|
| ||
|
|
||
Cell Scaffold (cell + scaffold)
Selected AbstractsFabrication and surface modification of macroporous poly(L -lactic acid) and poly(L -lactic- co -glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell cultureJOURNAL OF BIOMEDICAL MATERIALS RESEARCH, Issue 3 2002Jian Yang Abstract The fabrication and surface modification of a porous cell scaffold are very important in tissue engineering. Of most concern are high-density cell seeding, nutrient and oxygen supply, and cell affinity. In the present study, poly(L -lactic acid) and poly(L -lactic- co -glycolic acid) (70/30) cell scaffolds with different pore structures were fabricated. An improved method based on Archimedes' Principle for measuring the porosity of scaffolds, using a density bottle, was developed. Anhydrous ammonia plasma treatment was used to modify surface properties to improve the cell affinity of the scaffolds. The results show that hydrophilicity and surface energy were improved. The polar N-containing groups and positive charged groups also were incorporated into the sample surface. A low-temperature treatment was used to maintain the plasma-modified surface properties effectively. It would do help to the further application of plasma treatment technique. Cell culture results showed that pores smaller than 160 ,m are suitable for human skin fibroblast cell growth. Cell seeding efficiency was maintained at above 99%, which is better than the efficiency achieved with the common method of prewetting by ethanol. The plasma-treatment method also helped to resolve the problem of cell loss during cell seeding, and the negative effects of the ethanol trace on cell culture were avoided. The results suggest that anhydrous ammonia plasma treatment enhances the cell affinity of porous scaffolds. Mass transport issues also have been considered. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 438,446, 2002 [source] Electrospun Alginate Nanofibers with Controlled Cell Adhesion for Tissue Engineering,MACROMOLECULAR BIOSCIENCE, Issue 8 2010Sung In Jeong Abstract Alginate, a natural polysaccharide that has shown great potential as a cell scaffold for the regeneration of many tissues, has only been nominally explored as an electrospun biomaterial due to cytotoxic chemicals that have typically been used during nanofiber formation and crosslinking. Alginate cannot be electrospun by itself and is often co-spun with poly(ethylene oxide) (PEO). In this work, a cell adhesive peptide (GRGDSP) modified alginate (RA) and unmodified alginate (UA) were blended with PEO at different concentrations and blending ratios, and then electrospun to prepare uniform nanofibers. The ability of electrospun RA scaffolds to support human dermal fibroblast cell attachment, spreading, and subsequent proliferation was greatly enhanced on the adhesion ligand-modified nanofibers, demonstrating the promise of this electrospun polysaccharide material with defined nanoscale architecture and cell adhesive properties for tissue regeneration applications. [source] Enhancing the cell affinity of macroporous poly(L -lactide) cell scaffold by a convenient surface modification methodPOLYMER INTERNATIONAL, Issue 12 2003Jian Yang Abstract In this study, the macroporous poly(L -lactide) (PLLA) cell scaffold was modified for enhancing its cell affinity by an improved surface-treating medium, a mixture of aqueous 0.25 M NaOH/ethanol. Ethanol was applied as a co-treating medium to wet the polylactone and assist the hydroxide nucleophilic attack on the ester bond. Low concentration of NaOH could be applied, severe bulk degradation could be avoided and the residual alkali was easy to remove. Treating time could also be shortened. After treatment under optimal conditions, the surface hydrophilicity and surface energy of PLLA were improved significantly and the surface roughness was also changed. Modification of the spherulite structure on PLLA surface was observed with the treating time using a computer-assisted image analysis system (CAIAS). The results of gel permeation chromatography measurements indicated that only the outer layer of the PLLA was modified and the bulk properties were not altered. Mouse 3T3 fibroblasts culture results indicated that the improved surface-treating medium was effective and convenient for enhancing the cell affinity of PLLA cell scaffold. Copyright © 2003 Society of Chemical Industry [source] Fabrication and surface modification of macroporous poly(L -lactic acid) and poly(L -lactic- co -glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell cultureJOURNAL OF BIOMEDICAL MATERIALS RESEARCH, Issue 3 2002Jian Yang Abstract The fabrication and surface modification of a porous cell scaffold are very important in tissue engineering. Of most concern are high-density cell seeding, nutrient and oxygen supply, and cell affinity. In the present study, poly(L -lactic acid) and poly(L -lactic- co -glycolic acid) (70/30) cell scaffolds with different pore structures were fabricated. An improved method based on Archimedes' Principle for measuring the porosity of scaffolds, using a density bottle, was developed. Anhydrous ammonia plasma treatment was used to modify surface properties to improve the cell affinity of the scaffolds. The results show that hydrophilicity and surface energy were improved. The polar N-containing groups and positive charged groups also were incorporated into the sample surface. A low-temperature treatment was used to maintain the plasma-modified surface properties effectively. It would do help to the further application of plasma treatment technique. Cell culture results showed that pores smaller than 160 ,m are suitable for human skin fibroblast cell growth. Cell seeding efficiency was maintained at above 99%, which is better than the efficiency achieved with the common method of prewetting by ethanol. The plasma-treatment method also helped to resolve the problem of cell loss during cell seeding, and the negative effects of the ethanol trace on cell culture were avoided. The results suggest that anhydrous ammonia plasma treatment enhances the cell affinity of porous scaffolds. Mass transport issues also have been considered. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 438,446, 2002 [source] | ||||||||||