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Cavity Formation (cavity + formation)
Selected AbstractsDifferential regulation of GDF-5 and FGF-2/4 by immobilisation in ovo exposes distinct roles in joint formation,DEVELOPMENTAL DYNAMICS, Issue 3 2006E. Kavanagh Abstract Members of the fibroblast growth factor (FGF) family and growth and differentiation factor 5 (GDF-5) have been implicated in joint specification, but their roles in subsequent cavity formation are not defined. Cavity formation (cavitation) depends upon limb movement in embryonic chicks and factors involved in joint formation are often identified by their expression at the joint-line. We have sought support for the roles of FGF-2, FGF-4, and GDF-5 in cavitation by defining expression patterns, immunohistochemically, during joint formation and establishing whether these are modified by in ovo immobilisation. We found that FGF-2 exhibited low level nuclear expression in chondrocytes and fibrocartilage cells close to presumptive joints, but showed significantly higher expression levels in cells at, and directly bordering, the forming joint cavity. This high-level joint line FGF-2 expression was selectively diminished in immobilised limbs. In contrast, we show that FGF-4 does not exhibit differential joint-line expression and was unaffected by immobilisation. GDF-5 protein also failed to show joint-line selective labelling, and although immobilisation induced a cartilaginous fusion across presumptive joints, it did not affect cellular GDF-5 expression patterns. Examining changes in GDF-5 expression in response to a direct mechanical strain stimulus in primary embryonic chick articular surface (AS) cells in vitro discloses only small mechanically-induced reductions in GDF-5 expression, suggesting that GDF-5 does not exert a direct positive contribution to the mechano-dependent joint cavitation process. This notion was supported by retroviral overexpression of UDPGD, a characteristic factor involved in hyaluronan (HA) accumulation at presumptive joint lines, which was also found to produce small decreases in AS cell GDF-5 expression. These findings support a direct mechano-dependent role for FGF-2, but not FGF-4, in the cavitation process and indicate that GDF-5 is likely to influence chondrogenesis positively without contributing directly to joint cavity formation. Developmental Dynamics 235:826,834, 2006. © 2006 Wiley-Liss, Inc. [source] Micropore modification in InPPHYSICA STATUS SOLIDI (A) APPLICATIONS AND MATERIALS SCIENCE, Issue 11 2008D. Nohavica Abstract The structural features and optical properties of microporous InP substrates used for epitaxial overgrowth of thin films have been investigated. Both crystalographically oriented (CO) and current line oriented (CLO) pore networks were created by electrochemical dissolution. Heat treatment of the InP pores converted them into microcavities maintaining the same crystallographic direction. The effect of phosphorus vapour pressure was proved to be crucial for the microcavity formation, since it influences the mass transport during heat treatment. Electron microscopy and photoluminescence experiments revealed the absence of significant extended defects, both after the pore and cavities formation. The capability of improved structural quality homo- and hetero-epitaxial overgrown films on the porous InP, was also demonstrated. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Differential regulation of GDF-5 and FGF-2/4 by immobilisation in ovo exposes distinct roles in joint formation,DEVELOPMENTAL DYNAMICS, Issue 3 2006E. Kavanagh Abstract Members of the fibroblast growth factor (FGF) family and growth and differentiation factor 5 (GDF-5) have been implicated in joint specification, but their roles in subsequent cavity formation are not defined. Cavity formation (cavitation) depends upon limb movement in embryonic chicks and factors involved in joint formation are often identified by their expression at the joint-line. We have sought support for the roles of FGF-2, FGF-4, and GDF-5 in cavitation by defining expression patterns, immunohistochemically, during joint formation and establishing whether these are modified by in ovo immobilisation. We found that FGF-2 exhibited low level nuclear expression in chondrocytes and fibrocartilage cells close to presumptive joints, but showed significantly higher expression levels in cells at, and directly bordering, the forming joint cavity. This high-level joint line FGF-2 expression was selectively diminished in immobilised limbs. In contrast, we show that FGF-4 does not exhibit differential joint-line expression and was unaffected by immobilisation. GDF-5 protein also failed to show joint-line selective labelling, and although immobilisation induced a cartilaginous fusion across presumptive joints, it did not affect cellular GDF-5 expression patterns. Examining changes in GDF-5 expression in response to a direct mechanical strain stimulus in primary embryonic chick articular surface (AS) cells in vitro discloses only small mechanically-induced reductions in GDF-5 expression, suggesting that GDF-5 does not exert a direct positive contribution to the mechano-dependent joint cavitation process. This notion was supported by retroviral overexpression of UDPGD, a characteristic factor involved in hyaluronan (HA) accumulation at presumptive joint lines, which was also found to produce small decreases in AS cell GDF-5 expression. These findings support a direct mechano-dependent role for FGF-2, but not FGF-4, in the cavitation process and indicate that GDF-5 is likely to influence chondrogenesis positively without contributing directly to joint cavity formation. Developmental Dynamics 235:826,834, 2006. © 2006 Wiley-Liss, Inc. [source] Comparative gene expression profiling of olfactory ensheathing glia and Schwann cells indicates distinct tissue repair characteristics of olfactory ensheathing gliaGLIA, Issue 12 2008Elske H.P. Franssen Abstract Olfactory ensheathing glia (OEG) are a specialized type of glia that support the growth of primary olfactory axons from the neuroepithelium in the nasal cavity to the brain. Transplantation of OEG in the injured spinal cord promotes sprouting of injured axons and results in reduced cavity formation, enhanced axonal and tissue sparing, remyelination, and angiogenesis. Gene expression analysis may help to identify the molecular mechanisms underlying the ability of OEG to recreate an environment that supports regeneration in the central nervous system. Here, we compared the transcriptome of cultured OEG (cOEG) with the transcriptomes of cultured Schwann cells (cSCs) and of OEG directly obtained from their natural environment (nOEG), the olfactory nerve layer of adult rats. Functional data mining by Gene Ontology (GO)-analysis revealed a number of overrepresented GO-classes associated with tissue repair. These classes include "response to wounding," "blood vessel development," "cell adhesion," and GO-classes related to the extracellular matrix and were overrepresented in the set of differentially expressed genes between both comparisons. The current screening approach combined with GO-analysis has identified distinct molecular properties of OEG that may underlie their efficacy and interaction with host tissue after implantation in the injured spinal cord. These observations can form the basis for studies on the function of novel target molecules for therapeutic intervention after neurotrauma. © 2008 Wiley-Liss, Inc. [source] Direct and indirect manipulation of the MEK-ERK pathway regulates the formation of a pericellular HA-dependent matrix by chick articular surface cells without modifying CD44 expresssionINTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, Issue 4 2004Edward R. Bastow Introduction Recent evidence suggests that hyaluronan (HA) facilitates the mechano-dependent joint cavity-forming process through the elaboration and retention of a HA-rich pericellular matrix in the developing joint interzone (IZ). The presumptive joint IZ phenotype shows a capacity to bind and synthesize HA and also exhibits elevated activated ERK, prior to synovial joint cavity formation (Lamb et al. 2001; Edwards et al. 1994; Dowthwaite et al. 1998). We have found that immobilization, which induces embryonic joint fusion with loss of the joint IZ phenotype, also reduces ERK activity levels in the IZ. As the signalling events regulating the synthesis and binding of HA have yet to be determined, we hypothesize that ERK activation plays a pivotal role in determining the presumptive joint IZ phenotype through HA synthetic and binding capacity. Materials and methods Chick articular surface (AS) cells were harvested from proximal tibiotarsal joints of embryos by collagenase digestion. Pericellular coat formation was assessed using the erythrocyte exclusion assay and cell-coat area ratios determined. ERK activity was modulated by transient transfection of GFP constructs of constitutively active (CA-) or dominant negative (DN-) forms of MEK, the direct upstream regulator of ERK or by treatment with the MEK inhibitor PD98059 (50 µm). ERK activation was monitored by immunochemistry. CD44 expression and ERK activation in PD98059-treated cells were monitored by immunoblotting and medium HA concentrations by ELISA. Results AS cells form large pericellular coats that are lost following hyaluronidase treatment and thus dependent upon HA for their construction. Treatment with PD98059 significantly reduced pericellular coat formation after 6 h. In parallel, we confirmed that PD98059 diminished active ERK expression without modifying overall levels of ERK, suggesting that the elaboration of large HA-pericellular coats is dependent upon MEK's activation of ERK. Western blot analysis of PD98059-treated cells showed that loss of pericellular coats was not, however, associated with any decreased levels of the cell surface HA receptor CD44. Although treatment with PD98059 did not change medium HA concentration after short times of exposure, at times (up to 6 h) during which coat loss was evident, prolonged treatment over 24 h significantly decreased medium HA concentration. Consistent with a role for ERK in pericellular coat formation, transfection with DN-MEK diminished, while CA-MEK increased, both active ERK expression and coat formation efficiency. We also found that, commensurate with this modification in coat forming efficiency, cells expressing DN-MEK exhibited a significant reduction in labelling of free HA on the cell surface. Discussion These studies extend our recent work to indicate that: (i) direct modulation of ERK activation by transfection with its endogenous upstream regulator modifies cell surface-associated HA (ii) PD98059-induced blockade of ERK activation restricts medium HA release and (iii) ERK-mediated changes in pericellular coat elaboration are independent of changes in cellular CD44 expression. These findings suggest an intimate relationship between ERK activation and the formation/retention of HA-rich pericellular matrices in vitro and highlight the role for ERK activation in regulating joint line-related differentiation. [source] Defining boundaries during joint cavity formation: going out on a limbINTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, Issue 2 2003K. J. Lamb Summary., Whilst factors controlling the site at which joints form within the developing limb are recognised, the mechanisms by which articular element separation occurs during the formation of the joint cavity have not been determined. Herein, we review the relationships between early limb patterning, embryonic movement, extracellular matrix composition, local signalling events and the process of joint cavity formation. We speculate that a pivotal event in this process involves the demarcation of signalling boundaries, established by local mechano-dependent modifications in glycosaminoglycan synthesis. In our opinion, studies that examine early patterning and also focus on local developmental alterations in tissue architecture are required in order to help elucidate the fundamental principals regulating joint formation. [source] Observations and interpretations at Vredefort, Sudbury, and Chicxulub: Towards an empirical model of terrestrial impact basin formationMETEORITICS & PLANETARY SCIENCE, Issue 5 2008Richard A. F. GRIEVE Assuming that the structures originally had the same morphology, the observations/interpretations for each structure are compared and extended to the other structures. This does not result in any major inconsistencies but requires that the observations be scaled spatially. In the case of Vredefort and Sudbury, this is accomplished by scaling the outer limit of particular shock metamorphic features. In the case of Chicxulub, scaling requires a reasoned assumption as to the formation mechanism of an interior peak ring. The observations/interpretations are then used to construct an integrated, empirical kinematic model for a terrestrial peak-ring basin. The major attributes of the model include: a set of outward-directed thrusts in the parautochthonous rocks of the outermost environs of the crater floor, some of which are pre-existing structures that have been reactivated during transient cavity formation; inward-directed motions along the same outermost structures and along a set of structures, at intermediate radial distances, during transient cavity collapse; structural uplift in the center followed by a final set of radially outward-directed thrusts at the outer edges of the structural uplift, during uplift collapse. The rock displacements on the intermediate, inward and innermost, outward sets of structures are consistent with the assumption that a peak ring will result from the convergence of the collapse of the transient cavity rim area and the collapse of the structural uplift. [source] Origin and emplacement of the impact formations at Chicxulub, Mexico, as revealed by the ICDP deep drilling at Yaxcopoil-1 and by numerical modelingMETEORITICS & PLANETARY SCIENCE, Issue 7 2004Dieter Stöffler We present and interpret results of petrographic, mineralogical, and chemical analyses of the 1511 m deep ICDP Yaxcopoil-1 (Yax-1) drill core, with special emphasis on the impactite units. Using numerical model calculations of the formation, excavation, and dynamic modification of the Chicxulub crater, constrained by laboratory data, a model of the origin and emplacement of the impact formations of Yax-1 and of the impact structure as a whole is derived. The lower part of Yax-1 is formed by displaced Cretaceous target rocks (610 m thick), while the upper part comprises six suevite-type allochthonous breccia units (100 m thick). From the texture and composition of these lithological units and from numerical model calculations, we were able to link the seven distinct impact-induced units of Yax-1 to the corresponding successive phases of the crater formation and modification, which are as follows: 1) transient cavity formation including displacement and deposition of Cretaceous "megablocks;" 2) ground surging and mixing of impact melt and lithic clasts at the base of the ejecta curtain and deposition of the lower suevite right after the formation of the transient cavity; 3) deposition of a thin veneer of melt on top of the lower suevite and lateral transport and brecciation of this melt toward the end of the collapse of the transient cavity (brecciated impact melt rock); 4) collapse of the ejecta plume and deposition of fall-back material from the lower part of the ejecta plume to form the middle suevite near the end of the dynamic crater modification; 5) continued collapse of the ejecta plume and deposition of the upper suevite; 6) late phase of the collapse and deposition of the lower sorted suevite after interaction with the inward flowing atmosphere; 7) final phase of fall-back from the highest part of the ejecta plume and settling of melt and solid particles through the reestablished atmosphere to form the upper sorted suevite; and 8) return of the ocean into the crater after some time and minor reworking of the uppermost suevite under aquatic conditions. Our results are compatible with: a) 180 km and 100 km for the diameters of the final crater and the transient cavity of Chicxulub, respectively, as previously proposed by several authors, and b) the interpretation of Chicxulub as a peak-ring impact basin that is at the transition to a multi-ring basin. [source] Neuromyelitis optica/Devic's disease: Gene expression profiling of brain lesionsNEUROPATHOLOGY, Issue 6 2008Jun-ichi Satoh Neuromyelitis optica (NMO), also known as Devic's disease, is an inflammatory demyelinating disease that affects selectively the optic nerves and the spinal cord, possibly mediated by an immune mechanism distinct from that of multiple sclerosis (MS). Recent studies indicate that NMO also involves the brain. Here, we studied gene expression profile of brain lesions of a patient with NMO by using DNA microarray, along with gene expression profile of the brains of Parkinson disease and amyotrophic lateral sclerosis patients. We identified more than 200 genes up-regulated in NMO brain lesions. The top 20 genes were composed of the molecules closely associated with immune regulation, among which marked up-regulation of interferon gamma-inducible protein 30 (IFI30), CD163, and secreted phosphoprotein 1 (SPP1, osteopontin) was validated by real time RT-PCR, Northern blot and Western blot analysis. Pathologically, CD68+ macrophages and microglia expressed intense immunoreactivities for IFI30 and CD163 in NMO lesions, consisting of inflammatory demyelination, axonal loss, necrosis, cavity formation, and vascular fibrosis. KeyMolnet, a bioinformatics tool for analyzing molecular interaction on the curated knowledge database, suggested that the molecular network of up-regulated genes in NMO brain lesions involves transcriptional regulation by the nuclear factor-kappaB (NF-,B) and B-lymphocyte-induced maturation protein-1 (Blimp-1). These results suggest that profound activation of the macrophage-mediated proinflammatory immune mechanism plays a pivotal role in development of NMO brain lesions. [source] Optical cavity formation on GaN using a conventional RIE systemPHYSICA STATUS SOLIDI (C) - CURRENT TOPICS IN SOLID STATE PHYSICS, Issue 6 2006F. G. Kalaitzakis Abstract We report on the fabrication of mirror-like facets on GaN-based laser structures, using a conventional RIE system and photoresist AZ 5214 as the etch mask. The roughness of the etched sidewalls is below 100 nm and the slope is practically vertical. For enhanced material protection during plasma etching, we describe an alternative lithography procedure with a double photoresist layer. (© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Effects of a nonsteroidal aromatase inhibitor on gonadal differentiation of bluegill sunfish Lepomis macrochirusAQUACULTURE RESEARCH, Issue 9 2010Ze-Xia Gao Abstract In the present study, the efficacy of Letrozole, a potent nonsteroidal aromatase inhibitor (AI), on gonadal sex differentiation and sex reversal was examined in bluegill sunfish (Lepomis macrochirus). In Experiment 1, using AI diet treatments (50, 150, 250 and 500 mg kg,1) from 30 to 90 days posthatch (dph), AI interrupted ovarian cavity formation at a dose of 500 mg kg1 diet and one intersex fish was identified in this group. The proportions of males in all the treated groups were significantly higher than those in the control group. In Experiment 2, using AI immersion treatments (250, 500 and 1000 ,g L,1) during 30,50 dph, the treated groups of 500 and 1000 ,g L,1 produced significantly more males than the control and 250 ,g L,1 groups. Histological examination revealed no differences in ovary or testis tissue between control and AI-treated fish. There were no significant differences detected in body weight and length among the AI treated and control groups (P>0.05) for both experiments. The results from these two experiments suggest that inhibition of aromatase activity by AI could influence sex differentiation in bluegill sunfish. [source] Urea interactions with protein groups: A volumetric study,BIOPOLYMERS, Issue 10 2010Soyoung Lee Abstract We determined the partial molar volumes and adiabatic compressibilities of N -acetyl amino acid amides, N -acetyl amino acid methylamides, N -acetyl amino acids, and short oligoglycines as a function of urea concentration. We analyze these data within the framework of a statistical thermodynamic formalism to determine the association constants for the reaction in which urea binds to the glycyl unit and each of the naturally occurring amino acid side chains replacing two waters of hydration. Our determined association constants, k, range from 0.04 to 0.39M. We derive a general equation that links k with changes in free energy, ,Gtr, accompanying the transfer of functional groups from water to urea. In this equation, ,Gtr is the sum of a change in the free energy of cavity formation, ,,GC, and the differential free energy of solute,solvent interactions, ,,GI, in urea and water. The observed range of affinity coefficients, k, corresponds to the values of ,,GI ranging from highly favorable to slightly unfavorable. Taken together, our data support a direct interaction model in which urea denatures a protein by concerted action via favorable interactions with a wide range of protein groups. Our derived equation linking k to ,Gtr suggests that ,,GI and, hence, the net transfer free energy, ,Gtr, are both strongly influenced by the concentration of a solute used in the experiment. We emphasize the need to exercise caution when two solutes differing in solubility are compared to determine the ,Gtr contribution of a particular functional group. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 866,879, 2010. [source] | ||||||||||||||||