoxy-8,,14,- dihydroxyabiet-13(15)-en-16,12-olide (4), along with 3, -acetoxy-6,- hydroxy-8,,14,- epoxyabiet-13(15)-en-16,12-olide (5). The structures of the transformed products 2,5 were deduced to be new on the basis of MS and NMR data. [source]