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Adhesion Peptides (adhesion + peptide)

Distribution by Scientific Domains

Life Sciences	75%

Selected Abstracts

Dendrimer-grafted cell adhesion peptide,modified PDMS

BIOTECHNOLOGY PROGRESS, Issue 4 2008
A. S. Mikhail
Abstract Surface concentration of cell adhesion peptides is thought to play a role in the interactions between biomaterials and cells. The high density of functional groups at the periphery of dendrimers has been exploited in various applications, but their full potential for generating surfaces with high functional group concentrations has not yet been realized. Poly(dimethylsiloxane) elastomers were surface modified with both polyethylene oxide (PEO) and generation 3 diaminobutane dendrimers. PEO and the dendrimers were subsequently used as linker molecules for surface grafting of cell adhesion peptides. ATR-FTIR, X-ray photoelectron spectroscopy, and water contact angle results confirmed the successful attachment of the polymer linkers and peptides. Peptide grafting density was quantified by means of 125I radiolabeling. Maximum surface peptide grafting density on dendrimer-modified surfaces was twofold greater than the maximum peptide grafting density achieved via the PEO linker. However, vascular endothelial cell adhesion was significantly greater on surfaces modified with the PEO linker, presumably due to the highly flexible PEO spacer making the peptide more accessible for binding with the cell surface receptors. These results suggest that, although peptide surface density may be important, optimizing surface density may not be sufficient for improving biological interactions. [source]

Cell adhesion molecules for targeted drug delivery

JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 9 2006
Alison L. Dunehoo
Abstract Rapid advancement of the understanding of the structure and function of cell adhesion molecules (i.e., integrins, cadherins) has impacted the design and development of drugs (i.e., peptide, proteins) with the potential to treat cancer and heart and autoimmune diseases. For example, RGD peptides/peptidomimetics have been marketed as anti-thrombic agents and are being investigated for inhibiting tumor angiogenesis. Other cell adhesion peptides derived from ICAM-1 and LFA-1 sequences were found to block T-cell adhesion to vascular endothelial cells and epithelial cells; these peptides are being investigated for treating autoimmune diseases. Recent findings suggest that cell adhesion receptors such as integrins can internalize their peptide ligands into the intracellular space. Thus, many cell adhesion peptides (i.e., RGD peptide) were used to target drugs, particles, and diagnostic agents to a specific cell that has increased expression of cell adhesion receptors. This review is focused on the utilization of cell adhesion peptides and receptors in specific targeted drug delivery, diagnostics, and tissue engineering. In the future, more information on the mechanism of internalization and intracellular trafficking of cell adhesion molecules will be exploited for delivering drug molecules to a specific type of cell or for diagnosis of cancer and heart and autoimmune diseases. © 2006 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 95: 1856,1872, 2006 [source]

Selection and mass spectrometry characterization of peptides targeting semiconductor surfaces

BIOTECHNOLOGY & BIOENGINEERING, Issue 6 2009
Elias Estephan
Abstract We report on elaboration of 12-mer peptides that reveal specific recognition for the following semiconductor (SC) surfaces: GaAs(100), InAs(100), GaN(0001), ZnSe(100), ZnTe(100), GaAs(111)A, GaSb(100), CdSe(100). A M13 bacteriophage library was used to screen 109 different 12-mer peptides against these substrates to finally isolate, in maximum six amplification cycles, peptides that bind to the target surfaces. The specific peptides for the InAs and ZnSe surfaces were obtained. Contrary, for the other SC surfaces several peptides with high affinities have been isolated. Aiming for a better specificity, when the phage display has been conducted through six cycles, the screening procedure got dominated by a phage present in the M13 bacteriophage library and the SVSVGMKPSPRP peptide has been selected for different SCs. The high amplification potential of this phage has been observed previously with different targets. Thus, precaution should be undertaken in defining adhesion peptides with the phage display technique and real affinity of the obtained biolinkers should be studied with other methods. We employed mass spectrometry (MALDI-TOF/TOF) to demonstrate the preferential attachment (or not) of the SVSVGMKPSPRP peptide to the different SC surfaces. This allows us to define a realistic selection of the expressed peptides presenting affinity for the studied eight SC surfaces. We demonstrate that with increasing the dielectric constants of the employed solvents, adhesion of the SVSVGMKPSPRP peptide onto GaN(0001) is hindered. Biotechnol. Bioeng. 2009; 104: 1121,1131. © 2009 Wiley Periodicals, Inc. [source]