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Cu Exposure (cu + exposure)
Selected AbstractsEffect of Cu stress on the invertase activity and root growth in two populations of Rumex dentatus L. with different Cu toleranceENVIRONMENTAL TOXICOLOGY, Issue 4 2008Yu Huang Abstract There has been no study on key enzymes in sucrose cleavage in metallophyte plants so far, which may be crucial for the plants' root growth and heavy metal tolerance maintenance. Acid invertases are rate-limiting enzymes in sucrose metabolism. Here, we tested the hypothesis that the roots of copper-tolerant plants should manifest a higher activity of acid invertases than nontolerant plants both for supporting growth and for their maintaining tolerance under Cu stress. Two populations of Rumex dentatus L., one from an ancient waste heap at a Cu mine (Cu-tolerant population), and the other from a noncontaminated site (Cu nontolerant population), were used in the experiments. The seedlings of Rumex dentatus L. were exposed to 0, 10, and 40 ,M CuCl2 for 14 days. Cu exposure had a stronger inhibition on root growth and thus resulted in a lower root/shoot ratio in the plants of nontolerant population compared with the Cu-tolerant population. Cu exposure showed a stronger inhibition of acid invertase activity of Cu nontolerant plants than Cu tolerant plants, whereas neutral/alkaline invertase was insensitive to Cu. A positive correlation between the activity of acid invertases and the root growth and root/shoot ratio was observed. The results suggested that the higher activities in acid invertases of Cu-tolerant population might at least partly associate with the plants' Cu tolerance, and their higher activities in acid invertases in turn played an role in maintenance of the Cu tolerance by supplying carbon and energy for tolerance mechanisms. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2008. [source] Chronic copper toxicity in the estuarine copepod Acartia tonsa at different salinitiesENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 10 2010Mariana M. Lauer Abstract Chronic Cu toxicity was evaluated in the euryhaline copepod Acartia tonsa. Male and female copepods were exposed (6 d) separately to different combinations of Cu concentration and water salinity (5, 15, and 30 ppt) using different routes of exposure (waterborne, waterborne plus dietborne, and dietborne). After exposure, groups of one male and three female copepods were allowed to reproduce for 24,h. In control copepods, egg production augmented with increasing water salinity. However, egg hatching rate did not change. Copper exposure reduced egg production and hatching rate in all water salinities tested, but the reproductive response was dependent on the route of Cu exposure. Median effective concentration (EC50) values for egg production after waterborne exposure were 9.9, 36.8, and 48.8,µg/L dissolved Cu at water salinities of 5, 15, and 30 ppt, respectively. For waterborne plus dietborne exposure, they were significantly higher (40.1, 63.7, and 109.9,µg /L, respectively). After dietborne exposure, approximately 40% decrease in egg production was observed, independently of Cu concentration and water salinity tested. At water salinities of 5 and 30 ppt, egg hatching rate reduced after waterborne exposure, together or not with the dietborne exposure. At water salinity of 15 ppt, Cu toxicity was only observed after dietborne exposure. Data indicate that egg production is a more reliable reproductive endpoint to measure chronic Cu toxicity in copepods than egg hatching rate in a wide range of water salinities. They also suggest that both water salinity and route of Cu exposure should be taken into account in the development of a chronic biotic ligand model version for estuarine and marine environments. Environ. Toxicol. Chem. 2010;29:2297,2303. © 2010 SETAC [source] Acute copper toxicity in the euryhaline copepod Acartia tonsa: implications for the development of an estuarine and marine biotic ligand modelENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 8 2010Grasiela Lopes Lećes Pinho Abstract Copepods (Acartia tonsa) were exposed (48 h) to waterborne, diet-borne (non-Cu-equilibrated and Cu-equilibrated food), and waterborne plus diet-borne Cu in either the absence or the presence of food (diatom Thalassiosira weissflogii). Toxicity tests were run in different salinities (5, 15, and 30 ppt) together with measurements of physicochemical parameters and total and dissolved Cu concentrations in the experimental media. Results show that most of the toxic Cu fraction was in the dissolved phase. In general, Cu toxicity was higher in low (5 ppt) than in high salinity (30 ppt), regardless of the pathway of Cu exposure tested. In the absence of food, data clearly indicate that differences in waterborne Cu toxicity can be explained by changes in water chemistry. However, addition of food (either non-Cu-equilibrated or Cu-equilibrated) to the experimental media protected against acute Cu toxicity in salinities 5 and 15 ppt, suggesting that A. tonsa requires extra energy to cope with the stressful condition imposed by Cu exposure associated with the ionoregulatory requirements in low salinities. For diet-borne exposure, a very high Cu concentration was necessary to precontaminate the diatoms to a level resulting in copepod mortality. Therefore, availability of food exerted a more important positive impact in protecting against acute Cu toxicity than its potential negative impact via contamination resulting in toxicity. Findings indicate the need for incorporation of both salinity and food in a future biotic ligand model (BLM) version for Cu in estuarine and marine waters. In this context, the euryhaline copepod A. tonsa would be a suitable model species with which to perform experiments to validate and calibrate any future saltwater BLM. Environ. Toxicol. Chem. 2010; 29:1834,1840. © 2010 SETAC [source] Development of photosynthetic biofilms affected by dissolved and sorbed copper in a eutrophic riverENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 9 2002Christiane Barranguet Abstract Photosynthetic biofilms are capable of immobilizing important concentrations of metals, therefore reducing bioavailability to organisms. But also metal pollution is believed to produce changes in the microalgal species composition of biofilms. We investigated the changes undergone by natural photosynthetic biofilms from the River Meuse, The Netherlands, under chronic copper (Cu) exposure. The suspended particles in the river water had only a minor effect on reduction of sorption and toxicity of Cu to algae. Biofilms accumulated Cu proportionally to the added concentration, also at the highest concentration used (9 ,M Cu). The physiognomy of the biofilms was affected through the growth of the chain-forming diatom Melosira varians, changing from long filaments to short tufts, although species composition was not affected by the Cu exposure. The Cu decreased phosphate uptake and algal biomass measured as chl a, which degraded exponentially in time. Photosynthetic activity was always less sensitive than algal biomass; the photon yield decreased linearly in time. The protective and insulating role of the biofilm, supported by ongoing autotrophic activity, was indicated as essential in resisting metal toxicity. We discuss the hypothesis that the toxic effects of Cu progress almost independently of the species composition, counteracting ongoing growth, and conclude that autotrophic biofilms act as vertical heterogeneous units. Effective feedback mechanisms and density dependence explain several discrepancies observed earlier. [source] Growth and Hematological Changes of Rockfish, Sebastes schlegeli (Hilgendorf) Exposed to Dietary Cu and CdJOURNAL OF THE WORLD AQUACULTURE SOCIETY, Issue 2 2005Ju-Chan Kang Cd and Cu toxicological effect on growth and hematological parameters was investigated in juvenile rockfish Sebasres schlegeli after sub-chronic dietary Cd (0, 0.5, 5, 25, and 125 mg/kg) and Cu exposure (0, 50, 125, 250, and 500 mg/kg) for 60 d. In the experiment of dietary Cd exposure, weight and length SGR of the rockfish were significantly different from control, and a significant inverse relationship was observed between weight gain and the exposure concentration of dietary Cd at 25, 125 mg/kg (P > 0.05). Hematwrit and hemoglobin decreased significantly and were dose dependently in all Cd exposure. Glucose in serum was also increased significantly (P < 0.05). The concentration of total protein in serum was significantly lower than control at 5, 25, and 125 mgkg. No differences were observed in serum calcium concentration. Magnesium concentration in serum was increased signillcantly with dietary Cd concentration. In the experiment of dietary Cu exposure, Cu was inhibited weight gain and growth rate. No differences were observed in hematocrit, hemoglobin and RBCs compared to control. Contents of total protein, glucose, and Ca in serum remained stable. Mg concentration in serum was increased significantly at 500 mg/kg. 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