Complex Event (complex + event)

Distribution by Scientific Domains


Selected Abstracts


Assessment of flooding in urbanized ungauged basins: a case study in the Upper Tiber area, Italy

HYDROLOGICAL PROCESSES, Issue 10 2005
T. Moramarco
Abstract The reliability of a procedure for investigation of flooding into an ungauged river reach close to an urban area is investigated. The approach is based on the application of a semi-distributed rainfall,runoff model for a gauged basin, including the flood-prone area, and that furnishes the inlet flow conditions for a two-dimensional hydraulic model, whose computational domain is the urban area. The flood event, which occurred in October 1998 in the Upper Tiber river basin and caused significant damage in the town of Pieve S. Stefano, was used to test the approach. The built-up area, often inundated, is included in the gauged basin of the Montedoglio dam (275 km2), for which the rainfall,runoff model was adapted and calibrated through three flood events without over-bank flow. With the selected set of parameters, the hydrological model was found reasonably accurate in simulating the discharge hydrograph of the three events, whereas the flood event of October 1998 was simulated poorly, with an error in peak discharge and time to peak of ,58% and 20%, respectively. This discrepancy was ascribed to the combined effect of the rainfall spatial variability and a partial obstruction of the bridge located in Pieve S. Stefano. In fact, taking account of the last hypothesis, the hydraulic model reproduced with a fair accuracy the observed flooded urban area. Moreover, incorporating into the hydrological model the flow resulting from a sudden cleaning of the obstruction, which was simulated by a ,shock-capturing' one-dimensional hydraulic model, the discharge hydrograph at the basin outlet was well represented if the rainfall was supposed to have occurred in the region near the main channel. This was simulated by reducing considerably the dynamic parameter, the lag time, of the instantaneous unit hydrograph for each homogeneous element into which the basin is divided. The error in peak discharge and time to peak decreased by a few percent. A sensitivity analysis of both the flooding volume involved in the shock wave and the lag time showed that this latter parameter requires a careful evaluation. Moreover, the analysis of the hydrograph peak prediction due to error in rainfall input showed that the error in peak discharge was lower than that of the same input error quantity. Therefore, the obtained results allowed us to support the hypothesis on the causes which triggered the complex event occurring in October 1998, and pointed out that the proposed procedure can be conveniently adopted for flood risk evaluation in ungauged river basins where a built-up area is located. The need for a more detailed analysis regarding the processes of runoff generation and flood routing is also highlighted. Copyright © 2005 John Wiley & Sons, Ltd. [source]


The regulation of HIV-1 transcription: Molecular targets for chemotherapeutic intervention

MEDICINAL RESEARCH REVIEWS, Issue 5 2006
Miguel Stevens
Abstract The regulation of transcription of the human immunodeficiency virus (HIV) is a complex event that requires the cooperative action of both viral and cellular components. In latently infected resting CD4+ T cells HIV-1 transcription seems to be repressed by deacetylation events mediated by histone deacetylases (HDACs). Upon reactivation of HIV-1 from latency, HDACs are displaced in response to the recruitment of histone acetyltransferases (HATs) by NF-,B or the viral transcriptional activator Tat and result in multiple acetylation events. Following chromatin remodeling of the viral promoter region, transcription is initiated and leads to the formation of the TAR element. The complex of Tat with p-TEFb then binds the loop structures of TAR RNA thereby positioning CDK9 to phosphorylate the cellular RNA polymerase II. The Tat-TAR-dependent phosphorylation of RNA polymerase II plays an important role in transcriptional elongation as well as in other post-transcriptional events. As such, targeting of Tat protein (and/or cellular cofactors) provide an interesting perspective for therapeutic intervention in the HIV replicative cycle and may afford lifetime control of the HIV infection. © 2006 Wiley Periodicals, Inc. Med Res Rev, 26, No. 5, 595,625, 2006 [source]


Homing of transplanted bone marrow cells in livers of Schistosoma mansoni -infected mice

APMIS, Issue 4 2010
NAGWA ELKHAFIF
Elkhafif N, Voss B, Hammam O, Yehia H, Mansy S, Akl M, Boehm S, Mahmoud S, El Bendary O, El Fandy G. Homing of transplanted bone marrow cells in livers of Schistosoma mansoni -infected mice. APMIS 2010; 118: 277,87. The efficiency of differentiation of bone marrow cells (BMCs) into hepatocytes in vivo and its importance in physiopathological processes is still debated. Murine schistosomiasis was used as a liver injury model and unfractionated male mice BMCs were transplanted through intrahepatic injection into non-irradiated Schistosoma mansoni -infected female mice on their 16th week post-infection. Two weeks after bone marrow transplantation, mice were sacrificed on a weekly basis until 10 weeks. Tracing of male donor-derived cells in female recipient mice livers was carried out by the detection of Y chromosome expression by fluorescent in situ hybridization (FISH) and also of chromodomain Y-linked (CDYL) protein by indirect immunofluorescence (IF). Their transformation into hepatocytes was studied by double labelling indirect IF using antibodies directed against CDYL and mouse albumin. Histopathological and electron microscopic examinations revealed the presence of small hepatocyte-like cells in the periportal tracts and in between the hepatocytes facing the sinusoids. Donor-derived cells showing Y chromosome by FISH and expressing CDYL protein by IF were recovered in the infected transplanted livers. The initial number of these cells increased with increased post-transplantation time. Cells were mainly localized in the periphery of schistosoma granuloma. Few donor-derived cells appeared within the hepatic parenchymal tissue and showed positivity for albumin secretion by double labelling with IF. We suggest that transplanted bone marrow stem cells can repopulate the Schistosoma -infected liver of immunocompetent mice. Their differentiation is a complex event controlled by many factors and needs to be further characterized extensively. The extent and type of liver injury and the number of transplanted cells are important variables in the process of stem cell engraftment and differentiation into functioning hepatic cells that still need to be defined. [source]


Repeated partial eyewitness questioning causes confidence inflation but not retrieval-induced forgetting

APPLIED COGNITIVE PSYCHOLOGY, Issue 1 2009
Geralda Odinot
During a crime investigation eyewitnesses are often interviewed more than once. Repeated post-event questioning offers an opportunity for retrieval practice. Practicing retrieval of a subset of memories may suppress access to related memories, a phenomenon known as retrieval-induced forgetting. In this short report we investigated the generalization of retrieval-induced forgetting to episodic eyewitness memory of a complex event. The results indicated that repeated retrieval improves future recall of practiced information, but does not induce forgetting of related information. Retrieval practice, however, did result in higher confidence ratings, both for correct and incorrect answers. The practical consequence of this study is that repeated questioning should be avoided if possible. Not because it may induce retrieval-induced forgetting, but because it may lead to confidence inflation. Copyright © 2008 John Wiley & Sons, Ltd. [source]


Genetic association of vitamin D receptor polymorphisms with primary biliary cirrhosis and autoimmune hepatitis

HEPATOLOGY, Issue 1 2002
Arndt Vogel
Autoimmune hepatitis (AIH) and primary biliary cirrhosis (PBC) are immune-mediated chronic inflammatory diseases of the liver of unknown etiology. Genetic factors appear to be involved in the pathogenesis of both diseases. 1,25-Dihydroxyvitamin D3 has been implicated as an immunomodulator, which acts through its own receptor (VDR). Polymorphisms of the VDR have been linked to a variety of autoimmune diseases. In this study VDR polymorphisms were analyzed in 123 patients with AIH, 74 patients with PBC, and 214 controls. VDR polymorphisms were assessed by BsmI, TaqI, ApaI, and Fok endonuclease digestion after specific polymerase chain reaction (PCR) amplification. We found a significant association between the BsmI polymorphisms in PBC patients in comparison with controls (,2 = 9.49, P = .009). Furthermore we detected a significant association of the Fok polymorphims in AIH patients in comparison to controls (,2 = 9.71, P = .008) indicating a genetic link of VDR polymorphisms to autoimmune liver diseases such as PBC and AIH in German patients. These findings contribute to the knowledge of the complex events determining immunologic tolerance in the liver. Further studies are needed to elucidate the mechanisms by which the vitamin D receptor contributes to the development of autoimmune diseases. [source]


Cell death mechanisms in neurodegeneration

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Issue 1 2001
K. A. Jellinger
Abstract Progressive cell loss in specific neuronal populations often associated with typical cytoskeletal protein aggregations is a pathological hallmark of neurodegenerative disorders, but the nature, time course and molecular causes of cell death and their relation to cytoskeletal pathologies are still unresolved. Apoptosis or alternative pathways of cell death have been discussed in Alzheimer's disease and other neurodegenerative disorders. Apoptotic DNA fragmentation in human brain as a sign of neuronal injury is found too frequent as to account for continous neuron loss in these slowly progressive processes. Morphological studies revealed extremely rare apoptotic neuronal death in Alzheimer's disease but yielded mixed results for Parkinson's disease and other neurodegenerative disorders. Based on recent data in human brain, as well as in animal and cell culture models, a picture is beginning to emerge suggesting that, in addition to apoptosis, other forms of programmed cell death may participate in neurodegeneration. Better understanding of the molecular players will further elucidate the mechanisms of cell death in these disorders and their relations to cytoskeletal abnormalities. Susceptible cell populations in a proapoptotic environment show increased vulnerability towards multiple noxious factors discussed in the pathogenesis of neurodegeneration. In conclusion, although many in vivo and in vitro data are in favor of apoptosis involvement in neurodegenerative processes, there is considerable evidence that very complex events may contribute to neuronal death with possible repair mechanisms, the elucidation of which may prove useful for future prevention and therapy of neurodegenerative disorders. [source]


How does acantholysis occur in pemphigus vulgaris: a critical review

JOURNAL OF CUTANEOUS PATHOLOGY, Issue 6 2006
Alessandro Lanza
Background:, Pemphigus vulgaris is a life-threatening autoimmune blistering disease targeting skin and mucous membranes, characterized by disruption of keratinocytes' adhesion termed acantholysis. Today multiple classes of targets are considered to play a role in the genesis of the acantholysis; of these, the classical pemphigus antigens, desmosomal cadherins (desmoglein 1 and 3) are the best characterized and considered as the most important. Additional antigens include the novel epithelial acetylcholine receptors (,9 and pemphaxin). Thus, acantholysis in pemphigus seems to result from a cooperative action of antibodies to different keratinocyte self-antigens, but the mechanisms by which epithelial cleft occurs are not yet clearly understood. In fact, the binding of the autoantibodies to these targets generates a plethora of biological effects due, on one hand, to their direct interference with adhesive function and, on the other, to more complex events involving intracellular pathways that modify proteases activity or calcium metabolism, leading to loss of cell,cell adhesion. [source]


Live imaging of fluorescent proteins in chordate embryos: From ascidians to mice

MICROSCOPY RESEARCH AND TECHNIQUE, Issue 3 2006
Yale J. Passamaneck
Abstract Although we have advanced in our understanding of the molecular mechanisms intrinsic to the morphogenesis of chordate embryos, the question of how individual developmental events are integrated to generate the final morphological form is still unresolved. Microscopic observation is a pivotal tool in developmental biology, both for determining the normal course of events and for contrasting this with the results of experimental and pathological perturbations. Since embryonic development takes place in three dimensions over time, to fully understand the events required to build an embryo we must investigate embryo morphogenesis in multiple dimensions in situ. Recent advances in the isolation of naturally fluorescent proteins, and the refinement of techniques for in vivo microscopy offer unprecedented opportunities to study the cellular and molecular events within living, intact embryos using optical imaging. These technologies allow direct visual access to complex events as they happen in their native environment, and thus provide greater insights into cell behaviors operating during embryonic development. Since most fluorescent protein probes and modes of data acquisition are common across species, we have chosen the mouse and the ascidian, two model organisms at opposite ends of the chordate clade, to review the use of some of the current genetically-encoded fluorescent proteins and their visualization in vivo in living embryos for the generation of high-resolution imaging data. Microsc. Res. Tech. 69:160,167, 2006. © 2006 Wiley-Liss, Inc. [source]