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Bone Specimens (bone + specimen)
Selected AbstractsSinus Lift Using a Nanocrystalline Hydroxyapatite Silica Gel in Severely Resorbed Maxillae: Histological Preliminary StudyCLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH, Issue 2009Luigi Canullo DDS ABSTRACT Purpose: The aim of this preliminary study was to evaluate histologically a nanocrystalline hydroxyapatite silica gel in maxillary sinus floor grafting in severely resorbed maxillae. Materials and Methods: A total of 16 consecutive patients scheduled for sinus lift were recruited during this study. Patients were randomly divided in two groups, eight patients each. In both groups, preoperative residual bone level ranged between 1 and 3 mm (mean value of 2.03 mm). No membrane was used to occlude the buccal window. Second surgery was carried out after a healing period of 3 months in Group 1 and 6 months in Group 2. Using a trephine bur, one bone specimen was harvested from each augmented sinus and underwent histological and histomorphometric analysis. Results: Histological analysis showed significant new bone formation and remodeling of the grafted material. In the cores obtained at 6 months, regenerated bone, residual NanoBone, and bone marrow occupied respectively 48 ± 4.63%, 28 ± 5.33%, and 24 ± 7.23% of the grafted volume. In the specimens taken 3 months after grafting, mean new bone was 8 ± 3.34%, mean NanoBone was 45 ± 5.10%, and mean bone marrow was 47 ± 6.81% of the bioptical volume. Conclusions: Within the limits of this preliminary prospective study, it was concluded that grafting of maxillary sinus using nanostructured hydroxyapatite silica gel as only bone filler is a reliable procedure also in critical anatomic conditions and after early healing period. [source] Bone lesions: Role of sediment cytologyDIAGNOSTIC CYTOPATHOLOGY, Issue 6 2009Surbhi Shah M.B.B.S. Abstract This study was conducted to evaluate the role of sediment cytology of biopsy specimen fixatives, which is usually discarded, in early diagnosis of bone lesions. Cytological smears prepared from sediments of biopsy specimen fixatives (sediment cytology) were used to study 65 bone specimens biopsied with suspicion of malignancy. The cytological diagnosis was then compared with histological diagnosis, taking the latter as gold standard. Smears were adequately cellular and showed good preservation of cellular morphology. Some of the smears showed microfragments of tissue. Cytology labeled 29 lesions as malignant, 26 lesions as benign, 3 as inflammatory, and 7 smears as inconclusive because of low cell yield. Sediment cytology was able to correctly diagnose 58 of 65 lesions. There was no false-positive or false-negative case. The sediment cytology could be considered as an easy and effective diagnostic tool that can provide early diagnosis for the lesion of bone. Diagn. Cytopathol. 2009. © 2009 Wiley-Liss, Inc. [source] The contribution of limb bone fracture patterns to reconstructing early hominid behaviour at Swartkrans cave (South Africa): archaeological application of a new analytical methodINTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 4 2005T. R. Pickering Abstract Recently, Alcántara García et al. (in press) presented a new method and criteria for distinguishing between fractures imparted by hominid hammerstone percussion and carnivores chewing on ,green' limb bones of ungulates. The method uses a combination of fracture plane and fracture angle data that are useful for elucidating the relative role of hominids in the accumulation of prehistoric archaeofaunas, especially when employed in concert with other classes of taphonomic data. We briefly summarise the method and apply it to the ungulate limb bone subassemblage from Swartkrans Member 3, a c. 1.0 million year old site from South Africa that preserves Early Stone Age lithic artefacts, hominid fossils, and an abundant mammalian fauna with cutmarked, hammerstone-percussed and burned bone specimens. Results of the fracture pattern analysis corroborate indications from other lines of taphonomic data that there was minimal carnivore,hominid interdependence in the formation of the fauna, and that carnivores were probably responsible for the majority of the bone collection in Member 3. However, we also document a significant hominid influence on assemblage formation, a finding that expands and refines our understanding of large animal carcass foraging by hominids in southern Africa during the early Pleistocene. Copyright © 2004 John Wiley & Sons, Ltd. [source] A Three-Dimensional Simulation of Age-Related Remodeling in Trabecular Bone,JOURNAL OF BONE AND MINERAL RESEARCH, Issue 4 2001J. C. Van Der Linden Abstract After peak bone mass has been reached, the bone remodeling process results in a decrease in bone mass and strength. The formation deficit, the deficit of bone formation compared with previous resorption, results in bone loss. Moreover, trabeculae disconnected by resorption cavities probably are not repaired. The contributions of these mechanisms to the total bone loss are unclear. To investigate these contributions and the concomitant changes in trabecular architecture and mechanical properties, we made a computer simulation model of bone remodeling using microcomputed tomography (micro-CT) scans of human vertebral trabecular bone specimens. Up to 50 years of physiological remodeling were simulated. Resorption cavities were created and refilled 3 months later. These cavities were not refilled completely, to simulate the formation deficit. Disconnected trabeculae were not repaired; loose fragments generated during the simulation were removed. Resorption depth, formation deficit, and remodeling space were based on biological data. The rate of bone loss varied between 0.3% and 1.1% per year. Stiffness anisotropy increased, and morphological anisotropy (mean intercept length [MIL]) was almost unaffected. Connectivity density increased or decreased, depending on the remodeling parameters. The formation deficit accounted for 69,95%, disconnected trabeculae for 1,21%, and loose fragments for 1,17% of the bone loss. Increasing formation deficit from 1.8% to 5.4% tripled bone loss but only doubled the decrease in stiffness. Increasing resorption depth from 28 to 56 ,m slightly increased bone loss but drastically decreased stiffness. Decreasing the formation deficit helps to prevent bone loss, but reducing resorption depth is more effective in preventing loss of mechanical stiffness. [source] Influence of bone density on the cement fixation of femoral hip resurfacing componentsJOURNAL OF ORTHOPAEDIC RESEARCH, Issue 8 2010Rudi G. Bitsch Abstract In clinical outcome studies, small component sizes, female gender, femoral shape, focal bone defects, bad bone quality, and biomechanics have been associated with failures of resurfacing arthroplasties. We used a well-established experimental setup and human bone specimens to analyze the effects of bone density on cement fixation of femoral hip resurfacing components. Thirty-one fresh frozen femora were prepared for resurfacing using the original instruments. ASRÔ resurfacing prostheses were implanted after dual-energy X-ray densitometer scans. Real-time measurements of pressure and temperature during implantation, analyses of cement penetration, and measurements of micro motions under torque application were performed. The associations of bone density and measurement data were examined calculating regression lines and multiple correlation coefficients; acceptability was tested with ANOVA. We found significant relations between bone density and micro motion, cement penetration, cement mantle thickness, cement pressure, and interface temperature. Mean bone density of the femora was 0.82,±,0.13,g/cm2, t- score was ,0.7,±,1.0, and mean micro motion between bone and femoral resurfacing component was 17.5,±,9.1,µm/Nm. The regression line between bone density and micro motion was equal to ,56.7,×, bone density,+,63.8, R,=,0.815 (p,<,0.001). Bone density scans are most helpful for patient selection in hip resurfacing, and a better bone quality leads to higher initial component stability. A sophisticated cementing technique is recommended to avoid vigorous impaction and incomplete seating, since increasing bone density also results in higher cement pressures, lower cement penetration, lower interface temperatures, and thicker cement mantles. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:986,991, 2010 [source] Early healing of flexor tendon insertion site injuries: Tunnel repair is mechanically and histologically inferior to surface repair in a canine modelJOURNAL OF ORTHOPAEDIC RESEARCH, Issue 5 2006Matthew J. Silva Abstract Orthopedic injuries often require surgical reattachment of tendon to bone. Tendon ends can be sutured to bone by direct apposition to the bone surface or by placement within a bone tunnel. Our objective was to compare early healing of a traditional surface versus a novel tunnel method for repair of the flexor digitorum profundus (FDP) tendon insertion site in a canine model. A total of 70 tendon,bone specimens were analyzed 0, 5, 10 or 21 days after injury and repair, using tensile and range of motion mechanical testing, histology and densitometry. Ultimate force (a measure of repair strength) did not differ between surface and tunnel repairs at day 0. Both repair types had reduced strength at 10 and 21 days compared to 0 days, indicative of deterioration of suture grasping strength (tendon softening). At 21 days, tendons repaired in a bone tunnel had 38% lower ultimate force compared to surface repairs (p,=,0.017). Histological findings were comparable between repair groups at 5 and 10 days but differed at 21 days, when we saw evidence of maturation of the tendon,bone interface in the surface repairs compared to an immature fibrous interface with no evidence of tendon,bone integration in the tunnel repairs. After accounting for bone removed by the tunnel, no difference in bone mineral density or trabecular bone volume existed between surface and tunnel repairs. If the results of our animal study extend to healing of the human FDP insertion, they indicate that FDP tendons should be reattached to the distal phalanx by suture to the cortical surface rather than suture in a bone tunnel. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res [source] Creep dominates tensile fatigue damage of the cement,bone interfaceJOURNAL OF ORTHOPAEDIC RESEARCH, Issue 3 2004Do-Gyoon Kim Abstract Fatigue damage from activities of daily living has been considered to be a major cause of aseptic loosening in cemented total hip arthroplasty. The cement,bone interface is one region where loosening could occur, but to date the fatigue response of the interface has not been examined. Cement,bone specimens were prepared from fresh frozen human cadaver tissue using simulated in vivo conditions. Tensile fatigue tests to failure were performed in an environmental chamber. Loss of specimen stiffness (stiffness damage) and permanent displacement after unloading (creep damage) were found in all specimens. At failure, creep damage accounted for the majority (79.9 ± 10.6%) of the total strain damage accumulation at failure (apparent strain, , = 0.0114 ± 0.00488). A power law relationship between strain-damage rate and time-to-failure showed that the strain-damage rate was an excellent predictor of the fatigue life of the cement,bone interface. The S,N response of the interface was obtained as a function of the applied stress ratio and the initial apparent strain. The total motion between cement and bone (72.2 ± 29.8 ,m) prior to incipient failure due to both stiffness and creep fatigue damage may be sufficient to result in fibrous tissue formation and contribute to eventual clinical loosening. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved. [source] Brief communication: Paleohistopathological analysis of pathology museum specimens: Can periosteal reaction microstructure explain lesion etiology?AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, Issue 1 2009Darlene A. Weston Abstract The assertion that the microstructure of periosteal new bone formation can be used to differentiate between disease etiologies (Schultz: Yrbk Phys Anthropol 44 2001 106,147; Schultz: Identification of pathological conditions in human skeletal remains, 2nd ed. London: Academic Press 2003 73,109) was tested in a pilot-study, using diagnosed bone specimens from St George's Hospital Pathology Museum, London, UK. Embedded bone specimens exhibiting pathological periosteal new bone formation were examined using scanning electron microscopy in back-scattered electron imaging mode (SEM-BSE). The results suggest that several histological features (i.e. Grenzstreifen, Polsters, and sinuous lacunae) deemed to be diagnostic of specific pathological conditions are of no specific diagnostic value, as they are encountered in pathological conditions of differing disease etiology. These results tie in with a previous investigation demonstrating a lack of diagnostic qualitative or quantitative characteristics seen in the macroscopic and radiographic appearance of periosteal reactions (Weston: Am J Phys Anthropol 137 2008 48,59). Am J Phys Anthropol, 2009. © 2009 Wiley-Liss, Inc. [source] Ancient DNA and Family Relationships in a Pompeian HouseANNALS OF HUMAN GENETICS, Issue 4 2009Giovanni Di Bernardo Summary Archaeological, anthropological and pathological data suggest that thirteen skeletons found in a house at the Pompeii archaeological site, dated to 79 A.D., belong to one family. To verify this and to identify the relationships between these individuals, we analyzed DNA extracted from bone specimens. Specifically, hypervariable segment 1 (HVS1) of the human mitochondrial DNA (mtDNA) control region was amplified in two overlapping polymerase chain reactions and the sequences were compared to the revised Cambridge Reference Sequence. As independent controls, other polymorphic sites in HVS1, HVS2 and in the coding region were analyzed. We also amplified some short tandem repeats of the thirteen specimens. This study revealed that six of the thirteen individuals are indeed closely related. [source] Mitotic arrest defective protein 2 expression abnormality and its clinicopathologic significance in human osteosarcomaAPMIS, Issue 3 2010LING YU Yu L, Guo W-C, Zhao S-H, Tang J, Chen J-L. Mitotic arrest defective protein 2 expression abnormality and its clinicopathologic significance in human osteosarcoma. APMIS 2010; 118: 222,29. Osteosarcoma is the most common primary malignancy of bone. Overexpression of mitotic arrest defective protein 2 (MAD2) is found in many human neoplasms, but its role in the oncogenesis of osteosarcoma is an untouched topic. The objective of this research was to observe the expression of MAD2 in human osteosarcoma and explore its clinicopathologic significance. MAD2 expression was analyzed in 48 primary osteosarcoma cases (19 osteoblastic osteosarcomas, 17 chondroblastic osteosarcomas and 12 fibroblastic osteosarcomas) using immunohistochemistry. A total of 20 normal bone specimens formed a control group. MAD2 was commonly overexpressed in human osteosarcoma. Immunopositivity was higher in tumors with lower differentiation and higher clinical stage. Increased expression of MAD2 was associated with earlier metastasis and poorer survival. Our findings provide evidence that MAD2 contributes to the pathogenesis and development of human osteosarcoma, Testing may have a clinical role in predicting prognosis, selecting appropriate chemotherapeutic strategies and providing novel strategies for osteosarcoma therapy. [source] Immunohistochemical profile of ephrin A4 expression in human osteosarcomaAPMIS, Issue 4 2009ASMAA GABER ABDOU Ephrin receptors and ephrin ligands constitute one of the largest groups of tyrosine kinases. The division of ephrin receptors into type A or type B is determined by their ligand-binding specificities. Ephrin A4 as a ligand has a broad capacity to bind and stimulate different subtypes of ephrin A receptors. Little is known about the role of ephrins generally and ephrin A4 particularly in osteosarcoma. Ephrin A4 was immunohistochemically assessed on archival material from 46 primary osteosarcoma cases, 10 metastatic pulmonary lesions and 20 normal control bone specimens. Ephrin A4 was expressed in 100% of normal bone specimens, in 84.4% of primary osteosarcoma cases and in all metastatic pulmonary lesions. Cytoplasmic and nucleocytoplasmic patterns of ephrin A4 immunoreactivity were observed, with the predominance of the latter pattern in normal bone (100%), and in 43.5% of primary osteosarcoma cases, which showed a higher intensity of expression compared with normal bone (p<0.05). The cytoplasmic pattern is the only staining pattern seen in metastatic cases, which may suggest its role in enhancement of invasion and metastasis. The differences in the distribution of the two patterns of ephrin A4 may indicate a different biological activity of this molecule depending on its localization. The nuclear localization of ephrin A4 requires further investigation to clarify the mechanism and the significance of the nuclear trafficking of ephrin A4. [source] Effects of Screw Eccentricity on the Initial Stability of the Acetabular Cup in Artificial Foam Bone of Different QualitiesARTIFICIAL ORGANS, Issue 1 2010Jui-Ting Hsu Abstract Acetabular cup loosening is one of the major failure models of total hip replacement (THR), which is mostly due to insufficient initial stability of the cup. Previous studies have demonstrated that cup stability is affected by the quality of the host bone and the surgical skill when inserting screws. The purpose of this study was to determine the effects on the initial stability of the acetabular cup of eccentric screws in bone of different qualities. In this study, hemispherical cups were fixed into bone specimens constructed from artificial foam with three elastic moduli using one to three screws. The effects of two types of screw eccentricity (offset and angular) on the stability of the acetabular cup were also evaluated. The experimental results indicate that in the presence of ideal screwing, the cup was stable in bone specimens constructed from foam with the highest elastic modulus. In addition, increasing the number of ideal screws enhanced the cup stability, especially in bone specimens constructed from soft foam. Moreover, the cup stability was most affected by offset eccentric screw(s) in the hard-foam bone specimens and by angular eccentric screw(s) in the soft-foam bone specimens. The reported results indicate that the presence of screw eccentricity affects the initial stability of the acetabular cup. Surgeons should keep this in mind when performing screw insertions in THR. However, care is necessary when translating these results to the intraoperative situation due to the experiments being conducted under laboratory conditions, and hence, future studies should attempt to replicate the results reported here in vivo. [source] Effect of donor characteristics, technique of harvesting and in vitro processing on culturing of human marrow stroma cells for tissue engineered growth of boneCLINICAL ORAL IMPLANTS RESEARCH, Issue 5 2005Helge Bertram Abstract: The aim of this study was to assess the effect of donor characteristics and the technique of harvesting and in vitro processing on the efficacy of culturing of human mesenchymal stem cells (hMSCs) for tissue engineered growth of bone. Cultures of hMSCs were derived from iliac crest bone marrow aspirates (21 donors, age 11,76) and from cancellous bone grafting material (32 donors, age 13,84). Age had no significant effect on the ability to isolate and culture hMSCs, although the failure rate was 55.6% in donors beyond the age of 60, while it varied between 14.3% and 22.2% in donors under 60 years of age. Male and female donors had comparable failure rates (27.3% and 28.6%, respectively). Culturing of hMSCs was successful in 90.4% of marrow aspirates from 21 donors and in 62.5% of cancellous bone specimens from 35 donors. This difference was statistically significant (P=0.023). Regression analysis confirmed that at simultaneous testing of the three variables, only the source of cells significantly affected the result (P=0.043). Morphological evaluation of the unfractionated primary population showed a change in cell shape of the adherent cells from a triangular into thin spindle-shaped elongated form, which remains until confluence. When the cultures were exposed to osteoinductive medium, various morphotypes expressing different levels of alkaline phosphatase and secreting different amounts of mineral were evident. Morphology of marrow stroma cells (MSCs) from marrow aspirates was not different from MSCs derived from cancellous bone specimens. Expression of osteogenic markers in MSCs as shown by PCR as well, did not differ between the two sources. It is concluded that marrow aspirates and cancellous bone specimens produce comparable populations of MSCs. However, bone marrow aspirates from donors under the age of 60 years rather than cancellous bone chips are favourable for isolation and expansion of hMSCs for tissue engineered growth of bone. Résumé Le but de cette étude a été d'analyser l'effet des caractéristiques du donneur et la technique de prélèvement et du processus in vitro sur l'efficacité des cultures des cellules souches mésenchymateuses humaines (hMSC) pour la croissance tissulaire en laboratoire de l'os. Les cultures de hMSC provenaient de la moelle osseuse de la crête iliaque (21 donneurs de 11 à 76 ans) et de matériel de greffe osseuse d'os spongieux (32 donneurs de 13 à 84 ans). L'âge n'avait aucun effet significatif sur l'habilitéà isoler et à mettre en culture les hMSC bien que le taux d'échec était de 56% chez les donneurs au-dessus de 60 ans et variait de 14 à 22% chez les donneurs en-dessous de 60 ans. Les hommes et les femmes avaient des taux d'échec comparables de respectivement 27 et 28%. La mise en culture des hMSC a réussi dans 90% des prélèvements de moelle et dans 63% dans les échantillons osseux spongieux. Cette différence était statistiquement significative (P=0,023). L'analyse de régression a confirmé qu'avec un test simultané des trois variables, seul la source de cellules avait un réel effet sur le résultat (P=0,043). L'évaluation morphologique de la population primaire non-fractionnée a montré un changement dans l'apparence des cellules adhérentes depuis un aspect triangulaire à une forme allongée en forme de fuseau qui restait jusqu'à confluence. Lorsque les cultures étaient exposées à un milieu ostéoinductif différents morphotypes exprimant différents niveaux de phosphatase alcaline et secrétant différentes quantitées de minéraux étaient mis en évidence. La morphologie des MSC des prélèvements de la moelle n'était pas différente des MSC provenant des spécimens osseux spongieux. L'expression des marqueurs ostéogéniques dans les MSC ne montrait par PCR aucune différence entre ces deux sources. Les prélèvements de moelle et les spécimens osseux spongieux produisent donc des populations comparables de MSC. Cependant, les aspirations de moelle de donneurs en-dessous de 60 ans plutôt que les chips osseux spongieux sont favorables pour l'isolation et l'expansion des hMSC pour la croissance osseuse en laboratoire. Zusammenfassung Es war das Ziel dieser Studie, den Einfluss von Spender-Merkmalen, Entnahmetechnik und in vitro -Behandlung bei der erfolgreichen Kultivierung von menschlichen mesenchymalen Stammzellen (hMSCs) für das gewebsgesteuerte Knochenwachstum zu untersuchen. Die Kulturen der hMSCs gewann man aus vom Beckenkamm aspiriertem Knochenmark (21 Spender, Alter 11,76) und von Spongiosa aus Spendermaterial (32 Spender, Alter 13,84). Das Alter hatte keinen signifikanten Einfluss auf die erfolgreiche Isolierung und Kultivierung von hMSCs, obgleich die Misserfolgsrate bei Spendern über 60 Jahren 55.6% betrug, währenddem sie bei Spendern unter 60 Jahren zwischen 14.3% und 22.2% variierte. Männer und Frauen hatten vergleichbare Misserfolgsraten (27.3%, beziehungsweise 28.6%). Die Kultivierung von hMSCs war bei 90.4% des aspirierten Marks von 21 Spendern und 62.5% der Spongiosa von 35 Spendern erfolgreich. Diese Differenz war statistisch signifikant (P=0.023). Die Regressionsanalyse bestätigte, dass von den drei gleichzeitig getesteten Variablen nur die Entnahmequelle der Zellen das Resultat signifikant beeinflusste (P=0.043). Die morphologische Untersuchung der unfraktionierten Primärpopulation zeigte bei den adhärenten Zellen eine Veränderung der Zellform von einer dreieckigen zu einer spindelförmigen länglichen Form. Diese blieb bis zu ihrem Zusammenwachsen erhalten. Wurden die Kulturen einem osteoinduktiven Einfluss ausgesetzt, entwickelten sich verschiedene Morphotypen, die auch verschiedene Mengen alkalischer Phosphatase und Mineralien ausschieden. Die Morphologie der MSCs aus dem aspirierten Knochenmark war nicht anders als diejenige von MSCs aus spongiösen Knochenproben. Die Ausscheidung von osteogenen Markern durch die MSCs, auch messbar durch den PCR, verhielt sich bei den Zellen aus beiden Quellen gleich. Man schloss daher, dass aspiriertes Knochenmark und Spongiosa vergleichbare Populationen von MSCs produzieren. Für die Isolation und Expansion von hMSCs zur gesteuerten Knochengewebsregeneration ist aspiriertes Knochenmark vorteilhafter als spongiöse Knochenchips und der Spendern ist von Vorteil jünger als 60 Jahre. Resumen La intención de este estudio fue valorar el efecto de las características del donante y de la técnica de recogida y del procesamiento in vivo sobre la eficacia del cultivo de células madre mesenquimales humanas (hMSCs) para crecimiento óseo por ingeniería tisular. Los cultivos de hMSCs se derivaron de aspirados de la médula ósea de la cresta iliaca (21 donantes, edad 11,76) y de material de injerto de hueso esponjoso (32 donantes, edad 13,84). La edad no tuvo un efecto significativo en la habilidad para aislar y cultivar hMSCs, aunque el índice de fracaso fue del 55.6% en donantes por encima de los 60 años, mientras que varió entre el 14.3% y el 22.2% en donantes por debajo de los 60 años. Los donantes varones y hembras tuvieron índices de fracaso comparables (27.3 y 28.6%, respectivamente). El cultivo de hMSCs tuvo éxito en el 90.4% de los aspirados medulares de 21 donantes y en 62.5% de los especímenes de hueso esponjoso de 35 donantes. Esta diferencia fue estadísticamente significativa (P=0.023). El análisis de regresión confirmó que en la prueba simultanea de las tres variables, solo el origen de las células afectó significativamente el resultado (P=0.043). La evaluación morfológica de la población primaria no fraccionada mostró un cambio en la forma celular de las células adherentes de triangular a una forma de huso fino elongado, que se mantiene hasta su confluencia. Cuando los cultivos se expusieron a un medio osteoconductivo se evidenciaron varios morfotipos manifestando diferentes niveles de fosfatasa alcalina y segregando diferentes cantidades de mineral. La morfología de las MSCs de los aspirados medulares no fue diferente de las MSCs derivadas de los especímenes de hueso esponjoso. La manifestación de marcadores osteogénicos en MSCs mostrado por PCR tampoco difirió entre las dos fuentes. Se concluye que los aspirados medulares y los especímenes de hueso esponjoso producen poblaciones comparables de MSCs. De todos modos, los aspirados de médula ósea de donantes menores de 60 años por encima de las virutas de hueso esponjoso son más favorables para el aislamiento y la expansión de hMSCs para crecimiento óseo por ingeniería tisular. [source] Load-related implant reaction of mini-implants used for orthodontic anchorageCLINICAL ORAL IMPLANTS RESEARCH, Issue 4 2005André Büchter Abstract: The purpose of this study was to determine the clinical and biomechanical outcome of two different titanium mini-implant systems activated with different load regimens. A total of 200 mini-implants (102 Abso Anchor® and 98 Dual Top®) were placed in the mandible of eight Göttinger minipigs. Two implants each were immediately loaded in opposite direction by various forces (100, 300 or 500 cN) through tension coils. Additionally, three different distances between the neck of the implant and the bone rim (1, 2 and 3 mm) were used. The different load protocols were chosen to evaluate the load-related implant performance. The load was provided by superelastic tension coils, which are known to develop a virtually constant force. Non-loaded implants were used as a reference. Following an experimental loading period of 22 and 70 days half of the minipigs were sacrificed, and implant containing bone specimens evaluated for clinical performance and implant stability. Implant loosing was found to be statistically dependent on the tip moment (TM) at the bone rim. Clinical implant loosing were only present when load exceeded 900 cN mm. No movement of implants through the bone was found in the experimental groups, for any applied loads. Over the two experimental periods the non-loaded implants of one type of implant had a higher stability than those of the loaded implants. Dual Top® implants revealed a slightly higher removal torque compared with Abso Anchor® implants. Based on the results of this study, immediate loading of mini-implants can be performed without loss of stability when the load-related biomechanics do not exceed an upper limit of TM at the bone rim. Résumé Le but de cette étude a été de déterminer ce qui se passait cliniquement et biomécaniquement au niveau de deux systèmes de mini-implants en titane activés par différents régimes de mise en charge. Deux cents mini-implants (102 Abso Anchor® et 98 Dual Top®) ont été placés dans la mandibule de huit mini-porcs de Göttinger. Deux implants chacun ont été immédiatement mis en charge dans une direction opposée avec des forces variables (100 cN, 300 cN ou 500 cN) par des ressorts de tension. De plus trois distances différentes entre l'épaulement de l'implant et le rebord osseux (1, 2, 3 mm) ont été utilisés. Les différents protocoles de mise en charge ont été choisis pour évaluer la performance implantaire vis-à-vis de la mise en charge. Cette charge était produite par des ressorts de tension super-élastiques connues pour développer une force quasi-constante. Des implants sans charge ont servi de contrôle. Suivant une période de mise en charge de 22 et 70 jours la moitié des mini-porcs ont été euthanasiés et les spécimens comprenant l'os et l'implant évalués pour leur performance clinique et leur stabilité implantaire. La mobilité implantaire a été reconnue comme statisquement dépendante du moment de l'extrêmité au niveau du bord osseux. La mobilité implantaire clinique a seulement été rencontrée lorsque la charge dépassait 900 cN mm. Aucun mouvement des implants n'a été trouvée dans le groupe expérimental pour aucune des forces appliquées. Durant les deux périodes expérimentales les implants non-chargés d'un type d'implant avaient une stabilité supérieure à celle des implants chargés. Les implants Dual Top® avaient une force de torsion d'enlèvement un peu plus importante que les implants Abso Anchor®. La mise en charge immédiate de mini-implants peut être effectuée sans perte de stabilité lorsque la charge en relation avec la biomécanique n'excède pas une limite supérieure du moment de l'extrêmité au niveau du bord osseux. Zusammenfassung Es war das Ziel dieser Studie, die klinische und biomechanische Reaktion bei zwei verschiedenen Titan Mini-Implantat-Systemen, welche verschiedenen Belastungen ausgesetzt wurden, zu bestimmen. Insgesamt wurden 200 Mini-Implantate (102 Abso Anchor® und 98 Dual Top®) in die Unterkiefer von 8 Göttinger Minipigs eingesetzt. Immer zwei Implantate wurden jeweils sofort in entgegen gesetzter Richtung mit unterschiedlichen Kräften (100 cN, 300 cN oder 500 cN) durch Zugfedern belastet. Zusätzlich wurden drei verschiedene Abstände zwischen dem Hals des Implantats und der Knochenkante (1 mm, 2 mm, 3 mm) verwendet. Die unterschiedlichen Belastungsprotokolle wurden ausgewählt, um die belastungsbedingte Implantatreaktion auszuwerten. Die Belastung wurde mit superelastischen Zugfedern durchgeführt, welche dafür bekannt sind, dass sie eine relativ konstante Kraft entwickeln. Unbelastete Implantate dienten als Kontrolle. Nach einer experimentellen Belastungsdauer von 22 und 70 Tagen wurde je die Hälfte der Minipigs geopfert. An Knochenpräparaten mit Implantaten wurde die klinische Reaktion und die Stabilität der Implantate ausgewertet. Es wurde entdeckt, dass die Lockerung von Implantaten statistisch signifikant abhängig vom Kippmoment auf Höhe der Knochenkante war. Eine klinische Lockerung von Implantaten trat nur auf, wenn die Belastung 900 cN mm überstieg. Bei den experimentellen Gruppen konnte mit den applizierten Kräften keine Bewegung der Implantate durch den Knochen ausgelöst werden. Ueber die zwei Abschnitte des Experiments wiesen die nicht belasteten Implantate eines Typs eine grössere Stabilität als die belasteten Implantate auf. Die Dual Top® Implantate zeigten leicht höhere Ausdrehmomente als die Abso Anchor® Implantate. Aufgrund der Resultate dieser Studie kann eine Sofortbelastung von Mini-Implantaten ohne Stabilitätsverlust durchgeführt werden, sofern durch die Belastung die obere Grenze eines Kippmoments auf Höhe der Knochenkante nicht überschreitet. Resumen El propósito de este estudio fue determinar los resultados clínicos y biomecánicos de dos sistemas diferentes de mini-implantes de titanio activados con diferentes regimenes de carga. Se colocaron un total de 200 mini-implantes (102 Abso Anchor® y 98 Dual Top®) en la mandíbula de 8 minicerdos Göttinger. Se cargaron dos implantes inmediatamente en direcciones opuestas con varias fuerzas (100 cN, 300 cN o 500 cN) a través de resortes de tensión. Se usaron, adicionalmente, tres distancias diferentes entre el cuello del implante y el reborde óseo (1 mm, 2 mm, 3 mm). Los diferentes protocolos de carga se eligieron para evaluar el rendimiento relacionado con la carga. La carga se suministró por medio de resortes de tensión superelásticos, conocidos por desarrollar una fuerza virtualmente constante. Los implantes no cargados se usaron como referencia. Tras un periodo experimental de carga de 22 y 70 días la mitad de los minicerdos se sacrificaron, y se evaluaron los implantes conteniendo especímenes óseos para el rendimiento clínico y estabilidad de los implantes. El aflojamiento del implante se encontró que era estadísticamente dependiente de la punta del momento en el reborde óseo. El aflojamiento clínico del implante solo se presentó cuando la carga excedió los 900 cN mm. No se encontró movimiento de los implantes a través del hueso en los grupos experimentales, en ninguna de las cargas aplicadas. A lo largo de los dos periodos experimentales los implantes sin carga de un tipo de implante tuvieron una mayor estabilidad que aquellos de los implantes cargados. Los implantes Dual Top® necesitaron un mayor torque de remoción en comparación con los implantes Abso Anchor®. Basándose en los resultados de este estudio, la carga inmediata se puede llevar a cabo sin pérdida de estabilidad cuando la biomecánica relacionada con la carga no supere un límite superior de la punta del momento en el reborde óseo. [source] | |||||||||||||