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Charge-coupled Device (charge-coupled + device)
Selected AbstractsEvaluation of a digital camera image applied to PCB inspectionHUMAN FACTORS AND ERGONOMICS IN MANUFACTURING & SERVICE INDUSTRIES, Issue 4 2008Bernard C. Jiang Rapid advancement and widespread digital camera applications have made it possible to replace charge-coupled device (CCD) cameras in automatic inspections for industrial applications. However, most digital camera applications using the automatic exposure mode may not be effective in some of the inspection environments. The reflection from a board surface in printed circuit board (PCB) inspections is one such problem area. The objective of this study is to develop a methodology to evaluate the effectiveness of using digital cameras for inspection. The indices used for evaluating digital camera image quality are the perceived image quality, the visual resolution, and the noise. An experiment was designed and conducted to determine the optimal camera parameter combination for attaining the best image quality. The desirability function was used to compare various digital camera parameter settings in considering three image quality indices for selecting the best camera-operating conditions. Based on the developed model and the subjective image quality index, the overall image quality improved 9.4% and 13.86%, respectively. The developed methodology can be used to: (a) determine the digital camera image quality, (b) provide an improved model for determining the automatic exposure setting for digital camera designers, and (c) adjust the digital camera parameters for automatic inspection. © 2008 Wiley Periodicals, Inc. [source] Spatially adaptive color filter array interpolation for noiseless and noisy dataINTERNATIONAL JOURNAL OF IMAGING SYSTEMS AND TECHNOLOGY, Issue 3 2007Dmitriy Paliy Abstract Conventional single-chip digital cameras use color filter arrays (CFA) to sample different spectral components. Demosaicing algorithms interpolate these data to complete red, green, and blue values for each image pixel, to produce an RGB image. In this article, we propose a novel demosaicing algorithm for the Bayer CFA. For the algorithm design, we assume that, following the concept proposed in (Zhang and Wu, IEEE Trans Image Process 14 (2005), 2167,2178), the initial interpolation estimates of color channels contain two additive components: the true values of color intensities and the errors that are considered as an additive noise. A specially designed signal-adaptive filter is used to remove this so-called demosaicing noise. This filter is based on the local polynomial approximation (LPA) and the paradigm of the intersection of confidence intervals applied to select varying scales of LPA. This technique is nonlinear and spatially-adaptive with respect to the smoothness and irregularities of the image. The presented CFA interpolation (CFAI) technique takes significant advantage from assuming that the original data is noise-free. Nevertheless, in many applications, the observed data is noisy, where the noise is treated as an important intrinsic degradation of the data. We develop an adaptation of the proposed CFAI for noisy data, integrating the denoising and CFAI into a single procedure. It is assumed that the data is given according to the Bayer pattern and corrupted by signal-dependant noise common for charge-coupled device and complementary-symmetry/metal-oxide semiconductor sensors. The efficiency of the proposed approach is demonstrated by experimental results with simulated and real data. © 2007 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 17, 105,122, 2007 [source] Constrained total least-squares computations for high-resolution image reconstruction with multisensorsINTERNATIONAL JOURNAL OF IMAGING SYSTEMS AND TECHNOLOGY, Issue 1 2002Michael K. Ng Multiple undersampled images of a scene are often obtained by using a charge-coupled device (CCD) detector array of sensors that are shifted relative to each other by subpixel displacements. This geometry of sensors, where each sensor has a subarray of sensing elements of suitable size, has been popular in the task of attaining spatial resolution enhancement from the acquired low-resolution degraded images that comprise the set of observations. With the objective of improving the performance of the signal processing algorithms in the presence of the ubiquitous perturbation errors of displacements around the ideal subpixel locations (because of imperfections in fabrication), in addition to noisy observation, the errors-in-variables or the total least-squares method is used in this paper. A regularized constrained total least-squares (RCTLS) solution to the problem is given, which requires the minimization of a nonconvex and nonlinear cost functional. Simulations indicate that the choice of the regularization parameter influences significantly the quality of the solution. The L-curve method is used to select the theoretically optimum value of the regularization parameter instead of the unsound but expedient trial-and-error approach. The expected superiority of this RCTLS approach over the conventional least-squares theory-based algorithm is substantiated by example. © 2002 John Wiley & Sons, Inc. Int J Imaging Syst Technol 12, 35,42, 2002 [source] Standardization of line-scan NIR imaging systemsJOURNAL OF CHEMOMETRICS, Issue 3-4 2007Zheng Liu Abstract A simple and easy to use method is proposed for standardizing NIR imaging systems for differences among detectors in the charge-coupled device (CCD) array and illumination unevenness. The standardization equations are then used to pre-treat NIR image data to reduce the systematic errors introduced by a line-scan NIR imaging system. The method requires only easily available homogeneous standards with relatively uniform spectral response. The effectiveness of the standardization in reducing the pixel-to-pixel biases and other systematic effects is illustrated with examples, and the improved sensitivity in results obtained from a multivariate image analysis (MIA) based on multi-way principal component analysis (MPCA) is demonstrated. Copyright © 2007 John Wiley & Sons, Ltd. [source] Digital imaging in transmission electron microscopyJOURNAL OF MICROSCOPY, Issue 1 2000G. Y. Fan The digital revolution currently under way, as evidenced by the rapid development of the Internet and the world-wide-web technologies, is undoubtedly impacting the field of transmission electron microscopy (TEM). Digital imaging systems based on charge-coupled device (CCD) technologies, with pixel array size up to 2 k × 2 k at the present and increasing, are available for TEM applications and offer many attractions. Is it time to phase out film cameras on TEMs and close the darkrooms for good? This paper reviews digital imaging technologies for TEM at different voltages, and contrasts the performance of digital imaging systems with that of TEM film. The performance characteristics of CCD-based digital imaging systems, as well as methods for assessing them, are discussed. Other approaches to digital imaging are also briefly reviewed. [source] Application of a microfluidic device for counting of bacteriaLETTERS IN APPLIED MICROBIOLOGY, Issue 3 2006K.-I. Inatomi Abstract Aims:, To develop a miniaturized analytical system for counting of bacteria. Methods and Results:,Escherichia coli cells were used throughout the experiments. The system consists of a microfluidic chamber, a fluorescence microscope with a charge-coupled device (CCD) camera and syringe pumps. The chamber was made of a silicone rubber (30 × 30 mm and 4 mm high). The E. coli cells were flowed from a micro-nozzle fabricated in the chamber and detected with the CCD camera. The individual cells were indicated as signal peaks on a computer. The cell counts showed a good correlation compared with that of a conventional plate counting method, and results of the simultaneous detection of live and dead cells were also presented. Conclusions, Significance and Impact of the Study:, The system having a small disposable nozzle has the advantages for low cost and safe medical or environmental analysis, when compared with a conventional flow cytometer. This is the first step of the development of a one-chip microbe analyzer. [source] Non-enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS deviceLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 5 2008Brian Filanoski Abstract A new method for non-enzymatic aqueous peroxyoxalate chemiluminescence (POCL) biomolecular detection using imaging chip-based devices has been developed. A water-soluble amide of oxalic acid was synthesized and used in the investigation and characterization of POCL immunodetection in an aqueous environment. Six fluorescent dyes commonly used in biological detection were tested, and the intensity of light generated from the aqueous POCL reactions was characterized in the liquid phase. Direct detection sensitivity comparisons between a standard fluorescent method and this POCL method were performed in both liquid and solid phases. Results showed that detection sensitivity using the POCL method is comparable to that of the fluorescent method. POCL biomolecular detection on a nitrocellulose membrane was also investigated using a charge-coupled device (CCD) camera. Again, POCL detection sensitivity proved to be comparable to that using the fluorescent detection method. In an application of aqueous POCL biomolecular detection, Staphylococcus aureus enterotoxin B (SEB) and its antibody were used to demonstrate immuno- and affinity detection. For further applications, such as DNA and protein arrays, simultaneous detection of biomolecules labelled with different fluorescent labels was investigated, using a complementary metal oxide semiconductor (CMOS) colour imaging chip. Copyright © 2008 John Wiley & Sons, Ltd. [source] Micro-fabrication and monitoring of three-dimensional microstructures based on laser-induced thermoplastic formationMICROSCOPY RESEARCH AND TECHNIQUE, Issue 10 2009Leyan Wang Abstract This article reports a novel laser-induced micro-fabrication method and its monitoring system for three-dimensional (3D) microstructures. The mechanism of the method is that a small zone of thermoplastic material melted by laser heating grows in liquid surrounding environment, solidifying into a convex microstructure, such as micro-dot or micro-pillar. A laser diode (808 nm) with maximum power output of 130 mW is used as power source, and a kind of paraffin mixed with stearic acid and paint serves as the thermoplastic material for 3D microstructure formation experiments. A light microscope system consisting of a charge-coupled device (CCD) and a computer is utilized to realize real-time observation of the micro-fabricating process. The distribution of local temperature rise on material surface created by laser irradiation is simulated. The effects of liquid environment on microstructure formation have been theoretically analyzed and experimentally studied. Experiments are further carried out to investigate the relationship between laser spot and fabricated microstructures. The results indicate that the widths of micro-dots or micro-pillars are mostly determined by the size of focal spot, and their heights increase with the enlargement of laser power density. With this method, a micro-dot array of Chinese characters meaning "China" has been successfully fabricated through computer programming. This method has the advantages of implementing direct, mask-less, real-time and inexpensive 3D microstructure fabrication. Therefore, it would be widely applied in the fields of micro/nano-technology for practical fabrication of different kinds of 3D microstructures. Microsc. Res. Tech., 2009. © 2009 Wiley-Liss, Inc. [source] Deep H, imagery of the Eridanus shellsMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 1 2001P. Boumis A deep H, image of interlocking filamentary arcs of nebulosity has been obtained with a wide-field (,30° diameter) narrow-band filter camera combined with a charge-coupled device as a detector. The resultant mosaic of images, extending to a galactic latitude of ,65°, has been corrected for field distortions and had galactic coordinates superimposed on it to permit accurate correlations with the most recent H i (21 cm), X-ray (0.75 keV) and FIR (IRAS 100 ,m) maps. Furthermore, an upper limit of 0.13 arcsec yr,1 to the expansion proper motion of the primary 25° long nebulous arc has been obtained by comparing a recent H, image obtained with the San Pedro Martir telescope of its filamentary edge with that on a Palomar Observatory Sky Survey E plate obtained in 1951. It is concluded that these filamentary arcs are the superimposed images of separate shells (driven by supernova explosions and/or stellar winds) rather than the edges of a single ,superbubble' stretching from Barnard's Arc (and the Orion Nebula) to these high galactic latitudes. The proper motion measurement argues against the primary H,-emitting arc being associated with the giant radio loop (Loop 2) except in extraordinary circumstances. [source] Long-term tracing of adenoviral expression in rat and rabbit using luciferase imagingTHE JOURNAL OF GENE MEDICINE, Issue 6 2005Jin Zhong Li Abstract Background Luciferase optical imaging provides a novel method to monitor transgene expression in small living animals. As the genetic and immunological heritages of particular animals significantly affect the expression of adenovirus-delivered transgenes, it is essential to know the expression patterns specific to athymic nude and Sprague-Dawley rats, two strains commonly used in rodent models. In this study we set out to determine these patterns. At the same time, we tested luciferase optical imaging in a larger animal, the rabbit. Methods A recombinant luciferase adenoviral vector was injected subcutaneously or intramuscularly into athymic nude rats, Sprague-Dawley rats, and Dutch Belted rabbits. The luciferase expression was assessed using a cooled charge-coupled device. Results The luminescent signal was capable of passing through at least 1.3 cm of muscle tissue and proved to be much stronger when luciferin was delivered via a local injection than by an intraperitoneal injection. Although the types of immune cells differed between immunodeficient and immunocompetent rats, similar amounts and patterns of luciferase expression were observed in the musculature in two rat strains during the 1st month after a viral intramuscular injection. The duration of luciferase expression was longer than 15 months in athymic nude rats, 9 months in Sprague-Dawley rats, and 6 months in rabbits following a direct viral injection. Conclusions Luciferase expression after adenoviral gene delivery can persist for longer than 6 months, even in immunocompetent animals. Live imaging of luciferase expression can be performed not only in small animals, but also in larger animals such as rabbits. Copyright © 2005 John Wiley & Sons, Ltd. [source] Microfabricated arrays of cylindrical wells facilitate single-molecule enzymology of ,-chymotrypsinBIOTECHNOLOGY PROGRESS, Issue 4 2009Angela Y. Chen Abstract Single-molecule enzymology allows scientists to examine the distributions of kinetic rates among members of a population. We describe a simple method for the analysis of single-molecule enzymatic kinetics and provide comparisons to ensemble-averaged kinetics. To isolate our model enzyme, ,-chymotrypsin, into single molecules, we use an array of cylindrical poly(dimethylsiloxane) wells 2 ,m in diameter and 1.35 ,m in height. Inside the wells, a protease assay with a profluorescent substrate detects ,-chymotrypsin activity. We hold the concentration of ,-chymotrypsin at 0.39 nM in a given well with an enzyme-to-substrate ratio of 1:6,666 molecules. Fluorescence emitted by the substrate is proportional to enzyme activity and detectable by a charge-coupled device. This method allows for the simultaneous real-time characterization of hundreds of individual enzymes. We analyze single-molecule kinetics by recording and observing their intensity trajectories over time. By testing our method with our current instruments, we confirm that our methodology is useful for the analysis of single enzymes for extracting static inhomogeneity. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 [source] Electrochemical Modulation of Remote Fluorescence Imaging at an Ordered Opto-electrochemical Nanoaperture ArrayCHEMPHYSCHEM, Issue 8 2004Arnaud Chovin Abstract An array of nanometer-sized apertures capable of electrochemically modulating the fluorescence of a model analyte is presented. The device, which combines near-field optical methods and ultramicroelectrode properties in an array format, is based on an etched coherent optical fiber bundle. Indeed, the fabrication steps produced an ordered array where each optical nanoaperture is surrounded by a ring-shaped gold nanoelectrode. The chronoamperometric behavior of the array shows stable diffusion-limited quasi-steady-state response. The model analyte, tris(2,2,-bipyridine) ruthenium, emits fluorescence in the Ru(II) state, but not in the oxidized Ru(III) state. Fluorescence is excited by visible light exiting from each nanoaperture since light is confined to the tip apex by the gold coating. A fraction of the isotropically emitted luminescence is collected by the same nanoaperture, transmitted by the corresponding fiber core and eventually detected by a charge-coupled device (CCD) camera. The array format provides a fluorescence image resolved at the nanometric scale which covers a large micrometric area. Therefore the high-density array plays a bridging role between these two fundamental scales. We established that the opto-electrochemical nanoapertures are optically independent. Fluorescence of the sample collected by each nanoaperture is modulated by changing the potential of the nanoring electrodes. Reversible electrochemical switching of remote fluorescence imaging is performed through the opto-electrochemical nanoaperture array itself. Eventually this ordered structure of nanometer light sources which are electrochemically manipulated provides promising photonic or electro-optical devices for various future applications. For example, such an array has potential in the development of a combined SNOM-electrochemical nanoprobe array to image a real sample concomitantly at the nanometer and micrometer scale. [source] | ||||||||||||||||