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Buffalo
Kinds of Buffalo Terms modified by Buffalo Selected AbstractsDental trauma that require fixation in a children's hospitalDENTAL TRAUMATOLOGY, Issue 1 2008Timothy Bruns Complex injuries to permanent teeth and their periodontium require immediate repositioning and stabilization. Many of these emergencies are treated by pediatric dental residents at the Women and Children's Hospital of Buffalo, Buffalo, New York. The purpose of this study was to characterize these complex injuries of permanent teeth that require emergency treatment in a Children's Hospital. All of the cases of dental trauma which had involved permanent teeth and which had been treated with a splint in 2001 and 2002 were reviewed. There were 79 patients that were between 5 and 19 years of age with twice as many males (54) as females (25). The number of males increased from childhood (5,10 years) to early adolescence (11,15 years) and then decreased rapidly in late adolescence (16,19 years), whereas the number of females decreased steadily with age. Most of the incidents occurred during the summer months (72%), particularly in June and July (42%), and Fridays and Saturdays were the busiest days of the week. Most of the injuries were caused by organized and recreational sporting activities (39%) and accidental falls (33%), followed by interpersonal violence (15%) and a few motor vehicle accidents (7%). The 173 permanent tooth injuries were mostly luxations (62%) or avulsions (20%), with only a few fractures of the alveolar bone (5%) or tooth root (1%). Most of the displacements were lateral luxations (40%) or extrusions (18%) with only a few intrusions (3%). These injuries most commonly afflicted the maxillary central incisors (54%), followed by the maxillary laterals (18%) and mandibular centrals (17%). The emergency treatment that was provided at the Children's Hospital included replantation and repositioning, and the placement of a semi-rigid or flexible splint. [source] Local Indicators of Network-Constrained Clusters in Spatial Point PatternsGEOGRAPHICAL ANALYSIS, Issue 3 2007Ikuho Yamada The detection of clustering in a spatial phenomenon of interest is an important issue in spatial pattern analysis. While traditional methods mostly rely on the planar space assumption, many spatial phenomena defy the logic of this assumption. For instance, certain spatial phenomena related to human activities are inherently constrained by a transportation network because of our strong dependence on the transportation system. This article thus introduces an exploratory spatial data analysis method named local indicators of network-constrained clusters (LINCS), for detecting local-scale clustering in a spatial phenomenon that is constrained by a network space. The LINCS method presented here applies to a set of point events distributed over the network space. It is based on the network K -function, which is designed to determine whether an event distribution has a significant clustering tendency with respect to the network space. First, an incremental K -function is developed so as to identify cluster size more explicitly than the original K -function does. Second, to enable identification of cluster locations, a local K -function is derived by decomposing and modifying the original network K -function. The local K -function LINCS, which is referred to as KLINCS, is tested on the distribution of 1997 highway vehicle crashes in the Buffalo, NY area. Also discussed is an adjustment of the KLINCS method for the nonuniformity of the population at risk over the network. As traffic volume can be seen as a surrogate of the population exposed to a risk of vehicle crashes, the spatial distribution of vehicle crashes is examined in relation to that of traffic volumes on the network. The results of the KLINCS analysis are validated through a comparison with priority investigation locations (PILs) designated by the New York State Department of Transportation. [source] Buffalo (Bubalus bubalis) interleukin-2: sequence analysis reveals high nucleotide and amino acid identity with interleukin-2 of cattle and other ruminantsINTERNATIONAL JOURNAL OF IMMUNOGENETICS, Issue 4 2002E. Sreekumar Summary A 4400-bp genomic sequence and a 332-bp truncated cDNA sequence of the interleukin-2 (IL-2) gene of Indian water buffalo (Bubalus bubalis) were amplified by polymerase chain reaction and cloned. The coding sequence of the buffalo IL-2 gene was assembled from the 5, end of the genomic clone and the truncated cDNA clone. This sequence had 98.5% nucleotide identity and 98% amino acid identity with cattle IL-2. Three amino acid substitutions were observed at positions 63, 124 and 135. Comparison of the predicted protein structure of buffalo IL-2 with that of human and cattle IL-2 did not reveal significant differences. The putative amino acids responsible for IL-2 receptor binding were conserved in buffalo, cattle and human IL-2. The amino acid sequence of buffalo IL-2 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. [source] The impact of elephants, Loxodonta africana, on woody vegetation through selective debarking in Samburu and Buffalo Springs National Reserves, KenyaAFRICAN JOURNAL OF ECOLOGY, Issue 1 2010Festus W. Ihwagi Abstract The impact of elephants on the woody plant community through debarking was investigated in Samburu and Buffalo Springs National Reserves, Kenya. Acacia elatior Brenan, the most abundant tree species in the riverine zone, accounted for 68% (n = 1375) of woody plants. A. tortilis (Forsskal) Hayne dominated plots away from the river. Debarking incidences were significantly higher for A. elatior than for other species indicating selective utilization. The riverine zone by virtue of having more trees of the preferred species, A. elatior, had the highest debarking incidences. Presence of very few saplings along the river is attributed to both elephant trampling and herbivory by other species. An estimated 38.5% and 22.5% of the riverine A. elatior and A. tortilis trees respectively, were bound to die within the next 4,5 years because of severe debarking, ,75% of bark circumference. Debarking was positively correlated with stem circumference; the medium-sized trees being the worst affected by the elephants' selective debarking behaviour. Intense debarking incidences were recorded during the dry season. Through the elephants' selective debarking, the riverine habitat is bound to open up gradually, leading to considerable habitat change in the near future. Elephant impact on vegetation is less away from the river and increases with their densities. Résumé L'impact des éléphants sur la communauté des plantes ligneuses, à cause de l'enlèvement des écorces, a étéétudié dans les Réserves Nationales de Samburu et de Buffalo Springs, au Kenya. L'Acacia elatior Brenan, le plus abondant de la zone riveraine, comptait pour 68% (n = 1375) des plantes ligneuses. A. tortilis (Forsskal) Haine dominait les parcelles plus éloignées de la rivière. Les cas d'écorçage étaient significativement plus nombreux pour A. elatior que pour les autres espèces, ce qui indique une utilisation sélective. La zone riveraine, du fait qu'elle compte plus d'arbres de l'espèce préférée, A. elatior, comptait aussi le plus grand nombre de cas d'écorçage. La présence de très peu de jeunes plants le long de la rivière est attribuée au piétinement par les éléphants et à la consommation par d'autres espèces herbivores. On estime que respectivement 38,5% et 22,5% des A. tortilis et A. elatior de la zone riveraine devraient mourir dans les 4,5 années à venir, en raison d'écorçages sévères qui peuvent être de ,75% de la circonférence de l'arbre. L'écorçage était positivement liéà la circonférence du tronc, les arbres de diamètre moyen étant les plus gravement affectés par le comportement sélectif des éléphants. Des cas d'écorçage intense ont été enregistrés au cours de la saison sèche. En raison de l'écorçage sélectif des éléphants, l'habitat riverain est condamnéà s'ouvrir progressivement, ce qui entraînera un changement considérable dans un proche avenir. L'impact des éléphants sur la végétation est moindre lorsqu'on s'éloigne de la rivière, et il augmente en fonction de leur densité. [source] 'You're So Fat!': Exploring Ojibwe DiscourseJOURNAL OF LINGUISTIC ANTHROPOLOGY, Issue 2 2001Linda C. Garro 'You're So Fat!': Exploring Ojibwe Discourse. Roger Spielmann. Toronto, Buffalo, and London: University of Toronto Press, 1998. xi. 280 pp. [source] RELATING PHYTOPLANKTON DYNAMICS AND PRODUCTION TO SEDIMENT RESUSPENSION IN SOUTHERN LAKE MICHIGANJOURNAL OF PHYCOLOGY, Issue 2001Article first published online: 24 SEP 200 Millie, D. F.1, Fahnenstiel, G. L.2, Carrick, H. J.3, Lohrenz, S. E.4, & Schofield, O. M. E.5 1USDA-Agricultural Research Service, Sarasota, FL 34236, USA, 2NOAA-Lake Michigan Field Station, Muskegon, MI 49441, USA, 3Institute of Marine Science, University of Southern Mississippi, Stennis Space Center, MS 39529, USA, 4Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14060, USA; 5Isttitute of Marine and Coastal Sciences, Rutgers University, 71 Dudley Road New Brunswick NJ 08901 USA, Sediment resuspension is an annually recurrent feature during spring holomixis in southern Lake Michigan. Relationships between resuspension events and phyt-oplankton biomass, compositional dynamics, and pro-duction were evaluated during 1998 and 1999. Increased water-column light attenuation (KPAR) and suspended particulate matter (SPM) concentrations corresponded with resuspension events within nearshore regions. However, neither KPAR nor SPM corresponded with chlorophyll (Chl) a concentrations, indicating no impact of resuspension on instantaneous biomass accumulation. Diatoms and cryptophytes dominated phytoplankton assemblages and together typically comprised greater than 85% of the Chl a. The associations of SPM/KPAR with diatom Chl a, and the inverse relationship between relative diatom and crypto-phyte Chl a corresponded with the dominance of diatoms and cryptophytes in near- and offshore waters, respectively. Moreover, a spatial variation in species composition occurred during resuspension events; small, centric diatoms exhibiting meroplanktonic life histories and large, pennate diatoms considered benthic in origin were associated with sediment resuspension whereas large, net diatoms and cryptophytes typically comprising phytoplankton of the annual spring bloom and of optically-clear, offshore waters were not. The presence of viable diatom photopigments and the abundance of small centric diatoms within the surficial sediments, established this layer as the source of meroplankton. Integral production was dramatically reduced within sediment-impacted waters; however, nearshore assemblages appeared to have greater photosynthetic capacities than offshore assemblages. Although resuspension dramatically influenced near-shore phytoplankton assemblages, it appeared to have little, if any relationship with the compositional development of the annual spring bloom. [source] Reduced dispersal and opportunistic territory acquisition in male lions (Panthera leo)JOURNAL OF ZOOLOGY, Issue 2 2003Paul J. Funston Abstract Life-history patterns in lions Panthera leo living in savanna woodlands of the Kruger National Park, South Africa, were investigated and compared with those of the Tanzanian ,plains-like' ecosystems (e.g. Serengeti Plains and Ngorongoro Crater). First, lower levels of mortality in the juvenile age classes were found in Kruger lions, which in turn, extend the inter-birth period. A further difference was a prolonged period of association of sub-adult males with their natal pride, either directly or in a land tenure system that has not been described previously. Most (80%) of young male coalitions rather than becoming nomadic, remained close to their natal territory after leaving the pride, either as non-territorial sub-adults or adults and even as territorial adults. Only 20% of coalitions did not stay close to their natal range, one of which acquired a territory 20 km away from its natal pride. The pattern of territory acquisition, in fact, was one in which the majority of holders acquired territories close to their natal ranges. These behaviour patterns contrast markedly with those from ,plains-like' ecosystems where dispersing males usually move far away from their natal pride's range (>200) km and often remain nomadic for extended periods of time. Dense bush and access to sufficient prey resources in the form of resident buffalo Syncerus caffer herds may be important factors allowing extended residence near the natal pride's territory. Buffalo were more available in our study area habitat than in neighbouring habitats, and comprised the majority of male lion kills. Extended male residence contrasts markedly with current theory on dispersal in polygynous mammals, which holds that only one sex (females for lions) gain an advantage by staying close to the territory of their natal pride. In Kruger it seems that both sexes gain an advantage by not dispersing far, and use currently undocumented mechanisms to avoid inbreeding. [source] System Change through Collaboration,Eight Steps for Getting from There to HereJUVENILE AND FAMILY COURT JOURNAL, Issue 4 2002JUDGE SHARON S. TOWNSEND ABSTRACT Family courts and child welfare agencies across the country are charged with protecting the safety of our children. That mission has become more challenging with increasing federal legislation and decreasing funding. In Buffalo, N.Y., the Family Court and the Department of Social Services have teamed up to respond to this challenge. With minimal additional staffing and resources, they have led a collaboration of agencies and service providers to change the way business is done in child welfare. By engaging each other in an interagency system change effort, the amount of time children spend in foster care has been reduced. The collaboration has been able to accomplish in a relatively short time what no agency had previously been able to accomplish on its own. The beneficiaries have been the children and families of Erie County. [source] The connection: Schooling, youth development, and community building,The Futures Academy caseNEW DIRECTIONS FOR YOUTH DEVELOPMENT, Issue 122 2009Henry Louis Taylor Jr. Universities, because of their vast human and fiscal resources, can play the central role in assisting in the development of school-centered community development programs that make youth development their top priority. The Futures Academy, a K,8 public school in the Fruit Belt, an inner-city neighborhood in Buffalo, New York, offers a useful model of community development in partnership with the Center for Urban Studies at the State University of New York at Buffalo. The goal of the project is to create opportunities for students to apply the knowledge and skills they learn in the classroom to the goal of working with others to make the neighborhood a better place to live. The efforts seek to realize in practice the Dewey dictum that individuals learn best when they have "a real motive behind and a real outcome ahead." [source] Stepping Back, Stepping ForwardNURSING FOR WOMENS HEALTH, Issue 5 2001Woman's Health Care in Bosnia-Herzegovina Little has been written about the health care system of the former Yugoslavia. In 1996, the U.S. Agency for International Development (US AID) awarded a 2 million grant to Buffalo General Hospital and The Children's Hospital of Buffalo, two of the five hospitals that now form Kaleida Health in Buffalo, NY, to provide training, education and supplies to the Tuzla Clinical Center in Bosnia. The funds were targeted at [source] Abstracts of the 27th Annual Meeting of the Human Biology Association, Buffalo, New York April 12,14 2001AMERICAN JOURNAL OF HUMAN BIOLOGY, Issue 1 2002Article first published online: 4 JAN 200 First page of article [source] Effects of FSH and LH on Steroid Production by Buffalo (Bubalus bubalis) Granulosa Cells Cultured In Vitro Under Serum-Free ConditionsREPRODUCTION IN DOMESTIC ANIMALS, Issue 5 2010M Shanmugam Contents The objective of this study was to examine the effects of FSH and LH on oestradiol-17, and progesterone production by buffalo granulosa cells cultured under serum-free conditions. Granulosa cells (3 × 105) from small (,5 mm diameter) follicles were cultured for up to 4 days in 48-well plates coated with 3.3 ,g/cm2 fibronectin in Dulbecco's modified Eagle's medium (DMEM) : nutrient mixture F-12 Ham (1 : 1 ratio) supplemented with 10,7 m androstenedione, 5 ,g/ml human apo-transferrin and 0.1% bovine serum albumin, in the presence or absence of FSH or LH (0, 1, 2, 4, 8, 16, 32 or 64 ng/ml each). Basal oestradiol-17, production by granulosa cells from small follicles reduced (p < 0.01) from days 1 to 2 of culture and became undetectable by day 3 and basal progesterone production increased (p < 0.05) from day 1 through day 4 of the culture. Although there was no effect of FSH on day 1 of the culture, FSH at 2, 4, 8 and 16 ng/ml increased (p < 0.05) oestradiol-17, production by granulosa cells from small follicles on day 2. Progesterone secretion was increased (p < 0.05) by all doses of FSH on all days of culture. All doses of LH had no effect on oestradiol-17, or progesterone production by granulosa cells from small follicles on any day of the culture. The results of this study demonstrate a serum-free culture system for buffalo granulosa cells and stimulatory effect of FSH but not LH on steroid hormone production by buffalo granulosa cells under these conditions. [source] An Inter-Subspecies Cloned Buffalo (Bubalus bubalis) Obtained by Transferring of Cryopreserved Embryos via Somatic Cell Nuclear TransferREPRODUCTION IN DOMESTIC ANIMALS, Issue 5 2010BZ Yang Contents The aim of this study was to explore the feasibility of cryopreservation of inter-subspecies cloned embryos in buffalo. In our experiment, river buffalo ear fibroblast nucleus was fused into swamp buffalo oocyte cytoplasm. The blastocyst formation rate for nuclear transfer of freshly thawed cells was not different from those of growing cells, confluent or serum-starved cells. A total of 122 cloned blastocysts derived from cryopreserved fibroblasts were cryopreserved and thawed, 37 were survived, the cryosurvival rate was 30.3%. The survived blastocysts were transferred into 15 recipient buffalos. Five of the recipients established pregnancy, but four of them aborted on day 53, 59, 145 and 179 of gestation respectively. One cross-bred buffalo (Murrah × Swamp buffalo (2n = 49) received three embryos delivered a 40.5 kg female calf by natural delivery on day 320 of gestation. Up to now (13-month old), the cloned calf has been growing well with no abnormity observed. These results demonstrated that cryopreservation of inter-subspecies cloned embryos is feasible to produce buffalo offspring. [source] In Vitro Culture and Differentiation of Buffalo (Bubalus bubalis) SpermatogoniaREPRODUCTION IN DOMESTIC ANIMALS, Issue 2 2010B Xie Contents The objective of this study was to develop a culture system which could support buffalo spermatogonia differentiation into spermatids in vitro. Testes from 3- to 5-month-old buffaloes were decapsulated and seminiferous tubules were enzymatically dissociated to recover spermatogonia and sertoli cells. The cells were cultured in modified Dulbecco modified Eagle medium supplemented with different concentrations of foetal bovine serum, retinol, testosterone for 2 months at 37°C. Spermatogonia and sertoli cells were identified with an antibody against c-kit or GATA4, respectively. The viability of spermatogonia in the media supplemented with different concentrations of serum was all significantly higher (p < 0.05) compared with that in the medium without serum. A-paired or A-aligned spermatogonia and spermatogonial colonies (AP-positive) were observed after 7,10 days of culture and spermatid-like cells with a flagellum (6,8 ,m) appeared after 30 days of culture. For cultured conditions, retinol could not significantly promote the formation of spermatid-like cells (p > 0.05), whereas supplementation of testosterone could significantly promote (p < 0.05) the formation of spermatid-like cells after 41 days of culture. The expression of the spermatid-specific marker gene (PRM2) was identified after 30 days of culture by RT-PCR. Yet, the transition protein 1 (TP1, a haploid makers) was not detected. Meanwhile, spermatids developed in vitro were also confirmed by Raman spectroscopy. These results suggest that buffalo spermatogonia could differentiate into spermatids in vitro based on the analysis of their morphology, PRM2 expression and Raman spectroscopy. Yet, the normality of the spermatid-like cells was not supported by TP1 expression. [source] Transcriptional Analysis of Buffalo (Bubalus bubalis) Oocytes During In Vitro Maturation Using Bovine cDNA MicroarrayREPRODUCTION IN DOMESTIC ANIMALS, Issue 1 2010OM Kandil Contents The need for improving in vitro production of buffalo embryos necessitates a better understanding of the molecular mechanisms regulating early development including oocyte maturation. Here, we used bovine cDNA microarray platform to investigate mRNA abundance of buffalo oocytes before and after in vitro maturation. For this, a total of six pools each contains 50 immature or in vitro matured buffalo oocytes were used for mRNA isolation and subsequent cDNA synthesis. The BlueChip bovine cDNA microarray (with approximately 2000 clones) was used to analyse gene expression profiles between immature and matured oocytes. Statistical analysis of microarray data revealed a total of 104 transcripts to be differentially expressed between the two oocyte groups. Among these, transcription factors (ZFP91), M-phase mitotic cell cycle (MPHOSPH9), growth factor (BMP15) and DNA binding (HMGN2) were found to be up-regulated in immature oocytes. Similarly, matured oocytes were found to be enriched with genes involved in cytoskeleton (ACTB), hydrogen ion transporting (ATP6V1C2) and structural constituent of ribosome (RPS27A). Quantitative real-time polymerase chain reaction validated the expression profile of some selected transcripts during array analysis. In conclusion, to our knowledge, this is the first large-scale expression study to identify candidate genes differentially abundant and with potential role during buffalo oocyte maturation. [source] Generation and Characterization of Embryonic Stem-Like Cell Lines Derived from In Vitro Fertilization Buffalo (Bubalus bubalis) EmbryosREPRODUCTION IN DOMESTIC ANIMALS, Issue 1 2010B Huang Contents In the present study, buffalo embryonic stem-like (ES-like) cell lines were successfully isolated, cultured and characterized. From a total of 92 normal buffalo embryos obtained by in vitro fertilization, 18 were morulae, 33 were blastocyst and 41 were hatched blastocyst, the inside of morulae or inner cell masses of blastocysts were isolated mechanically and cultured onto mitomocin-C-inactivated buffalo embryonic fibroblasts as feeder layers. Alkaline phosphatase (AP) of ES-like cells, as well as the specific stage embryonic antigen SSEA-1, SSEA-3, SSEA-4 and transcription factor OCT-4, was used to evaluate the characterization of the cells. The spontaneous differentiation of ES-like cells was induced by culturing on leukaemia inhibitory factor-free medium for more than 2 weeks without passage. To evaluate mark gene expression, total RNA was extracted from cells, and specific primers were used for reverse transcriptase-polymerase chain reaction (RT-PCR). After 8,10 days of culture, primary ES-like cell colonies were formed in 0% (0/18) of morulae, 24.24% (8/33) of blastocysts and 60.98% (25/41) of hatched blastocysts, respectively. The forming rate of primary ES-like cells colonies in hatched blastocyst group was significantly (p < 0.05) higher than the obtained for other groups. Two ES-like cell lines could survive to eight passages at least by using the method of mechanical dissociation, but just three passages by using the method of enzymatic dissociation. The cells formed large, multicellular colonies with distinct boundaries, exhibited many important features of ES/ES-like cells, including positive AP, SSEA-1, SSEA-3 and SSEA-4 activity. Undifferentiated buffalo ES-like cells expressed Oct-4, Nanog, Sox2 gene mRNA. In vitro differentiation experiments had demonstrated that those cells were pluripotent. [source] Factors Affecting the Quality of Cryopreserved Buffalo (Bubalus bubalis) Bull SpermatozoaREPRODUCTION IN DOMESTIC ANIMALS, Issue 3 2009SMH Andrabi Contents Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen,thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the major factors that may influence the post-thaw viability and fertility of buffalo spermatozoa are examined in detail. In addition, suggestions for improvement in cryogenic procedures for buffalo spermatozoa are also given. [source] Prognostic Value of Various Spermatological Attributes as Predictors of Zona Binding and Zona Penetration of Buffalo (Bubalus bubalis) SemenREPRODUCTION IN DOMESTIC ANIMALS, Issue 1 2009S Selvaraju Contents Twenty-four ejaculates from six (four ejaculates each) Surti buffalo bulls aged 4,8 years were used to assess various attributes of spermatozoa influencing the zona-binding and zona-penetration tests. Ejaculates from each bulls were subjected to in vitro sperm--zona binding and sperm--zona penetration tests (four replicates per bull) using immature buffalo oocytes. The average number of spermatozoa bound per oocyte was 27.79 ± 5.90. The average number of spermatozoa penetrated per oocyte was 3.35 ± 0.64. The average number of zona-bound and -penetrated spermatozoa differed significantly between animals. Significant difference (p < 0.05) was observed between the plasmalemma integrity as assessed by eosin--nigrosin stain and hypo-osmotic swelling (HOS) test. Furthermore, the percentage of cells positive for the HOS test, i.e. functional membrane integrity (51.25 ± 2.32) was significantly (p < 0.05) higher than hypo-osmotic swelling-Giemsa (HOS-G) test, i.e. the subpopulation of spermatozoa positive for functional membrane and acrosomal integrities (42.87 ± 4.56). The HOS test had significant correlations with plasmalemma integrity as measured by the vital stain, eosin--nigrosin (r = 0.85, p < 0.05). The HOS-G test also had significant correlation with plasmalemma integrity measured by vital stains such as eosin--nigrosin (r = 0.90, p < 0.05) and fluorogenic stains [carboxyfluorescein diacetate (CFDA) and propidium iodide (PI); r = 0.92, p < 0.01] and HOS test (r = 0.93), acrosomal integrity (r = 0.86, p < 0.05) and mitochondrial membrane potential (r = 0.99, p < 0.01). The plasmalemma integrity (fluorogenic stain), functional membrane integrity (HOS test), subpopulation of spermatozoa positive for functional membrane and acrosomal integrities (HOS-G test) and mitochondrial membrane potential had significant (p < 0.05) correlation with sperm zona binding and penetration. The present study indicates that these parameters could represent important determinants of sperm quality influencing zona binding and penetration. [source] Development of a Competitive Quantitative PCR Strategy for Evaluating the Expression Stability of 18s rRNA during In Vitro Maturation of Buffalo (Bubalus bubalis) Follicular OocytesREPRODUCTION IN DOMESTIC ANIMALS, Issue 2 2007APS Aswal Contents The present work describes the development of a quantitative competitive PCR strategy for quantifying the relative abundance of 18s rRNA transcripts in buffalo oocytes during in vitro maturation (IVM). As a method, the competitive PCR overcomes some of the shortcomings of conventional reverse transcriptase polymerase chain reaction (RT-PCR) procedure making it a more authentic quantitative method. A composite primer based approach was used to generate the competitor cDNA to be used as external control. Validity of the method for its efficiency was demonstrated by quantitative analysis of the competition parameters. Using this method the relative abundance of buffalo oocyte 18s rRNA transcript over the period of IVM was found to vary within a narrow range of 0.93,1.06 folds which establishes the accuracy of the method and reflects the stability of its expression during IVM. This qualifies the use of this house keeping gene as a valid internal control in studies investigating the gene expression pattern in buffalo oocytes. The competitive PCR approach described in this study could be used for quantification of other transcripts from a limited number of oocytes where a conventional RT-PCR method is either difficult to use or multiplexing it with highly abundant house keeping genes is apparently problematic. [source] Structural and Functional Features of Choroid Epithelium from Buffalo BrainTHE ANATOMICAL RECORD : ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, Issue 11 2007Gaetano Scala Abstract Choroid plexuses (CPs) play pivotal roles in many processes that establish, survey, and maintain the biochemical and cellular status of the central nervous system (CNS). Changes in the anatomy and physiology of CPs have been linked to several CNS diseases. However, CP structure and function are not definitely known. Here, we report structural and functional features of choroid epithelium from buffalo brain never described before. Mixed with common epithelial cells, two novel cell types were identified by scanning and transmission electron microscopies. The first peculiar cells showed a globular apical portion projecting into the ventricular cavities, and a basal peduncle in direct contact with blood capillaries underlying the epithelium. The second type of cells resulted to be formed by a globular body from which depart numerous processes; these cells, localized deeply in the choroid epithelium, strictly contact neighboring epithelial cells. No synaptic contacts were detected between these cell populations and common epithelial cells. To gain some insight into the functional properties of choroid cells, NADPH diaphorase (NADPHd) and neuronal nitric oxide synthase (nNOS) activities were evaluated. Of interest, whereas a strong NADPHd activity was detected in all cell types of choroid epithelium, nNOS was only detected in the first type of peculiar cells. The presence of nNOS in the CPs was confirmed by Western blotting. These results suggest that nitric oxide may serve as a signal for the regulation of CP multiple functions. Anat Rec, 290:1399,1412, 2007. © 2007 Wiley-Liss, Inc. [source] Pretreatment With Portal Venous Ultraviolet B Irradiated Donor Alloantigen Promotes Donor-Specific Tolerance to Rat Nerve Allografts,THE LARYNGOSCOPE, Issue 3 2001Eric M. Genden MD Abstract Objective To determine if a single intraportal inoculation of ultraviolet B-irradiated (UVB) donor splenocytes can prevent nerve allograft rejection and confer donor-specific immunotolerance to rat nerve allograft segments. Methods Age-matched, class I and class II major histocompatibility complex (MHC) mismatched Buffalo (RT1b) rats were transplanted with a syngeneic nerve isograft, a Lewis (RT1l) nerve allograft, or a Brown-Norway (RT1n) rat nerve allograft segment. Control Buffalo rats in group I received a 3.0-cm Lewis (RT11) sciatic-posterior tibial interposition nerve allograft without pretreatment;group II Buffalo rats received a syngeneic Buffalo nerve isograft without pretreatment. Group III Buffalo recipients were inoculated with 2.5 × 107 UVB-irradiated Lewis donor splenocyte cells by portal venous administration 7 days before transplantation with a 3.0-cm sciatic-posterior tibial nerve allograft from a Lewis (RT11) or a third party Brown-Norway rat (RT1n) donor (group IV). Nerve graft regeneration was assessed with walking track analysis, nerve conduction studies, retrograde neural tracing, nerve graft histology, and morphometry. Recipient immune tolerance was assessed through in vitro immunological assessment. Results Pretreatment with UVB-irradiated donor splenocytes 7 days before transplantation prevented nerve allograft rejection. Pretreated animals receiving a nerve allograft recovered limb function, and demonstrated morphological, histological, and electrophysiologic parameters of nerve regeneration similar to that measured in rats receiving a nerve isograft. In vitro immunological assessment by mixed lymphocyte culture (MLC), cytotoxic T lymphocyte (CTL) assay, limiting dilution analysis (LDA) of helper (pTH) and cytotoxic (pCTL) precursor frequencies, and IL-2 production demonstrated a marked donor-specific suppression in allografted animals pretreated with intraportal UVB-irradiated donor splenocytes. These assessments correlated with indefinite acceptance of donor nerve allografts. Conclusions A single pretreatment with a single intraportal dose of UVB-modified donor antigen specifically induces tolerance to peripheral nerve allografts in rats. [source] Eating and rumination behaviour in Brahman grade cattle and crossbred water buffalo fed on high roughage dietANIMAL SCIENCE JOURNAL, Issue 5 2010Renato S. A. VEGA ABSTRACT The purpose of this study is to compare feeding and other behavior and nutrient digestibility of tropical grade Brahman (body weight (BW) = 231 kg ± 12.4; n = 3) and crossbred water buffalo (BW = 300 kg ± 13.9; n = 3). This experiment on digestibility and measures of muscles of mastication utilized one-way, and animal behavior two-way, analysis of variance, respectively. Two video camcorders were installed in each pair of buffalo and Brahman for 24 h period programmed on the 107th, 109th and 111th days of the digestion trials. Frequency and duration of feeding, meal intake, rumination, bolus, chews, drinking, defecating, standing and lying were recorded daily. Muscle diameter of Digastricus, Masseter and Pterygoid and different regions of the tongue were sampled and measured under light microscope using a standard micrometer. Buffalo obtained significantly higher intake of dry matter, roughage, crude protein, total digestible nutrient and metabolized energy than Brahman. This was supported by longer meal duration (P , 0.05), and shorter meal breaks (P , 0.05) of buffalo than Brahman. The diameter of the muscles for mastication was bigger (P , 0.05) in buffalo than in Brahman, which is indicative of stronger chewing ability. Briefly, lesser and slower chewing action; higher intake of roughage and crude protein; and longer resting behavior of crossbred water buffalo than Brahman are all indicative of better digestive and metabolic performance of the buffalo under high roughage feeding conditions. [source] Comparative dry matter intake and nutrient utilisation efficiency in lactating cattle and buffaloesJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 4 2003Shyam S Paul Abstract An attempt was made to assess the comparative dry matter intake and nutrient utilisation efficiency of lactating buffaloes and cows based on results obtained from experimental feeding trials conducted in India. Data on dry matter (DM) intake, total digestible nutrient (TDN) intake, digestible crude protein (DCP) intake, body weight or metabolic body size (MBS), body weight change, milk yield or 4% fat-corrected milk (FCM) yield, milk fat percentage and roughage/concentrate ratio in the diet of lactating buffaloes and cows were collected from published reports. The data were processed and analysed to assess the comparative dry matter intake and nutrient utilisation efficiency using suitable statistical analysis models. DM intake was significantly (P,<,0.01) lower in buffaloes (2.57,kg DM per 100,kg body weight or 119.2,g,kg,1 MBS) than in cattle (3.09,kg DM per 100,kg body weight or 132.0,g,kg,1 MBS). Mean gross energetic efficiency (30.53 versus 27.83%; P,<,0.01), gross protein efficiency (45.48 versus 37.06%; P,<,0.01), net energetic efficiency (69.16 versus 64.10%; P,<,0.05) and net protein efficiency (80.15 versus 59.59%; P,<,0.001) were significantly higher in buffaloes than in cattle. Lactating buffaloes consumed significantly less (P,<,0.001) protein (75.69,g DCP) and less (P,<,0.01) energy (695.9,g TDN) than cows (93.89,g DCP, 774.8,g TDN) for production of 1,kg of 4% fat-corrected milk. Buffaloes had higher energy and protein utilisation efficiencies as compared with cattle at similar fat-corrected milk production level, plane of energy and protein nutrition, body size and body weight change. Buffaloes (1.189,kg DM,kg,1 4% FCM) consumed a similar (P,>,0.05) amount of feed dry matter to that of cows (1.267,kg DM,kg,1 4% FCM). However, when DM intake,kg,1 FCM (4%) was compared at constant levels of metabolic body size, fat-corrected milk, body weight change, dietary energy concentration and green forage percentage in the diet, lactating buffaloes consumed significantly less DM,kg,1 FCM yield as compared with cattle. It was concluded that DM intake was lower in lactating buffaloes. Moreover, lactating buffaloes utilised dietary dry matter, energy and protein for milk production more efficiently than cattle. © 2003 Society of Chemical Industry [source] Fodder production and soil health with conjunctive use of saline and good quality water in ustipsamments of a semi-arid regionLAND DEGRADATION AND DEVELOPMENT, Issue 2 2007R. K. Yadav Abstract Food and fodder shortage in arid and semi-arid regions force farmers to use marginal quality water for meeting the water requirement of crops which result in low quality, reduced production and an adverse impact on soil properties. A field study on loamy-sand (Hyperthermic Typic Ustipsamments) saline soil was conducted during 1999,2001 at Central Institute for Research on Buffaloes, Hisar. This involved assessment of effects of conjunctive use of saline water, EC,=,4·6,7·4,dSm,1, SAR,=,14,22 ((mmol,1)½ with good quality water on five fodder crop rotations: oat-sorghum (Avena sativa- ,Sorghum bicolor), rye grass,sorghum (Loleum rigidum,Sorghum bicolor), Egyptian clover,sorghum (Trifoleum alexandrinum,Sorghum bicolor), Persian clover,sorghum (Trifoleum resupinatum,Sorghum bicolor) and Indian clover,sorghum (Melilotus indica,Sorghum bicolor) and certain soil properties associated with it. Leguminous winter fodder crops were more sensitive to poor quality water use. Reductions in fodder yield with use of saline water alone throughout season were 85, 68, 54, 42, 36 and 26 per,cent in Indian clover, Egyptian clover, Persian clover, oat, rye grass and sorghum respectively as compared to good quality water. Leguminous fodder crops produced protein rich (12,14 per,cent) and low fibre (18,20 per,cent) fodder as compared to poor quality grassy fodder under good quality water irrigation but their quality deteriorated when saline water was used. These leguminous crops accumulated proportionately higher Na+ (1·58 per,cent) resulting in adverse impact on their growth as compared to grassy fodder crops. Higher soil salinity (12·2,dSm,1), SAR,=,20 (mmol,1)½ was recorded with saline water irrigation; and slight adverse impact was noticed on infiltration rate and contents of water dispersible clay. Alternate cyclic use of canal and saline water could be an option for fodder production under such conditions. Copyright © 2006 John Wiley & Sons, Ltd. [source] Forms of Reproductive Disorders in Cattle and Buffaloes in Middle EgyptREPRODUCTION IN DOMESTIC ANIMALS, Issue 4 2009A Ali Contents The objective of this study was to investigate the common forms of reproductive disorders and trends of fertility in cattle and buffaloes in Middle Egypt, and investigate nutritional deficiencies or imbalances as a cause of infertility. During the period from 2003 to 2006 a total of 4276 animals (2755 cattle and 1521 buffaloes) were examined, during winter and summer at three districts of Middle Egypt. Based on the owner complains, animals were categorized as anestrum, repeat breeder and those for pregnancy diagnosis. Feedstuffs of these districts were assessed for nutrient and mineral contents. Animals were examined by rectal palpation and by transrectal ultrasonography. The results showed that, ovarian inactivity was the most common cause of anestrum, whereas endometritis was the main frequent finding of repeat breeding. District, season and year affected the incidences of infertility. The pregnancy rate remained constant (cattle) or increased (buffaloes) from 2003 to 2006. The incidence of ovarian inactivity in both species decreased over the same period. In winter, the commonly used feedstuffs would be adequate to supply the animals with needs, but in summer, rations seem to be deficient in many essential nutrients. In conclusion, ovarian inactivity is the main cause of infertility in cattle and buffaloes in Middle Egypt. There is a link between poor nutrition in summer and the high incidence of ovarian inactivity. Fertility trends during the past few years are encouraging. [source] Ovum Pick-up in Cycling and Lactating Postpartum Swamp Buffaloes (Bubalis bubalis)REPRODUCTION IN DOMESTIC ANIMALS, Issue 2 2005A Promdireg Contents The objective of this study was to evaluate the efficiency of Ovum Pick Up (OPU) in cycling (n = 5) and lactating, postpartum, swamp buffaloes (n = 6) with and without gonadotropin stimulation. The OPU was performed every two weeks in all groups of animals, for a total of six sessions. Thirty collections were performed in five cycling buffaloes and 36 collections in six lactating postpartum buffaloes. Buffaloes that received hormonal stimulation were given a total of 400 mg, follicle stimulating hormone (FSH), administered twice daily over 3 days in decreasing doses, together with 100 ,g of GnRH, 24 h after the last FSH injection. Following a resting period of 1 month, the two groups of buffaloes, were subjected to the same OPU regimen, but without any hormonal treatment for an additional six OPU sessions. The number of aspirated follicles recorded from the hormonal stimulated, cycling animals and lactating, postpartum buffaloes was not significantly different, 7.2 ± 3.7 and 9.0 ± 3.2, respectively (p > 0.05). Recovered oocytes collected from the two groups of hormonally stimulated animals were also not statistically different: 3.7 ± 2.7 in the cycling and 5.9 ± 3.5 in the lactating postpartum group (p > 0.05). In the two groups of buffaloes not receiving hormonal stimulation, the number of aspirated follicles was not significantly different: 2.1 ± 1.4 and 1.4 ± 0.7 in cycling and lactating postpartum buffaloes respectively (p > 0.05). Recovered oocytes in the non-treated groups were also similar: 1.4 ± 1.3 vs 0.7 ± 0.8 in cycling and lactating buffaloes (p > 0.05). Among stimulated buffaloes, most aspirated follicles were small in size (,5 mm), whereas they were mostly medium and large sizes in the non-treated buffaloes. The oocyte recovery rate in both the groups, cycling and lactating postpartum, were 51.6% and 69.5% in stimulated groups and 55.0% and 53.1% in non-stimulated groups (p > 0.05). The majority of recovered oocytes were single- and multi-layered, and the number was greater in the cycling than in the lactating, postpartum buffaloes. The number and quality of recovered oocytes was similar in all groups of buffaloes whether they were received or did not receive hormonal stimulation. Moreover no difference was found in multi- and single-layered oocytes between cycling and lactating, postpartum buffaloes. In conclusion, OPU can be performed successfully in swamp buffalo in different reproductive status and FSH administration was shown to increase the number of aspirated oocytes in both cycling and lactating, postpartum buffaloes. [source] Playing the Stockmarket in Tana TorajaTHE AUSTRALIAN JOURNAL OF ANTHROPOLOGY, Issue 1 2000Robyn Thompson This paper describes the players and the play in a weekly stockmarket in Tana Toraja. South Sulawesi, where up to 600 buffalo bulls are bought and sold for exorbitant prices by any standards. These prices are partly determined by external, global economic forces. The buffaloes are all intended for sacrifice in elaborate funerals. Although contributed by individuals or families they are conceived as part of mana. the common wealth of tongkonan. the origin houses of the Toraja. Live bulls cannot be given away but are able to be lent in ceremonial exchanges at funerals. After sacrifice, their raw meat is distributed to participants in the funeral in a version of potlatch. Bulls mediate all exchange. apart from mundane commodity exchange, and are liquid assets. A mature black bull is an object of general equivalence able to be exchanged as payment for certain symbolic objects. In the past the production, distribution and circulation of buffaloes, both on the hoof and as meat, were controlled by the nobility. Buffaloes were said to be in finite supply. They derived from the Upperworld and accompanied the ancestors of the Toraja nobility to this world and were replenished through the ritual of the ways of the ancestors. Now the advent of the market has democratised buffaloes. There has been a dispersion of wealth and the power that the bulls embody. Any man,noble, commoner or former slave-who has sufficient cash is able to buy and sell buffaloes: to have a share in the stockmarket. The marketplace has become a new field of power play, one where innovative methods are being found for increasing the supply of buffaloes by importing hundreds of inferior quality buffaloes and by a program of artificial insemination which has been instigated by the local government. In the parlance of Wall Street this is a bull market. [source] Microvascular Architecture of the Near-term Uterine Caruncles in Water Buffaloes (Bubalus bubalis)ANATOMIA, HISTOLOGIA, EMBRYOLOGIA, Issue 3 2007M. M. M. Abd-Elnaeim Summary The present investigation was carried out on five near-term pregnant water buffaloes for studying the microvascular architecture of the uterine caruncles. The vascular casts were obtained by injection of 4:1 mixture of mercox and methylmethacrylate through the branches of the uterine arteries. After complete polymerization of the plastic, corrosion was conducted in 20% potassium hydroxide, then the vessel casts were immersed in distilled water, cut into small pieces, sputter coated with gold, and examined by using a scanning electron microscope. The buffalo uterine caruncle is highly vascularized through two slightly convoluted arteries and a single less tortuous vein. The arteries branch into several stem arteries at the base of the uterine caruncle, which follow nearly straight course in the primary septa towards the fetal side. During the courses of these stem arteries arterioles of variable diameters arise. The arterioles run in the secondary and tertiary septae and at this location arterioles and venules are connected through a voluminous capillary complex. The latter consists of capillaries of greatly variable diameters with vigorous coiling and sinusoidally dilated zones. From the capillary complexes the blood is driven through postcapillary venules back to the tertiary, secondary and primary septa, respectively, and then converge into stem veins which leave the caruncles through the branches of the uterine vein. [source] What do dung beetles eat?ECOLOGICAL ENTOMOLOGY, Issue 6 2007PETER HOLTER Abstract 1.,Most adult coprophagous beetles feed on fresh dung of mammalian herbivores, confining ingestion to small particles with measured maximum diameters from 2,5 to 130 ,m, according to body size and kind of beetle. This study explores benefits and costs of selective feeding in a ,typical' dung beetle with a maximum diameter of ingested particles (MDIP) of 20 ,m. 2.,Examined dung types (from Danish domestic sheep, cattle and horse, and African wild buffalo, white rhino and elephant) contained 76,89% water. Costs of a 20 ,m MDIP were often low, since 69,87% of the total nitrogen in bulk dung other than that of elephant and rhino (40,58%) was available to selective feeders. 3.,Nitrogen concentrations were high , and C/N ratios low , in most types of bulk dung compared with the average food of terrestrial detritivores or herbivores. Exceptions were elephant and rhino dung with low nitrogen concentrations and high C/N ratios. 4.,Estimated C/N ratios of 13,39 in bulk dung (sheep,elephant) were decreased by selective feeding to 7.3,12.6 in the ingested material. In assimilated food, ratios are probably only 5,7, as most assimilable nitrogen and carbon may be of microbial origin. If so, the assimilable food contains a surplus of nitrogen relative to carbon. 5.,The primary advantage of selective feeding, particularly in dung with a high C/N ratio, may be to concentrate assimilable carbon in the ingested food. Effects of changing the MDIP within 20,106 ,m are modest, especially in dung with a low C/N ratio. [source] Tyrosine phosphorylation of a 38-kDa capacitation-associated buffalo (Bubalus bubalis) sperm protein is induced by L -arginine and regulated through a cAMP/PKA-independent pathwayINTERNATIONAL JOURNAL OF ANDROLOGY, Issue 1 2008S. C. Roy Summary The aim of the present study was to determine the effect of l -arginine on nitric oxide (NO,) synthesis, capacitation and protein tyrosine phosphorylation in buffalo spermatozoa. Ejaculated buffalo spermatozoa were capacitated in the absence or presence of heparin, or l -arginine or N, -nitro- l -arginine methyl ester (l -NAME), an inhibitor of nitric oxide synthase (NOS) for 6 h. Capacitating spermatozoa generated NO, both spontaneously and following stimulation with l -arginine and l -NAME quenched such l -arginine-induced NO, production. Immunolocalization of NOS suggested for existence of constitutive NOS in buffalo spermatozoa. l -Arginine (10 mm) was found to be a potent capacitating agent and addition of l -NAME to the incubation media attenuated both l -arginine and heparin-induced capacitation and suggested that NO, is involved in the capacitation of buffalo spermatozoa. Two sperm proteins of Mr 38 000 (p38) and 20 000 (p20) were tyrosine phosphorylated extensively by both heparin and l -arginine. Of these, the tyrosine phosphorylation of p38 was insensitive to both induction by cAMP agonists as well as inhibition by a protein kinase A (PKA) inhibitor. Further, most of these l -arginine-induced tyrosine phosphorylated proteins were localized to the midpiece and principal piece regions of flagellum of capacitated spermatozoa and suggested that sperm flagellum takes active part during capacitation. These results indicated that l -arginine induces capacitation of buffalo spermatozoa through NO, synthesis and tyrosine phosphorylation of specific sperm proteins involving a pathway independent of cAMP/PKA. [source] | |||||||||||||||||||||||||||||||||||||||||||