Home About us Contact
|
Home About us Contact | ||
|
|
||
Channel Surfaces (channel + surface)
Selected AbstractsMicrochip-based small, dense low-density lipoproteins assay for coronary heart disease risk assessmentELECTROPHORESIS, Issue 9 2008Hua Wang Abstract Small, dense low-density lipoprotein (sdLDL) has been accepted as an emerging cardiovascular risk factor, and there has been an increasing interest in analytical methods for sdLDL profiling for diagnosis. Serum sdLDL may be measured by different laboratory techniques, but all these methods are laborious, time-consuming, and costly. Recently, we have demonstrated that a low-temperature bonding of quartz microfluidic chips for serum lipoproteins analysis (Zhuang, G., Jin, Q., Liu, J., Cong, H. et al., Biomed. Microdevices 2006, 8, 255,261). In contrast to this previous study, we chose SDS as anionic surfactant to modify both lipoproteins and the channel surface to minimize lipoprotein adsorption and improve the resolution of lipoprotein separation. Two major LDL subclass patterns including large, buoyant LDL (lLDL), sdLDL, and high-density lipoprotein (HDL) were effectively separated with high reproducibility. RSD values of the migration time (min) and peak areas of standard LDL and HDL were 6.28, 4.02, 5.02, and 2.5%, respectively. Serum lipoproteins of 15 healthy subjects and 15 patients with coronary heart disease (CHD) were separated by microchip CE. No peaks of sdLDL were detected in serum samples of healthy subjects while sdLDL fractional peaks were observed in patients' entire serum samples. These results suggested that the microchip-based sdLDLs assay was a simple, rapid, and highly efficient technique and significantly improved the analysis of CHD risk factors. [source] The effect of condenser heat transfer on the energy performance of a plate heat pipe solar collectorINTERNATIONAL JOURNAL OF ENERGY RESEARCH, Issue 10 2005Jorge Facão Abstract For a novel prototype solar collector, using a plate heat pipe, condenser heat transfer was analysed in detail. The condenser has the shape of a rectangular channel. Flow and heat transfer of water in the rectangular channel was modelled and the heat transfer coefficient assessed, using the Fluent code. Under typical operating conditions a mixed convection situation occurs. The channel is inclined and heating is through one wall only (upper channel surface). The range of temperature differences considered was similar to the one verified under real operating conditions, covering a wide range of Grashof numbers. Results showed that the Nusselt number is significantly higher than the one for forced convection in a rectangular channel with fully developed boundary layers. In order to enhance heat transfer, a modification to the rectangular channel was analysed, using baffles to improve flow distribution and increase velocity. The effect of this modification on collector energy performance (efficiency) was assessed. Copyright © 2005 John Wiley & Sons, Ltd. [source] Coarse sediment transport in mountain streams in Colorado and Wyoming, USAEARTH SURFACE PROCESSES AND LANDFORMS, Issue 3 2005Sandra E. Ryan Abstract Since the early 1990s, US Forest Service researchers have made thousands of bedload measurements in steep, coarse-grained channels in Colorado and Wyoming, USA. In this paper we use data from 19 of those sites to characterize patterns and rates of coarse sediment transport for a range of channel types and sizes, including step,pool, plane-bed, pool,riffle, and near-braided channels. This effort builds upon previous work where we applied a piecewise regression model to (1) relate flow to rates of bedload transport and (2) define phases of transport in coarse-grained channels. Earlier, the model was tested using bedload data from eight sites on the Fraser Experimental Forest near Fraser, Colorado. The analysis showed good application to those data and to data from four supplementary channels to which the procedure was applied. The earlier results were, however, derived from data collected at sites that, for the most part, have quite similar geology and runoff regimes. In this paper we evaluate further the application of piecewise regression to data from channels with a wider range of geomorphic conditions. The results corroborate with those from the earlier work in that there is a relatively narrow range of discharges at which a substantial change in the nature of bedload transport occurs. The transition from primarily low rates of sand transport (phase I) to higher rates of sand and coarse gravel transport (phase II) occurs, on average, at about 80 per cent of the bankfull (1·5-year return interval) discharge. A comparison of grain sizes moved during the two phases showed that coarse gravel is rarely trapped in the samplers during phase I transport. Moreover, the movement and capture of the D16 to D25 grain size of the bed surface seems to correspond with the onset of phase II transport, particularly in systems with largely static channel surfaces. However, while there were many similarities in observed patterns of bedload transport at the 19 studied sites, each had its own ,bedload signal' in that the rate and size of materials transported largely reflected the nature of flow and sediment particular to that system. Published in 2005 by John Wiley & Sons, Ltd. [source] A robust cross-linked polyacrylamide coating for microchip electrophoresis of dsDNA fragmentsELECTROPHORESIS, Issue 19 2006Joann J. Lu Abstract Surface derivatization plays an important role in microchip electrophoresis. It not only enhances the resolution, but also improves the reproducibility. So far, the most popularly used derivatization method for glass microchannels is to covalently attach a layer of linear polyacrylamide,(LPA) to the channel surfaces. However, LPA coating has two problems: incomplete coverage and limited lifetime. To address these issues, we have recently developed a cross-linked polyacrylamide,(CPA) derivatization protocol and demonstrated it for high-resolution protein separations by CIEF, CGE, and CZE. In this report, we used this protocol to coat microchip channels and exhibited the reliability and robustness of CPA coating for microchip electrophoresis of DNA molecules. dsDNA fragments were used as our test samples. High resolutions were obtained for fragments ranging from 100,bp to 10,kpb. After more than 800,runs, the CPA-coated microchannels still performed well and comparable resolutions were maintained throughout these runs. [source] Perfusion seeding of channeled elastomeric scaffolds with myocytes and endothelial cells for cardiac tissue engineeringBIOTECHNOLOGY PROGRESS, Issue 2 2010Robert Maidhof Abstract The requirements for engineering clinically sized cardiac constructs include medium perfusion (to maintain cell viability throughout the construct volume) and the protection of cardiac myocytes from hydrodynamic shear. To reconcile these conflicting requirements, we proposed the use of porous elastomeric scaffolds with an array of channels providing conduits for medium perfusion, and sized to provide efficient transport of oxygen to the cells, by a combination of convective flow and molecular diffusion over short distances between the channels. In this study, we investigate the conditions for perfusion seeding of channeled constructs with myocytes and endothelial cells without the gel carrier we previously used to lock the cells within the scaffold pores. We first established the flow parameters for perfusion seeding of porous elastomer scaffolds using the C2C12 myoblast line, and determined that a linear perfusion velocity of 1.0 mm/s resulted in seeding efficiency of 87% ± 26% within 2 hours. When applied to seeding of channeled scaffolds with neonatal rat cardiac myocytes, these conditions also resulted in high efficiency (77.2% ± 23.7%) of cell seeding. Uniform spatial cell distributions were obtained when scaffolds were stacked on top of one another in perfusion cartridges, effectively closing off the channels during perfusion seeding. Perfusion seeding of single scaffolds resulted in preferential cell attachment at the channel surfaces, and was employed for seeding scaffolds with rat aortic endothelial cells. We thus propose that these techniques can be utilized to engineer thick and compact cardiac constructs with parallel channels lined with endothelial cells. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010 [source] | ||||||||||