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Cm Column (cm + column)
Selected AbstractsPurification and Characterization of Acid Phosphatase from the Egg of the Lady Beetle, Harmonia axyridis (Coccinellidae: Coleoptera)ENTOMOLOGICAL RESEARCH, Issue 1 2004Jun Hyuk LEE ABSTRACT Acid phosphatase (AP) in the egg of the lady beetle, Harmonia axyridis, was purified and characterized. Ammonium sulfate precipitation, CM column and isoelectrofocusing (IEF) were applied to purify an estimated molecular weight of 66 kDa AP. The purity was checked by SDS PAGE, native PAGE and Western blot. AP was detected in the hemolymph of the female and the egg, but not in the male on the blotting. Km of AP for a substrate, p -nitrophenyl phosphate (p -NPP), was 1.64 x 10 -4 M. AP had the optimum enzymatic activity at pH 3.5. In inhibition tests performed with various chemicals, ammonium molybdate suppressed 99% of the enzyme activity of AP even at the concentration of 5 x 10 -4 mM. AP was stable up to 50°C. [source] Evaluation of a hydrazide-linked ,1 -acid glycoprotein chiral stationary phase: Separation of R - and S -propranololJOURNAL OF SEPARATION SCIENCE, JSS, Issue 10 2006Hai Xuan Abstract The binding and chiral separation of R - and S -propranolol was investigated on a new type of ,1 -acid glycoprotein (AGP) column. This column was prepared through the controlled and mild oxidation of AGP, followed by the immobilization of this protein to hydrazide-activated silica. The effects of temperature, pH, ionic strength, and organic modifiers on the retention and separation of R - and S -propranolol were investigated on this column. Both the association equilibrium constants and number of binding sites for R/S -propranolol on the AGP column were found to increase with temperature and affect the measured retention factors for these compounds. Regarding the other factors, a change in the organic modifier concentration was found to give the largest change in retention and separation. It was found through these studies that both coulombic and hydrophobic interactions played important roles in determining the retention of R - and S -propranolol on the AGP column. The efficiency and separation impedance of this system were also considered. Under the final optimum conditions identified in this study, it was possible to separate R - and S -propranolol with a resolution of greater than 1.38 in less than 5 min on a 4.1 mm I.D.×5 cm column. [source] Studies on azaspiracid biotoxins.RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 24 2002In this study, the performance of monolithic columns was evaluated for ultrafast liquid chromatography/mass spectrometry (LC/MS) analyses and for high-resolution separations of several azaspiracid biotoxin analogs. Because of their high permeability, monolithic columns offer a number of advantages over conventional packed columns; viz., very low backpressures and relatively flat van Deemter curves at high flow rates. That is, very high flow rates can be used for ultrafast analyses or, by using longer than normal columns, high-resolution separations are possible. In a series of experiments, we varied the mobile phase flow rates between 1 and 8,mL/min, and studied their impact on chromatographic parameters such as retention time, resolution, number of plates and pressure. The chromatographic run times could be reduced to ca. 30,s without a significant change in the separation efficiency. A signal intensity comparison revealed interesting differences between atmospheric-pressure chemical ionization (APCI) and electrospray ionization (ESI) in their flow-rate dependency. An explanation with respect to the behavior as of a mass-flow or a concentration-dependent device is given in the paper. Additionally, the column length was varied between 10 and 70,cm. As a result, the number of theoretical plates increased substantially. In the example shown in the report, an increase from 13,000 plates for a 10-cm column to 80,000 for a 70-cm column is demonstrated. In addition, the potential of the monolithic columns for high-resolution LC/MS separations is shown for a complex biotoxin mixture, which was separated on a 40-cm-long column. Copyright © 2002 John Wiley & Sons, Ltd. [source] Leaching of heavy metals and nutrients from calcareous sandy-loam soil receiving municipal solid sewage sludgeJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 3 2010Mohsen Jalali Abstract Leaching column experiments were conducted to determine the degree of mobility of heavy metals (HMs) and nutrients after the addition of municipal solid sewage sludge (MSS) in a sandy-loam soil. Treatments were (1) soil application of low metal content MSS, (2) soil application of metal-enriched municipal solid sewage sludge (EMSS), and (3) control. The MSS application represented a dose of 200 Mg dry weight (dw) ha,1. Soil columns were incubated at room temperature for 15 d and were irrigated daily with distilled water to make a total of 557,mm. Leachates were collected and analyzed for HMs and nutrients. The Ni and Pb added to soil via MSS and EMSS were found to be leached through the 20,cm columns of calcareous sandy soil although Ni and Pb concentrations in the percolate were small relative to the total amounts of metals applied. Losses of K+ from the EMSS, MSS, and control were 92.5, 82.0, and 52.5,kg ha,1, respectively. Losses of Mg2+ were in the range from 104.4 (control treatment) to 295.2,kg ha,1 (EMSS), while the loss of Ca2+ was in the range from 265.0 (control treatment) to 568.2,kg ha,1 (EMSS). The results showed that the amounts of P leached from EMSS (3.02,kg ha,1) and MSS (2.97,kg,1 ha,1) were significantly larger than those from the control treatment (1.54,kg ha,1). The geochemical code Visual MINTEQ was used to calculate saturation indices. Leaching of P in different treatments was controlled by rate-limited dissolution of hydroxyapatite, ,-tri-Ca phosphate, and octa-Ca phosphate. The results indicate that application of MSS to a sandy soil, at the loading rate used in this study, may pose a risk in terms of groundwater contamination with Ni, Pb, and the studied nutrients. [source] | ||||||||||