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Ciocalteu Reagent (ciocalteu + reagent)

Distribution by Scientific Domains

Chemistry	100%

Selected Abstracts

ANTIOXIDANT ACTIVITY AND PHENOLIC ACID CONSTITUENTS OF CHESTNUT (CASTANIA SATIVA MILL.) HONEY AND PROPOLIS

JOURNAL OF FOOD BIOCHEMISTRY, Issue 4 2009
ALI OSMAN SARIKAYA
ABSTRACT This study describes the constituents of phenolic acids and antioxidant activities of chestnut (Castania sativa Mill.) honeys and propolis in Turkey. Antioxidant activity of the chestnut honeys and propolis were examined by three different methods, namely scavenging of free radical 2, 2-diphenyl-1-picrylhydrazyl, FRAP, and cupric reducing antioxidant power. Total phenolic contents were determined by using Folin,Ciocalteu reagent as GA equivalent. The phenolic constituents were also determined by HPLC. The antioxidant activities were compared with standard antioxidants such as catechin, BHT and Trolox. The antioxidant activities of all the samples were found high and related to the sample concentrations. The ethanolic propolis extracts showed the highest antioxidant activity. The major phenolic acids of the chestnut honeys and propolis identified by HPLC with PDA detection were coumaric acid, FA, cinnamic acid, CA and ChA. PRACTICAL APPLICATIONS In this study, some phenolic acid components and antioxidant capacity of chestnut (Castania sativa Mill.) honey and propolis were measured. The comparative findings from antioxidant activities and phenolic acid analyses of honey and propolis samples of chestnut origin provide important criteria for considering their nutritional and nutraceutical potentials. Comparison of our results with literature data also ranks the chestnut honey and propolis as better sources of antioxidants among those from other floral origins. [source]

Methodology Optimization for Quantification of Total Phenolics and Individual Phenolic Acids in Sweetpotato (Ipomoea batatas L.) Roots

JOURNAL OF FOOD SCIENCE, Issue 7 2007
M.S. Padda
ABSTRACT:, Phenolic acids are one of the several classes of naturally occurring antioxidant compounds found in sweetpotatoes. Simplified, robust, and rapid methodologies were optimized to quantify total and individual phenolic acids in sweetpotato roots. Total phenolic acid content was quantified spectrophotometrically using both Folin,Denis and Folin,Ciocalteu reagents. The Folin,Ciocalteu reagent gave an overestimation of total phenolic acids due to the absorbance of interfering compounds (that is, reducing sugars and ascorbic acid). Individual phenolic acids were quantified by high-performance liquid chromatography (HPLC) using the latest in column technology. Four reversed-phase C18 analytical columns with different properties (dimensions, particle size, particle shape, pore size, and carbon load) were compared. Three different mobile phases using isocratic conditions were also evaluated. A column (4.6 × 150 mm) packed with 5-,m spherical silica particles of pore size 110 Å combined with 14% carbon load provided the best and fast separation of individual phenolic acids (that is, chlorogenic acid, caffeic acid, and 3 isomers of dicaffeoylquinic acid) with a total analysis time of less than 7 min. Among the 3 mobile phases tested, a mobile phase consisting of 1% (v/v) formic acid aqueous solution: acetonitrile: 2-propanol, pH 2.5 (70:22:8, v/v/v) gave adequate separation. Among the solvents tested, aqueous mixtures (80:20, solvent:water) of methanol and ethanol provided higher phenolic acid extraction efficiency than the aqueous mixture of acetone. [source]

Phenolic compounds in the brown seaweed Ascophyllum nodosum: distribution and radical-scavenging activities

PHYTOCHEMICAL ANALYSIS, Issue 5 2010
Laetitia Audibert
Abstract Introduction , Phenolic compounds are metabolites exhibited at high levels in Phaeophyceae. Although several studies have been conducted on total phenol contents, no one to our knowledge has dealt with the contents of phenolic compounds and antioxidant activities on purified fractions. Objective , The purpose of this study was the extraction and purification of phenolic compounds from the brown seaweed Ascophylllum nodosum, to determine both their distribution and their radical-scavenging activities, and to obtain a sufficiently purified oligophenolic fraction to perform an RP-HPLC analysis on molecules with a molecular weight (MW) < 2,kDa. Methodology , Phenolic compounds were separated and purified by liquid,liquid extraction, tangential ultrafiltration and dialysis. Then, the contents of both phenolic compounds and radical-scavenging activities were measured by the Folin,Ciocalteu reagent, and DPPH and ABTS assays. NMR analysis was performed to validate the process. RP-HPLC with a C18 column was performed on the oligophenolic fraction, using a novel method developed in this study. Results , Seven fractions were obtained as a function of polarity and molecular weight. Among them, the fraction containing phenolic compounds with a MW , 50,kDa appeared to be the most active, correlated with the content of phenolic compounds. Conclusion , This work constitutes a step forward in the separation and purification of bioactive phlorotannins and represents a prerequisite for further investigations into their structural characterisation and distribution in A. nodosum. [source]