Corneal Stroma (corneal + stroma)

Distribution by Scientific Domains


Selected Abstracts


Keratocyte repopulation in UVB-exposed thioltransferase knockout mice

ACTA OPHTHALMOLOGICA, Issue 2007
A PODSKOCHY
Purpose: Thioltransferase is involved in cell protein homeostasis and DNA synthesis. It inhibits apoptosis and stimulates cell proliferation. Keratocyte repopulation after ultraviolet B (UVB) damage was studied in corneas of thioltransferase (-/-) mice. Methods: Six wild type mice and six thioltransferase (-/-) mice corneas were exposed at 300 nm UV-radiation at a dose producing damage in the corneal stroma (8 kJ/m2). Animals were killed 3 and 7 days after exposure. Corneas were processed for light microscopy. Results: All corneas of wild type mice and thioltransferase (-/-) mice showed extensive damage 3 days after UVB exposure. Keratocytes disappeared throughout the entire thickness of the UVB-damaged central stroma. Corneal thickness was nearly doubled compared with non-treated control corneas. However, 7 days after UVB exposure corneas of wild type mice were almost completely repopulated by keratocytes, only superficial ¼ of the stroma was still free of keratocytes. Corneal thickness was almost normal. Corneal stroma in the thioltransferase (-/-) mice 7 days after UV exposure was still not repopulated by keratocytes and the corneas were still very thick. Conclusions: The keratocyte repopulation in thioltransferase (-/-) mice is delayed. Thioltransferase seems to play an important role in the corneal wound healing and keratocyte repopulation after UVB induced damage. [source]


Development of the corneal stroma, and the collagen,proteoglycan associations that help define its structure and function

DEVELOPMENTAL DYNAMICS, Issue 10 2008
Andrew J. Quantock
Abstract The cornea of the eye is a unique, transparent connective tissue. It is comprised predominantly of collagen fibrils, remarkably uniform in diameter and regularly spaced, organized into an intricate lamellar array. Its establishment involves a precisely controlled sequence of developmental events in which the embryonic cornea undergoes major structural transformations that ultimately determine tissue form and function. In this article, we will review corneal developmental dynamics from a structural perspective, consider the roles and interrelationships of collagens and proteoglycans, and comment on contemporary concepts and current challenges pertinent to developmental processes that result in an optically clear, mature cornea. Developmental Dynamics 237:2607,2621, 2008. © 2008 Wiley-Liss, Inc. [source]


Human primary corneal fibroblasts synthesize and deposit proteoglycans in long-term 3-D cultures

DEVELOPMENTAL DYNAMICS, Issue 10 2008
R. Ren
Abstract Our goal was to develop a 3-D multi-cellular construct using primary human corneal fibroblasts cultured on a disorganized collagen substrate in a scaffold-free environment and to use it to determine the regulation of proteoglycans over an extended period of time (11 weeks). Electron micrographs revealed multi-layered constructs with cells present in between alternating parallel and perpendicular arrays of fibrils. Type I collagen increased 2,4-fold. Stromal proteoglycans including lumican, syndecan4, decorin, biglycan, mimecan, and perlecan were expressed. The presence of glycosaminoglycan chains was demonstrated for a subset of the core proteins (lumican, biglycan, and decorin) using lyase digestion. Cuprolinic blue,stained cultures showed that sulfated proteoglycans were present throughout the construct and most prominent in its mid-region. The size of the Cuprolinic-positive filaments resembled those previously reported in a human corneal stroma. Under the current culture conditions, the cells mimic a development or nonfibrotic repair phenotype. Developmental Dynamics 237:2705,2715, 2008. © 2008 Wiley-Liss, Inc. [source]


Drug-induced corneal hydration changes monitored in vivo by non-invasive confocal Raman spectroscopy

JOURNAL OF RAMAN SPECTROSCOPY, Issue 9 2001
Roel J. Erckens
It is well established that the state of corneal hydration plays a crucial role in maintaining optimal vision. Therefore, any knowledge that can be obtained non-invasively about the status of corneal hydration could be of significant clinical value. A novel confocal Raman spectroscopic technique was used to monitor non-invasively drug-induced hydration changes in the rabbit cornea. The spectroscopic technique enables one to monitor the changes in water content of the cornea while the confocal probing reduces interference of signals from adjacent tissues and allows for measurement of corneal hydration at various depths. The corneal hydration is altered by applying a dehydrating agent (Muro 128®) topically on the cornea. To determine the corneal hydration status, the OH/CH ratio between the Raman intensity of the water OH mode at 3390 cm,1 and the protein CH stretching mode at 2945 cm,1 is calculated. In the middle of the corneal stroma after 10 min, Muro 128® -treated corneas show an average decrease of about 30% in the OH/CH ratio (1.27 ± 0.13) compared with the untreated corneas (1.76 ± 0.09). In this in vivo model it is possible to monitor the hydration status of the living cornea using the Raman spectroscopic technique. Copyright © 2001 John Wiley & Sons, Ltd. [source]


Infectious keratitis related to orthokeratology

OPHTHALMIC AND PHYSIOLOGICAL OPTICS, Issue 2 2006
Xuguang Sun
Abstract Purpose:, To report 28 cases of infectious keratitis related to orthokeratology lens overnight wear in China. Methods:, From March 2000 to August 2001, 28 cases of infectious keratitis related to overnight orthokeratology lens wear were diagnosed in Beijing Institute of Ophthalmology. These were retrospectively reviewed with regard to the pathogens isolated, duration of wear, the time since onset of symptoms, and age. Cultures of corneal scrapes for bacteria, fungus and Acanthamoeba were performed in all of the 28 cases. Results:, All cases were students, including 10 males and 18 females, average age was 16 years (range 10,21 years). The duration of orthokeratology overnight wearing was from 2 weeks to 2 years. Uncorrected visual acuity (UCVA) on initial examination in our institute was from 20/200 to light perception. Of 28 isolates, 24 were culture positive (including 11 bacteria, 11 Acanthamoeba and two fungi), and four were culture negative. In two of the four culture negative cases, Acanthamoeba cysts were detected in the corneal stroma with the confocal microscope. Acanthamoeba and Pseudomonas aeruginosa accounted for 75% (21 of 28) of the cases of infectious keratitis. Conclusion:, Infectious keratitis is a severe complication associated with overnight orthokeratology lens wear. Ophthalmologists should pay more attention to this complication in practice. [source]


Filaria/Wolbachia activation of dendritic cells and development of Th1-associated responses is dependent on Toll-like receptor 2 in a mouse model of ocular onchocerciasis (river blindness)

PARASITE IMMUNOLOGY, Issue 9 2007
K. DAEHNEL
SUMMARY Toll-like receptors (TLRs) regulate dendritic cell function and activate signals that mediate the nature of the adaptive immune response. The current study examined the role of TLRs in dendritic cell activation and in regulating T cell and antibody responses to antigens from the filarial parasites Onchocerca volvulus and Brugia malayi, which cause river blindness and lymphatic filariasis, respectively. Bone-marrow-derived CD11c+ cells from C57BL/6 and TLR4,/, mice produced high levels of IL-6 and RANTES, and showed elevated surface CD40 expression, whereas CD11c+ cells from myeloid differentiation factor 88,/, (MyD88,/,), TLR2,/, and TLR2/4,/, mice were not activated. Similarly, IFN-, production by splenocytes from immunized TLR2,/, mice was significantly impaired compared with splenocytes from C57BL/6 and TLR4,/, mice. In contrast, there was no difference among these strains in Th2-associated responses including IL-5 production by splenocytes from immunized animals, serum IgE and IgG1, or eosinophil infiltration into the corneal stroma. Neutrophil recruitment to the cornea and CXC chemokine production was inhibited in immunized TLR2,/, mice compared with C57BL/6 and TLR4,/, mice. Taken together, these findings demonstrate an essential role for TLR2 in filaria-induced dendritic cell activation, IFN-, production and neutrophil migration to the cornea, but does not affect filaria-induced Th2-associated responses. [source]


3426: Straylight and corneal edema

ACTA OPHTHALMOLOGICA, Issue 2010
IG PENTARI
Purpose It is known that corneal edema is associated to increased light scatter. It was the purpose of this study to calculate the intensity and angular distribution of scattered light in a series of corneal samples at different hydration, using a sensitive optical technique. Methods Sixteen fresh porcine eyes were obtained from a local abattoir. To isolate the role of corneal stroma the the epithelium was carefully removed with a mechanical brush. The central 8 mm of each cornea was harvested using a Barron's PKP trephine. To establish corneal hydration, corneal buttons were immersed in Dextran (300kDa) solutions, with concentrations ranging from 5 to 20% w/w, for at least 3 hours. The intensity and angular distribution of scattered light was measured for all corneas by means of a purposely-developed camera lens that incorporated excised corneas between its glass elements. This lens was used with a CCD camera to record images projected on a computer screen. Before scatter measurements the thichness of each corneal sample was measured by means of a mechanical pachymeter (Mitutoyo IDC 112T, Japan). Results The mean scatter coefficients for the corneas at normal hydration levels was 0.22 (SD=0.059). This value was effectively double (0.46 ; SD= 0.048) at a moderate increase of stromal thickness by 17% and reached a value of 0.56 (SD=0.079) at a relative increase of corneal thickness of 62%. The angular distribution did not significantly deppend on hydration. Conclusion Scattered light intensity increases with corneal edema. Even small changes in corneal hydration affect significantly the narrow angle light scattering properties of corneal stroma. [source]


2142: Ulrastructural features of keratoconus cornea after cross-linking by riboflavin/UVA

ACTA OPHTHALMOLOGICA, Issue 2010
S AKHTAR
Purpose In the present studies we assess the effects of collagen cross-linking on ultrastructure organisation of the corneal stroma of keratoconus human corneas. Methods One normal, one keratoconus (KC) and three cross-linked keratoconus corneas were analysed. One was treated with standard cross linking (SXL) and two with trans-epithelial collagen cross linking (TEXL). Penetrating keratoplasty was performed three months after treatment. All samples were fixed in 2.5% glutaraldehyde containing cuprolinic blue in sodium acetate buffer and processed for electron microscopy. Results The structure of SXL corneas was very similar to normal corneas in their hemidesmosomes, basement membrane (BM), Bowman's layer (BW) and stromal lamellae that were not undulated. The architecture of TEXL corneas presented some differences. The BM was thick with degenerated hemidesmosomes. Bowman's layer was disorganised at some places and replaced by thin filaments forming pannus. There were thin undulating lamellae in anterior, middle and posterior stroma. The keratocytes were embedded between undulating lamellae. Large amounts of abnormal PGs were attached around collagen fibrils. The parallel running lamellae were very thin. In some parts of the anterior stroma collagen fibrils were oriented (running) in random directions instead of running parallel. There were some parts of the stroma which showed a normal appearance. Conclusion The present studies demonstrate that corneal cross-linking leads to modifications in keratocytes and in the organisation of collagen fibril. The morphological changes might be correlated to the process of increase in biomechanical stability although there are differences between stromal structures treated by standard and trans [source]


4135: Matrix metalloproteinase 14 overexpression reduces corneal scarring

ACTA OPHTHALMOLOGICA, Issue 2010
S GALIACY
Purpose Corneal wound healing is an everyday preoccupation for ophthalmologists.Corneal transparency depends on the scarring quality after a traumatic corneal wound, but also after refractive corneal surgery. Cicatrisation and fibrosis formation involve epithelial/fibroblast interactions via paracrin signals inducing extracellular matrix (ECM) remodelling. The major event is fibroblast activation and differentiation into myofibroblasts. These cells have a key role in the fibrotic response. They acquire contractile properties, and synthetise a new ECM, mainly composed of type III collagen. This scar tissue is less organised than the regular stroma, thus explaining corneal opacity. ECM remodelling is a critical step which aims to digest the excess of ECM by proteolysis of type III collagen. MMP14 is a membrane-bound fibrillar collagenase from the Matrix Metalloprotease family. We hypothesised that its overexpression in the corneal stroma during wound repair will increase ECM remodelling and thus prevent collagen deposition in the scar tissue. Methods We developed an adeno-associated virus-based vector expressing murine MMP14 under the control of the CMV promoter. We evaluated MMP14 overexpression after viral transfection in a murine model of corneal wound healing. We characterised several parameters: clinical observation, histology, and wound healing markers. Results Our preliminary results showed a decreased in oedema and corneal scar formation, associated with a decreased expression of alpha smooth actin and type III collagen. Conclusion These results represent proof of concept that gene transfer of MMP14 can reduce scar formation, which could have therapeutic applications after corneal trauma. [source]


Crystal deposits on the lens capsules in Bietti crystalline corneoretinal dystrophy associated with a mutation in the CYP4V2 gene

ACTA OPHTHALMOLOGICA, Issue 5 2010
Yumiko Yokoi
Abstract. Purpose:, We report a patient (Case 1) with Bietti crystalline corneoretinal dystrophy (BCD) associated with previously unknown findings of crystal-like deposits on the anterior and posterior lens capsules. This patient is one of four (Cases 1,4) in whom we have found BCD associated with the same mutation in the CYP4V2 gene. Methods:, We present a case report with molecular diagnosis. A 45-year-old man (Case 1) was referred to our clinic with complaints of gradual progression of visual disturbances and night blindness. His visual acuity was limited to hand movement bilaterally. Slit-lamp biomicroscopy disclosed glistening, crystal-like deposits on the anterior and posterior lens capsules, as well as on the corneal stroma near the corneoscleral limbus. No such deposit was found in the lens stroma. Fundus examination disclosed profound chorioretinal atrophy with scarce crystal deposits. Full-field electroretinography showed extinguished responses of isolated rods, isolated cones, and mixed rods and cones. Results:, Molecular genetic analysis revealed that the subject had a homozygous mutation in the CYP4V2 gene (IVS6,8delTCATACAGGTCATCGCG/insGC), which is most commonly found in Japanese patients with BCD. Three other cases (Cases 2,4) of BCD associated with the same mutation did not show such crystal-like deposits on the lens surface. Conclusions:, Although their exact origin remains unknown, crystal-like deposits may appear on the lens capsule of patients with BCD associated with a mutation in the CYP4V2 gene. [source]


Donor corneal stroma and host,donor interface vascularization after Descemet's membrane stripping with automated endothelial keratoplasty

ACTA OPHTHALMOLOGICA, Issue 2 2010
Katayoon B. Ebrahimi
First page of article [source]


Role of epidermal growth factor receptor (EGFR) in corneal remodelling in diabetes

ACTA OPHTHALMOLOGICA, Issue 8 2009
Saeed Akhtar
Abstract. Purpose:, This study examined the role of epidermal growth factor receptor (EGFR) signalling on the organization and remodelling of collagen fibrils (CFs) and proteoglycans (PGs) in the stroma of diabetic rat cornea. Methods:, Diabetes was induced in female Wistar rats (n = 5) by streptozotocin (STZ) injection (55 mg/kg). Treatment with a selective inhibitor of EGFR tyrosine kinase, AG1478, was started on the same day as the induction of diabetes and administered every other day for 4 weeks. Corneas were fixed in 4% paraformaldehyde at 4 ° to allow for analysis of CF diameters and in 2.5% glutaraldehyde in sodium acetate buffer containing cuprolinic blue to enable the study of PG distribution. AnalySIS soft imaging software was used to analyse CFs and PGs. Results:, Epithelial thickness, and median diameter and area fraction of CF in corneal stroma were decreased in diabetic rat cornea compared with normal cornea (p < 0.001), whereas the median PG area and area fractions were significantly increased (p < 0.001). Treatment with AG1478, although it had no action on normal cornea, prevented these diameter and area fraction changes in CFs and PGs. The cornea of AG1478-treated diabetic rats showed a slight increase in CF diameter and area fraction and a decreased number density. Conclusions:, These data show that the distribution of corneal stroma CFs and PGs was altered after 4 weeks of diabetes and that, furthermore, treatment with an EGFR signalling inhibitor normalized these abnormalities. The data suggest that EGFR plays an important role in the development of diabetes-induced corneal remodelling. [source]


Biosynthetic corneas , evaluation in humans

ACTA OPHTHALMOLOGICA, Issue 2009
P FAGERHOLM
Collagen-based biosynthetic corneas, designed to mimic the extracellular matrix of the corneal stroma have been developed and extensively evaluated in animal models over the last 7 years. Human recombinant collagen type III (RHC III) was crosslinked with water-soluble carbodiimides and fabricated into optically transparent corneal substitutes for transplantation. Following study approval of the Medical Product Agency, Sweden and the Human Ethics Committee, University of Linköping, Sweden, a Phase I study was initiated. 10 patients who were scheduled for corneal grafting were enrolled into the study. Nine had keratoconus and one had a deep scar following Pseudomonas keratitis. A central 6 mm diameter deep lamellar button was excised and was replaced by a 6.25 mm diameter 500 µm thick construct. Six overlying sutures were used to anchor the graft. Topical 0.1% dexametasone and chloramphenicol was used for the first 1 month postoperatively. The sutures were removed after 5-7 weeks. The patients were followed clinically and evaluated for UCVA, BSCVA and VA with contact lenses. Corneal touch sensitivity (Cochet-Bonnet) and tear production (Schirmer ) were tested. Photography, OCT (Visante), topography (Orbscan II) and in vivo confocal microscopy (Heidelberg) was documented. After 3 months all patients had stably epithelialized and implants were anchored by recipient keratocyte ingrowth. The mean BSCVA at 6 months (20/133) improved slightly at 12 months (20/90). The mean BCLCVA was 20/50 at 12 months and was notably better in younger patients (mean of 20/40 in the 5 youngest). One patient had BCLVA of 20/20 at 12 months. The mean central corneal thickness was stable between 3 and 12 months at about 400µm. The mean 5min Schirmer values were 20 ± 10mm in operated eyes and 17 ± 8 mm in fellow eyes. At 12 months the mean touch sensitivity was 25mm in operated eyes and 60mm in fellow eyes, which was the same as in penetrating grafts. In-vivo confocal microscopy revealed the ingrowth of corneal nerves at the subbasal epithelium. We have shown for the first time that bioengineered collagen-based corneal substitutes are fully compatible and promote regeneration of corneal cells. The 18 months follow-up results will be presented aswell. [source]


Distribution of amyloid and BIG-H3 in the cornea and limbus of a patient with lattice corneal dystrophy.

ACTA OPHTHALMOLOGICA, Issue 2009
Unique findings in donated eyes
Purpose The lattice corneal dystrophies (LCDs) are hereditary diseases involving the formation of opaque or refractile, amyloid-containing filaments in the corneal stroma. We report the distribution of amyloid and big-h3 protein in cornea and limbus in a patient suffering with LCD. Methods An 84 year-old patient with lattice corneal dystrophy died and donated her eyes for further study. The corneal and limbal tissue of the patient processed for light and electron microscopy. The primary polyclonal antibody big-h3 was located by secondary, goat anti-rabbit antibody conjugated with gold. Results In cornea amyloid deposits were observed below epithelium, and in the anterior and middle stroma. The epithelium was thin and invaginated by the amyloid deposits. In the limbus, large numbers of amyloid deposits were observed in sub-epithelial region, and in the mid and deep stroma. Subepithelial amyloid was also present in the substantia propria beneath the bulbar conjunctival epithelium. The amyloid deposits contained very thin amyloid fibrils and strongly stained with big-h3 antibody. There were also numerous long spacing collagen fibrils observed in the mid stroma, which also labelled with the antibody. Conclusion This is the first report of structural changes in the peripheral cornea and limbus in LCD. It is thought that mutated big-h3 protein diffuse into the stroma from the corneal epithelium to form amyloid deposits. The presence of amyloid and big-h3 at the limbus and in the adjacent bulbar conjunctiva and perilimbal cornea, suggests that Big-h3 is overproduced in these regions, which are normally free from clinically detectable disease. [source]


The distribution of neuroglobin in mouse eye

ACTA OPHTHALMOLOGICA, Issue 2009
Y YOU
Purpose To determine the distribution of neuroglobin (Ngb) in mouse eye. Ngb is predominantly expressed in the nervous system,and at particularly high levels in the retina. Ngb may serve as a reactive oxygen scavenger and may protect the tissue of eye from ischemia/hypoxia injuries. However,the distribution of Ngb in the eye is still controversial. Methods Two polyclonal antibodies against Ngb were used in this immunohistochemical study, both of which were raised in rabbits. One of these two antibodies was generated against the whole recombinant protein of mouse Ngb and the other was generated against amino acid positions 55-70 of mouse and human Ngb. The expression of Ngb was analyzed with light microscopy on tissue sections. Results These two antibodies showed comparable results. Ngb was expressed in the layers of the retina, including the ganglion cell layer, inner and outer nuclear layers, inner and outer plexiform layers, the inner segments of the photoreceptors and the retinal pigment epithelium. Ngb was also detected in other structures of the eye, including the epithelium and endothelium of cornea,the stroma of iris,the ciliary body, the lens epithelium, and the sclera. However, Ngb was not expressed in the corneal stroma, the lens capsule, the lamellar fibers of lens, the pigment epithelium of ciliary body or the pigment layer of iris. Conclusion Ngb was found widely distributed in mouse eye. This finding can be explained by the fact that most of the structures of the eye originated from neural crest/neural ectoderm. Future experiments will focus on the distribution of Ngb at the mRNA level (in situ hybridization),and the quantitative expression levels at baseline and after hypoxic/ischemic challenge. [source]


Keratocyte repopulation in UVB-exposed thioltransferase knockout mice

ACTA OPHTHALMOLOGICA, Issue 2007
A PODSKOCHY
Purpose: Thioltransferase is involved in cell protein homeostasis and DNA synthesis. It inhibits apoptosis and stimulates cell proliferation. Keratocyte repopulation after ultraviolet B (UVB) damage was studied in corneas of thioltransferase (-/-) mice. Methods: Six wild type mice and six thioltransferase (-/-) mice corneas were exposed at 300 nm UV-radiation at a dose producing damage in the corneal stroma (8 kJ/m2). Animals were killed 3 and 7 days after exposure. Corneas were processed for light microscopy. Results: All corneas of wild type mice and thioltransferase (-/-) mice showed extensive damage 3 days after UVB exposure. Keratocytes disappeared throughout the entire thickness of the UVB-damaged central stroma. Corneal thickness was nearly doubled compared with non-treated control corneas. However, 7 days after UVB exposure corneas of wild type mice were almost completely repopulated by keratocytes, only superficial ¼ of the stroma was still free of keratocytes. Corneal thickness was almost normal. Corneal stroma in the thioltransferase (-/-) mice 7 days after UV exposure was still not repopulated by keratocytes and the corneas were still very thick. Conclusions: The keratocyte repopulation in thioltransferase (-/-) mice is delayed. Thioltransferase seems to play an important role in the corneal wound healing and keratocyte repopulation after UVB induced damage. [source]


Emergency treatment of chemical and thermal eye burns

ACTA OPHTHALMOLOGICA, Issue 1 2002
Ralf Kuckelkorn
ABSTRACT. Chemical and thermal eye burns account for a small but significant fraction of ocular trauma. The speed at which initial irrigation of the eye begins, has the greatest influence on the prognosis and outcome of eye burns. Water is commonly recommended as an irrigation fluid. However, water is hypotonic to the corneal stroma. The osmolarity gradient causes an increased water influx into the cornea and the invasion of the corrosive substance into deeper corneal structures. We therefore recommend higher osmolarities for the initial rinsing to mobilize water and the dissolved corrosives out of the burnt tissue. Universal systems such as amphoteric solutions, which have an unspecific binding with bases and acids, provide a convenient solution for emergency neutralisation. Both conservative anti-inflammatory therapy and early surgical intervention are important to reduce the inflammatory response of the burnt tissue. In most severe eye burns, tenonplasty re-establishes the conjunctival surface and limbal vascularity and prevents anterior segment necrosis. [source]


Immunohistological study of infiltrated cells and cytokines in murine herpetic keratitis

ACTA OPHTHALMOLOGICA, Issue 5 2001
Tomoyuki Inoue
ABSTRACT. Purpose: To identify localization and kinetics of infiltrated cells and cytokines in murine herpetic keratitis. Methods: HSV-1 was inoculated onto the scarified BALB/c corneas. At given times post infection (PI), eyes were removed and studied immunohistochemically using monoclonal antibodies against several infiltrated cells and cytokines. Results: Neutrophils and NK cells infiltrated as early as 1 day PI reaching a maximum number at 2 day PI in initial stage. ,, TCR positive cells were observed in the corneal stroma from 1 day PI to 8 day PI. IL-2 and IFN-, were positive in the cell-infiltrated areas of the epithelial and stromal lesions, whereas IL-4 was negative throughout the experiment. Conclusion: Our results indicated that cytokine profile upon herpes infection on the cornea is Th1 dominant. Together with neutrophils in the early phase of infection, ,, positive T cells may play an additional role in protecting the cornea against incoming pathogens. [source]


Corneal injury by wild taro

CLINICAL & EXPERIMENTAL OPHTHALMOLOGY, Issue 9 2006
Emily WH Tang MRCS
Abstract We report a case of crystalline keratopathy caused by Alocasia macrorrhiza. The diagnosis was made based on the observation of needle-like crystals in the corneal stroma following injury to that eye. The condition resolved in 3 months with the disappearance of the crystals confirmed by follow-up confocal microscopy. [source]