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Cannabis Sativa L. (cannabis + sativa_l)
Selected AbstractsFunctional and structural properties and in vitro digestibility of acylated hemp (Cannabis sativa L.) protein isolatesINTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 12 2009Shou-Wei Yin Summary The effects of succinylation and acetylation on some functional, structural properties and in vitro trypsin digestibility of hemp protein isolate (HPI) were investigated. The extent of acylation gradually increased from 0 to 60,70%, with the anhydride-to-protein ratio increasing from 0 to 1.0 g g,1. Size exclusion chromatography showed that succinylation led to formation of more soluble protein aggregate than acetylation, especially at anhydride levels higher than 0.1 g g,1. Succinylation led to gradual increase in protein solubility (PS) from 30 to 85,90%, while in the acetylation case, the PS was improved only at low anhydride levels, increasing from 30 to about 50% with anhydride-to-protein ratio increasing from 0 to 0.2 g g,1. At neutral pH, the emulsifying activity indexes (EAI) of HPI was 22.1 m2 g,1, and the EAI linearly and significantly increased with the extent of acylation. The EAIs of succinylated and acetylated HPI (1.0 g g,1) were 119.0 and 54.4 m2 g,1, respectively. Differential scanning calorimetry (DSC) and intrinsic fluorescence spectrum analyses indicated gradual structural unfolding of proteins, or exposure of hydrophobic clusters to the solvent, especially at higher anhydride levels. Additionally, the in vitro trypsin digestibility was significantly improved by the succinylation. The results indicated that the chemical acylation treatment (especially succinylation) could be applied to modify some selected functional properties of hemp proteins, especially PS and emulsifying ability. [source] Isolation of microsatellite markers in Cannabis sativa L. (marijuana)MOLECULAR ECOLOGY RESOURCES, Issue 1 2003S. Gilmore Abstract We have identified 15 variable microsatellite loci in Cannabis sativa. In 48 samples from five fibre crop seed accessions, we detected an average of 10 alleles per locus (range 2,28) with mean heterozygosity of 0.68 (range 0.28,0.94). Significant genetic differentiation was found between accessions (FST = 0.12, P < 0.001). These markers have utility for characterizing genetic diversity in cultivated and naturalized Cannabis populations. [source] Plant cannabinoids: a neglected pharmacological treasure troveBRITISH JOURNAL OF PHARMACOLOGY, Issue 7 2005Raphael Mechoulam Most of the cannabinoids in Cannabis sativa L. have not been fully evaluated for their pharmacological activity. A publication in this issue presents evidence that a plant cannabinoid, ,9 -tetrahydrocannabivarin is a potent antagonist of anandamide, a major endogenous cannabinoid. It seems possible that many of the non-psychoactive constituents of this plant will be of biological interest. British Journal of Pharmacology (2005) 146, 913,915. doi:10.1038/sj.bjp.0706415 [source] History of Cannabis and Its Preparations in Saga, Science, and SobriquetCHEMISTRY & BIODIVERSITY, Issue 8 2007Ethan Abstract Cannabis sativa L. is possibly one of the oldest plants cultivated by man, but has remained a source of controversy throughout its history. Whether pariah or panacea, this most versatile botanical has provided a mirror to medicine and has pointed the way in the last two decades toward a host of medical challenges from analgesia to weight loss through the discovery of its myriad biochemical attributes and the endocannabinoid system wherein many of its components operate. This study surveys the history of cannabis, its genetics and preparations. A review of cannabis usage in Ancient Egypt will serve as an archetype, while examining first mentions from various Old World cultures and their pertinence for contemporary scientific investigation. Cannabis historians of the past have provided promising clues to potential treatments for a wide array of currently puzzling medical syndromes including chronic pain, spasticity, cancer, seizure disorders, nausea, anorexia, and infectious disease that remain challenges for 21st century medicine. Information gleaned from the history of cannabis administration in its various forms may provide useful points of departure for research into novel delivery techniques and standardization of cannabis-based medicines that will allow their prescription for treatment of these intractable medical conditions. [source] | ||||||||||