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Acquity UPLC BEH C18 Column (acquity + uplc_beh_c18_column)
Selected AbstractsDevelopment and validation of UPLC-MS/MS method for simultaneous determination of gestodene and ethinyl estradiol in rat plasmaBIOMEDICAL CHROMATOGRAPHY, Issue 2 2010Zhili Xiong Abstract A selective and sensitive ultra-performance liquid chromatography method with tandem mass spectrometric detection for simultaneous determination of gestodene (GES) and ethinyl estradiol (EE) in rat plasma was developed and validated. GES, EE and the internal standard, norgestrel, were extracted with ethyl acetate, derivatized (EE only) with dansyl chloride and then back-extracted into diethyl ether-hexane (2:1, v/v). The separation was performed on an ACQUITY UPLCÔ BEH C18 column with gradient elution using mobile phase consisting of acetonitrile and water (both containing 0.1% formic acid). The detection was carried out by means of electrospray ionization (ESI) mass spectrometry in positive ion mode with multiple-reaction monitoring. Calibration curves of GES and EE were linear (r2,,,0.99) over the concentration ranges 1.59,159 and 0.196,78.4,ng/mL, respectively. The intra- and inter-day precisions were not more than 6.9 and 12.9% for GES and 10.6 and 9.0% for EE, and the accuracies were ,2.5,8.0% for GES, and ,7.2,0.19% for EE, respectively. The method herein described was superior to previous methods and was applicable to the pharmacokinetic study of GES and EE in rats. Copyright © 2009 John Wiley & Sons, Ltd. [source] Determination of multicomponent contents in Calculus bovis by ultra-performance liquid chromatography,evaporative light scattering detection and its application for quality controlJOURNAL OF SEPARATION SCIENCE, JSS, Issue 10 2010Weijun Kong Abstract A fast ultra-performance liquid chromatography,evaporative light scattering detection (UPLC-ELSD) method was established for simultaneous quantification of seven components in natural Calculus bovis (C. bovis) and its substitutes or spurious breeds. On a Waters Acquity UPLC® BEH C18 column, seven analytes were efficiently separated using 0.2% aqueous formic acid,acetonitrile as the mobile phase in a gradient program. The evaporator tube temperature of ELSD was set at 100°C with the nebulizing gas flow-rate of 1.9,L/min. The results showed that this established UPLC-ELSD method was validated to be sensitive, precise and accurate with the LODs of seven analytes at 2,11,ng, and the overall intra-day and inter-day variations less than 3.0%. The recovery of the method was in the range of 97.8,101.6%, with RSD less than 3.0%. Further results of PCA on the contents of seven investigated analytes suggested that compounds of cholic acid, deoxycholic acid and chenodeoxycholic acid or cholesterol should be added as chemical markers to UPLC analysis of C. bovis samples for quality control and to discriminate natural C. bovis sample and its substitutes or some spurious breeds, then normalize the use of natural C. bovis and ensure its clinical efficacy. [source] Determination of quinapril and quinaprilat in human plasma by ultraperformance liquid chromatography,electrospray ionization mass spectrometryBIOMEDICAL CHROMATOGRAPHY, Issue 5 2009Bhavesh Dasandi Abstract A novel, specific and sensitive ultraperformance liquid chromatography tandem mass spectrometry (UPLC,MS/MS) method was developed for the simultaneous determination of quinapril and its active metabolite quinaprilat in human plasma. The method involves a simple, one-step extraction procedure coupled with an Acquity UPLCÔ BEH C18 column (100 × 2.1 mm, i.d., 1.7 µm) with isocratic elution at a flow-rate of 0.2 mL/min and lisinopril as the internal standard. Detection was performed on a triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode via electrospray ionization. Using 250 µL plasma, the methods were validated over the concentration range 5.010,500.374 ng/mL for quinapril and 10.012,1000 ng/mL for quinaprilat, with a lower limit of quantification of 5.010 ng/mL for quinapril and 10.012 ng/mL for quinaprilat. The intra- and inter-day precision and accuracy were within 10.0%. The recovery was 85.8, 62.6 and 61.3% for quinapril, quinaprilat and lisinopril, respectively. Total run time was 3.0 min only. Copyright © 2008 John Wiley & Sons, Ltd. [source] Simultaneous determination of udenafil and its active metabolite, DA-8164, in human plasma and urine using ultra-performance liquid chromatography,tandem mass spectrometry: application to a pharmacokinetic studyBIOMEDICAL CHROMATOGRAPHY, Issue 9 2008Soo Kyung Bae Abstract A rapid, sensitive, and simple ultra-performance liquid chromatography,tandem mass spectrometry (UPLC/MS/MS) method for the determination of udenafil and its active metabolite, DA-8164, in human plasma and urine using sildenafil as an internal standard (IS) was developed and validated. Udenafil, DA-8164 and IS from a 100 µL aliquot of biological samples were extracted by protein precipitation using acetonitrile. Chromatographic separation was carried on an Acquity UPLC BEH C18 column (50 × 2.1 mm, i.d., 1.7 µm) with an isocratic mobile phase consisting of acetonitrile and containing 0.1% formic acid (75:25, v/v) at flow rate of 0.4 mL/min, and total run time was within 1 min. Detection and quantification was performed by the mass spectrometer using multiple reaction-monitoring mode at m/z 517 , 283 for udenafil, m/z 406 , 364 for DA-8164 and m/z 475 , 100 for IS. The assay was linear over a concentration range of 1,600 ng/mL with a lower limit of quantification of 1 ng/mL in both human plasma and urine. The coefficient of variation of this assay precision was less than 13.7%, and the accuracy exceeded 92.0%. This method was successfully applied for pharmacokinetic study after oral administration of udenafil 100 mg to healthy Korean male volunteers. Copyright © 2008 John Wiley & Sons, Ltd. [source] Simultaneous determination and pharmacokinetic study of oxymatrine and matrine in beagle dog plasma after oral administration of Kushen formula granule, oxymatrine and matrine by LC-MS/MSBIOMEDICAL CHROMATOGRAPHY, Issue 8 2007Yiqi Wang Abstract A rapid, specific and sensitive LC-MS/MS method was developed for the determination of oxymatrine (OMT) and matrine (MT) in beagle dog plasma. The method was applied to study the pharmacokinetics of OMT and MT after oral administration of OMT, MT and Kushen formula granule (KFG) containing equivalent amounts of OMT and MT in a three-period crossover design. The analysis was carried out on an Acquity UPLCÔ BEH C18 column by linear gradient elution with 0.01% acetic acid,water,methanol as mobile phase. Detection was by positive ion electrospray ionization (ESI) mass spectrometry with multiple-reaction monitoring (MRM). Linear calibration curves were both obtained over the concentration range 15,2000 ng/mL, with a limit of quantification of 15 ng/mL. The matrix effect was minimized. The intra- and inter-day precisions (RSDs) were less than 12.4 and 14.7%, respectively, and the accuracy (RE) was from ,2.1 to 2.7%. The validated method was used to determine the concentration,time profiles of OMT and MT. The results indicated that the absorption of OMT and MT after oral administration of KFG was significantly greater than that after oral administration of pure components. Copyright © 2007 John Wiley & Sons, Ltd. [source] | ||||||||||