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Acid Retention (acid + retention)
Selected AbstractsQuality Retention in Strawberry and Carrot Purees Dried with Refractance WindowTM SystemJOURNAL OF FOOD SCIENCE, Issue 3 2002B.I. Abonyi ABSTRACT: The quality retention characteristics of strawberry and carrot purees dried using the Refractance WindowTM (RW) drying method were evaluated against freeze drying, drum drying, and spray drying methods. Ascorbic acid retention of the strawberry purees (94.0%) after RW drying was comparable to 93.6% in freeze-drying. The carotene losses for RW drying were 8.7% (total carotene), 7.4% (,-carotene), and 9.9% (,-carotene), which were comparable to losses of 4.0% (total carotene), 2.4% (,-carotene), and 5.4% (,-carotene) for freeze-dried carrot purees. The color of the RW-dried carrot purees was comparable to fresh puree. For RW-dried strawberry purees, the color retention was comparable to freeze-dried products. RW drying altered the overall perception of aroma in strawberries. [source] Growth, nitrogen gain and indispensable amino acid retention of pacu (Piaractus mesopotamicus, Holmberg 1887) fed different brewers yeast (Saccharomyces cerevisiae) levelsAQUACULTURE NUTRITION, Issue 3 2010R.O.A. OZÓRIO Abstract A feeding-and-digestibility trials were carried out to evaluate the efficacy of replacing fishmeal with brewers yeast Saccharomyces cerevisiae in diets of pacu, Piaractus mesopotamicus, juveniles. The feeding trial was conducted during 54 days with 450 fish (26.6 ± 1.7 g) testing six isonitrogenous (270 g kg,1 crude protein) and isoenergetic (19 MJ kg,1 crude energy) diets, with increasing yeast level to replace 0 (control), 30, 35, 50, 70 or 100% of dietary fishmeal. Growth performance and feed utilization increased with increasing dietary yeast level until 50% fishmeal replacement. Protein retention efficiency was higher in fish fed 35 and 50%. Protein digestibility and the fillet hue (the red/green chromaticity) were not significantly different among all treatments. Nitrogen gains were significantly improved in fish fed 35% replacement diet compared to fish fed the control diet. The retentions of indispensable amino acids tended to increase with increasing dietary yeast levels, with maximum retention at 35,50%. On the basis of our results, replacing 50% fishmeal by yeast in pacu diets successfully improved feed efficiency and growth performance, and reduced nitrogen losses, thereby reducing the nitrogen outputs from fish farms. [source] Effects of alternate phases of fish oil and vegetable oil-based diets in Murray codAQUACULTURE RESEARCH, Issue 10 2009David S Francis Abstract Fish oil (FO)- and canola oil (CO)-based diets were regularly alternated in a daily cycle (amCO: alternation of CO in the morning and FO in the afternoon, and pmCO: alternation of FO in the morning and CO in the afternoon) or in a series of weekly cycles (2W: alternation of 2 weeks on CO and 2 weeks on FO, 4W: alternation of 4 weeks on CO and 4 weeks on FO), over a 16-week period in juvenile Murray cod (Maccullochella peelii peelii). No significant differences were observed between any of the treatments in relation to the final weight. However, fish subjected to the 2W schedule were larger (P>0.05) than all other treatments (37.2 ± 0.30 vs. 34.3 ± 0.58 in the control treatment). Fish receiving the 2W treatment had a significantly lower total net disappearance of eicosapentaenoic acid 20:5n-3 (EPA) and docosahexaenoic acid 22:6n-3 (62.1% and 24.0% respectively) compared with the control treatment (fish continuously fed a blend of 50% FO and 50% CO). Likewise, Murray cod receiving the amCO daily schedule had a significantly lower total net disappearance of EPA in comparison with the CD and pmCO treatments. These data point towards the existence of cyclical mechanisms relative to fatty acid utilization/retention. [source] Differential interactions of plasmid DNA, RNA and genomic DNA with amino acid-based affinity matricesJOURNAL OF SEPARATION SCIENCE, JSS, Issue 17-18 2010Angela Sousa Abstract The development of a strategy to plasmid DNA (pDNA) purification has become necessary for the development of gene therapy and DNA vaccine production processes in recent years, since this nucleic acid and most of contaminants, such as RNA, genomic DNA and endotoxins, are negatively charged. An ideal separation methodology may be achieved with the use of affinity interactions between immobilized amino acids and nucleic acids. In this study, the binding behaviour of nucleic acids under the influence of different environmental conditions, such as the composition and ionic strength of elution buffer, and the temperature, is compared with various amino acids immobilized on chromatography resins. Supercoiled (sc) plasmid isoform was isolated with all matrices used, but in some cases preferential interactions with other nucleic acids were found. Particularly, lysine chromatography showed to be an ideal technology mainly on RNA purification using low salt concentration. On the other hand, arginine ligands have shown a greater ability to retain the sc isoform comparatively to the other nucleic acids retention, becoming this support more adequate to sc pDNA purification. The temperature variation, competitive elution and oligonucleotides affinity studies also allowed to recognize the dominant interactions inherent to biorecognition of pDNA molecule and the affinity matrices. [source] | |||||||||||||