			Home About us Contact

Coconut Water (coconut + water)

Distribution by Scientific Domains

Life Sciences	37%

Selected Abstracts

Evaluation of Fertilizing Potential of Frozen-thawed dog Spermatozoa Diluted in ACP-106® using an In Vitro Sperm,Oocyte Interaction Assay

REPRODUCTION IN DOMESTIC ANIMALS, Issue 1 2007
RCS Cardoso
Contents The aim of present study was to evaluate frozen canine semen with ACP-106® (Powder Coconut Water) using an in vitro sperm,oocyte interaction assay (SOIA). Ten ejaculates from five stud dogs were diluted in ACP-106® containing 20% egg yolk, submitted to cooling in a thermal box for 40 min and in a refrigerator for 30 min. After this period, a second dilution was performed using ACP-106® containing 20% egg yolk and 12% glycerol. Samples were thawed at 38°C for 1 min. Post-thaw motility was evaluated by light microscopy and by using a computer aided semen analysis (CASA). Plasma membrane integrity and sperm morphology/acrosomal status were evaluated by fluorescent probes (C-FDA/PI) and Bengal Rose respectively. Moreover, frozen-thawed semen was analysed by a SOIA. Subjective post-thaw motility was 52.0 ± 14.8% and it was significant higher than the total motility estimated by CASA (23.0 ± 14.8%) because this system considered the egg yolk debris as immotile spermatozoa. Although normal sperm rate and acrosomal integrity evaluated by Bengal Rose stain was 89.6 ± 3.1% and 94.3 ± 3.1%, respectively, post-thaw percentage of intact plasma membrane was only 35.1 ± 14.3%. Regarding SOIA, the percentage of interacted oocytes (bound, penetrated and bound and/or penetrated) was 75.3%. Using regression analysis, it was found significant relations between some CASA patterns and data for SOIA. In conclusion, the freezing-thawing procedure using ACP-106® was efficient for maintain the in vitro fertility potential of dog spermatozoa. [source]

Coconut water as a potential resource for cellulose acetate membrane preparation

POLYMER INTERNATIONAL, Issue 3 2008
Cynthia Radiman
Abstract BACKGROUND: Cellulose acetate membranes are frequently used for pressure-driven membrane processes. The aim of this work was to prepare cellulose acetate membranes from nata-de-coco using coconut water as starting material. The use of this lignin-free material will certainly minimize the use of chemicals usually needed in the traditional pulps and substitute for the use of wood, which helps prevent global warming and preserves nature as well. RESULTS: Coconut water was fermented by Acetobacter xylinum for 6 days to produce nata-de-coco, which was then acetylated to produce cellulose diacetate with an acetyl content of 39.6%. Fourier transform infrared analysis showed characteristic peaks for the acetyl group at 1748 and 1236 cm,1. The resulting membranes made from the hydrolysis product showed a water flux of 210.5 L m,2 h,1 under an applied pressure of 2 kg cm,2 while the rejection coefficients of dextran T-500 and T-2000 solutions were 78 and 93.7%, respectively. CONCLUSION: Coconut water has a potential to be used in the fabrication of membranes by converting it to nata-de-coco and then to cellulose diacetate which gives an added value to its original nature. It is also highly competitive compared to the traditional pulps, by which acetylation decreases the degree of crystallinity of nata-de-coco resulting in higher membrane permeability. Copyright © 2007 Society of Chemical Industry [source]

Analyses of gibberellins in coconut (Cocos nucifera L.) water by partial filling-micellar electrokinetic chromatography-mass spectrometry with reversal of electroosmotic flow

ELECTROPHORESIS, Issue 10 2008
Liya Ge
Abstract In this paper, we present the results of simultaneous screening of eight gibberellins (GAs) in coconut (Cocos nucifera L.) water by MEKC directly coupled to ESI-MS detection. During the development of MEKC-MS, partial filling (PF) was used to prevent the micelles from reaching the mass spectrometer as this is detrimental to the MS signal, and a cationic surfactant, cetyltrimethylammonium hydroxide, was added to the electrolyte to reverse the EOF. On the basis of the resolution of the neighboring peaks, different parameters (i.e., the pH and concentration of buffer, surfactant concentrations, length of the injected micellar plug, organic modifier, and applied separation voltage) were optimized to achieve a satisfactory PF-MEKC separation of eight GA standards. Under optimum conditions, a baseline separation of GA standards, including GA1, GA3, GA5, GA6, GA7, GA9, GA12, and GA13, was accomplished within 25,min. Satisfactory results were obtained in terms of precision (RSD of migration time below 0.9%), sensitivity (LODs in the range of 0.8,1.9,,M) and linearity (R2 between 0.981 and 0.997). MS/MS with multiple reaction monitoring (MRM) detection was carried out to obtain sufficient selectivity. PF-MEKC-MS/MS allowed the direct identification and confirmation of the GAs presented in coconut water (CW) sample after SPE, while, the quantitative analysis of GAs was performed by PF-MEKC-MS approach. GA1 and GA3 were successfully detected and quantified in CW. It is anticipated that the current PF-MEKC-MS method can be applicable to analyze GAs in a wide range of biological samples. [source]

Continuous dense-phase CO2 processing of a coconut water beverage

INTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 4 2009
Sibel Damar
Summary Effects of dense-phase CO2 (DPCD) on microbial, physical, chemical and sensorial quality of coconut water (CW) beverage were evaluated. Pressure during DPCD treatment was not significant in microbial reduction whereas temperature and % CO2 levels were significant. DPCD-treated (34.5 MPa, 25 °C, 13% CO2, 6 min), heat-pasteurised (74 °C, 15 s) and untreated CW beverages were evaluated during 9 weeks of refrigerated storage (4 °C). Total aerobic bacteria of DPCD and heat-treated samples decreased whereas that of untreated samples increased to >105 CFU mL,1 after 9 weeks. DPCD increased titratable acidity but did not change pH (4.20) and °Brix (6.0). Likeability of DPCD-treated CW was similar to untreated. Heat-treated samples were less liked (, = 0.05) at the beginning of storage. Off flavour and taste-difference-from-control scores of heated samples were higher than DPCD during the first two weeks. DPCD extended shelf life of acidified, sweetened and carbonated CW over 9 weeks at 4 °C. [source]

Efficacy of sodium hypochlorite and peracetic acid in sanitizing green coconuts

LETTERS IN APPLIED MICROBIOLOGY, Issue 3 2009
E.H.M. Walter
Abstract Aims:, To evaluate the efficacy of sanitizing green coconuts (Cocos nucifera L.) through the treatment applied by juice industries using sodium hypochlorite and peracetic acid. Methods and Results:, The surface of the fruits was inoculated with a mixture of five Listeria monocytogenes strains. The treatments consisted in immersing the fruits for 2 min at room temperature in sodium hypochlorite solution containing 200 mg l,1 residual chlorine at pH 6·5, and 80 mg l,1 solution of peracetic acid or sterile water. Bacterial populations were quantified by culturing on trypticase soy agar supplemented with yeast extract and Oxford selective culture medium; however, recovery was higher on the nonselective medium. Immersion in water produced a reduction in the L. monocytogenes population of 1·7 log10 CFU per fruit, while immersion in sodium hypochlorite and peracetic acid solutions resulted in population reductions of 2·7 and 4·7 log10 CFU per fruit respectively. Conclusions:, The treatments studied are efficient to green coconuts. Significance and Impact of the Study:, Sanitation of green coconut is one of the most important control measures to prevent the contamination of coconut water. This article provides information that shows the adequacy of sanitizing treatments applied by the juice industries. [source]

Potential of bacterial indoleacetic acid to induce adventitious shoots in plant tissue culture

LETTERS IN APPLIED MICROBIOLOGY, Issue 2 2007
B. Ali
Abstract Aims:, The main aim of this study was to investigate the possible role of indoleacetic acid (IAA) from bacteria to induce in vitro adventitious shoots in internodal explants of Brassica oleracea L. Methods and Results:, Culture supernatant of Halomonas sp. RE1 and Halomonas sp. HT1 that contain 21 and 40 ,g ml,1 IAA, respectively, was used to supplement Murashige and Skoog (MS) medium. Two combinations that were supplemented with bacterial supernatant (BS) are MS + BS and MS + BS + 10%CW (coconut water) while basal MS medium was used as control. The amounts of BS used in this experiment were 50, 100, 150 and 200 ,l in 5 ml MS medium in each combination. In vitro -grown internodal explants of B. oleracea were inoculated on these media combinations and incubated in a growth chamber at 25 ± 1°C and exposed to 16-h cool fluorescent light. After 5,6 weeks of incubation adventitious shoot induction was observed in all treatments that were supplemented with BS as compared with the controls where very low response was observed. The frequency of shoot induction was high in media that were supplemented with 10%CW in the presence of bacterial auxin. Conclusions:, It was concluded that IAA of microbial origin has the potential to induce adventitious shoots in internodal explants. Significance and Impact of the Study:, IAA from bacteria can be effectively used in plant tissue culture; especially a combination of MS + BS + 10%CW is very cost-effective as compared with synthetic phytohormones for in vitro studies. [source]