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Chemiluminescence Reaction (chemiluminescence + reaction)
Selected AbstractsHighly Sensitive Electrogenerated Chemiluminescence Detecting Ranitidine Based On Chemically Modifying Microenvironment of the Chemiluminescence ReactionELECTROANALYSIS, Issue 11 2005Xingwang Zheng Abstract Using a graphite electrode modified with vaseline and NiO, ranitidine showed a strongly ECL enhancing effect for the weak ECL signal of electrooxidation of luminol. Based on this finding, a more sensitive ECL method for ranitidine was firstly proposed. Under the optimum experimental conditions, the ranitidine hydrochloride concentration in the range of 3.0×10,8,9.0×10,6,mol/L was proportional to the enhancing ECL signal and offered a 9×10,9,mol/L detection limit for ranitidine hydrochloride. At the same time, based on the investigation on this ECL reaction mechanism, a new concept, to improve the suitable ECL reaction micro-environment with chemically modified electrode technique for the better analytical performances of ECL analysis was also firstly proposed. [source] Chemiluminescence-Generating Nanoreactor Formulation for Near-Infrared Imaging of Hydrogen Peroxide and Glucose Level in vivoADVANCED FUNCTIONAL MATERIALS, Issue 16 2010Chang-Keun Lim Abstract Water-dispersed all-in-one nanoprobes composed of densely integrated peroxyoxalate fuel and a cyanine dye are formulated to optimize the nanoscopic chemiluminescence reaction. It is demonstrated that the chemiluminescent nanoformulation can generate bright near-infrared signal in response to external hydrogen peroxide that is biologically implicated with cell signaling and diseases. Successful imaging of endogenously overproduces hydrogen peroxide and indirect determination of glucose level in vivo with the chemiluminescent nanoprobes offers an opportunity for early diagnosis of diseases. [source] Chemiluminescence-Generating Nanoreactor Formulation for Near-Infrared Imaging of Hydrogen Peroxide and Glucose Level in vivoADVANCED FUNCTIONAL MATERIALS, Issue 16 2010Chang-Keun Lim Abstract Water-dispersed all-in-one nanoprobes composed of densely integrated peroxyoxalate fuel and a cyanine dye are formulated to optimize the nanoscopic chemiluminescence reaction. It is demonstrated that the chemiluminescent nanoformulation can generate bright near-infrared signal in response to external hydrogen peroxide that is biologically implicated with cell signaling and diseases. Successful imaging of endogenously overproduces hydrogen peroxide and indirect determination of glucose level in vivo with the chemiluminescent nanoprobes offers an opportunity for early diagnosis of diseases. [source] Chemiluminescence determination of chlorpheniramine using tris(1,10-phenanthroline),ruthenium(II) peroxydisulphate system and sequential injection analysisLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 1 2009Fakhr Eldin O. Suliman Abstract A sequential injection (SI) method was developed for the determination of chlorpheniramine (CPA), based on the reaction of this drug with tris(1,10-phenanthroline),ruthenium(II) [Ru(phen)32+] and peroxydisulphate (S2O82,) in the presence of light. The instrumental set-up utilized a syringe pump and a multiposition valve to aspirate the reagents [Ru(phen)32+ and S2O82,] and a peristaltic pump to propel the sample. The experimental conditions affecting the chemiluminescence reaction were systematically optimized, using the univariate approach. Under the optimum conditions linear calibration curves of 0.1,10 µg/ml were obtained. The detection limit was 0.04 µg/ml and the relative standard deviation (RSD) was always < 5%. The procedure was applied to the analysis of CPA in pharmaceutical products and was found to be free from interferences from concomitants usually present in these preparations. Copyright © 2008 John Wiley & Sons, Ltd. [source] Electrogenerated chemiluminescence reaction of lucigenin with isatin at a platinum electrodeLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 1 2004Honglan Qi Abstract The electrogenerated chemiluminescence (ECL) reaction of lucigenin with isatin was investigated at a platinum electrode in a neutral aqueous solution. The ECL intensity of lucigenin at ,0.65 V was greatly enhanced by isatin, and the ECL intensity was about 50 times higher than that of lucigenin without isatin. The enhanced ECL was believed to be produced by the chemiluminescence reaction between reduced lucigenin and superoxide anion that was generated by the reaction of electrochemically reduced isatin with dissolved oxygen. The conditions for the determination of isatin were optimized. Under the optimized condition, the enhanced ECL intensity vs. isatin concentration was linear in the range 4.8 × 10,7,1.9 × 10,5 g/mL; with a detection limit of 3.3 × 10,8 g/mL, and the relative standard derivation 1.0 × 10,6 g/mL isatin was 3.8%. Copyright © 2004 John Wiley & Sons, Ltd. [source] Kinetic studies on the peroxyoxalate chemiluminescence reaction: determination of the cyclization rate constantLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 5 2002Sandra M. Silva Abstract Although more currently utilized as analytical tool because of its high sensitivity and good reproducibility, the mechanism of the peroxyoxalate system, a chemiluminescence reaction with quantum yields only comparable to bioluminescence systems, has been extensively studied. The light emission mechanism can be divided in the pathway before chemiexcitation, which contains the rate-limiting steps, and the fast and kinetically non-observable chemiexcitation step. In this work, we obtain information on the mechanism of the slow pathways, attribute values to several rate constants prior to chemiexcitation and suggest a mechanistic scheme that could help optimization of conditions when the peroxyoxalate reaction is used as analytical tool. Copyright © 2002 John Wiley & Sons, Ltd. [source] Application of an enhanced luminol chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H -imidazol-2-yl]phenylboronic acid to photographic detection of horseradish peroxidase on a membraneLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 2 2001Naotaka Kuroda Abstract Photographic detection of horseradish peroxidase (HRP) on a membrane by the luminol,hydrogen peroxide,HRP chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H -imidazol-2-yl]phenylboronic acid (DPPA) as an enhancer is described. The method is based on the long-lived chemiluminescence emission obtained by using DPPA. Under the optimum conditions, as little as 0.10,ng (ca. 2.3 fmol) and 0.20,ng (ca. 4.6 fmol) per spot of HRP on a membrane were detected as visible spots with exposure time of 60 and 10,min, respectively, by using an instant photographic film and a camera luminometer. The proposed method was highly sensitive and was successfully applied to the detection of HRP conjugates as an alternative to the colorimetric method using a chromogenic substrate in a commercially available assay kit of Western blotting. Copyright © 2001 John Wiley & Sons, Ltd. [source] Relative distribution of three major lactate transporters in frozen human tissues and their localization in unfixed skeletal muscleMUSCLE AND NERVE, Issue 1 2002William N. Fishbein MD Abstract We have prepared affinity-purified rabbit polyclonal antibodies to the near-C-terminal peptides of human monocarboxylate transporters (MCTs) 1, 2, and 4 coupled to keyhole limpet hemocyanin. Each antiserum reacted only with its specific peptide antigen and gave a distinct molecular weight band (blocked by preincubation with antigen) after chemiluminescence reaction on Western blots from sodium dodecyl sulfate,polyacrylamide gel electrophoresis (SDS-PAGE) of tissue membrane proteins. Densitometry showed distinctive expression patterns for each MCT in a panel of 15 frozen human tissues, with the distribution of MCT1 ,L:MCT2>MCT4. Fluorescence microscopy of unfixed skeletal muscle using fluorescein-conjugated secondary antibody was correlated with reverse adenosine triphosphatase (ATPase) stained sequential sections to identify fiber-type localization. MCT1 expression was high in the sarcolemma of type 1 fibers, modest to low in type 2a fibers, and almost absent in type 2b fibers. In contrast, MCT4 expression was low to absent in the membrane of most type 1 fibers, but high in most 2a and in all 2b fibers, favoring the view that their high lactate levels during work may be channeled in part to neighboring type 1 (and perhaps 2a) fibers for oxidation, thereby delaying fatigue. MCT2 expression was limited to the sarcolemma of a type 1 fiber subset, which varied from <5 to 40%, depending on the specific muscle under study. Quantitative chemiluminescent densitometry of 10 muscle biopsies for their MCT2 and MCT4 content, each normalized to MCT1, confirmed the unique variation of MCT2 expression with biopsy site. The application of these antibodies should add to the understanding of motor unit physiology, and may contribute to the muscle-biopsy assessment of low-level denervation. © 2002 Wiley Periodicals, Inc. Muscle Nerve 26: 101,112, 2002 [source] A Nonenzymatic Chemiluminescent Reaction Enabling Chemiluminescence Resonance Energy Transfer to Quantum DotsCHEMISTRY - A EUROPEAN JOURNAL, Issue 21 2010Shulin Zhao Glowing transfer: An efficient chemiluminescence resonance energy transfer (CRET) between a luminol donor and a quantum dot acceptor was observed for the first time from a nonenzymatic reaction involving luminol,NaBrO,CdTe quantum dots (see figure). This work represents significant progress from the previously reported observation of CRET between a luminol donor and a horseradish peroxidase conjugated quantum dot acceptor from an enzyme-catalyzed chemiluminescence reaction. [source] Determination of Epinephrine by Flow Injection Analysis Coupled Ag(III) Complex-Luminol Chemiluminescence DetectionCHINESE JOURNAL OF CHEMISTRY, Issue 4 2009Jiangbo BAI Abstract A new Ag(III) complex-luminol chemiluminescent system which was applied to the determination of epinephrine is firstly reported. Based on the enhancing effect of epinephrine on the chemiluminescence reaction of luminol with [Ag(HIO6)2]5, in alkaline solution, a highly sensitive chemiluminescence (CL) detection by flow injection analysis (FIA) was developed for epinephrine. Under the optimum conditions, CL intensity was proportional to concentration of epinephrine in the 1.0×10,9,1.0×10,7 mol·L,1 range. The limit of detection was 8.0×10,10 mol·L,1 for epinephrine (3,), with a relative standard deviation (n=11) of 2.9% for 1.5×10,8 mol·L,1 epinephrine. The method validation was done with epinephrine determinations in commercial pharmaceutical products. The mechanism of the reactions was also discussed. [source] | ||||||||||||||||||||||