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Selected AbstractsMovement and behaviour of large southern bluefin tuna (Thunnus maccoyii) in the Australian region determined using pop-up satellite archival tagsFISHERIES OCEANOGRAPHY, Issue 5 2008TOBY A. PATTERSON Abstract Pop-up satellite archival tags (PSATs) were deployed on 52 large (156,200 cm length to caudal fork) southern bluefin tuna (Thunnus maccoyii) in the western Tasman Sea during the austral winters of 2001,2005. Southern bluefin tuna (SBT) were resident in the Tasman Sea for up to 6 months with movements away from the tagging area occurring at highly variable rates. The data indicated a general tendency for SBT to move south from the tagging area in the Western Tasman Sea. Four individuals migrated west along the southern continental margin of Australia and into the Indian Ocean. Three individuals moved east into the central Tasman Sea, with one individual reaching New Zealand. We also describe the first observed migration of an SBT from the Tasman Sea to the Indian Ocean spawning grounds south of Indonesia. Individuals spent most of their time relatively close to the Australian coast, with an estimated 84% of time spent in the Australian Fishing Zone. SBT favored temperatures between 19 and 21°C, adjusting their depth to the vertical temperature distribution. Distinct diurnal diving patterns were observed and adjustment of depth to maintain constant ambient light levels over a 24-h period. The findings of this study are a significant advance toward greater understanding of the spatial dynamics of large SBT and understanding the connectivity between distant regions of their distribution. [source] Anguillicoloides crassus infection of European eel, Anguilla anguilla (L.), in inland waters of Estonia: history of introduction, prevalence and intensityJOURNAL OF APPLIED ICHTHYOLOGY, Issue 2010A. Kangur Summary Eel fishery in Estonian inland waters depends entirely on the stocking of glass eels or pre-grown (farmed) eels. Via importation of live eels of 20,30 cm length the non-indigenous swimbladder nematode Anguillicoloides crassus was probably introduced via Germany into Lake Võrtsjärv in 1988, and has since spread to many inland waters of Estonia. In 1992, the parasite was found in eel caught from Lake Võrtsjärv. Between 1992 and 2002 and additionally in 2008, we examined in total 870 eels from Lake Võrtsjärv (270 km2) and in 2008, 63 eels from three small lakes for adult A. crassus. The aim of the study was to obtain information on the variation of A. crassus infection in eels in Estonian lakes, to determine the temporal dynamics of prevalence and intensity of infection, and to establish a relationship between the length of host and intensity of infection in the eels in Lake Võrtsjärv. There appeared to be a pronounced variation in prevalences of infected eels (from 3.7% to 100%) between the four investigated lakes. However, in Lake Võrtsjärv, the prevalence of adult A. crassus infection remained stable (mean about 65%) for many years. The average number of nematode per infected eel (mean intensity) ranged from 12.6 ± 2.5 in 1993 to 4.0 ± 0.6 in 1999 in Lake Võrtsjärv, while it was significantly higher (P < 0.0001) in the period 1992,1998 compared to 1999,2002 and 2008. The mean number of parasites per swimbladder was not related to eel length and no statistical difference was found in the condition factor of infected and non-infected eels. Although under normal environmental conditions A. crassus has not caused serious disease problems to eels in the study area, high intensity of parasite infection may contribute to eel kills due to oxygen deficiency in winter under the ice in Lake Võrtsjärv. [source] Continuous process for production of hydrogenated nitrile butadiene rubber using a Kenics® KMX static mixer reactorAICHE JOURNAL, Issue 11 2009Chandra Mouli R. Madhuranthakam Abstract A continuous process for hydrogenating nitrile butadiene rubber (NBR) was developed and its performance was experimentally investigated. A Kenics® KMX static mixer (SM) is used in the process as a gas,liquid reactor in which gaseous hydrogen reacts with NBR in an organic solution catalyzed by an organometallic complex such as an osmium complex catalyst. The Kenics® KMX SM was designed with 24 mixing elements with 3.81 cm diameter and arranged such that the angle between two neighboring elements is 90°. The internal structure of each element is open blade with the blades being convexly curved. The dimensions of the SM reactor are: 3.81 cm ID 80 S and 123 cm length and was operated cocurrently with vertical upflow. The NBR solutions of different concentrations (0.418 and 0.837 mol/L with respect to [CC]) were hydrogenated by using different concentrations of the osmium catalyst solution at various residence times. The reactions were conducted at a constant temperature of 138°C and at a constant pressure of 3.5 MPa. From the experimental results, it is observed that a conversion and/or degree of hydrogenation above 95% was achieved in a single pass from the designed continuous process. This is the first continuous process for HNBR production that gives conversions above 95% till date. Optimum catalyst concentration for a given mean residence time to achieve conversions above 95% were obtained. Finally, a mechanistic model for the SM reactor performance with respect to hydrogenation of NBR was proposed and validated with the obtained experimental results. © 2009 American Institute of Chemical Engineers AIChE J, 2009 [source] Micellar electrokinetic capillary chromatography of methylxanthines-containing beverages: discussion of the molecular species involvedJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 4 2005Alicia B Pomilio Abstract Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mg l,1, the limit of quantitation was 2.5 mg l,1 and the work range was 2.5,300 mg l,1. This HPCE,MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed. Copyright © 2004 Society of Chemical Industry [source] Potential mapping technique for the detection of corrosion in reinforced concrete structures: Investigation of parameters influencing the measurement and determination of the reliability of the methodMATERIALS AND CORROSION/WERKSTOFFE UND KORROSION, Issue 2 2009Y. Schiegg Abstract The potential distribution around a corrosion site in concrete was modeled by means of a simple mathematical model for a rod-shaped anode. Based on these calculations the influencing parameters and the limits of the potential mapping technique were determined. They demonstrate that the location of anodes depends on the anode length, the concrete coverage, and the measuring grid. Moreover small anodes with less than 1 cm length are virtually impossible to localize. Investigations on concrete elements without reinforcing steel showed that heterogeneous water content and chloride distribution can result in potential differences of up to 150 mV on the concrete surface. This effect was related to the streaming potential and the diffusion overpotential in the concrete. In order to determine the reliability of the potential mapping technique in the field application, the concrete on a tunnel wall was removed after the measurement of the potential distribution and the corrosion loss of the steel was determined. It was found that all corrosion sites with more than 2 mm material loss were detected by the potential mapping technique. Only some smaller corrosion sites were not found. Based on this investigation, the applicability of the potential mapping technique for detecting corrosion sites was confirmed. The observed high reliability of the potential mapping technique can be explained by a combination of the heterogeneous wetting of the concrete, the inhomogeneous contamination with chloride, and the macrocell formation. [source] Foreign body granuloma in the submental region due to fish bone: a case reportORAL SURGERY, Issue 1-2 2010X. Ding Abstract The purpose of this article was to describe the clinical and microscopic features of a foreign-body granuloma in submental region that resulted from a fish bone embedded in the floor of mouth. A 45-year-old female patient complained of a hard mass in submental region. Clinical examination showed a non-compressible, firm, fixed lump, painless on palpation. Ultrasound examination showed an internally uneven, uncircumscribed, hypoechoic mass with a steaky hyperechoic spot of 1.1 cm length in the center. A microscopic examination showed newly-formed granuloma, composed of lympocytes and epithelioid cells, and some microabscess with neurophiles. The final diagnosis was a foreign-body granuloma with fish bone. Even though foreign-body granulomas in submental region are rare lesions, surgeon should be familiar with their features and include them in the differential diagnosis of tissue masses. [source] Modelling approaches to compare sorption and degradation of metsulfuron-methyl in laboratory micro-lysimeter and batch experimentsPEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 12 2003Maik Heistermann Abstract Results of laboratory batch studies often differ from those of outdoor lysimeter or field plot experiments,with respect to degradation as well as sorption. Laboratory micro-lysimeters are a useful device for closing the gap between laboratory and field by both including relevant transport processes in undisturbed soil columns and allowing controlled boundary conditions. In this study, sorption and degradation of the herbicide metsulfuron-methyl in a loamy silt soil were investigated by applying inverse modelling techniques to data sets from different experimental approaches under laboratory conditions at a temperature of 10 °C: first, batch-degradation studies and, second, column experiments with undisturbed soil cores (28 cm length × 21 cm diameter). The column experiments included leachate and soil profile analysis at two different run times. A sequential extraction method was applied in both study parts in order to determine different binding states of the test item within the soil. Data were modelled using ModelMaker and Hydrus-1D/2D. Metsulfuron-methyl half-life in the batch-experiments (t1/2 = 66 days) was shown to be about four times higher than in the micro-lysimeter studies (t1/2 about 17 days). Kinetic sorption was found to be a significant process both in batch and column experiments. Applying the one-rate-two-site kinetic sorption model to the sequential extraction data, it was possible to associate the stronger bonded fraction of metsulfuron-methyl with its kinetically sorbed fraction in the model. Although the columns exhibited strong significance of multi-domain flow (soil heterogeneity), the comparison between bromide and metsulfuron-methyl leaching and profile data showed clear evidence for kinetic sorption effects. The use of soil profile data had significant impact on parameter estimates concerning sorption and degradation. The simulated leaching of metsulfuron-methyl as it resulted from parameter estimation was shown to decrease when soil profile data were considered in the parameter estimation procedure. Moreover, it was shown that the significance of kinetic sorption can only be demonstrated by the additional use of soil profile data in parameter estimation. Thus, the exclusive use of efflux data from leaching experiments at any scale can lead to fundamental misunderstandings of the underlying processes. Copyright © 2003 Society of Chemical Industry [source] Vertical Migration and Motility Responses in Three Marine Phytoplankton Species Exposed to Solar Radiation,PHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 4 2007Peter R. Richter ABSTRACT Diurnal vertical migration in the water column and the impact of solar radiation on motility were investigated in three marine phytoplankton species: Tetraselmis suecica, Dunaliella salina and Gymnodinium chlorophorum. Cells were exposed to solar radiation either in ultraviolet radiation (UVR, 280,400 nm) transparent Plexiglas tubes (45 cm length, 10 cm diameter) or in quartz tubes under three radiation treatments: PAB (280,700 nm), PA (320,700 nm) and P (400,700 nm). The three species displayed different behavior after exposure to solar radiation. Tetraselmis suecica was insensitive to UVR and under high solar radiation levels, cells accumulated preferentially near the surface. Exposure experiments did not indicate any significant changes in swimming speed nor in the percentage of motile cells after 5 h of exposure. On the other hand, D. salina was sensitive to UV-B displaying a significant decrease in swimming speed and percentage of motile cells after 2,3 h of exposure. Moreover, D. salina cells migrated deep in the water column when irradiance was high. The response of G. chlorophorum was in between that of the other two species tested, with a slight (but significant) decrease in swimming speed and percentage of motile cells in all radiation treatments after 5 h of exposure. While G. chlorophorum cells were more or less homogenously distributed in the water column, a slight (but significant) avoidance response to high radiation was observed at local noon, with cells migrating deep in the water column. Our data clearly indicate that these sub-lethal effects of solar radiation are species-specific and they might have important implications for the aquatic ecosystem. [source] Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysisPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 7 2009Dingyin Tao Abstract Microcolumn RPLC (,RPLC) is one of the optimum separation modes for shotgun proteomic analysis. To identify as many proteins as possible by MS/MS, the improvement on separation efficiency and peak capacity of ,RPLC is indispensable. Although the increase in column length is one of the effective solutions, the preparation of a long microcolumn is rather difficult due to the high backpressure generated during the packing procedure. In our recent work, through connecting microcolumns of 5, 10, and 15,cm length via unions with minimal dead volume, long microcolumns with length up to 30,cm were obtained, with which 318 proteins were identified from proteins extracted from Escherichia coli by ,RPLC-ESI MS/MS, and similar distributions of Mw and pI were found with single and various coupled microcolumns. Furthermore, by using MS/MS with improved sensitivity, with such a serially coupled 30,cm long microcolumn, 1692 proteins were identified within 7,h from rat brain tissue, with false positive rate (FPR) <1%. All these results demonstrated that serially couple microcolumns might be of great promising to improve the separation capacity of ,RPLC in shotgun proteomic analysis. [source] Transfer of toxaphene and chlordane into farmed rainbow trout, Oncorhynchus mykiss (Walbaum) via feedAQUACULTURE RESEARCH, Issue 12 2002H Karl Abstract The study was carried out to quantitate the transfer of toxaphene and chlordane compounds from commercial fish feed into the edible part of rainbow trout, Oncorhynchus mykiss (Walbaum) under normal rearing conditions. Trout were fed with unspiked high energy feed for salmon (fat content 26,30%) over a period of 19 months. The average weight of trout increased from 10 g to more than 2092 g, reaching sizes of 51 cm length. Considerable amounts of toxaphene and chlordane residues were transferred from fish feed into trout muscle. Toxaphene concentrations increased up to 8.6 µg (, toxaphene indicator compounds 1,3) kg,1 wet weight (w.w.) and chlordane reached 5.3 µg ,oxy -, trans -, cis -chlordane + t -nonachlor kg,1 w.w. Results are also discussed on the contaminant levels based on the fat content and the effect of sexual maturation is considered in this study. The data allow the establishment of transfer rates for toxaphene and chlordane congeners from high energy diet into the edible part of farmed rainbow trout. [source] Enantioselective analysis of primaquine and its impurity quinocide by capillary electrophoresisBIOMEDICAL CHROMATOGRAPHY, Issue 3 2009Abdalla A. Elbashir Abstract A capillary electrophoretic (CE) method for the baseline separation of the enantiomers of primaquine diphosphate (PQ) and quinocide (QC) (a major contaminant) in pharmaceutical formulations is proposed. Both components were separated under the following conditions: 50 mm tris phosphate buffer (pH 3.0) containing 15 mm hydroxypropyl- , -cyclodextrin (HP- , -CD) as background electrolyte; applied voltage, 16 kV; capillary temperature, 25°C; detection wavelength, 254 nm; hydrostatic injection, 10 s. The separations were conducted using a 35 cm length and 50 µm i.d. uncoated fused silica capillary column. Under the optimized conditions, the components were successfully separated in about 5 min. Intraday precision of migration time and corrected peak areas when expressed as relative standard deviation ranged from 0.17 to 0.45 and 2.60 to 3.94%, respectively, while the interday precision ranged from 2.59 to 4.20 and 3.15 to 4.21%, respectively. After the validation exercise, the proposed method was applied for the determination of QC impurity in PQ formulations. Copyright © 2008 John Wiley & Sons, Ltd. [source] Effects of feeding methods on eating frustration in stabled horsesANIMAL SCIENCE JOURNAL, Issue 5 2004Shigeru NINOMIYA ABSTRACT Five feeding methods were tested in stabled horses: (i) cutting hay (hay was cut into 5 cm lengths), (ii) delaying feeding time (feeding was delayed until 1 h after the usual time), (iii) increasing the feeding frequency (hay was divided into two portions; one half was given at the normal time and the other 1 h later), (iv) increasing the feeding locations (hay was available at three locations), and (v) increasing the hay varieties (three species of hay were used). The behavioral durations in the 2 h after the experimental feedings were compared with those after usual feeding. In the cutting hay treatment, hay-eating time decreased (P < 0.05), whereas bedding-eating time and resting time tended to increase. In the delaying feeding time treatment, bedding investigation time increased (P < 0.01) and hay-eating time tended to increase. In the increasing the feeding frequency treatment, hay-eating time tended to increase. In the increasing the feeding locations treatment, bedding investigation time tended to decrease. In the increasing the hay varieties treatment, resting time tended to decrease and hay-eating time tended to increase. It was suggested that the former two feeding methods might stimulate and the latter three feeding methods might suppress eating frustration in terms of increasing consummatory behavior (eating) and decreasing appetitive (investigating) and displacement behavior (resting). [source] |